1. Antibiofilm efficacy of a calcium silicate-based intracanal medicament against Fusobacterium nucleatum strains.
- Author
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Balto H, Barakat R, Basudan S, Fakeeha G, Alharbi SR, and Almohareb R
- Subjects
- Humans, Microbial Viability drug effects, Dentin microbiology, Microscopy, Confocal, Calcium Hydroxide pharmacology, Root Canal Irrigants pharmacology, Fusobacterium nucleatum drug effects, Fusobacterium nucleatum physiology, Biofilms drug effects, Biofilms growth & development, Silicates pharmacology, Calcium Compounds pharmacology, Anti-Bacterial Agents pharmacology
- Abstract
This study evaluated the antibiofilm efficacy of a calcium silicate-based intracanal medicament (Bio-C Temp) against Fusobacterium nucleatum (F. nucleatum). Dentin slices (n = 88) were inoculated with F. nucleatum biofilms and divided into experimental groups (n = 24 each) treated with Bio-C Temp, triple antibiotic paste (TAP), or Ca(OH)
2 for 1 or 2 weeks. Untreated dentin slices exposed to saline served as a positive control, while sterile dentin slices incubated anaerobically served as a negative control. Bacterial viability was assessed using confocal laser scanning microscopy after staining with LIVE/DEAD® BacLight™ dye. Samples treated with TAP demonstrated the highest percentage of dead bacteria (95%). The antibiofilm effect of TAP and Bio-C Temp significantly increased from Week 1 to Week 2. At Week 2, samples treated with Bio-C Temp showed a significantly higher percentage of dead bacteria (75.25%) than those treated with Ca(OH)2 (58.65%). TAP showed superior efficacy against F. nucleatum biofilms. After an application time of 2 weeks, the antibiofilm efficacy of Bio-C Temp was superior to that of Ca(OH)2 ., (© 2024. The Author(s).)- Published
- 2024
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