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3. First step results from a phase II study of a dendritic cell vaccine in glioblastoma patients (CombiG-vax).

Author

Ridolfi, Laura, Gurrieri, Lorena, Riva, Nada, Bulgarelli, Jenny, De Rosa, Francesco, Guidoboni, Massimo, Fausti, Valentina, Ranallo, Nicoletta, Calpona, Sebastiano, Tazzari, Marcella, Petrini, Massimiliano, Granato, Anna Maria, Pancisi, Elena, Foca, Flavia, D'Allagata, Monia, Bondi, Isabella, Amadori, Elena, Cortesi, Pietro, Zani, Chiara, and Ancarani, Valentina

Subjects
CYTOTOXIC T cells, DENDRITIC cells, PROGRESSION-free survival, CELLULAR therapy, SKIN tests, LEUKAPHERESIS
Abstract

Background: Glioblastoma (GBM) is a poor prognosis grade 4 glioma. After surgical resection, the standard therapy consists of concurrent radiotherapy (RT) and temozolomide (TMZ) followed by TMZ alone. Our previous data on melanoma patients showed that Dendritic Cell vaccination (DCvax) could increase the amount of intratumoral-activated cytotoxic T lymphocytes. Methods: This is a single-arm, monocentric, phase II trial in two steps according to Simon's design. The trial aims to evaluate progression-free survival (PFS) at three months and the safety of a DCvax integrated with standard therapy in resected GBM patients. DCvax administration begins after completion of RTCTwith weekly administrations for 4 weeks, then is alternated monthly with TMZ cycles. The primary endpoints are PFS at three months and safety. One of the secondary objectives is to evaluate the immune response both in vitro and in vivo (DTH skin test). Results: By December 2022, the first pre-planned step of the study was concluded with the enrollment, treatment and follow up of 9 evaluable patients. Two patients had progressed within three months after leukapheresis, but none had experienced DCvax-related G3-4 toxicities Five patients experienced a positive DTH test towards KLH and one of these also towards Frontiers autologous tumor homogenate. The median PFS from leukapheresis was 11.3 months and 12.2 months from surgery. Conclusions: This combination therapy is well-tolerated, and the two endpoints required for the first step have been achieved. Therefore, the study will proceed to enroll the remaining 19 patients. [ABSTRACT FROM AUTHOR]

Published
2024
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5. The potential role of the extracellular matrix in the activity of trabectedin in UPS and L-sarcoma: evidences from a patient‐derived primary culture case series in tridimensional and zebrafish models

Author

De Vita, Alessandro, Recine, Federica, Miserocchi, Giacomo, Pieri, Federica, Spadazzi, Chiara, Cocchi, Claudia, Vanni, Silvia, Liverani, Chiara, Farnedi, Anna, Fabbri, Francesco, Fausti, Valentina, Casadei, Roberto, Brandolini, Francesca, Ercolani, Giorgio, Cavaliere, Davide, Bongiovanni, Alberto, Riva, Nada, Gurrieri, Lorena, Di Menna, Giandomenico, Calpona, Sebastiano, Debonis, Silvia Angela, Mercatali, Laura, and Ibrahim, Toni

Published
2021
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6. High-grade transformation of a polymorphous adenocarcinoma of the salivary gland: a case report and review of the literature

Author

Miserocchi, Giacomo, primary, Bassi, Massimo, additional, De Luca, Giovanni, additional, Calpona, Sebastiano, additional, De Rosa, Francesco, additional, Bongiovanni, Alberto, additional, Parisi, Elisabetta, additional, Di Menna, Giandomenico, additional, De Vita, Alessandro, additional, Liverani, Chiara, additional, Spadazzi, Chiara, additional, Cocchi, Claudia, additional, Vanni, Silvia, additional, Capelli, Laura, additional, Magnani, Massimo, additional, Meccariello, Giuseppe, additional, Vicini, Claudio, additional, Campobassi, Angelo, additional, Mercatali, Laura, additional, and Ibrahim, Toni, additional

Published
2023
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7. Systemic Inflammatory Indices in Second-Line Soft Tissue Sarcoma Patients: Focus on Lymphocyte/Monocyte Ratio and Trabectedin

Author

Fausti, Valentina, primary, De Vita, Alessandro, additional, Vanni, Silvia, additional, Ghini, Virginia, additional, Gurrieri, Lorena, additional, Riva, Nada, additional, Casadei, Roberto, additional, Maraldi, Marco, additional, Ercolani, Giorgio, additional, Cavaliere, Davide, additional, Pacilio, Carlo Alberto, additional, Pieri, Federica, additional, Foca, Flavia, additional, Bongiovanni, Alberto, additional, Ranallo, Nicoletta, additional, Calpona, Sebastiano, additional, Frassineti, Giovanni Luca, additional, Ibrahim, Toni, additional, and Mercatali, Laura, additional

Published
2023
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9. Precision Medicine in Head and Neck Cancers: Genomic and Preclinical Approaches

Author

Miserocchi, Giacomo, primary, Spadazzi, Chiara, additional, Calpona, Sebastiano, additional, De Rosa, Francesco, additional, Usai, Alice, additional, De Vita, Alessandro, additional, Liverani, Chiara, additional, Cocchi, Claudia, additional, Vanni, Silvia, additional, Calabrese, Chiara, additional, Bassi, Massimo, additional, De Luca, Giovanni, additional, Meccariello, Giuseppe, additional, Ibrahim, Toni, additional, Schiavone, Marco, additional, and Mercatali, Laura, additional

Published
2022
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10. Deciphering the Genomic Landscape and Pharmacological Profile of Uncommon Entities of Adult Rhabdomyosarcomas

Author

De Vita, Alessandro, primary, Vanni, Silvia, additional, Fausti, Valentina, additional, Cocchi, Claudia, additional, Recine, Federica, additional, Miserocchi, Giacomo, additional, Liverani, Chiara, additional, Spadazzi, Chiara, additional, Bassi, Massimo, additional, Gessaroli, Manlio, additional, Campobassi, Angelo, additional, De Luca, Giovanni, additional, Pieri, Federica, additional, Farnedi, Anna, additional, Franchini, Eugenia, additional, Ferrari, Anna, additional, Domizio, Chiara, additional, Cavagna, Enrico, additional, Gurrieri, Lorena, additional, Bongiovanni, Alberto, additional, Riva, Nada, additional, Calpona, Sebastiano, additional, Di Menna, Giandomenico, additional, Debonis, Silvia Angela, additional, Ibrahim, Toni, additional, and Mercatali, Laura, additional

Published
2021
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11. Additional file 2 of The potential role of the extracellular matrix in the activity of trabectedin in UPS and L-sarcoma: evidences from a patient‐derived primary culture case series in tridimensional and zebrafish models

Author

De Vita, Alessandro, Recine, Federica, Miserocchi, Giacomo, Pieri, Federica, Spadazzi, Chiara, Cocchi, Claudia, Vanni, Silvia, Liverani, Chiara, Farnedi, Anna, Fabbri, Francesco, Fausti, Valentina, Casadei, Roberto, Brandolini, Francesca, Ercolani, Giorgio, Cavaliere, Davide, Bongiovanni, Alberto, Riva, Nada, Gurrieri, Lorena, Di Menna, Giandomenico, Calpona, Sebastiano, Debonis, Silvia Angela, Mercatali, Laura, and Ibrahim, Toni

Abstract

Additional file 2: Supplementary methods.

Published
2021
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13. Hemangioblastoma of the Gastrointestinal Tract: A First Case

Author

Gardini, Andrea Casadei, Pieri, Federica, Fusaroli, Pietro, Oboldi, Devil, Passardi, Alessandro, Monti, Manlio, Rosetti, Paola, Calpona, Sebastiano, Valgiusti, Martina, Ragazzini, Angela, Amadori, Dino, and Frassineti, Giovanni Luca

Published
2013

14. Three-dimensional collagen-based scaffold model to study the microenvironment and drug-resistance mechanisms of oropharyngeal squamous cell carcinomas

Author

Miserocchi, Giacomo, primary, Cocchi, Claudia, additional, De Vita, Alessandro, additional, Liverani, Chiara, additional, Spadazzi, Chiara, additional, Calpona, Sebastiano, additional, Di Menna, Giandomenico, additional, Bassi, Massimo, additional, Meccariello, Giuseppe, additional, De Luca, Giovanni, additional, Campobassi, Angelo, additional, Maddalena Tumedei, Maria, additional, Bongiovanni, Alberto, additional, Fausti, Valentina, additional, Cotelli, Franco, additional, Ibrahim, Toni, additional, and Mercatali, Laura, additional

Published
2021
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15. Temozolomide Alone or Combined with Capecitabine for the Treatment of Metastatic Neuroendocrine Neoplasia: A “Real-World” Data Analysis

Author

Bongiovanni, Alberto, primary, Liverani, Chiara, additional, Foca, Flavia, additional, Fausti, Valentina, additional, Di Menna, Giandomenico, additional, Mercatali, Laura, additional, De Vita, Alessandro, additional, Riva, Nada, additional, Calpona, Sebastiano, additional, Miserocchi, Giacomo, additional, Spadazzi, Chiara, additional, Cocchi, Claudia, additional, and Ibrahim, Toni, additional

Published
2020
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16. Abstract 333: Development of a biomimetic 3D scaffold to study breast cancer bone metastasis

Author

Spadazzi, Chiara, primary, Mercatali, Laura, additional, De Vita, Alessandro, additional, Liverani, Chiara, additional, Cocchi, Claudia, additional, Miserocchi, Giacomo, additional, Bongiovanni, Alberto, additional, Recine, Federica, additional, Calpona, Sebastiano, additional, Riva, Nada, additional, Menna, Giandomenico Di, additional, Gurrieri, Lorena, additional, Fausti, Valentina, additional, De Bonis, Silvia Angela, additional, Martinelli, Giovanni, additional, Baldini, Nicola, additional, and Ibrahim, Toni, additional

Published
2020
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17. Establishment of a primary cell culture of bone metastasis from urothelial carcinoma.

Author

Spadazzi, Chiara, primary, Cocchi, Claudia, additional, Casadei, Roberto, additional, Brandolini, Francesca, additional, Pieri, Federica, additional, Bongiovanni, Alberto, additional, Recine, Federica, additional, Liverani, Chiara, additional, De Vita, Alessandro, additional, Miserocchi, Giacomo, additional, Calpona, Sebastiano, additional, Debonis, Silvia Angela, additional, Ibrahim, Toni, additional, and Mercatali, Laura, additional

Published
2020
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19. Identification of a novel RAB3IP‐HMGA2 fusion transcript in an adult head and neck rhabdomyosarcoma.

Author

De Vita, Alessandro, Ferrari, Anna, Miserocchi, Giacomo, Vanni, Silvia, Domizio, Chiara, Fonzi, Eugenio, Fausti, Valentina, Recine, Federica, Bassi, Massimo, Campobassi, Angelo, Liverani, Chiara, Spadazzi, Chiara, Cocchi, Claudia, De Luca, Giovanni, Pieri, Federica, Gurrieri, Lorena, Di Menna, Giandomenico, Calpona, Sebastiano, Bongiovanni, Alberto, and Martinelli, Giovanni

Subjects
PROTEINS, SEQUENCE analysis, GENETIC mutation, RHABDOMYOSARCOMA, IMMUNOHISTOCHEMISTRY, HEAD & neck cancer, CELL physiology, BIOINFORMATICS, TREATMENT effectiveness, GENE rearrangement, CELL proliferation, TRANSCRIPTION factors, CELL lines
Abstract

The article describes the case of a 74-year old patient diagnosed with RAB3IP-HMGA2 gene fusion transcript in head and neck rhabdomyosarcoma. Results of immunohistochemical examinations showed positivity of tumor cells for vimentin, MyoD1, focal positivity for desmin, myoglobin, myogenin, CD34 and AE1-AE3 cytokeratin and negativity for S100, STAT6 and P63. Findings also indicates the association of RAB3IP-HMGA2 with tumor cell proliferation and epithelial mesenchymal transition (EMT).

Published
2022
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20. BOne HEalth ManagEment in Patients with Early Breast Cancer: A Retrospective Italian Osteoncology Center “Real-Life” Experience (BOHEME Study)

Author

Recine, Federica, primary, Bongiovanni, Alberto, additional, Foca, Flavia, additional, Mercatali, Laura, additional, Fausti, Valentina, additional, Calpona, Sebastiano, additional, Riva, Nada, additional, Vita, Alessandro De, additional, Liverani, Chiara, additional, Spadazzi, Chiara, additional, Miserocchi, Giacomo, additional, Di Menna, Giandomenico, additional, Gurrieri, Lorena, additional, Cocchi, Claudia, additional, Debonis, Silvia Angela, additional, Vespignani, Roberto, additional, and Ibrahim, Toni, additional

Published
2019
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21. The emerging role of trans-oral robotic surgery for the detection of the primary tumour site in patients with head-neck unknown primary cancers: A meta-analysis

Author

Meccariello, Giuseppe, primary, Cammaroto, Giovanni, additional, Ofo, Enyinnaya, additional, Calpona, Sebastiano, additional, Parisi, Elisabetta, additional, D’Agostino, Giovanni, additional, Gobbi, Riccardo, additional, Firinu, Elisabetta, additional, Bellini, Chiara, additional, De Vito, Andrea, additional, Montevecchi, Filippo, additional, Costantini, Matteo, additional, Amadori, Elena, additional, Nuzzo, Simona, additional, Pelucchi, Stefano, additional, and Vicini, Claudio, additional

Published
2019
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22. Clinical role of filgrastim in the management of patients at risk of prolonged severe neutropenia: An evidence‐based review

Author

Bongiovanni, Alberto, primary, Recine, Federica, additional, Fausti, Valentina, additional, Rossi, Benedetta, additional, Mercatali, Laura, additional, Liverani, Chiara, additional, De Vita, Alessandro, additional, Gurrieri, Lorena, additional, De Bonis, Silvia, additional, Miserocchi, Giacomo, additional, Spadazzi, Chiara, additional, Calpona, Sebastiano, additional, Riva, Nada, additional, Cocchi, Claudia, additional, and Ibrahim, Toni, additional

Published
2019
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23. Evaluation of prognostic role of inflammatory index in glioblastoma multiforme patients undergoing to concomitant radio-chemotherapy.

Author

Fausti, Valentina, primary, Foca, Flavia, additional, Riva, Nada, additional, Bongiovanni, Alberto, additional, Gurrieri, Lorena, additional, Di Menna, Giandomenico, additional, Calpona, Sebastiano, additional, De Bonis, Silvia Angela, additional, Mercatali, Laura, additional, Spadazzi, Chiara, additional, De Vita, Alessandro, additional, Liverani, Chiara, additional, Miserocchi, Giacomo, additional, Cocchi, Claudia, additional, Dall'Agata, Monia, additional, Tosatto, Luigino, additional, Romeo, Antonino, additional, Amadori, Dino, additional, Martinelli, Giovanni, additional, and Ibrahim, Toni, additional

Published
2019
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24. Temozolomide Alone or Combined with Capecitabine for the Treatment of Metastatic Neuroendocrine Neoplasia: A "Real-World" Data Analysis.

Author

Bongiovanni, Alberto, Liverani, Chiara, Foca, Flavia, Fausti, Valentina, Di Menna, Giandomenico, Mercatali, Laura, De Vita, Alessandro, Riva, Nada, Calpona, Sebastiano, Miserocchi, Giacomo, Spadazzi, Chiara, Cocchi, Claudia, and Ibrahim, Toni

Subjects
TEMOZOLOMIDE, OVERALL survival, DATA analysis, NEUROENDOCRINE tumors, PROGNOSIS, ENTEROCOLITIS
Abstract

Background: Neuroendocrine neoplasias (NENs) are a rare group of tumors with different prognosis and response to therapy. Their heterogeneity is dependent on the site of origin, morphology, and Ki67. Temozolomide (TEM) appears to be active in metastatic NENs (mNENs) but there is limited evidence about its efficacy in gastrointestinal NENs. We analyzed "real-world" data on the use of TEM alone or in association with capecitabine (CAPTEM) in patients with mNENs. Patients and Methods: One hundred consecutive patients with advanced NENs treated with TEM or CAPTEM between 2009 and 2019 were included. A pretreatment tumor growth rate (TGR0) was calculated. Overall survival (OS), progression-free survival (PFS), tolerance, objective response rate (ORR), and disease control rate (DCR) were analyzed. A propensity score analysis and inverse probability of treatment weights for Cox regression models were used. Results: TEM-based therapy was administered to 95 patients (26.3% CAPTEM and 83.7% TEM) with a median age of 59 years (range 26–85) years. ECOG performance status was 0–2. Carcinoid syndrome was reported in 12 (12.6%) patients. Twenty (21.1%) patients with grade (G) 3 neuroendocrine carcinoma (NEC) and 9 (9.4%) with G3 neuroendocrine tumors (NETs) were included in the analysis. Median PFS of the entire group was 10.4 months (95% confidence interval [CI]: 6.0–11.5). In multivariate analysis, a higher risk of progression was observed for NEC G3 patients (hazard ratio [HR] 2.70, 95% CI: 1.25–5.84) and for a TGR ≥19.55 (HR: 2.53, 95% CI: 1.45–4.40). Median OS was 23.4 months (95% CI: 17.0–29.0) and was similar in both treatment groups (23.9 vs. 20.5 months for TEM and CAPTEM, respectively, p = 0.585). In multivariate analysis, TGR ≥19.55 was associated with a higher risk of death (HR: 2.18, 95% CI: 1.16–4.11) than TGR <19.55, as was NEC G3 (HR: 2.42, 95% CI: 1.04–5.59) with respect to NETs. No differences in terms of mPFS or mOS were seen in relation to the primary site of disease. In the 86 patients evaluable for response, ORR was 44.1% and the DCR was 70.9%. Mild adverse events (grade I–II) included anemia, neutropenia, and headache. Rare cases of G 3 neutropenia and thrombocytopenia were recorded. Conclusions: TEM-based regimens are associated with a high DCR and a relatively tolerable toxicity profile in NENs of pancreatic, intestinal, and lung origin. Further investigation of these specific NETs is warranted in prospective clinical trials. [ABSTRACT FROM AUTHOR]

Published
2021
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25. Three-dimensional collagen-based scaffold model to study the microenvironment and drug-resistance mechanisms oforopharyngeal squamous cell carcinomas.

Author

Miserocchi, Giacomo, Cocchi, Claudia, De Vita, Alessandro, Liverani, Chiara, Spadazzi, Chiara, Calpona, Sebastiano, Menna, Giandomenico Di, Bassi, Massimo, Meccariello, Giuseppe, De Luca, Giovanni, Campobassi, Angelo, Tumedei, Maria Maddalena, Bongiovanni, Alberto, Fausti, Valentina, Cotelli, Franco, ToniIbrahim, and Mercatali, Laura

Subjects
SQUAMOUS cell carcinoma, HEAD & neck cancer, PAPILLOMAVIRUSES, TREATMENT effectiveness, CELL lines, HYPOXIA-inducible factors
Abstract

Objective: Squamous cell carcinoma (SCC) represents the most common histotype of all head and neck malignancies and includes oropharyngeal squamous cell carcinoma (OSCC), a tumor associated with different clinical outcomes and linked to human papilloma virus (HPV) status. Translational research has few available in vitro models with which to study the different pathophysiological behavior of OSCCs. The present study proposes a 3-dimensional (3D) biomimetic collagen-based scaffold to mimic the tumor microenvironment and the crosstalk between the extracellular matrix (ECM) and cancer cells.Methods: We compared the phenotypic and genetic features of HPV-positive and HPV-negative OSCC cell lines cultured on common monolayer supports and on scaffolds. We also explored cancer cell adaptation to the 3D microenvironment and its impact on the efficacy of drugs tested on cell lines and primary cultures.Results: HPV-positive and HPV-negative cell lines were successfully grown in the 3D model and displayed different collagen fiber organization. The 3D cultures induced an increased expression of markers related to epithelial–mesenchymal transition (EMT) and to matrix interactions and showed different migration behavior, as confirmed by zebrafish embryo xenografts. The expression of hypoxia-inducible factor 1α (1α) and glycolysis markers were indicative of the development of a hypoxic microenvironment inside the scaffold area. Furthermore, the 3D cultures activated drug-resistance signaling pathways in both cell lines and primary cultures.Conclusions: Our results suggest that collagen-based scaffolds could be a suitable model for the reproduction of the pathophysiological features of OSCCs. Moreover, 3D architecture appears capable of inducing drug-resistance processes that can be studied to better our understanding of the different clinical outcomes of HPV-positive and HPV-negative patients with OSCCs. [ABSTRACT FROM AUTHOR]

Published
2021
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26. Metastatic neuroendocrine neoplasia treatments in patients over 70 years of age: A retrospective outcome analysis.

Author

Bongiovanni, Alberto, primary, Recine, Federica, additional, Foca, Flavia, additional, Fausti, Valentina, additional, Riva, Nada, additional, Calpona, Sebastiano, additional, Severi, Stefano, additional, Liverani, Chiara, additional, De Vita, Alessandro, additional, Spadazzi, Chiara, additional, Miserocchi, Giacomo, additional, Fabbri, Greta, additional, Mercatali, Laura, additional, Amadori, Dino, additional, and Ibrahim, Toni, additional

Published
2018
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27. Prognostic role of inflammatory index in head and neck squamous-cell carcinoma patients in first line chemotherapy.

Author

Fausti, Valentina, primary, Recine, Federica, additional, Foca, Flavia, additional, Calpona, Sebastiano, additional, Bruschi, Davide, additional, Bongiovanni, Alberto, additional, Riva, Nada, additional, Mercatali, Laura, additional, Miserocchi, Giacomo, additional, Liverani, Chiara, additional, De Vita, Alessandro, additional, Spadazzi, Chiara, additional, Di Iorio, Valentina, additional, Fabbri, Greta, additional, Dall'Agata, Monia, additional, Zavoiu, Venetia, additional, Martinelli, Giovanni, additional, Amadori, Dino, additional, and Ibrahim, Toni, additional

Published
2018
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28. Bone health management in early breast cancer patients: An Italian osteoncology center experience.

Author

Recine, Federica, primary, Bongiovanni, Alberto, additional, Fausti, Valentina, additional, Mercatali, Laura, additional, Riva, Nada, additional, Calpona, Sebastiano, additional, Faedi, Marina, additional, De Vita, Alessandro, additional, Liverani, Chiara, additional, Spadazzi, Chiara, additional, Miserocchi, Giacomo, additional, Foca, Flavia, additional, Vespignani, Roberto, additional, Rocca, Andrea, additional, Amadori, Dino, additional, and Ibrahim, Toni, additional

Published
2017
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29. Metronomic capecitabine in gastroenteropancreatic neuroendrocrine tumors: a suitable regimen and review of the literature

Author

Bongiovanni,Alberto, Riva,Nada, Calpona,Sebastiano, Ricci,Marianna, Gunelli,Erica, Liverani,Chiara, La Manna,Federico, De Vita,Alessandro, Monti,Manuela, Severi,Stefano, Pieri,Federica, Amadori,Elena, Galassi,Riccardo, Cavaliere,Davide, Zaccaroni,Alberto, Tartaglia,Andreas, Lunedei,Veronica, Gardini,Andrea, Mercatali,Laura, Amadori,Dino, Ibrahim,Toni, Bongiovanni,Alberto, Riva,Nada, Calpona,Sebastiano, Ricci,Marianna, Gunelli,Erica, Liverani,Chiara, La Manna,Federico, De Vita,Alessandro, Monti,Manuela, Severi,Stefano, Pieri,Federica, Amadori,Elena, Galassi,Riccardo, Cavaliere,Davide, Zaccaroni,Alberto, Tartaglia,Andreas, Lunedei,Veronica, Gardini,Andrea, Mercatali,Laura, Amadori,Dino, and Ibrahim,Toni

Abstract

Alberto Bongiovanni,1 Nada Riva,1 Sebastiano Calpona,1 Marianna Ricci,1 Erica Gunelli,1 Chiara Liverani,1 Federico La Manna,1 Alessandro De Vita,1 Manuela Monti,1 Stefano Severi,2 Federica Pieri,3 Elena Amadori,1 Riccardo Galassi,1 Davide Cavaliere,4 Alberto Zaccaroni,5 Andreas Tartaglia,6 Veronica Lunedei,7 Andrea Gardini,8 Laura Mercatali,1 Dino Amadori,1 Toni Ibrahim11Osteoncology and Rare Tumors Center, 2Nuclear Medicine Unit, Istituto Scientifico Romagnolo per lo Studio e la Cura dei Tumori (IRST) IRCCS, Meldola, 3Pathology Unit, 4Unit of Oncological Surgery and Advanced Therapies, 5Endocrine Surgery Unit, 6Endocrinology Unit, 7Gastroenterology and Digestive Endoscopy Unit, 8Department of General Surgery, Morgagni-Pierantoni Hospital, Forlì, ItalyBackground: We present a retrospective analysis of metronomic capecitabine in metastatic gastroenteropancreatic neuroendrocrine tumors (GEP-NETs). A review of the literature is also presented.Methods: From January 2007 to December 2013, ten patients with metastatic GEP-NETs (four pancreatic and six ileal) who progressed after treatment with somatostatin analogs and other cytotoxic agents received oral capecitabine 1,500 mg/day continuously. The median patient age was 68 (range 29–82) years. The median treatment duration was 8 months.Results: Five (50%) patients achieved a partial radiographic response, four (40%) showed stable disease, and one (10%) progressed. Median overall survival was 56 months. Three of the four pancreatic patients achieved a partial radiographic response that lasted for a median of 15.5 months; overall survival and progression-free survival in this subgroup was 58 and 6 months, respectively.Conclusion: Data in the literature show that capecitabine has only occasionally been used as a single agent, with increased toxicity. Only one study using single-agent capecitabine reported a progression-free survival of 9.9 months and overall survival of 36.5 months, without an objective

Published
2014

30. Pleural effusion in a patient with metastatic gastrointestinal stromal tumor treated with imatinib: case report

Author

Bongiovanni, Alberto, primary, Ricci, Marianna, additional, Riva, Nada, additional, Calpona, Sebastiano, additional, Oboldi, Devil, additional, Pieri, Federica, additional, Cavaliere, Davide, additional, Mercatali, Laura, additional, Liverani, Chiara, additional, La Manna, Federico, additional, De Vita, Alessandro, additional, Foca, Flavia, additional, Gunelli, Erica, additional, Amadori, Dino, additional, and Ibrahim, Toni, additional

Published
2014
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31. Metronomic capecitabine in gastroenteropancreatic neuroendrocrine tumors: a suitable regimen and review of the literature

Author

Ibrahim, Toni, primary, Bongiovanni, Alberto, additional, Riva, Nada, additional, Ricci, Marianna, additional, Calpona, Sebastiano, additional, Gunelli, Erica, additional, Liverani, Chiara, additional, la Manna, Federico, additional, de Vita, Alessandro, additional, Monti, Manuela, additional, Severi, Stefano, additional, Pieri, Federica, additional, Amadori, Elena, additional, Galassi, Riccardo, additional, Cavaliere, Davide, additional, Zaccaroni, Alberto, additional, Tartaglia, Andreas, additional, Lunedei, Veronica, additional, Mercatali, Laura, additional, Amadori, Dino, additional, and Gardini, Andrea, additional

Published
2014
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32. Single bone metastasis from adenocarcinoma of the lacrimal gland: a case report

Author

Ricci, Marianna, primary, Amadori, Elena, additional, Chiesa, Fausto, additional, Bongiovanni, Alberto, additional, Liverani, Chiara, additional, Fabbri, Laura, additional, Falasconi, Maria Cristina, additional, Casadei, Roberto, additional, Oboldi, Devil, additional, Galassi, Riccardo, additional, Micheletti, Simona, additional, Severi, Stefano, additional, Serra, Luigi, additional, Pieri, Federica, additional, Calabrese, Luca, additional, Riva, Nada, additional, Calpona, Sebastiano, additional, Gunelli, Erica, additional, Mercatali, Laura, additional, Amadori, Dino, additional, and Ibrahim, Toni, additional

Published
2014
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33. RANKL circulating levels in the evaluation of bone response in breast cancer (BC) patients.

Author

Ibrahim, Toni, primary, Mercatali, Laura, additional, Ricci, Marianna, additional, Scarpi, Emanuela, additional, Ricci, Rossana, additional, Bongiovanni, Alberto, additional, Zavoiu, Venetia, additional, Riva, Nada, additional, Calpona, Sebastiano, additional, Liverani, Chiara, additional, Oboldi, Devil, additional, Amadori, Elena, additional, La Manna, Federico, additional, De Vita, Alessandro, additional, Foca, Flavia, additional, Gunelli, Erica, additional, Monti, Manuela, additional, Serra, Patrizia, additional, and Amadori, Dino, additional

Published
2014
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34. Trefoil factor 1 as a predictive factor of bone metastases in breast cancer.

Author

Mercatali, Laura, primary, Kang, Yibin, additional, Zanoni, Michele, additional, Liverani, Chiara, additional, Carretta, Elisa, additional, Ricci, Marianna, additional, Riva, Nada, additional, Calpona, Sebastiano, additional, Bongiovanni, Alberto, additional, Zoli, Wainer, additional, Amadori, Dino, additional, and Ibrahim, Toni, additional

Published
2013
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35. Hemangioblastoma of the Gastrointestinal Tract

Author

Casadei Gardini, Andrea, primary, Pieri, Federica, additional, Fusaroli, Pietro, additional, Oboldi, Devil, additional, Passardi, Alessandro, additional, Monti, Manlio, additional, Rosetti, Paola, additional, Calpona, Sebastiano, additional, Valgiusti, Martina, additional, Ragazzini, Angela, additional, Amadori, Dino, additional, and Frassineti, Giovanni Luca, additional

Published
2013
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36. Metronomic capecitabine in gastroenteropancreatic neuroendrocrine tumors: a suitable regimen and review of the literature.

Author

Bongiovanni, Alberto, Riva, Nada, Calpona, Sebastiano, Ricci, Marianna, Gunelli, Erica, Liverani, Chiara, La Manna, Federico, De Vita, Alessandro, Monti, Manuela, Severi, Stefano, Pieri, Federica, Amadori, Elena, Galassi, Riccardo, Cavaliere, Davide, Zaccaroni, Alberto, Tartaglia, Andreas, Lunedei, Veronica, Gardini, Andrea, Mercatali, Laura, and Amadori, Dino

Subjects
SOMATOSTATIN, ANTINEOPLASTIC agents, DRUG toxicity, PROGRESSION-free survival, CANCER chemotherapy
Abstract

Background: We present a retrospective analysis of metronomic capecitabine in metastatic gastroenteropancreatic neuroendrocrine tumors (GEP-NETs). A review of the literature is also presented. Methods: From January 2007 to December 2013, ten patients with metastatic GEP-NETs (four pancreatic and six ileal) who progressed after treatment with somatostatin analogs and other cytotoxic agents received oral capecitabine 1,500 mg/day continuously. The median patient age was 68 (range 29-82) years. The median treatment duration was 8 months. Results: Five (50%) patients achieved a partial radiographic response, four (40%) showed stable disease, and one (10%) progressed. Median overall survival was 56 months. Three of the four pancreatic patients achieved a partial radiographic response that lasted for a median of 15.5 months; overall survival and progression-free survival in this subgroup was 58 and 6 months, respectively. Conclusion: Data in the literature show that capecitabine has only occasionally been used as a single agent, with increased toxicity. Only one study using single-agent capecitabine reported a progression-free survival of 9.9 months and overall survival of 36.5 months, without an objective response or major toxicity. In our experience, metronomic capecitabine was well tolerated, although minor side effects may have been underestimated due to the retrospective nature of our study. This regimen also seems to be feasible in elderly people. Although high response rates and prolonged response duration indicate the potential efficacy of this treatment, our results should be interpreted cautiously because of the small number of patients involved. Capecitabine was most effective in the pancreatic subgroup, which would seem to be more sensitive to chemotherapy. [ABSTRACT FROM AUTHOR]

Published
2014
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39. Abstract 333: Development of a biomimetic 3D scaffold to study breast cancer bone metastasis

Author

Laura Mercatali, Chiara Spadazzi, Federica Recine, Giacomo Miserocchi, Nicola Baldini, Silvia Angela De Bonis, Sebastiano Calpona, Toni Ibrahim, Giandomenico Di Menna, Alessandro De Vita, Nada Riva, Lorena Gurrieri, Chiara Liverani, Alberto Bongiovanni, Valentina Fausti, Giovanni Martinelli, Claudia Cocchi, Spadazzi, Chiara, Mercatali, Laura, De Vita, Alessandro, Liverani, Chiara, Cocchi, Claudia, Miserocchi, Giacomo, Bongiovanni, Alberto, Recine, Federica, Calpona, Sebastiano, Riva, Nada, Menna, Giandomenico Di, Gurrieri, Lorena, Fausti, Valentina, De Bonis, Silvia Angela, Martinelli, Giovanni, Baldini, Nicola, and Ibrahim, Toni

Subjects
Cancer Research, Tumor microenvironment, MMP2, biology, Chemistry, Bone metastasis, Osteoblast, Cell morphology, medicine.disease, 3D models, tumor microenvironment, bone metastasis, breast cancer, Extracellular matrix, medicine.anatomical_structure, Oncology, RANKL, Osteoclast, biology.protein, Cancer research, medicine
Abstract

Cancer metastasis is the leading cause of morbidity and cancer-related death. Bone is one of the most favored sites of metastatic spread, particularly from breast cancer. However, our understanding on how the host tissue can impact tumor growth is weak. This is hampered by the lack of a reliable in vitro model ables to replicate the complex microenvironment. Indeed, bone microenvironment provides physical support to cells but it can also affect cell behaviour and phenotype. We developed a hybrid 3D collagen-based scaffold functionalized with hydroxyapatite material (HA) in order to mimic natural bone features. Scaffolds were synthesized in our laboratory through collagen solution mixed and cross-linked with hydroxyapatite. Then, we cultured MDA-MB-231, a triple negative breast cancer cell line and MCF-7, an ER+ breast cancer cell line in collagen or hybrid scaffold, in order to evaluate how extracellular matrix affects cell behavior. Cell growth was evaluated by MTT cell-proliferation assay in a time course analysis. Moreover, we evaluated cell morphology and cell disposition within the scaffold by confocal microscopy. Finally, gene profiling was evaluated by qRT-PCR. MCF-7 cells acquire an organized and linear structure, whereas MDA-MB-231 an epithelial-like morphology, when cultured in hybrid scaffold. MDA-MB-231 cells proliferate faster than MCF-7 in both models. However, this is not affected by hydroxyapatite since the proliferation rate is similar when cells are cultured in collagen or hybrid scaffold. MDA-MB-231 grown in hybrid scaffold show a significant increase in RANKL/OPG ratio, pathway strictly involved in bone homeostasis. Moreover, they show a significant increased expression in JAG1, MMP2 and SNAIL1. For MCF-7, we observe a decrease in CDH1/VIM expression ratio and a significant decrease in OPG, osteoclastogenesis inhibitory factor. Overall, these results suggest a shift induced by hydroxyapatite scaffold towards an osteolytic phenotype. Whereas collagen-scaffold could better simulate in vivo primary tumor (Liverani C. et al, Sci Rep 9, 12263, 2019), we highlight that hybrid hydroxyapatite/collagen scaffold is more suitable to study breast cancer cells behaviour in bone metastasis. Then, we will implement it with a direct co-culture of osteoclast and osteoblast cells, in order to better investigate the contribution of the host microenvironment in bone metastasis. This model could provide a reliable 3D in vitro model for the study of bone metastasis mechanisms and for drug screening assays. Citation Format: Chiara Spadazzi, Laura Mercatali, Alessandro De Vita, Chiara Liverani, Claudia Cocchi, Giacomo Miserocchi, Alberto Bongiovanni, Federica Recine, Sebastiano Calpona, Nada Riva, Giandomenico Di Menna, Lorena Gurrieri, Valentina Fausti, Silvia Angela De Bonis, Giovanni Martinelli, Nicola Baldini, Toni Ibrahim. Development of a biomimetic 3D scaffold to study breast cancer bone metastasis [abstract]. In: Proceedings of the Annual Meeting of the American Association for Cancer Research 2020; 2020 Apr 27-28 and Jun 22-24. Philadelphia (PA): AACR; Cancer Res 2020;80(16 Suppl):Abstract nr 333.

Published
2020

40. Hemangioblastoma of the gastrointestinal tract: A first case

Author

Angela Ragazzini, Paola Rosetti, Dino Amadori, Pietro Fusaroli, Devil Oboldi, Giovanni Luca Frassineti, Andrea Casadei Gardini, Manlio Monti, Federica Pieri, Martina Valgiusti, Sebastiano Calpona, Alessandro Passardi, A. Casadei Gardini, F. Pieri, P. Fusaroli, D. Oboldi, A. Passardi, M. Monti, P. Rosetti, S. Calpona, M. Valgiusti, A. Ragazzini, D. Amadori, G. L. Frassineti, Casadei Gardini, Andrea, Pieri, Federica, Fusaroli, Pietro, Oboldi, Devil, Passardi, Alessandro, Monti, Manlio, Rosetti, Paola, Calpona, Sebastiano, Valgiusti, Martina, Ragazzini, Angela, Amadori, Dino, and Frassineti, Giovanni Luca

Subjects
Endoscopic ultrasound, Von Hippel-Lindau disease, Pathology, medicine.medical_specialty, Lower gastrointestinal bleeding, hemangioblastoma, Colorectal cancer, Colonoscopy, Breast Neoplasms, Pathology and Forensic Medicine, Lesion, ENDOSCOPIC ULTRASONOGRAPHY, Hemangioblastoma, Biomarkers, Tumor, Medicine, Humans, colon cancer, Anatomy, Surgery, 2734, Aged, Gastrointestinal Neoplasms, medicine.diagnostic_test, business.industry, Sigmoid colon, Cancer, Neoplasms, Second Primary, medicine.disease, Immunohistochemistry, medicine.anatomical_structure, Female, medicine.symptom, business
Abstract

We present the first documented case of hemangioblastoma located in the left colon. A 75-year-old woman undergoing adjuvant chemotherapy for breast cancer experienced rectal bleeding. Colonoscopy revealed a roundish mass covered with normal mucosa in the sigmoid colon. Endoscopic ultrasound showed an isoechoic lesion originating from the third layer of the intestinal wall; underlying layers were normal. Endoscopic ultrasound features were not suggestive of either cancer or malignant stromal tumor. Left hemicolectomy was subsequently performed due to repeated episodes of lower gastrointestinal bleeding. Grossly, a circumscribed submucosal yellowish nodule (13 mm) was observed, which was not attached to any peripheral nerve. Histologically, the lesion was composed of large, atypical cells traversed by a network of blood vessels. Immunohistochemically, the cells showed positivity for inhibin and NSE and weak positivity for S-100. A diagnosis of hemangioblastoma was made. This case highlights that hemangioblastoma of the gastrointestinal tract can also occur. © The Author(s) 2013.

Published
2013

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