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329 results on '"Cai, Zhaoxia"'

30. Optimizing the Encapsulation Behavior of Egg Yolk on DHA by Vacuum Low-Temperature Spray Drying to Improve the Hydration Properties of the Powder.

Author

Sun, Haoyang, Mourad, Fayez Khalaf, Chen, Nan, Zhang, Xinyue, Li, Qiqi, Li, Xiaomeng, Ding, Lixian, Dong, Wanyi, Huang, Xi, Shu, Dewei, Wang, Zhiyuan, and Cai, Zhaoxia

Subjects
SPRAY drying, EGG yolk, HYDRATION, DOCOSAHEXAENOIC acid, HYDROGEN bonding, FISH oils, POWDERS
Abstract

Due to the poor aqueous solubility of docosahexaenoic acid (DHA), encapsulating it in egg yolk results in a reduction in the hydration properties of egg yolk powder (EYP), subsequently affecting its functional properties. In this study, the effects of vacuum low-temperature spray drying (VLTSD), high-temperature spray drying (HTSD), and freeze-drying (FD) on the hydration properties of DHA-enriched EYP were investigated. Firstly, we optimized the inlet temperature and feed rate for VLTSD (60 °C, 300 mL/h) and HTSD (170 °C, 800 mL/h), while FD was conducted under − 70 °C conditions. The most striking finding of this study was the DHA-enriched EYP prepared by VLTSD exhibited desirable solubility (54.86 g/100 g), water dispersibility (48.63%), and coefficient of stability (24.88%) during rehydration, which was well predicted by low-field NMR. Compared to HTSD and FD, the solubility increased by 16.18% and 45.17%, respectively. Furthermore, at the microscopic level, SEM images and FTIR spectra revealed that VLTSD enhanced the hydration properties by protecting the structural integrity and increasing the hydrogen bonding of the DHA-enriched EYP. The encapsulation efficiency of DHA-enriched EYP prepared by VLTSD, HTSD, and FD were 97.15%, 96.51%, and 81.31%, respectively. According to the quality analysis, VLTSD had the best protection for DHA-enriched EYP in terms of chromaticity, bulk density, storage stability, and sensory evaluation. Therefore, this study confirmed the applicability of VLTSD technology for microencapsulation of bioactive substances such as DHA to enhance the hydration properties of powders. [ABSTRACT FROM AUTHOR]

Published
2024
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35. PMS1T, producing phased small-interfering RNAs, regulates photoperiod-sensitive male sterility in rice.

Author

Fan, Yourong, Yang, Jiangyi, Mathioni, Sandra, Yu, Jinsheng, Shen, Jianqiang, Yang, Xuefei, Wang, Lei, Zhang, Qinghua, Cai, Zhaoxia, Xu, Caiguo, Li, Xianghua, Xiao, Jinghua, Meyers, Blake, and Zhang, Qifa

Subjects
long-noncoding RNA, phasiRNA, photoperiod-sensitive male sterility, Chromosome Mapping, Cloning, Molecular, Gene Expression Profiling, Gene Expression Regulation, Plant, Genes, Plant, Genetic Loci, Genetic Markers, Open Reading Frames, Oryza, Phenotype, Photoperiod, Plant Infertility, Plant Proteins, Plants, Genetically Modified, Polymorphism, Single Nucleotide, RNA Interference, RNA, Long Noncoding, RNA, Plant, RNA, Small Interfering
Abstract

Phased small-interfering RNAs (phasiRNAs) are a special class of small RNAs, which are generated in 21- or 24-nt intervals from transcripts of precursor RNAs. Although phasiRNAs have been found in a range of organisms, their biological functions in plants have yet to be uncovered. Here we show that phasiRNAs generated by the photopheriod-sensetive genic male sterility 1 (Pms1) locus were associated with photoperiod-sensitive male sterility (PSMS) in rice, a germplasm that started the two-line hybrid rice breeding. The Pms1 locus encodes a long-noncoding RNA PMS1T that was preferentially expressed in young panicles. PMS1T was targeted by miR2118 to produce 21-nt phasiRNAs that preferentially accumulated in the PSMS line under long-day conditions. A single nucleotide polymorphism in PMS1T nearby the miR2118 recognition site was critical for fertility change, likely leading to differential accumulation of the phasiRNAs. This result suggested possible roles of phasiRNAs in reproductive development of rice, demonstrating the potential importance of this RNA class as regulators in biological processes.

Published
2016

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