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Your search keyword '"Cai, Mei-Qiang"' showing total 32 results
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32 results on '"Cai, Mei-Qiang"'

31. An assay for identification and determination of toxic rodenticide valone in serum by ion chromatography–electrospray ionization tandem mass spectrometry with ion trap detector

Author

Cai, Mei-Qiang, Dong, Xin-Yan, Chen, Xiao-Hong, and Jin, Mi-Cong

Subjects
*POISON analysis, *RODENTICIDES, *ANTICOAGULANTS, *SERUM, *CHROMATOGRAPHIC analysis, *ELECTROSPRAY ionization mass spectrometry, *ION traps, *CHEMICAL detectors
Abstract

Abstract: Valone has a chronic and toxic anticoagulant rodenticide that has widely used in China and has resulted in some accidental and intentional intoxication in recent years. The literature reported so far lacks sensitive and selective method for the confirmation of valone. The purpose of this study was to establish a novel assay for the identification and quantification of valone in serum by ion chromatography–electrospray ionization tandem mass spectrometry (IC–MS/MS). After serum sample was extracted with methanol/acetonitrile (10:90, v/v) and cleaned by Oasis® HLB solid-phase extraction cartridge, chromatographic separation was performed on an Ionpac® AS11 column with an eluent of methanol/30mmol/L KOH (10:90, v/v). The overall extraction efficiency was >81.0%, and the limit of quantification was 0.5ng/mL for valone. Regression analysis of the calibration data revealed good correlation (r 2 >0.99) for valone. Intra- and inter-day precisions for quality-control samples were less than 8.0 and 13.7%, respectively. The proposed method enables the identification and quantification of valone in both clinical and forensic specimens. [Copyright &y& Elsevier]

Published
2009
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32. Rapid identification and determination of the rodenticide valone in serum by high-performance liquid chromatography-tandem mass spectrometry.

Author

Cai MQ, Chen XH, Ouyang XK, and Jin MC

Subjects
Adult, Butanols poisoning, Female, Humans, Indenes poisoning, Male, Poisoning blood, Reference Standards, Reproducibility of Results, Rodenticides poisoning, Sensitivity and Specificity, Time Factors, Young Adult, Butanols blood, Chromatography, High Pressure Liquid methods, Indenes blood, Rodenticides blood, Tandem Mass Spectrometry methods
Abstract

Valone is a chronic anticoagulant rodenticide that has come into wide use in China. Current literature lacks analytical methods for the determination of valone. In this paper, a sensitive and selective assay was established for the identification and quantification of valone in serum by liquid chromatography-tandem mass spectrometry. After addition of the internal standard, warfarin, serum samples were extracted with 10% methanol in acetonitrile and cleaned using Oasis HLB solid-phase extraction (SPE) cartridges. The compounds were separated on an Agilent SB C18 column with a mobile phase of methanol/acetic acid-ammonium acetate (5 mmol/L, pH 6.3) (75:25, v/v). Detection was performed by electrospray ionization ion trap mass spectrometry in the negative multiple reaction monitoring mode. The transition ions of m/z 229 --> 145 and m/z 307 --> 161 were selected for quantification of valone and the internal standard, respectively. The overall extraction efficiency was between 81.1% and 91.1%. The limit of quantification was 0.5 ng/mL. Regression analysis of the calibration data revealed good correlation (r(2) > 0.99) for valone. Intra- and interday precisions for quality-control samples were less than 7.8% and 12.8%, respectively. This method combines a rapid SPE procedure with an extremely fast chromatographic analysis, which is especially advantageous or clinical laboratories.

Published
2009
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