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1. Comparing Long-Acting Antipsychotic Discontinuation Rates Under Ordinary Clinical Circumstances: A Survival Analysis from an Observational, Pragmatic Study

Author: Bertolini, F., Ostuzzi, G., Pievani, M., Aguglia, A., Bartoli, F., Bortolaso, P., Callegari, C., Caroleo, M., Carra, G., Corbo, M., D'Agostino, A., De Fazio, P., Magliocco, F., Martinotti, G., Ostinelli, E. G., Piccinelli, M. P., Tedeschi, F., Barbui, C., Boschello, F., Gastaldon, C., Mazzi, M. A., Nose, M., Papola, D., Perini, G., Piccoli, A., Purgato, M., Ruggeri, M., Terlizzi, S., Turrini, G., Raffaele, G., Cavallotti, S., Chirico, M., Ferrato, F., Limosani, I., Mastromo, D., Monzani, E., Porcellana, M., Restaino, F., Annese, P. M., Bolognesi, S., Cerretini, M., De Capua, A., Debolini, S., Del Zanna, M., Fargnoli, F., Giannini, A., Luccarelli, L., Lucii, C., Pierantozzi, E., Tozzi, F., Bardicchia, F., Cardamone, G., Facchi, E., Magnani, N., Soscia, F., Biancosino, B., Zotos, S., Giacomin, M., Pompei, F., Spano, M., Zonta, F., Buzzi, A., Callegred, C., Calzolari, R., Caselli, I., Diurni, M., Giana, E., Ielmini, M., Milano, A., Poloni, N., Sani, E., Zizolfi, D., Alberini, G., Cazzamalli, S., Costantini, C., Di Caro, A., Paronelli, C., Piantanida, S., Piccinelli, M., Alessandro, P., Barbanti, S. V., D'Ippolito, C., Gozzi, M., Moretti, V., Campese, O., Di Capro, L., di Giannantonio, M., Fiori, F., Lorusso, M., Mancini, V., Viceconte, D., Calandra, C., Luca, M., Signorelli, M. S., Suraniti, F., Balzarro, B., Boncompagni, G., Caretto, V., Emiliani, R., Lupoli, P., Menchetti, M., Rossi, E., Storbini, V., Tarricone, I., Terzi, L., Boso, M., Catania, C., De Paoli, G., Risaro, P., Aspesi, F., Bava, M., Bono, A., Brambilla, G., Castagna, G., Lucchi, S., Nava, R., Provenzi, M., Tabacchi, T., Tremolada, M., Verrengia, E., Barchiesi, M., Oriani, M. G., Pacetti, M., Ferro, M., Ghio, L., Beneduce, R., Laffranchini, L., Magni, L. R., Rossi, G., Tura, G. B., Addeo, L., Balletta, G., De Vivo, E., Di Benedetto, R., Parise, V. F., Carpiniello, B., Pinna, F., Pecile, D., Mattei, C., Bonavigo, T., Fabrici, E. P., Panarello, S., Peresson, G., Vitucci, C., Gardellin, F., Strizzolo, S., Cossetta, E., Fizzotti, C., Moretti, D., Di Gregorio, L., Sozzi, F., Colli, G., La Barbera, D., Laurenzi, S., Bertolini, F, Ostuzzi, G, Pievani, M, Aguglia, A, Bartoli, F, Bortolaso, P, Callegari, C, Caroleo, M, Carra, G, Corbo, M, D'Agostino, A, De Fazio, P, Magliocco, F, Martinotti, G, Ostinelli, E, Piccinelli, M, Tedeschi, F, Barbui, C, Boschello, F, Gastaldon, C, Mazzi, M, Nose, M, Papola, D, Perini, G, Piccoli, A, Purgato, M, Ruggeri, M, Terlizzi, S, Turrini, G, Raffaele, G, Cavallotti, S, Chirico, M, Ferrato, F, Limosani, I, Mastromo, D, Monzani, E, Porcellana, M, Restaino, F, Annese, P, Bolognesi, S, Cerretini, M, De Capua, A, Debolini, S, Del Zanna, M, Fargnoli, F, Giannini, A, Luccarelli, L, Lucii, C, Pierantozzi, E, Tozzi, F, Bardicchia, F, Cardamone, G, Facchi, E, Magnani, N, Soscia, F, Biancosino, B, Zotos, S, Giacomin, M, Pompei, F, Spano, M, Zonta, F, Buzzi, A, Calzolari, R, Caselli, I, Diurni, M, Giana, E, Ielmini, M, Milano, A, Poloni, N, Sani, E, Zizolfi, D, Alberini, G, Cazzamalli, S, Costantini, C, Di Caro, A, Paronelli, C, Piantanida, S, Alessandro, P, Barbanti, S, D'Ippolito, C, Gozzi, M, Moretti, V, Campese, O, Di Capro, L, di Giannantonio, M, Fiori, F, Lorusso, M, Mancini, V, Viceconte, D, Calandra, C, Luca, M, Signorelli, M, Suraniti, F, Balzarro, B, Boncompagni, G, Caretto, V, Emiliani, R, Lupoli, P, Menchetti, M, Rossi, E, Storbini, V, Tarricone, I, Terzi, L, Boso, M, Catania, C, De Paoli, G, Risaro, P, Aspesi, F, Bava, M, Bono, A, Brambilla, G, Castagna, G, Lucchi, S, Nava, R, Provenzi, M, Tabacchi, T, Tremolada, M, Verrengia, E, Barchiesi, M, Oriani, M, Pacetti, M, Ferro, M, Ghio, L, Beneduce, R, Laffranchini, L, Magni, L, Rossi, G, Tura, G, Addeo, L, Balletta, G, De Vivo, E, Di Benedetto, R, Parise, V, Carpiniello, B, Pinna, F, Pecile, D, Mattei, C, Bonavigo, T, Fabrici, E, Panarello, S, Peresson, G, Vitucci, C, Gardellin, F, Strizzolo, S, Cossetta, E, Fizzotti, C, Moretti, D, Di Gregorio, L, Sozzi, F, Colli, G, La Barbera, D, Laurenzi, S, Bertolini F., Ostuzzi G., Pievani M., Aguglia A., Bartoli F., Bortolaso P., Callegari C., Caroleo M., Carra G., Corbo M., D'Agostino A., De Fazio P., Magliocco F., Martinotti G., Ostinelli E.G., Piccinelli M.P., Tedeschi F., Barbui C., Boschello F., Gastaldon C., Mazzi M.A., Nose M., Papola D., Perini G., Piccoli A., Purgato M., Ruggeri M., Terlizzi S., Turrini G., Raffaele G., Cavallotti S., Chirico M., Ferrato F., Limosani I., Mastromo D., Monzani E., Porcellana M., Restaino F., Annese P.M., Bolognesi S., Cerretini M., De Capua A., Debolini S., Del Zanna M., Fargnoli F., Giannini A., Luccarelli L., Lucii C., Pierantozzi E., Tozzi F., Bardicchia F., Cardamone G., Facchi E., Magnani N., Soscia F., Biancosino B., Zotos S., Giacomin M., Pompei F., Spano M., Zonta F., Buzzi A., Callegred C., Calzolari R., Caselli I., Diurni M., Giana E., Ielmini M., Milano A., Poloni N., Sani E., Zizolfi D., Alberini G., Cazzamalli S., Costantini C., Di Caro A., Paronelli C., Piantanida S., Piccinelli M., Alessandro P., Barbanti S.V., D'Ippolito C., Gozzi M., Moretti V., Campese O., Di Capro L., di Giannantonio M., Fiori F., Lorusso M., Mancini V., Viceconte D., Calandra C., Luca M., Signorelli M.S., Suraniti F., Balzarro B., Boncompagni G., Caretto V., Emiliani R., Lupoli P., Menchetti M., Rossi E., Storbini V., Tarricone I., Terzi L., Boso M., Catania C., De Paoli G., Risaro P., Aspesi F., Bava M., Bono A., Brambilla G., Castagna G., Lucchi S., Nava R., Provenzi M., Tabacchi T., Tremolada M., Verrengia E., Barchiesi M., Oriani M.G., Pacetti M., Ferro M., Ghio L., Beneduce R., Laffranchini L., Magni L.R., Rossi G., Tura G.B., Addeo L., Balletta G., De Vivo E., Di Benedetto R., Parise V.F., Carpiniello B., Pinna F., Pecile D., Mattei C., Bonavigo T., Fabrici E.P., Panarello S., Peresson G., Vitucci C., Gardellin F., Strizzolo S., Cossetta E., Fizzotti C., Moretti D., Di Gregorio L., Sozzi F., Colli G., La Barbera D., and Laurenzi S.
Subjects: Male, Pediatrics, respectively), 0302 clinical medicine, Delayed-Action Preparation, Brief Psychiatric Rating Scale, Pharmacology (medical), he STAR Network ‘Depot Study’ prospectively followed 394 subjects initiating treatment with long-acting injections (LAIs) of antipsychotics under naturalistic conditions for 12 months. LAI discontinuation was frequent in everyday clinical practice in Italy, Original Research Article, Prospective Studies, Prospective cohort study, treatment, Mental Disorders, Hazard ratio, whereas more than half of participants initiating risperidone LAI and olanzapine LAI discontinued during the 12 months of follow-up (51.4 and 62.5%, Psychiatric Status Rating Scale, Middle Aged, side efects, Psychiatry and Mental health, Italy, Mental Disorder, Female, he STAR Network ‘Depot Study’ prospectively followed 394 subjects initiating treatment with long-acting injections (LAIs) of antipsychotics under naturalistic conditions for 12 months. LAI discontinuation was frequent in everyday clinical practice in Italy, occurring in almost 40% of the entire sample, side efects, participant refusal to continue LAIs and LAIs no longer being required were the most frequently reported reasons for discontinuation. Paliperidone LAI and aripiprazole LAI were the least discontinued medications (33.9 and 35.4%, respectively), whereas more than half of participants initiating risperidone LAI and olanzapine LAI discontinued during the 12 months of follow-up (51.4 and 62.5%, respectively). In multivariate analysis, being prescribed olanzapine LAI and poor medication adherence at baseline were signifcantly associated with higher discontinuation risk, Human, Antipsychotic Agents, medicine.drug, Psychopathology, Adult, medicine.medical_specialty, Discontinuation, Follow-Up Studie, Medication Adherence, 03 medical and health sciences, medicine, Humans, Paliperidone, Adverse effect, Settore MED/25 - Psichiatria, discontinuation rates, Psychiatric Status Rating Scales, respectively). In multivariate analysis, business.industry, Long-Acting Antipsychotic, long-acting injectable antipsychotics, Survival Analysis, Confidence interval, participant refusal to continue LAIs and LAIs no longer being required were the most frequently reported reasons for discontinuation. Paliperidone LAI and aripiprazole LAI were the least discontinued medications (33.9 and 35.4%, 030227 psychiatry, Prospective Studie, Antipsychotic Agent, occurring in almost 40% of the entire sample, Delayed-Action Preparations, Neurology (clinical), business, 030217 neurology & neurosurgery, being prescribed olanzapine LAI and poor medication adherence at baseline were signifcantly associated with higher discontinuation risk, Follow-Up Studies
Abstract: Background Recent guidelines suggested a wider use of long-acting injectable antipsychotics (LAI) than previously, but naturalistic data on the consequences of LAI use in terms of discontinuation rates and associated factors are still sparse, making it hard for clinicians to be informed on plausible treatment courses. Objective Our objective was to assess, under real-world clinical circumstances, LAI discontinuation rates over a period of 12 months after a first prescription, reasons for discontinuation, and associated factors. Methods The STAR Network ‘Depot Study’ was a naturalistic, multicentre, observational prospective study that enrolled subjects initiating a LAI without restrictions on diagnosis, clinical severity or setting. Participants from 32 Italian centres were assessed at baseline and at 6 and 12 months of follow-up. Psychopathology, drug attitude and treatment adherence were measured using the Brief Psychiatric Rating Scale, the Drug Attitude Inventory and the Kemp scale, respectively. Results The study followed 394 participants for 12 months. The overall discontinuation rate at 12 months was 39.3% (95% confidence interval [CI] 34.4–44.3), with paliperidone LAI being the least discontinued LAI (33.9%; 95% CI 25.3–43.5) and olanzapine LAI the most discontinued (62.5%; 95% CI 35.4–84.8). The most frequent reason for discontinuation was onset of adverse events (32.9%; 95% CI 25.6–40.9) followed by participant refusal of the medication (20.6%; 95% CI 14.6–27.9). Medication adherence at baseline was negatively associated with discontinuation risk (hazard ratio [HR] 0.853; 95% CI 0.742–0.981; p = 0.026), whereas being prescribed olanzapine LAI was associated with increased discontinuation risk compared with being prescribed paliperidone LAI (HR 2.156; 95% CI 1.003–4.634; p = 0.049). Conclusions Clinicians should be aware that LAI discontinuation is a frequent occurrence. LAI choice should be carefully discussed with the patient, taking into account individual characteristics and possible obstacles related to the practicalities of each formulation. Supplementary Information The online version contains supplementary material available at 10.1007/s40263-021-00809-w.
Published: 2021

2. How to COVID-19 affected sleep talking episodes?

Author: Camaioni, M., Scarpelli, S., Alfonsi, V., Gorgoni, M., Calzolari, R., Bartolo, M., Mangiaruga, A., and Luigi De Gennaro
Subjects: parasomnia, sleep talking, dreaming
Published: 2022

3. Clinical correlates of paliperidone palmitate and aripiprazole monohydrate prescription for subjects with schizophreniaspectrum disorders: Findings from the STAR Network Depot Study

Author: Barbui C, Nosè M, Purgato M, Turrini G, Ostuzzi G, Mazzi MA, Papola D, Gastaldon C, Terlizzi S, Bertolini F, Piccoli A, Ruggeri M, De Fazio P, Magliocco F, Caroleo M, Raffaele G, D'Agostino A, Ostinelli EG, Chirico M, Cavallotti S, Lucii C, Bolognesi S, Debolini S, Pierantozzi E, Fargnoli F, Del Zanna M, Giannini A, Luccarelli L, De Capua A, Annese PM, Cerretini M, Tozzi F, Magnani N, Cardamone G, Bardicchia F, Facchi E, Soscia F, Zotos S, Biancosino B, Zonta F, Pompei F, Callegari C, Zizolfi D, Poloni N, Ielmini M, Caselli I, Giana E, Buzzi A, Diurni M, Milano A, Sani E, Calzolari R, Bortolaso P, Piccinelli M, Cazzamalli S, Alberini G, Piantanida S, Costantini C, Paronelli C, Di Caro A, Moretti V, Gozzi M, D'Ippolito C, Barbanti SV, Papalini A, Corbo M, Martinotti G, Campese O, Fiori F, Lorusso M, Di Capro L, Viceconte D, Mancini V, Suraniti F, Signorelli MSI, Rossi E, Lupoli P, Menchetti M, Terzi L, Boso M, Risaro P, De Paoli G, Catania C, Tarricone I, Caretto V, Storbini V, Emiliani R, Balzarro B, Carrà G, Bartoli F, Tabacchi T, Nava R, Bono A, Provenzi M, Brambilla G, Aspesi F, Trotta G, Tremolada M, Castagna G, Bava M, Verrengia E, Lucchi S, Oriani MG, Barchiesi M, Pacetti M, Aguglia A, Magni LR, Rossi G, Beneduce R, Tura GB, Laffranchini L, Mastromo D, Ferrato F, Restaino F, Monzani E, Porcellana M, Limosani I, Ghio L, Ferro M, Parise VF, Balletta G, Addeo L, De Vivo E, Di Benedetto R, Pinna F, Carpiniello B, Spano M, Giacomin M, Pecile D, Mattei C, Fabrici EP, Panarello S, Peresson G, Vitucci C, Bonavigo T, Perini G, Boschello F, Strizzolo S, Gardellin F, Di Giannantonio M, Moretti D, Fizzotti C, Cossetta E, Di Gregorio L, Sozzi F, Boncompagni G, La Barbera D, Colli G, Laurenzi S, Calandra C, Luca M, Crocamo C, STAR Network Depot Investigators, Bartoli F., Ostuzzi G., Crocamo C., Corbo M., D'Agostino A., Martinotti G., Ostinelli E.G., Tabacchi T., Barbui C., Carr G., Nose M., Purgato M., Turrini G., Mazzi M.A., Papola D., Gastaldon C., Terlizzi S., Bertolini F., Piccoli A., Ruggeri M., De Fazio P., Magliocco F., Caroleo M., Raffaele G., Chirico M., Cavallotti S., Lucii C., Bolognesi S., Debolini S., Pierantozzi E., Fargnoli F., Del Zanna M., Giannini A., Luccarelli L., De Capua A., Annese P.M., Cerretini M., Tozzi F., Magnani N., Cardamone G., Bardicchia F., Facchi E., Soscia F., Zotos S., Biancosino B., Zonta F., Pompei F., Callegari C., Zizolfi D., Poloni N., Ielmini M., Caselli I., Giana E., Buzzi A., Diurni M., Milano A., Sani E., Calzolari R., Bortolaso P., Piccinelli M., Cazzamalli S., Alberini G., Piantanida S., Costantini C., Paronelli C., Di Caro A., Moretti V., Gozzi M., D'Ippolito C., Barbanti S.V., Papalini A., Campese O., Fiori F., Lorusso M., Di Capro L., Viceconte D., Mancini V., Suraniti F., Signorelli M.S., Rossi E., Lupoli P., Menchetti M., Terzi L., Boso M., Risaro P., De Paoli G., Catania C., Tarricone I., Caretto V., Storbini V., Emiliani R., Balzarro B., Carra G., Nava R., Bono A., Provenzi M., Brambilla G., Aspesi F., Trotta G., Tremolada M., Castagna G., Bava M., Verrengia E., Lucchi S., Oriani M.G., Barchiesi M., Pacetti M., Aguglia A., Magni L.R., Rossi G., Beneduce R., Tura G.B., Laffranchini L., Mastromo D., Ferrato F., Restaino F., Monzani E., Porcellana M., Limosani I., Ghio L., Ferro M., Parise V.F., Balletta G., Addeo L., De Vivo E., Di Benedetto R., Pinna F., Carpiniello B., Spano M., Giacomin M., Pecile D., Mattei C., Fabrici E.P., Panarello S., Peresson G., Vitucci C., Bonavigo T., Perini G., Boschello F., Strizzolo S., Gardellin F., Di Giannantonio M., Moretti D., Fizzotti C., Cossetta E., Di Gregorio L., Sozzi F., Boncompagni G., La Barbera D., Colli G., Laurenzi S., Calandra C., Luca M., Barbui C, Nosè M, Purgato M, Turrini G, Ostuzzi G, Mazzi MA, Papola D, Gastaldon C, Terlizzi S, Bertolini F, Piccoli A, Ruggeri M, De Fazio P, Magliocco F, Caroleo M, Raffaele G, D'Agostino A, Ostinelli EG, Chirico M, Cavallotti S, Lucii C, Bolognesi S, Debolini S, Pierantozzi E, Fargnoli F, Del Zanna M, Giannini A, Luccarelli L, De Capua A, Annese PM, Cerretini M, Tozzi F, Magnani N, Cardamone G, Bardicchia F, Facchi E, Soscia F, Zotos S, Biancosino B, Zonta F, Pompei F, Callegari C, Zizolfi D, Poloni N, Ielmini M, Caselli I, Giana E, Buzzi A, Diurni M, Milano A, Sani E, Calzolari R, Bortolaso P, Piccinelli M, Cazzamalli S, Alberini G, Piantanida S, Costantini C, Paronelli C, Di Caro A, Moretti V, Gozzi M, D'Ippolito C, Barbanti SV, Papalini A, Corbo M, Martinotti G, Campese O, Fiori F, Lorusso M, Di Capro L, Viceconte D, Mancini V, Suraniti F, Signorelli MSI, Rossi E, Lupoli P, Menchetti M, Terzi L, Boso M, Risaro P, De Paoli G, Catania C, Tarricone I, Caretto V, Storbini V, Emiliani R, Balzarro B, Carrà G, Bartoli F, Tabacchi T, Nava R, Bono A, Provenzi M, Brambilla G, Aspesi F, Trotta G, Tremolada M, Castagna G, Bava M, Verrengia E, Lucchi S, Oriani MG, Barchiesi M, Pacetti M, Aguglia A, Magni LR, Rossi G, Beneduce R, Tura GB, Laffranchini L, Mastromo D, Ferrato F, Restaino F, Monzani E, Porcellana M, Limosani I, Ghio L, Ferro M, Parise VF, Balletta G, Addeo L, De Vivo E, Di Benedetto R, Pinna F, Carpiniello B, Spano M, Giacomin M, Pecile D, Mattei C, Fabrici EP, Panarello S, Peresson G, Vitucci C, Bonavigo T, Perini G, Boschello F, Strizzolo S, Gardellin F, Di Giannantonio M, Moretti D, Fizzotti C, Cossetta E, Di Gregorio L, Sozzi F, Boncompagni G, La Barbera D, Colli G, Laurenzi S, Calandra C, Luca M, Crocamo C, STAR Network Depot Investigators, Bartoli, F, Ostuzzi, G, Crocamo, C, Corbo, M, D'Agostino, A, Martinotti, G, Ostinelli, E, Tabacchi, T, Barbui, C, and Carra, G
Subjects: Aripiprazole monohydrate, Long-acting injectable antipsychotics, Paliperidone palmitate, Schizophrenia, Adult, Antipsychotic Agents, Aripiprazole, Female, Health Knowledge, Attitudes, Practice, Humans, Male, Medication Adherence, Paliperidone Palmitate, Practice Patterns, Physicians', Schizophrenic Psychology, Young Adult, Long-acting injectable antipsychotic, medicine.medical_specialty, medicine.medical_treatment, Aripiprazole monohydrate, Long-acting injectable antipsychotics, Paliperidone palmitate, Schizophrenia, Practice Patterns, 03 medical and health sciences, 0302 clinical medicine, Internal medicine, Brief Psychiatric Rating Scale, medicine, Pharmacology (medical), Antipsychotic, Settore MED/25 - Psichiatria, Practice, Physicians', business.industry, Health Knowledge, medicine.disease, 030227 psychiatry, Psychiatry and Mental health, Attitudes, Propensity score matching, Observational study, business, 030217 neurology & neurosurgery, medicine.drug, Psychopathology
Abstract: This study, based on the 'Servizi Territoriali Associati per la Ricerca' (STAR) Network Depot Study nationwide baseline data, explored whether individual symptoms severity and clusters might influence the prescription of paliperidone palmitate 1-month (PP1M) vs. aripiprazole monohydrate. The Brief Psychiatric Rating Scale (BPRS) was used to assess psychopathology and relevant symptoms clusters. Drug Attitude Inventory, 10 items, was used to test attitude towards medications. Adherence to treatments was rated according to the Kemp seven-point scale. We assessed for eligibility 451 individuals and, among them, we included 195 subjects (n = 117 who started PPM1 and n = 78 aripiprazole monohydrate). Individuals were comparable in terms of age, gender, treatment years, recent hospitalizations, previous long-acting injectable antipsychotic treatments, additional oral treatments, attitude toward drugs, medication adherence, and alcohol/substance-related comorbidities. Subjects starting PP1M presented higher BPRS overall (P = 0.009), positive (P = 0.015), and negative (P = 0.010) symptom scores compared to subjects starting aripiprazole monohydrate. Results were confirmed by appropriate regression models and propensity score matching analysis. No differences were found comparing the other BPRS subscale scores: affect, resistance, and activation. Clinicians may be more prone to prescribe PPM1, rather than aripiprazole monohydrate, to subjects showing higher overall symptom severity, including positive and negative symptoms. No additional clinical factors influenced prescribing attitudes in our sample.
Published: 2020

4. Impact of the environment on the health: From theory to practice

Author: Carducci, A.L., primary, Agodi, A., additional, Ancona, C., additional, Angelini, P., additional, Bagordo, F., additional, Barbone, F., additional, Birbaum, L., additional, Carreri, V., additional, Casuccio, A., additional, Conti, A., additional, Conversano, M., additional, De Donno, A., additional, De Giglio, O., additional, Desiante, F., additional, Di Pietro, A., additional, Dogliotti, E., additional, Donato, F., additional, Fara, G.M., additional, Fiore, M., additional, Forastiere, F., additional, Giammanco, G., additional, Izzotti, A., additional, Montagna, M.T., additional, Oliveri Conti, G., additional, Petronio, M.G., additional, Sciacca, S., additional, Signorelli, C., additional, Testai, E., additional, Verani, M., additional, Vinceti, M., additional, Vitale, F., additional, Ferrante, M., additional, Adani, G., additional, Berghella, L., additional, Calia, C., additional, Calzolari, R., additional, Canale, A., additional, Castiglione, D., additional, Copat, C., additional, Cristaldi, A., additional, Cuffari, G., additional, Coronel Vargas, G., additional, De Vita, E., additional, De Nard, F., additional, Federigi, I., additional, Filippini, T., additional, Grasso, A., additional, Leonardi, N., additional, Letzgus, M., additional, Lo Bianco, G., additional, Mazzucco, W., additional, Nicolosi, I., additional, Orlandi, P., additional, Paladino, G., additional, Pizzo, S., additional, Pousis, C., additional, Raffo, M., additional, Rivolta, S., additional, Scarpitta, F., additional, Trani, G., additional, Triggiano, F., additional, Tumbarello, A., additional, Vecchio, V., additional, Zuccarello, P., additional, and Vassallo, M., additional
Published: 2021
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5. Efficacy of Rapamycin as Inducer of Hb F in Primary Erythroid Cultures from Sickle Cell Disease and β-Thalassemia Patients

Author: Pecoraro, A, Troia, A, Calzolari, R, SCAZZONE, Concetta, Rigano, P, Martorana, A, Sacco, M, Maggio, A, Marzo RD, Pecoraro, A, Troia, A, Calzolari, R, Scazzone, C, Rigano, P, Martorana, A, Sacco, M, Maggio, A, and Marzo RD
Subjects: rapamycin, β-Thalassemia
Abstract: Phenotypic improvement of hemoglobinopathies such as sickle cell disease and β-thalassemia (β-thal) has been shown in patients with high levels of Hb F. Among the drugs proposed to increase Hb F production, hydroxyurea (HU) is currently the only one proven to improve the clinical course of these diseases. However, Hb F increase and patient's response are highly variable, indicating that new pharmacological agents could be useful for patients not responding to HU or showing a reduction of response during long-term therapy. In this study we evaluated the efficacy of rapamycin, a lypophilic macrolide used for the prevention of acute rejection in renal transplant recipients, as an inducer of Hb F production. The analyses were performed in cultured erythroid progenitors from 25 sickle cell disease and 25 β-thal intermedia (β-TI) patients. The use of a quantitative Real-Time-polymerase chain reaction ReTi-PCR technique and high performance liquid chromatography (HPLC) allowed us to determine the increase in γ-globin mRNA expression and Hb F production in human erythroid cells treated with rapamycin. The results of our study demonstrated an increase in vitro of γ-globin mRNA expression in 15 sickle cell disease and 14 β-TI patients and a corresponding Hb F increase. The induction by rapamycin, even if lower or similar in most of samples analyzed, in some cases was higher than HU. These data suggest that rapamycin could be a good candidate to be used in vivo for the treatment of hemoglobinopathies.
Published: 2015

6. Study on the efficacy of Rapamicin as an inducer of fetal hemoglobin in primary erythroid cultures from patientes with hemoglobinopathies

Author: SCAZZONE, Concetta, BONO, Antonino, Pecoraro,A, Troia,A, Calzolari, R, Di Marzo,R, Scazzone,C, Pecoraro,A, Troia,A, Calzolari, R, Di Marzo,R, and Bono, A
Subjects: Rapamicin, hemoglobinopathies
Published: 2011

7. Study on the efficacy of Rapamicin as an inducer of fetal hemoglobin in primary erythroid cultures from patientes with hemoglobinopathies

Author: Di Marzo, R, Pecoraro, A, Troia, A, Calzolari , R, Spina, V, Motta, V, Di Stefano, R, Maggio, A, SCAZZONE, Concetta, BONO, Antonino, Di Marzo, R, Pecoraro, A, Troia, A, Scazzone, C, Calzolari , R, Spina, V, Motta, V, Di Stefano, R, Maggio, A, and Bono, A
Subjects: Rapamicin, hemoglobinopathies
Published: 2009

8. Sea urchin sns chromatin insulator prebents silencing and positional effect variegation of oncoretroviral vectors transgene expression in murine erythroid cell line

Author: D'APOLITO, Danilo, MOTTA, Valentina, FRANCO, Vito, ACUTO S, DI MARZO R, BAGLIESI M, BAIAMONTE E, CALZOLARI R, SPINELLI G, MAGGIO A., D'APOLITO D, ACUTO S, DI MARZO R, BAGLIESI M, MOTTA V, BAIAMONTE E, CALZOLARI R, FRANCO V, SPINELLI G, and MAGGIO A
Published: 2006

9. The sea urchin chromatin insulator, sns-5 protect retrovirus vector from chromosomal position effects

Author: D'APOLITO, Danilo, FRANCO, Vito, MOTTA, Valentina, BAIAMONTE E, BAGLIESI M, PECORARO A, CALZOLARI R, BORRUSO V, SPINELLI G, MAGGIO A, ACUTO S., D'APOLITO D, BAIAMONTE E, BAGLIESI M, PECORARO A, CALZOLARI R, BORRUSO V, SPINELLI G, FRANCO V, MAGGIO A, MOTTA V, and ACUTO S
Published: 2006

10. Sea Urchin sns chromatin insulator prevents silencing and position effect variagation of oncoretroviral vectors transgene expression in murine cell line

Author: D'APOLITO, Danilo, MOTTA, Valentina, SPINELLI, Giovanni, ACUTO S, DI MARZO R, BAGLIESI M, BAIAMONTE E, CALZOLARI R, A, VITO F, MAGGIO A., D'APOLITO D, ACUTO S, DI MARZO R, BAGLIESI M, MOTTA V, BAIAMONTE E, CALZOLARI R, VITO F, SPINELLI G, and MAGGIO A
Published: 2006

11. Functional characterization and use in the retrovirus vectors of the sea urchin SNS chromatin insulator in erythroid cells

Author: ACUTO S, DI MARZO R, BAIAMONTE E, CALZOLARI R, BAGLIESI M, MAGGIO A., D'APOLITO, Danilo, MOTTA, Valentina, FRANCO, Vito, SPINELLI, Giovanni, ACUTO S, D'APOLITO D, DI MARZO R, BAIAMONTE E, CALZOLARI R, BAGLIESI M, MOTTA V, FRANCO V, SPINELLI G, and MAGGIO A
Published: 2005

12. Terapia genica delle emoglobinopatie:stato dell'arte

Author: ACUTO S, DI MARZO R, BAIAMONTE E, CALZOLARI R, BAGLIESI M, SPINBA B, TROIA F, VITO F, D'APOLITO, Danilo, MOTTA, Valentina, SPINELLI, Giovanni, ACUTO S, DI MARZO R, D'APOLITO D, BAIAMONTE E, CALZOLARI R, BAGLIESI M, MOTTA V, SPINBA B, TROIA F, VITO F, and SPINELLI G
Published: 2005

13. Analysis of Summer Ozone Observations at a High Mountain Site in Central Italy (Campo Imperatore, 2388 m a.s.l.)

Author: Cristofanelli, P., DI CARLO, Piero, Altorio, A. D. ’., DARI SALISBURGO, C., Biancofiore, P. TUCCELLA F., Stocchi, P., Verza, G. P., Landi, T. C., Marinoni, A., Duchi, F. CALZOLARI R., and Paolo, Bonasoni
Published: 2013

14. Environmental indicators in EpiAir2 project: Air quality data for epidemiological surveillance [Indicatori ambientali nello studio EpiAir2: I dati di qualità dell'aria per la sorveglianza epidemiologica]

Author: Gandini, M., Berti, G., Cattani, G., Faustini, A., Scarinzi, C., Donato, F., Accetta, G., Angiuli, L., Caldara, S., Carreras, G., Casale, P., Di Biagio, K., Giannini, S., Iuzzolino, C., Lanzani, G., Lauriola, P., Leuci, P., Mariuz, M., Marchesi, S., Nocioni, A., Pistollato, S., Pizzi, L., Ranzi, A., Serinelli, M., Stagnaro, E., Vianello, L., Vigotti, M., Zauli-Sajani, S., Cadum, E., Alessandrini, E. R., Angelini, P., Antonelli, A., Arena, P., Assennato, G., Baccini, M., Baldacchini, F., Baldacci, S., Baldini, M., Baldo, V., Ballarino, G., Balzi, D., Barban, I., Barbati, G., Barbone, F., Barchielli, A., Baroni, S., Barontini, R., Bartolacci, S., Bellodi, S., Berico, M., Biggeri, A., Bisanti, L., Bonfanti, M., Bonomolo, A., Bonvicini, L., Bovenzi, M., Brescianini, C., Broccoli, S., Bruni, A., Calzolari, R., Canossa, E., Canova, C., Caramia, G., Casale, G., Casella, C., Casetta, A., Cernigliaro, A., Cerrai, S., Cervino, M., Cestari, L., Chellini, E., Chiusolo, M., Ciaccia, G., Cibella, F., Cocchio, S., Crosetto, L., Cuccaro, F., Daris, F., Girolamo, G., Togni, A., Di Leo, A., Di Lonardo, S., Dessì, M. P., Evangelista, A., Faccini, F., Fallani, G., Forastiere, F., Fruzzetti, R., Galassi, C., Gallo, G., Gallo, L., Ganzi, A., Garrone, E., Gatti, G., Gherardi, B., Gianicolo, E. A. L., Giua, R., Goldoni, C. A., Grechi, D., Grosa, M., Guerrini, N., Impallomeni, M., La Torretta, T., Luberto, F., Lupi, A., Madonia, G., Maio, S., Malaguti, A., Mangia, C., Mariottini, M., Marotta, A., Martini, A., Menegotto, M., Merlo, F., Migliore, E., Minerba, S., Minichilli, F., Mircea, M., Morassuto, C., Nuvolone, D., Onorati, R. N. G., Padolecchia, G., Palazzi, B., Pandolfi, P., Parodi, S., Pasetti, P., Pensato, A., Piersanti, A., Piovesan, C., Pizzuti, R., Addario, S. P., Porcaro, L., Protti, M., Puppo, A., Raciti, M., Randi, G., Rosa, M., Rubini, G., Sacco, F., Santoro, M., Sarno, G., Scala, D., Scondotto, S., Selle, V., Simeoni, R., Simonato, L., Simoni, M., Spagnolo, G., Massimo Stafoggia, Starnino, G., Stivanello, E., Tominz, R., Troiano, F., Vercelli, M., Viegi, G., Vigotti, M. A., Volta, C., Zanini, G., and Zero, F.
Published: 2013

15. Inactivation of C3H10T1/2 cells by low energy protons and deuterons

Author: BETTEGA P. CALZOLARI R. MARCHESINI, D., primary
Published: 1998
Full Text: View/download PDF

16. Clinical and Hematological Response to Hydroxyurea in a Patient with Hb Leporbp-Thalassemia

Author: Rigano, P., primary, Manfré, L., additional, Galla, R. La, additional, Renda, D., additional, Renda, M. C., additional, Calabrese, A., additional, Calzolari, R., additional, and Maggio, A., additional
Published: 1997
Full Text: View/download PDF

17. Evidence for a Globin Promoter-Specific Silencer Element Located Upstream of the Human δ-Globin Gene

Author: Vitale, M., primary, Dimarzo, R., additional, Calzolari, R., additional, Acuto, S., additional, Oneill, D., additional, Bank, A., additional, and Maggio, A., additional
Published: 1994
Full Text: View/download PDF

18. Policies for the promotion of sustainable mobility and the reduction of traffic-related air pollution in the cities participating in the EpiAir2 project,Le politiche per la promozione della mobilità sostenibile e la riduzione dell'inquinamento atmosferico causato dal traffico veicolare nelle città partecipanti allo studio EpiAir2

Author: Di Lonardo, S., Nuvolone, D., Forastiere, F., Cadum, E., Barchielli, A., Accetta, G., Alessandrini, E. R., Angelini, P., Angiuli, L., Antonelli, A., Arena, P., Assennato, G., Baccini, M., FLAVIA BALDACCHINI, Baldacci, S., Baldini, M., Baldo, V., Ballarino, G., Balzi, D., Barban, I., Barbati, G., Barbone, F., Baroni, S., Barontini, R., Bartolacci, S., Bellodi, S., Berico, M., Berti, G., Biggeri, A., Bisanti, L., Bonfanti, M., Bonomolo, A., Bonvicini, L., Bovenzi, M., Brescianini, C., Broccoli, S., Bruni, A., Caldara, S., Calzolari, R., Canossa, E., Canova, C., Caramia, G., Carreras, G., Casale, G., Casale, P., Casella, C., Casetta, A., Cattani, G., Cernigliaro, A., Cerrai, S., Cervino, M., Cestari, L., Chellini, E., Chiusolo, M., Ciaccia, G., Cibella, F., Cocchio, S., Crosetto, L., Cuccaro, F., Daris, F., Donato, F., Girolamo, G., Togni, A., Di Biagio, K., Di Leo, A., Dessì, M. P., Evangelista, A., Faccini, F., Fallani, G., Faustini, A., Fruzzetti, R., Galassi, C., Gallo, G., Gallo, L., Gandini, M., Ganzi, A., Garrone, E., Gatti, G., Gherardi, B., Gianicolo, E. A. L., Giannini, S., Giua, R., Goldoni, C. A., Grechi, D., Grosa, M., Guerrini, N., Impallomeni, M., Iuzzolino, C., La Torretta, T., Lanzani, G., Lauriola, P., Leuci, P., Luberto, F., Lupi, A., Madonia, G., Maio, S., Malaguti, A., Mangia, C., Marchesi, S., Mariottini, M., Mariuz, M., Marotta, A., Martini, A., Menegotto, M., Merlo, F., Migliore, E., Minerba, S., Minichilli, F., Mircea, M., Morassuto, C., Nocioni, A., Nuzzi, R., Onorati, G., Padolecchia, G., Palazzi, B., Pandolfi, P., Parodi, S., Pasetti, P., Pensato, A., Piersanti, A., Piovesan, C., Pistollato, S., Pizzi, L., Pizzuti, R., Addario, S. P., Porcaro, L., Protti, M., Puppo, A., Raciti, M., Randi, G., Ranzi, A., Rosa, M., Rubini, G., Sacco, F., Santoro, M., Sarno, G., Scala, D., Scarinzi, C., Scondotto, S., Selle, V., Serinelli, M., Simeoni, R., Simonato, L., Simoni, M., Spagnolo, G., Stafoggia, M., Stagnaro, E., Starnino, G., Stivanello, E., Tominz, R., Troiano, F., Vercelli, M., Vianello, L., Viegi, G., Vigotti, M. A., Volta, C., Zanini, G., Zauli-Sajani, S., and Zero, F.

19. Air pollution and urgent hospital admissions in 25 Italian cities: Results from the EpiAir2 project,Inquinamento atmosferico e ricoveri ospedalieri urgenti in 25 città Italiane: Risultati del progetto EpiAir2

Author: Scarinzi, C., Alessandrini, E. R., Chiusolo, M., Galassi, C., Baldini, M., Serinelli, M., Pandolfi, P., Bruni, A., Biggeri, A., Togni, A., Carreras, G., Casella, C., Canova, C., Randi, G., Andrea Ranzi, Morassuto, C., Cernigliaro, A., Giannini, S., Lauriola, P., Minichilli, F., Gherardi, B., Zauli-Sajani, S., Stafoggia, M., Casale, P., Gianicolo, E. A. L., Piovesan, C., Tominz, R., Porcaro, L., Cadum, E., Accetta, G., Angelini, P., Angiuli, L., Antonelli, A., Arena, P., Assennato, G., Baccini, M., Baldacchini, F., Baldacci, S., Baldo, V., Ballarino, G., Balzi, D., Barban, I., Barbati, G., Barbone, F., Barchielli, A., Baroni, S., Barontini, R., Bartolacci, S., Bellodi, S., Berico, M., Berti, G., Bisanti, L., Bonfanti, M., Bonomolo, A., Bonvicini, L., Bovenzi, M., Brescianini, C., Broccoli, S., Caldara, S., Calzolari, R., Canossa, E., Caramia, G., Casale, G., Casetta, A., Cattani, G., Cerrai, S., Cervino, M., Cestari, L., Chellini, E., Ciaccia, G., Cibella, F., Cocchio, S., Crosetto, L., Cuccaro, F., Daris, F., Donato, F., Girolamo, G., Di Biagio, K., Di Leo, A., Di Lonardo, S., Dessì, M. P., Evangelista, A., Faccini, F., Fallani, G., Faustini, A., Forastiere, F., Fruzzetti, R., Gallo, G., Gallo, L., Gandini, M., Ganzi, A., Garrone, E., Gatti, G., Giua, R., Goldoni, C. A., Grechi, D., Grosa, M., Guerrini, N., Impallomeni, M., Iuzzolino, C., La Torretta, T., Lanzani, G., Leuci, P., Luberto, F., Lupi, A., Madonia, G., Maio, S., Malaguti, A., Mangia, C., Marchesi, S., Mariottini, M., Mariuz, M., Marotta, A., Martini, A., Menegotto, M., Merlo, F., Migliore, E., Minerba, S., Mircea, M., Nocioni, A., Nuvolone, D., Nuzzi, R., Onorati, G., Padolecchia, G., Palazzi, B., Parodi, S., Pasetti, P., Pensato, A., Piersanti, A., Pistollato, S., Pizzi, L., Pizzuti, R., Addario, S. P., Protti, M., Puppo, A., Raciti, M., Rosa, M., Rubini, G., Sacco, F., Santoro, M., Sarno, G., Scala, D., Scondotto, S., Selle, V., Simeoni, R., Simonato, L., Simoni, M., Spagnolo, G., Stagnaro, E., Starnino, G., Stivanello, E., Troiano, F., Vercelli, M., Vianello, L., Viegi, G., Vigotti, M. A., Volta, C., Zanini, G., and Zero, F.

20. Air pollution and urgent hospital admissions in 25 Italian cities: Results from the EpiAir2 project | Inquinamento atmosferico e ricoveri ospedalieri urgenti in 25 città Italiane: Risultati del progetto EpiAir2

Author: Scarinzi, C., Alessandrini, E. R., Chiusolo, M., Galassi, C., Baldini, M., Serinelli, M., Pandolfi, P., Bruni, A., Biggeri, A., Togni, A., Carreras, G., Casella, C., Canova, C., Randi, G., Ranzi, A., Morassuto, C., Cernigliaro, A., Giannini, S., Lauriola, P., Minichilli, F., Gherardi, B., Zauli-Sajani, S., Stafoggia, M., Casale, P., Gianicolo, E. A. L., Piovesan, C., Tominz, R., Porcaro, L., Cadum, E., Accetta, G., Angelini, P., Angiuli, L., Antonelli, A., Arena, P., Assennato, G., Baccini, M., Baldacchini, F., Baldacci, S., Baldo, V., Ballarino, G., Balzi, D., Barban, I., Barbati, G., Fabio Barbone, Barchielli, A., Baroni, S., Barontini, R., Bartolacci, S., Bellodi, S., Berico, M., Berti, G., Bisanti, L., Bonfanti, M., Bonomolo, A., Bonvicini, L., Bovenzi, M., Brescianini, C., Broccoli, S., Caldara, S., Calzolari, R., Canossa, E., Caramia, G., Casale, G., Casetta, A., Cattani, G., Cerrai, S., Cervino, M., Cestari, L., Chellini, E., Ciaccia, G., Cibella, F., Cocchio, S., Crosetto, L., Cuccaro, F., Daris, F., Donato, F., Girolamo, G., Di Biagio, K., Di Leo, A., Di Lonardo, S., Dessì, M. P., Evangelista, A., Faccini, F., Fallani, G., Faustini, A., Forastiere, F., Fruzzetti, R., Gallo, G., Gallo, L., Gandini, M., Ganzi, A., Garrone, E., Gatti, G., Giua, R., Goldoni, C. A., Grechi, D., Grosa, M., Guerrini, N., Impallomeni, M., Iuzzolino, C., La Torretta, T., Lanzani, G., Leuci, P., Luberto, F., Lupi, A., Madonia, G., Maio, S., Malaguti, A., Mangia, C., Marchesi, S., Mariottini, M., Mariuz, M., Marotta, A., Martini, A., Menegotto, M., Merlo, F., Migliore, E., Minerba, S., Mircea, M., Nocioni, A., Nuvolone, D., Nuzzi, R., Onorati, G., Padolecchia, G., Palazzi, B., Parodi, S., Pasetti, P., Pensato, A., Piersanti, A., Pistollato, S., Pizzi, L., Pizzuti, R., Addario, S. P., Protti, M., Puppo, A., Raciti, M., Rosa, M., Rubini, G., Sacco, F., Santoro, M., Sarno, G., Scala, D., Scondotto, S., Selle, V., Simeoni, R., Simonato, L., Simoni, M., Spagnolo, G., Stagnaro, E., Starnino, G., Stivanello, E., Troiano, F., Vercelli, M., Vianello, L., Viegi, G., Vigotti, M. A., Volta, C., Zanini, G., and Zero, F.

21. Environmental indicators in EpiAir2 project: Air quality data for epidemiological surveillance,Indicatori ambientali nello studio EpiAir2: I dati di qualità dell'aria per la sorveglianza epidemiologica

Author: Gandini, M., Berti, G., Cattani, G., Faustini, A., Scarinzi, C., Donato, F., Accetta, G., Angiuli, L., Caldara, S., Carreras, G., Casale, P., Di Biagio, K., Giannini, S., Iuzzolino, C., Lanzani, G., Lauriola, P., Leuci, P., Mariuz, M., Marchesi, S., Nocioni, A., Pistollato, S., Pizzi, L., Ranzi, A., Serinelli, M., Stagnaro, E., Vianello, L., Vigotti, M., Zauli-Sajani, S., Cadum, E., Alessandrini, E. R., Angelini, P., Antonelli, A., Arena, P., Assennato, G., Baccini, M., Baldacchini, F., Baldacci, S., Baldini, M., Baldo, V., Ballarino, G., Balzi, D., Barban, I., Barbati, G., Barbone, F., Barchielli, A., Baroni, S., Barontini, R., Bartolacci, S., Bellodi, S., Berico, M., Biggeri, A., Bisanti, L., Bonfanti, M., Bonomolo, A., Bonvicini, L., Bovenzi, M., Brescianini, C., Broccoli, S., Bruni, A., Calzolari, R., Canossa, E., Canova, C., Caramia, G., Casale, G., Casella, C., Casetta, A., Cernigliaro, A., Cerrai, S., Cervino, M., Cestari, L., Chellini, E., Chiusolo, M., Ciaccia, G., Cibella, F., Cocchio, S., Crosetto, L., Cuccaro, F., Daris, F., Girolamo, G., Togni, A., Di Leo, A., Di Lonardo, S., Dessì, M. P., Evangelista, A., Faccini, F., Fallani, G., Forastiere, F., Fruzzetti, R., Galassi, C., Gallo, G., Gallo, L., Ganzi, A., Garrone, E., Gatti, G., Gherardi, B., Gianicolo, E. A. L., Giua, R., Goldoni, C. A., Grechi, D., Grosa, M., Guerrini, N., Impallomeni, M., La Torretta, T., Ferdinando Luberto, Lupi, A., Madonia, G., Maio, S., Malaguti, A., Mangia, C., Mariottini, M., Marotta, A., Martini, A., Menegotto, M., Merlo, F., Migliore, E., Minerba, S., Minichilli, F., Mircea, M., Morassuto, C., Nuvolone, D., Onorati, R. N. G., Padolecchia, G., Palazzi, B., Pandolfi, P., Parodi, S., Pasetti, P., Pensato, A., Piersanti, A., Piovesan, C., Pizzuti, R., Addario, S. P., Porcaro, L., Protti, M., Puppo, A., Raciti, M., Randi, G., Rosa, M., Rubini, G., Sacco, F., Santoro, M., Sarno, G., Scala, D., Scondotto, S., Selle, V., Simeoni, R., Simonato, L., Simoni, M., Spagnolo, G., Stafoggia, M., Starnino, G., Stivanello, E., Tominz, R., Troiano, F., Vercelli, M., Viegi, G., Vigotti, M. A., Volta, C., Zanini, G., and Zero, F.

22. Short-term impact of air pollution among Italian cities covered by the EpiAir2 project,Impatto a breve termine dell'inquinamento dell'aria nelle città coperte dalla sorveglianza epidemiologica EpiAir2

Author: Baccini, M., Biggeri, A., Accetta, G., Alessandrini, E. R., Angelini, P., Angiuli, L., Antonelli, A., Arena, P., Assennato, G., Baldacchini, F., Baldacci, S., Baldini, M., Baldo, V., Ballarino, G., Balzi, D., Barban, I., Barbati, G., Barbone, F., Barchielli, A., Baroni, S., Barontini, R., Bartolacci, S., Bellodi, S., Berico, M., Berti, G., Bisanti, L., Bonfanti, M., Bonomolo, A., Bonvicini, L., Bovenzi, M., Brescianini, C., Broccoli, S., Bruni, A., Cadum, E., Caldara, S., Calzolari, R., Canossa, E., Canova, C., Caramia, G., Carreras, G., Casale, G., Casale, P., Casella, C., Casetta, A., Cattani, G., Cernigliaro, A., Cerrai, S., Cervino, M., Cestari, L., Chellini, E., Chiusolo, M., Ciaccia, G., Cibella, F., Cocchio, S., Crosetto, L., Cuccaro, F., Daris, F., Francesca K. de'Donato, Girolamo, G., Togni, A., Di Biagio, K., Di Leo, A., Di Lonardo, S., Dessì, M. P., Evangelista, A., Faccini, F., Fallani, G., Faustini, A., Forastiere, F., Fruzzetti, R., Galassi, C., Gallo, G., Gallo, L., Gandini, M., Ganzi, A., Garrone, E., Gatti, G., Gherardi, B., Gianicolo, E. A. L., Giannini, S., Giua, R., Goldoni, C. A., Grechi, D., Grosa, M., Guerrini, N., Impallomeni, M., Iuzzolino, C., La Torretta, T., Lanzani, G., Lauriola, P., Leuci, P., Luberto, F., Lupi, A., Madonia, G., Maio, S., Malaguti, A., Mangia, C., Marchesi, S., Mariottini, M., Mariuz, M., Marotta, A., Martini, A., Menegotto, M., Merlo, F., Migliore, E., Minerba, S., Minichilli, F., Mircea, M., Morassuto, C., Nocioni, A., Nuvolone, D., Nuzzi, R., Onorati, G., Padolecchia, G., Palazzi, B., Pandolfi, P., Parodi, S., Pasetti, P., Pensato, A., Piersanti, A., Piovesan, C., Pistollato, S., Pizzi, L., Pizzuti, R., Addario, S. P., Porcaro, L., Protti, M., Puppo, A., Raciti, M., Randi, G., Ranzi, A., Rosa, M., Rubini, G., Sacco, F., Santoro, M., Sarno, G., Scala, D., Scarinzi, C., Scondotto, S., Selle, V., Serinelli, M., Simeoni, R., Simonato, L., Simoni, M., Spagnolo, G., Stafoggia, M., Stagnaro, E., Starnino, G., Stivanello, E., Tominz, R., Troiano, F., Vercelli, M., Vianello, L., Viegi, G., Vigotti, M. A., Volta, C., Zanini, G., Zauli-Sajani, S., and Zero, F.

23. Evidence of induced non-tolerance in HLA-identical twins with hemoglobinopathy after in utero fetal transplantation

Author: Orlandi F, Giambona A, Messana F, Marino M, Abate I, Calzolari R, Damiani F, Jakil C, maria concetta renda, Dieli F, Buscemi F, Westgren M, Ringden O, and Maggio A
Subjects: Adolescent, Pregnancy, Histocompatibility Testing, beta-Thalassemia, Immune Tolerance, Humans, Female, Fetofetal Transfusion, T-Lymphocytes, Cytotoxic
Abstract: Fetus-to-fetus transplantation has been suggested for the treatment of hemoglobinopathies in utero. However, dissimilar results have to date been obtained by different groups. We describe a case in which fetus-to-fetus transplantation in HLA-identical twins was performed at the 19th week of gestation by infusion of 0.8 ml of fetal blood from normal to beta-thalassemia affected fetus with the main aim of inducing tolerance. No evidence of engraftment, determined by KM19 polymorphism, was present after 2 years of the procedure. Moreover, an alloreactive cytotoxic T lymphocyte precursor (CTLp) study of affected fetus vs donor and other different stimulators showed that immunization vs tolerance was the real effect of the procedure.

24. Short-term impact of air pollution among Italian cities covered by the EpiAir2 project | Impatto a breve termine dell'inquinamento dell'aria nelle città coperte dalla sorveglianza epidemiologica EpiAir2

Author: Baccini, M., Biggeri, A., Accetta, G., Alessandrini, E. R., Angelini, P., Angiuli, L., Antonelli, A., Arena, P., Assennato, G., Baldacchini, F., Baldacci, S., Baldini, M., Baldo, V., Ballarino, G., Balzi, D., Barban, I., Barbati, G., Barbone, F., Barchielli, A., Baroni, S., Barontini, R., Bartolacci, S., Bellodi, S., Berico, M., Berti, G., Bisanti, L., Bonfanti, M., Bonomolo, A., Bonvicini, L., Bovenzi, M., Brescianini, C., Broccoli, S., Bruni, A., Cadum, E., Caldara, S., Calzolari, R., Canossa, E., Canova, C., Caramia, G., Carreras, G., Casale, G., Casale, P., Casella, C., Casetta, A., Cattani, G., Cernigliaro, A., Cerrai, S., Cervino, M., Cestari, L., Chellini, E., Chiusolo, M., Ciaccia, G., Cibella, F., Cocchio, S., Crosetto, L., Cuccaro, F., Daris, F., Donato, F., Girolamo, G., Togni, A., Di Biagio, K., Di Leo, A., Di Lonardo, S., Dessì, M. P., Evangelista, A., Faccini, F., Fallani, G., Faustini, A., Forastiere, F., Fruzzetti, R., Galassi, C., Gallo, G., Gallo, L., Gandini, M., Ganzi, A., Garrone, E., Gatti, G., Gherardi, B., Gianicolo, E. A. L., Giannini, S., Giua, R., Goldoni, C. A., Grechi, D., Grosa, M., Guerrini, N., Impallomeni, M., Iuzzolino, C., La Torretta, T., Lanzani, G., Lauriola, P., Leuci, P., Luberto, F., Lupi, A., Madonia, G., Maio, S., Malaguti, A., Mangia, C., Marchesi, S., Mariottini, M., Mariuz, M., Marotta, A., Martini, A., Menegotto, M., Merlo, F., Migliore, E., Minerba, S., Fabrizio Minichilli, Mircea, M., Morassuto, C., Nocioni, A., Nuvolone, D., Nuzzi, R., Onorati, G., Padolecchia, G., Palazzi, B., Pandolfi, P., Parodi, S., Pasetti, P., Pensato, A., Piersanti, A., Piovesan, C., Pistollato, S., Pizzi, L., Pizzuti, R., Addario, S. P., Porcaro, L., Protti, M., Puppo, A., Raciti, M., Randi, G., Ranzi, A., Rosa, M., Rubini, G., Sacco, F., Santoro, M., Sarno, G., Scala, D., Scarinzi, C., Scondotto, S., Selle, V., Serinelli, M., Simeoni, R., Simonato, L., Simoni, M., Spagnolo, G., Stafoggia, M., Stagnaro, E., Starnino, G., Stivanello, E., Tominz, R., Troiano, F., Vercelli, M., Vianello, L., Viegi, G., Vigotti, M. A., Volta, C., Zanini, G., Zauli-Sajani, S., and Zero, F.

25. Air pollution and mortality in twenty-five Italian cities: Results of the EpiAir2 Project,Inquinamento atmosferico e mortalità in venticinque città Italiane: Risultati del progetto EpiAir2

Author: Alessandrini, E. R., Faustini, A., Chiusolo, M., Stafoggia, M., Gandini, M., Demaria, M., Antonelli, A., Arena, P., Biggeri, A., Canova, C., Casale, G., Cernigliaro, A., Garrone, E., Gherardi, B., Gianicolo, E. A. L., Giannini, S., Iuzzolino, C., Lauriola, P., Mariottini, M., Pasetti, P., Randi, G., Andrea Ranzi, Santoro, M., Selle, V., Serinelli, M., Stivanello, E., Tominz, R., Vigotti, M., Zauli-Sajani, S., Forastiere, F., Cadum, E., Accetta, G., Angelini, P., Angiuli, L., Assennato, G., Baccini, M., Baldacchini, F., Baldacci, S., Baldini, M., Baldo, V., Ballarino, G., Balzi, D., Barban, I., Barbati, G., Barbone, F., Barchielli, A., Baroni, S., Barontini, R., Bartolacci, S., Bellodi, S., Berico, M., Berti, G., Bisanti, L., Bonfanti, M., Bonomolo, A., Bonvicini, L., Bovenzi, M., Brescianini, C., Broccoli, S., Bruni, A., Caldara, S., Calzolari, R., Canossa, E., Caramia, G., Carreras, G., Casale, P., Casella, C., Casetta, A., Cattani, G., Cerrai, S., Cervino, M., Cestari, L., Chellini, E., Ciaccia, G., Cibella, F., Cocchio, S., Crosetto, L., Cuccaro, F., Daris, F., Donato, F., Girolamo, G., Togni, A., Di Biagio, K., Di Leo, A., Di Lonardo, S., Dessì, M. P., Evangelista, A., Faccini, F., Fallani, G., Fruzzetti, R., Galassi, C., Gallo, G., Gallo, L., Ganzi, A., Gatti, G., Giua, R., Goldoni, C. A., Grechi, D., Grosa, M., Guerrini, N., Impallomeni, M., La Torretta, T., Lanzani, G., Leuci, P., Luberto, F., Lupi, A., Madonia, G., Maio, S., Malaguti, A., Mangia, C., Marchesi, S., Mariuz, M., Marotta, A., Martini, A., Menegotto, M., Merlo, F., Migliore, E., Minerba, S., Minichilli, F., Mircea, M., Morassuto, C., Nocioni, A., Nuvolone, D., Nuzzi, R., Onorati, G., Padolecchia, G., Palazzi, B., Pandolfi, P., Parodi, S., Pensato, A., Piersanti, A., Piovesan, C., Pistollato, S., Pizzi, L., Pizzuti, R., Addario, S. P., Porcaro, L., Protti, M., Puppo, A., Raciti, M., Rosa, M., Rubini, G., Sacco, F., Sarno, G., Scala, D., Scarinzi, C., Scondotto, S., Simeoni, R., Simonato, L., Simoni, M., Spagnolo, G., Stagnaro, E., Starnino, G., Troiano, F., Vercelli, M., Vianello, L., Viegi, G., Vigotti, M. A., Volta, C., Zanini, G., and Zero, F.

26. Policies for the promotion of sustainable mobility and the reduction of traffic-related air pollution in the cities participating in the EpiAir2 project | Le politiche per la promozione della mobilità sostenibile e la riduzione dell'inquinamento atmosferico causato dal traffico veicolare nelle città partecipanti allo studio EpiAir2

Author: Di Lonardo, S., Nuvolone, D., Forastiere, F., Cadum, E., Barchielli, A., Accetta, G., Alessandrini, E. R., Angelini, P., Angiuli, L., Antonelli, A., Arena, P., Assennato, G., Baccini, M., Baldacchini, F., Baldacci, S., Baldini, M., Baldo, V., Ballarino, G., Balzi, D., Barban, I., Barbati, G., Fabio Barbone, Baroni, S., Barontini, R., Bartolacci, S., Bellodi, S., Berico, M., Berti, G., Biggeri, A., Bisanti, L., Bonfanti, M., Bonomolo, A., Bonvicini, L., Bovenzi, M., Brescianini, C., Broccoli, S., Bruni, A., Caldara, S., Calzolari, R., Canossa, E., Canova, C., Caramia, G., Carreras, G., Casale, G., Casale, P., Casella, C., Casetta, A., Cattani, G., Cernigliaro, A., Cerrai, S., Cervino, M., Cestari, L., Chellini, E., Chiusolo, M., Ciaccia, G., Cibella, F., Cocchio, S., Crosetto, L., Cuccaro, F., Daris, F., Donato, F., Girolamo, G., Togni, A., Di Biagio, K., Di Leo, A., Dessì, M. P., Evangelista, A., Faccini, F., Fallani, G., Faustini, A., Fruzzetti, R., Galassi, C., Gallo, G., Gallo, L., Gandini, M., Ganzi, A., Garrone, E., Gatti, G., Gherardi, B., Gianicolo, E. A. L., Giannini, S., Giua, R., Goldoni, C. A., Grechi, D., Grosa, M., Guerrini, N., Impallomeni, M., Iuzzolino, C., La Torretta, T., Lanzani, G., Lauriola, P., Leuci, P., Luberto, F., Lupi, A., Madonia, G., Maio, S., Malaguti, A., Mangia, C., Marchesi, S., Mariottini, M., Mariuz, M., Marotta, A., Martini, A., Menegotto, M., Merlo, F., Migliore, E., Minerba, S., Minichilli, F., Mircea, M., Morassuto, C., Nocioni, A., Nuzzi, R., Onorati, G., Padolecchia, G., Palazzi, B., Pandolfi, P., Parodi, S., Pasetti, P., Pensato, A., Piersanti, A., Piovesan, C., Pistollato, S., Pizzi, L., Pizzuti, R., Addario, S. P., Porcaro, L., Protti, M., Puppo, A., Raciti, M., Randi, G., Ranzi, A., Rosa, M., Rubini, G., Sacco, F., Santoro, M., Sarno, G., Scala, D., Scarinzi, C., Scondotto, S., Selle, V., Serinelli, M., Simeoni, R., Simonato, L., Simoni, M., Spagnolo, G., Stafoggia, M., Stagnaro, E., Starnino, G., Stivanello, E., Tominz, R., Troiano, F., Vercelli, M., Vianello, L., Viegi, G., Vigotti, M. A., Volta, C., Zanini, G., Zauli-Sajani, S., and Zero, F.

27. Air pollution and mortality in twenty-five Italian cities: Results of the EpiAir2 Project | Inquinamento atmosferico e mortalità in venticinque città Italiane: Risultati del progetto EpiAir2

Author: Alessandrini, E. R., Faustini, A., Chiusolo, M., Stafoggia, M., Gandini, M., Demaria, M., Antonelli, A., Arena, P., Biggeri, A., Canova, C., Casale, G., Cernigliaro, A., Garrone, E., Gherardi, B., Gianicolo, E. A. L., Giannini, S., Iuzzolino, C., Lauriola, P., Mariottini, M., Pasetti, P., Randi, G., Ranzi, A., Santoro, M., Selle, V., Serinelli, M., Stivanello, E., Tominz, R., Vigotti, M., Stefano Zauli Sajani, Forastiere, F., Cadum, E., Accetta, G., Angelini, P., Angiuli, L., Assennato, G., Baccini, M., Baldacchini, F., Baldacci, S., Baldini, M., Baldo, V., Ballarino, G., Balzi, D., Barban, I., Barbati, G., Barbone, F., Barchielli, A., Baroni, S., Barontini, R., Bartolacci, S., Bellodi, S., Berico, M., Berti, G., Bisanti, L., Bonfanti, M., Bonomolo, A., Bonvicini, L., Bovenzi, M., Brescianini, C., Broccoli, S., Bruni, A., Caldara, S., Calzolari, R., Canossa, E., Caramia, G., Carreras, G., Casale, P., Casella, C., Casetta, A., Cattani, G., Cerrai, S., Cervino, M., Cestari, L., Chellini, E., Ciaccia, G., Cibella, F., Cocchio, S., Crosetto, L., Cuccaro, F., Daris, F., Donato, F., Girolamo, G., Togni, A., Di Biagio, K., Di Leo, A., Di Lonardo, S., Dessì, M. P., Evangelista, A., Faccini, F., Fallani, G., Fruzzetti, R., Galassi, C., Gallo, G., Gallo, L., Ganzi, A., Gatti, G., Giua, R., Goldoni, C. A., Grechi, D., Grosa, M., Guerrini, N., Impallomeni, M., La Torretta, T., Lanzani, G., Leuci, P., Luberto, F., Lupi, A., Madonia, G., Maio, S., Malaguti, A., Mangia, C., Marchesi, S., Mariuz, M., Marotta, A., Martini, A., Menegotto, M., Merlo, F., Migliore, E., Minerba, S., Minichilli, F., Mircea, M., Morassuto, C., Nocioni, A., Nuvolone, D., Nuzzi, R., Onorati, G., Padolecchia, G., Palazzi, B., Pandolfi, P., Parodi, S., Pensato, A., Piersanti, A., Piovesan, C., Pistollato, S., Pizzi, L., Pizzuti, R., Addario, S. P., Porcaro, L., Protti, M., Puppo, A., Raciti, M., Rosa, M., Rubini, G., Sacco, F., Sarno, G., Scala, D., Scarinzi, C., Scondotto, S., Simeoni, R., Simonato, L., Simoni, M., Spagnolo, G., Stagnaro, E., Starnino, G., Troiano, F., Vercelli, M., Vianello, L., Viegi, G., Vigotti, M. A., Volta, C., Zanini, G., and Zero, F.

28. Impact of the environment on the health: From theory to practice

Author: A.L. Carducci, A. Agodi, C. Ancona, P. Angelini, F. Bagordo, F. Barbone, L. Birbaum, V. Carreri, A. Casuccio, A. Conti, M. Conversano, A. De Donno, O. De Giglio, F. Desiante, A. Di Pietro, E. Dogliotti, F. Donato, G.M. Fara, M. Fiore, F. Forastiere, G. Giammanco, A. Izzotti, M.T. Montagna, G. Oliveri Conti, M.G. Petronio, S. Sciacca, C. Signorelli, E. Testai, M. Verani, M. Vinceti, F. Vitale, M. Ferrante, G. Adani, L. Berghella, C. Calia, R. Calzolari, A. Canale, D. Castiglione, C. Copat, A. Cristaldi, G. Cuffari, G. Coronel Vargas, E. De Vita, F. De Nard, I. Federigi, T. Filippini, A. Grasso, N. Leonardi, M. Letzgus, G. Lo Bianco, W. Mazzucco, I. Nicolosi, P. Orlandi, G. Paladino, S. Pizzo, C. Pousis, M. Raffo, S. Rivolta, F. Scarpitta, G. Trani, F. Triggiano, A. Tumbarello, V. Vecchio, P. Zuccarello, M. Vassallo, Al, Carducci, Agodi, A, Ancona, C, Angelini, P, Bagordo, F, Barbone, F, Birbaum, L, V, Carreri, Casuccio, A, Conti, A, Conversano, M, De Donno, A, De Giglio, O, Desiante, F, Di Pietro, A, Dogliotti, E, Donato, F, Gm, Fara, Fiore, M, Forastiere, F, Giammanco, G, Izzotti, A, Montagna, M T, Oliveri Conti, G, Mg, Petronio, Sciacca, S, Signorelli, C, Testai, E, Verani, M, Vinceti, M, Vitale, F, Ferrante, M, Adani, G, Berghella, L, Calia, C, Calzolari, R, Canale, A, Castiglione, D, Copat, C, Cristaldi, A, Cuffari, G, Coronel Vargas, G, De Vita, E, De Nard, F, Federigi, I, Filippini, T, Grasso, A, Leonardi, N, Letzgus, M, Lo Bianco, G, Mazzucco, W, Nicolosi, I, Orlandi, P, Paladino, G, Pizzo, S, Pousis, C, Raffo, M, Rivolta, S, Scarpitta, F, Trani, G, Triggiano, F, Tumbarello, A, Vecchio, V, Zuccarello, P, M, Vassallo, Carducci, A. L., Agodi, A., Ancona, C., Angelini, P., Bagordo, F., Barbone, F., Birbaum, L., Carreri, V., Casuccio, A., Conti, A., Conversano, M., De Donno, A., De Giglio, O., Desiante, F., Di Pietro, A., Dogliotti, E., Donato, F., Fara, G. M., Fiore, M., Forastiere, F., Giammanco, G., Izzotti, A., Montagna, M. T., Oliveri Conti, G., Petronio, M. G., Sciacca, S., Signorelli, C., Testai, E., Verani, M., Vinceti, M., Vitale, F., Ferrante, M., Adani, G., Berghella, L., Calia, C., Calzolari, R., Canale, A., Castiglione, D., Copat, C., Cristaldi, A., Cuffari, G., Coronel Vargas, G., De Vita, E., De Nard, F., Federigi, I., Filippini, T., Grasso, A., Leonardi, N., Letzgus, M., Lo Bianco, G., Mazzucco, W., Nicolosi, I., Orlandi, P., Paladino, G., Pizzo, S., Pousis, C., Raffo, M., Rivolta, S., Scarpitta, F., Trani, G., Triggiano, F., Tumbarello, A., Vecchio, V., Zuccarello, P., and Vassallo, M.
Subjects: medicine.medical_specialty, Epidemiological and toxicological study, education, 010501 environmental sciences, Global Health, Settore MED/42 - Igiene Generale E Applicata, 01 natural sciences, Biochemistry, Risk communication, 03 medical and health sciences, 0302 clinical medicine, Environmental Impact Assessment, Health Impact Assessment, epidemiological and toxicological study, exposure assessment, risk communication, urban health, Political science, medicine, Global health, Humans, Environmental impact assessment, Exposure assessment, Health impact assessment, Urban health, 030212 general & internal medicine, Sicily, 0105 earth and related environmental sciences, General Environmental Science, Preventive healthcare, epidemiological and toxicological study exposure assessment, urban health risk communication, Medical education, Public health, Charter, Environmental Health, Public Health, One Health, Health Impact Assessment , Environmental Impact Assessment, epidemiological and toxicological study exposure assessment, urban health risk communication, Risk assessment, Working group
Abstract: The Erice 56 Charter titled "Impact of the environment on the health: from theory to practice" was unanimously approved at the end of the 56th course of the "International School of Epidemiology and Preventive Medicine G. D'Alessandro" held from 3th to 7th November 2019 in Erice - Sicily (Italy) and promoted by the Study Group of "Environment and Health" of the Italian Society of Hygiene, Preventive Medicine and Public Health. The course, that included lectures, open discussions and guided working groups, was aimed to provide a general training on epidemiological and toxicological aspects of the environmental health impact, to be used by public health professionals for risk assessment, without forgetting the risk communications. At the end of the course 12 key points were agreed among teachers and students: they underlined the need of specific training and research, in the perspective of "One Health" and "Global Health", also facing emerging scientific and methodological issues and focusing on communication towards stakeholders. This Discussion highlight the need to improve knowledge of Health and Environment topic in all sectors of health and environmental prevention and management.
Published: 2021

29. Off-label long acting injectable antipsychotics in real-world clinical practice: a cross-sectional analysis of prescriptive patterns from the STAR Network DEPOT study

Author: D'Agostino, Armando, Aguglia, Andrea, Barbui, Corrado, Bartoli, Francesco, Carrà, Giuseppe, Cavallotti, Simone, Chirico, Margherita, Ostinelli, Edoardo G, Zangani, Caroline, Martinotti, Giovanni, Ostuzzi, Giovanni, Bertolini, Federico, Calandra, Carmela, Callegari, Camilla, D’Agostino, Armando, Lucii, Claudio, Mastromo, Daniele, Moretti, Daniele, Monzani, Emiliano, Porcellana, Matteo, Prestia, Davide, Nose, Michela, Purgato, Marianna, Turrini, Giulia, Mazzi, Maria, Angela, Papola, Davide, Gastaldon, Chiara, Terlizzi, Samira, Piccoli, Alberto, Ruggeri, Mirella, De Fazio, Pasquale, Magliocco, Fabio, Caroleo, Mariarita, Raffaele, Gaetano, Ostinelli, Edoardo, Giuseppe, Bolognesi, Simone, Debolini, Sara, Pierantozzi, Elisa, Fargnoli, Francesco, Del Zanna, Maria, Giannini, Alessandra, Luccarelli, Livia, De Capua, Alberto, Annese, Pasqua, Maria, Cerretini, Massimiliano, Tozzi, Fiorella, Magnani, Nadia, Cardamone, Giuseppe, Bardicchia, Francesco, Facchi, Edvige, Soscia, Federica, Zotos, Spyridon, Biancosino, Bruno, Zonta, Filippo, Pompei, Francesco, Zizolfi, Daniele, Ielmini, Marta, Caselli, Ivano, Giana, Edoardo, Buzzi, Aldo, Emanuele, Diurni, Marcello, Milano, Anna, Sani, Emanuele, Calzolari, Roberta, Bortolaso, Paola, Piccinelli, Marco, Cazzamalli, Sara, Caterina, Alberini, Gabro, Piantanida, Silvia, Costantini, Chiara, Paronelli, Chiara, Di Caro, Angela, Moretti, Valentina, Gozzi, Mauro, D'Ippolito, Chiara, Barbanti Silva, Veronica, Papalini, Alessandro, Corbo, Mariangela, Campese, Ornella, Fiori, Federica, Lorusso, Marco, Di Capro, Lucia, Viceconte, Daniela, Mancini, Valerio, Suraniti, Francesco, Signorelli, Maria, Salvina, Rossi, Eugenio, Lupoli, Pasqualino, Menchetti, Marco, Terzi, Laura, Boso, Marianna, Risaro, Paolo, De Paoli, Giuseppe, Catania, Cristina, Tarricone, Ilaria, Caretto, Valentina, Storbini, Viviana, Emiliani, Roberta, Balzarro, Beatrice, Carra, Giuseppe, Tabacchi, Tommaso, Nava, Roberto, Bono, Adele, Provenzi, Milena, Brambilla, Giulia, Aspesi, Flora, Tremolada, Martina, Castagna, Gloria, Bava, Mattia, Verrengia, Enrica, Lucchi, Sara, Oriani, Maria, Ginevra, Barchiesi, Michela, Pacetti, Monica, Magni, Laura, Rosa, Rossi, Giuseppe, Beneduce, Rossella, Tura, Giovanni, Battista, Laffranchini, Laura, Ferrato, Farida, Restaino, Francesco, Limosani, Ivan, Ghio, Lucio, Ferro, Maurizio, Parise, Vincenzo, Fricchione, Balletta, Giovanni, Addeo, Lelio, De Vivo, Elisa, Di Benedetto, Rossella, Pinna, Federica, Carpiniello, Bernardo, Spano, Mariangela, Giacomin, Marzio, Pecile, Damiano, Mattei, Chiara, Fabrici, Elisabetta, Pascolo, Panarello, Sofia, Peresson, Giulia, Vitucci, Claudio, Bonavigo, Tommaso, Perini, Giovanni, Boschello, Filippo, Strizzolo, Stefania, Gardellin, Francesco, Di Giannantonio, Massimo, Fizzotti, Carlo, Cossetta, Edoardo, Di Gregorio, Luana, Sozzi, Francesca, Boncompagni, Giancarlo, La Barbera, Daniele, Colli, Giuseppe, Laurenzi, Sabrina, Luca, Maria, D'Agostino A., Aguglia A., Barbui C., Bartoli F., Carra G., Cavallotti S., Chirico M., Ostinelli E.G., Zangani C., Martinotti G., Ostuzzi G., Nose M., Purgato M., Turrini G., Mazzi M.A., Papola D., Gastaldon C., Terlizzi S., Bertolini F., Piccoli A., Ruggeri M., De Fazio P., Magliocco F., Caroleo M., Raffaele G., Bergamelli E., Lucii C., Bolognesi S., Debolini S., Pierantozzi E., Fargnoli F., Del Zanna M., Giannini A., Luccarelli L., De Capua A., Annese P.M., Cerretini M., Tozzi F., Magnani N., Cardamone G., Bardicchia F., Facchi E., Soscia F., Zotos S., Biancosino B., Zonta F., Pompei F., Callegari C., Zizolfi D., Poloni N., Ielmini M., Caselli I., Giana E., Buzzi A., Diurni M., Milano A., Sani E., Calzolari R., Bortolaso P., Piccinelli M., Cazzamalli S., Alberini G., Piantanida S., Costantini C., Paronelli C., Di Caro A., Moretti V., Gozzi M., D'Ippolito C., Barbanti S.V., Alessandro P., Corbo M., Campese O., Fiori F., Lorusso M., Di Capro L., Viceconte D., Mancini V., Suraniti F., Signorelli M.S., Rossi E., Lupoli P., Menchetti M., Terzi L., Boso M., Risaro P., De Paoli G., Catania C., Tarricone I., Caretto V., Storbini V., Emiliani R., Balzarro B., Tabacchi T., Nava R., Bono A., Provenzi M., Brambilla G., Aspesi F., Trotta G., Tremolada M., Castagna G., Bava M., Verrengia E., Lucchi S., Oriani M.G., Barchiesi M., Pacetti M., Amerio A., Amore M., Serafini G., Magni L.R., Rossi G., Beneduce R., Tura G.B., Laffranchini L., Mastromo D., Ferrato F., Restaino F., Monzani E., Porcellana M., Limosani I., Ghio L., Ferro M., Parise V.F., Balletta G., Addeo L., De Vivo E., Di Benedetto R., Pinna F., Carpiniello B., Spano M., Giacomin M., Pecile D., Mattei C., Fabrici E.P., Panarello S., Peresson G., Vitucci C., Bonavigo T., Perini G., Boschello F., Strizzolo S., Gardellin F., di Giannantonio M., Moretti D., Fizzotti C., Cossetta E., Di Gregorio L., Sozzi F., Boncompagni G., La Barbera D., Colli G., Laurenzi S., Calandra C., Luca M., D'Agostino, A, Aguglia, A, Barbui, C, Bartoli, F, Carra, G, Cavallotti, S, Chirico, M, Ostinelli, E, Zangani, C, Martinotti, G, Ostuzzi, G, D'Agostino, Armando, Aguglia, Andrea, Barbui, Corrado, Bartoli, Francesco, Carrà, Giuseppe, Cavallotti, Simone, Chirico, Margherita, Ostinelli, Edoardo G, Zangani, Caroline, Martinotti, Giovanni, Ostuzzi, Giovanni, and LA BARBERA, DANIELE
Subjects: Long-acting injectable antipsychotic, Cross-Sectional Studies, Off-label, Personality disorder, Bipolar disorder, Delayed-Action Preparations, Schizophrenia, Humans, Long-acting injectable antipsychotics, Off-Label Use, Antipsychotic Agents, Settore MED/25 - Psichiatria
Abstract: Introduction: Information on the off–label use of Long–Acting Injectable (LAI) antipsychotics in the real world is lacking. In this study, we aimed to identify the sociodemographic and clinical features of patients treated with on– vs off–label LAIs and predictors of off–label First– or Second–Generation Antipsychotic (FGA vs. SGA) LAI choice in everyday clinical practice. Method: In a naturalistic national cohort of 449 patients who initiated LAI treatment in the STAR Network Depot Study, two groups were identified based on off– or on–label prescriptions. A multivariate logistic regression analysis was used to test several clinically relevant variables and identify those associated with the choice of FGA vs SGA prescription in the off–label group. Results: SGA LAIs were more commonly prescribed in everyday practice, without significant differences in their on– and off–label use. Approximately 1 in 4 patients received an off–label prescription. In the off–label group, the most frequent diagnoses were bipolar disorder (67.5%) or any personality disorder (23.7%). FGA vs SGA LAI choice was significantly associated with BPRS thought disorder (OR = 1.22, CI95% 1.04 to 1.43, p= 0.015) and hostility/suspiciousness (OR = 0.83, CI95% 0.71 to 0.97, p= 0.017) dimensions. The likelihood of receiving an SGA LAI grew steadily with the increase of the BPRS thought disturbance score. Conversely, a preference towards prescribing an FGA was observed with higher scores at the BPRS hostility/suspiciousness subscale. Conclusion: Our study is the first to identify predictors of FGA vs SGA choice in patients treated with off–label LAI antipsychotics. Demographic characteristics, i.e. age, sex, and substance/alcohol use co–morbidities did not appear to influence the choice towards FGAs or SGAs. Despite a lack of evidence, clinicians tend to favour FGA over SGA LAIs in bipolar or personality disorder patients with relevant hostility. Further research is needed to evaluate treatment adherence and clinical effectiveness of these prescriptive patterns.
Published: 2022

30. Comparing 1-year effectiveness and acceptability of once-monthly paliperidone palmitate and aripiprazole monohydrate for schizophrenia spectrum disorders: Findings from the STAR Network Depot Study

Author: Francesco Bartoli, A, Daniele Cavaleri, A, Tommaso Callovini, A, Ilaria Riboldi, A, Cristina Crocamo, A, Armando D’Agostino, B, Giovanni Martinotti, C, Federico Bertolini, D, Giovanni Ostuzzi, D, Corrado Barbui, D, Giuseppe Carr`a, A, STAR Network Depot Investigators, Corrado, Barbui, Federico, Bertolini, Filippo, Boschello, Chiara, Gastaldon, Maria Angela Mazzi, Michela, Nos´e, Giovanni, Ostuzzi, Davide, Papola, Giovanni, Perini, Alberto, Piccoli, Michela, Pievani, Marianna, Purgato, Mirella, Ruggeri, Federico, Tedeschi, Samira, Terlizzi, and Giulia Turrini (Verona), Mariarita, Caroleo, Pasquale De Fazio, Fabio, Magliocco, and Gaetano Raffaele (Catanzaro), Simone, Cavallotti, Margherita, Chirico, Armando, D’Agostino, Farida, Ferrato, Ivan, Limosani, Daniele, Mastromo, Emiliano, Monzani, Edoardo Giuseppe Ostinelli, Matteo, Porcellana, and Francesco Restaino (Milano), Pasqua Maria Annese, Simone, Bolognesi, Massimiliano, Cerretini, Alberto De Capua, Sara, Debolini, Maria Del Zanna, Francesco, Fargnoli, Alessandra, Giannini, Livia, Luccarelli, Claudio, Lucii, Elisa, Pierantozzi, and Fiorella Tozzi (Siena), Francesco, Bardicchia, Giuseppe, Cardamone, Edvige, Facchi, Nadia, Magnani, and Federica Soscia (Grosseto), Bruno Biancosino, and Spyridon Zotos (Ferrara), Marzio, Giacomin, Francesco, Pompei, Mariangela, Spano, and Filippo Zonta (Treviso), Buzzi, ALDO EMANUELE, Callegari, Camilla, Roberta, Calzolari, Caselli, Ivano, Marcello, Diurni, Edoardo Luigi Giana, Ielmini, Marta, Anna, Milano, Emanuele, Sani, and Daniele Zizolfi (Varese), Gabrio, Alberini, Paola, Bortolaso, Sara, Cazzamalli, Chiara, Costantini, Angela Di Caro, Chiara, Paronelli, Silvia, Piantanida, and Marco Piccinelli (Varese Verbano), Papalini, Alessandro, Silva Veronica Barbanti, Chiara, D’Ippolito, Mauro, Gozzi, and Valentina Moretti (Reggio Emilia), Ornella, Campese, Mariangela, Corbo, Lucia Di Capro, Massimo di Giannantonio, Federica, Fiori, Marco, Lorusso, Valerio, Mancini, Giovanni, Martinotti, and Daniela Viceconte (Chieti), Carmela, Calandra, Maria, Luca, Maria Salvina Signorelli, and Francesco Suraniti (Catania), Beatrice, Balzarro, Giancarlo, Boncompagni, Valentina, Caretto, Roberta, Emiliani, Pasqualino, Lupoli, Marco, Menchetti, Eugenio, Rossi, Viviana, Storbini, Ilaria, Tarricone, and Laura Terzi (Bologna), Marianna, Boso, Cristina, Catania, Giuseppe De Paoli, and Paolo Risaro (Pavia), Flora, Aspesi, Francesco, Bartoli, Mattia, Bava, Adele, Bono, Giulia, Brambilla, Giuseppe, Carr`a, Gloria, Castagna, Sara, Lucchi, Roberto, Nava, Milena, Provenzi, Tommaso, Tabacchi, Martina, Tremolada, and Enrica Verrengia (Monza), Michela Barchiesi and Maria Ginevra Oriani (Ancona), Monica Pacetti (Forlì), Andrea, Aguglia, Maurizio, Ferro, and Lucio Ghio (Genova), Rossella, Beneduce, Laura, Laffranchini, Laura Rosa Magni, Giuseppe, Rossi, and Giovanni Battista Tura (Brescia), Lelio, Addeo, Giovanni, Balletta, Elisa De Vivo, Rossella Di Benedetto, and Vincenzo Fricchione Parise (Avellino), Bernardo Carpiniello and Federica Pinna (Cagliari), Damiano Pecile (Mantova), Chiara Mattei (Fermo), Tommaso, Bonavigo, Elisabetta Pascolo Fabrici, Sofia, Panarello, Giulia, Peresson, and Claudio Vitucci (Trieste), Francesco Gardellin, and Stefania Strizzolo (Vicenza), Edoardo, Cossetta, Carlo, Fizzotti, and Daniele Moretti (Savona), Luana Di Gregorio and Francesca Sozzi (Trento), Giuseppe Colli and Daniele La Barbera (Palermo), and Sabrina Laurenzi (Civitanova, Marche)., Bartoli, F, Cavaleri, D, Callovini, T, Riboldi, I, Crocamo, C, D'Agostino, A, Martinotti, G, Bertolini, F, Ostuzzi, G, Barbui, C, Carra, G, and Bartoli, F., Cavaleri, D., Callovini, T., Riboldi, I., Crocamo, C., D'Agostino, A., Martinotti, G., Bertolini, F., Ostuzzi, G., Barbui, C., Carrà, G., Boschello, F., Gastaldon, C., Mazzi, M.A., Nosé, M., Papola, D., Perini, G., Piccoli, A., Pievani, M., Purgato, M., Ruggeri, M., Tedeschi, F., Terlizzi, S., Turrini, G., Caroleo, M., De Fazio, P., Magliocco, F., Raffaele, G., Chirico, M., Ferrato, F., Limosani, I., Mastromo, D., Monzani, E., Ostinelli, E.G., Porcellana, M., Restaino, F., Annese, P.M., Bolognesi, S., Cerretini, M., De Capua, A., Debolini, S., Del Zanna, M., Fargnoli, F., Giannini, A., Luccarelli, L., Lucii, C., Pierantozzi, E., Tozzi, F., Bardicchia, F., Cardamone, G., Facchi, E., Magnani, N., Soscia, F., Biancosino, B., Zotos, S., Giacomin, M., Pompei, F., Spano, M., Zonta, F., Buzzi, A., Callegari, C., Calzolari, R., Caselli, I., Diurni, M., Giana, E.L., Ielmini, M., Milano, A., Poloni, N., Sani, E., Zizolfi, D., Alberini, G., Bortolaso, P., Cazzamalli, S., Costantini, C., Di Caro, A., Paronelli, C., Piantanida, S., Piccinelli, M., Alessandro, P., Barbanti, S.V., D'Ippolito, C., Gozzi, M., Moretti, V., Corbo, M., Di Capro, L., di Giannantonio, M., Fiori, F., Lorusso, M., Mancini, V., Viceconte, D., Calandra, C., Luca, M., Signorelli, M.S., Suraniti, F., Balzarro, B., Boncompagni, G., Caretto, V., Emiliani, R., Lupoli, P., Menchetti, M., Rossi, E., Storbini, V., Tarricone, I., Terzi, L., Boso, M., Catania, C., De Paoli, G., Risaro, P., Aspesi, F., Bava, M., Bono, A., Brambilla, G., Castagna, G., Lucchi, S., Nava, R., Provenzi, M., Tabacchi, T., Tremolada, M., Verrengia, E., Barchiesi, M., Oriani, M.G., Aguglia, A., Ferro, M., Ghio, L., Beneduce, R., Laffranchini, L., Magni, L.R., Rossi, G., Tura, G.B., Addeo, L., Balletta, G., De Vivo, E., Di Benedetto, R., Parise, V.F., Carpiniello, B., Pinna, F., Pecile, D., Mattei, C., Bonavigo, T., Fabrici, E.P., Panarello, S., Peresson, G., Vitucci, C., Pacetti, M., Gardellin, F., Strizzolo, S., Cossetta, E., Fizzotti, C., Moretti, D., Di Gregorio, L., Sozzi, F., Colli, G., La Barbera, D., Laurenzi, S.
Subjects: Long-acting injectable antipsychotic, Paliperidone palmitate 1-month, Aripiprazole, Aripiprazole monohydrate, Long-acting injectable antipsychotics, Psychiatry and Mental health, Paliperidone Palmitate, Schizophrenia, Humans, Prospective Studies, Settore MED/25 - Psichiatria, Biological Psychiatry, Antipsychotic Agents
Abstract: In this prospective study, we assessed the effectiveness and acceptability of paliperidone palmitate 1-month (PP1M) and aripiprazole monohydrate (AM) over 1-year follow-up. We included 195 subjects (117 treated with PP1M and 78 with AM) with schizophrenia spectrum disorders from real-world settings. We estimated no differences in hospitalization (Odds Ratio=1.59; p = 0.12), symptoms improvement (p = 0.90 adjusted for baseline severity), and discontinuation (Hazard Ratio=0.72; p = 0.20) at study endpoint. Although current evidence suggests the possible superiority of AM over PP1M, our findings showed comparable effectiveness between these drugs. Additional studies in real-world settings with direct comparisons between these two LAIs are needed.
Published: 2022

31. Factors associated with first- versus second-generation long-acting antipsychotics prescribed under ordinary clinical practice in Italy

Author: Ostuzzi, G., Mazzi, M. A., Terlizzi, S., Bertolini, F., Aguglia, A., Bartoli, F., Bortolaso, P., Callegari, C., Caroleo, M., Carrà, G., Corbo, M., D'Agostino, A., Gastaldon, C., Lucii, C., Magliocco, F., Martinotti, G., Nosé, M., Ostinelli, E. G., Papola, D., Piccinelli, M. P., Piccoli, A., Purgato, M., Tabacchi, T., Turrini, G., Ruggeri, M., Barbui, C., De Fazio, P., Raffaele, G., Chirico, M., Cavallotti, S., Bolognesi, S., Debolini, S., Pierantozzi, E., Fargnoli, F., Del Zanna, M., Giannini, A., Luccarelli, L., De Capua, A., Annese, P. M., Cerretini, M., Tozzi, F. E., Magnani, N., Cardamone, G., Bardicchia, F., Facchi, E., Soscia, F., Zotos, S., Biancosino, B., Zonta, F., Pompei, F., Zizolfi, D., Poloni, N., Ielmini, M., Caselli, I., Giana, E., Buzzi, A., Diurni, M., Milano, A., Sani, E., Calzolari, Roberta, Cazzamalli, S., Alberini, Gabrio, Piantanida, Silvia, Costantini, C., Paronelli, Chiara, Di Caro, A., Moretti, V., Gozzi, M., D'Ippolito, C., Barbanti, S. V., Alessandro, P., Campese, O., Fiori, F., Lorusso, M., Di Capro, L., Viceconte, D., Mancini, V., Suraniti, F., Signorelli, M. S., Rossi, E., Lupoli, P., Menchetti, M., Terzi, L., Boso, M., Risaro, P., De Paoli, G., Catania, C., Tarricone, I., Caretto, V., Storbini, V., Emiliani, R., Balzarro, B., Nava, R., Bono, A., Provenzi, M., Brambilla, G., Aspesi, F., Tremolada, M., Castagna, G., Bava, M., Verrengia, E., Lucchi, S., Oriani, M. G., Barchiesi, M., Pacetti, M., Magni, L. R., Rossi, G., Beneduce, R., Tura, G. B., Laffranchini, L., Mastromo, D., Ferrato, F., Restaino, F., Monzani, E., Porcellana, M., Limosani, I., Ghio, L., Ferro, M., Parise, V. F., Balletta, G., Addeo, L., De Vivo, E., Benedetto, R. D., Pinna, F., Carpiniello, B., Spano, M., Giacomin, M., Pecile, D., Mattei, C., Fabrici, E. P., Panarello, S., Peresson, G., Vitucci, C., Bonavigo, T., Perini, G., Boschello, F., Strizzolo, S., Gardellin, F., Di Giannantonio, M., Moretti, D., Fizzotti, C., Cossetta, E., Gregorio, L. D., Sozzi, F., Boncompagni, G., Barbera, D. L., Colli, G., Laurenzi, S., Calandra, C., Luca, M., Ostuzzi, Giovanni, Mazzi, Maria Angela, Terlizzi, Samira, Bertolini, Federico, Aguglia, Andrea, Bartoli, Francesco, Bortolaso, Paola, Callegari, Camilla, Caroleo, Mariarita, Carrà, Giuseppe, Corbo, Mariangela, D'Agostino, Armando, Gastaldon, Chiara, Lucii, Claudio, Magliocco, Fabio, Martinotti, Giovanni, Nosé, Michela, Ostinelli, Edoardo Giuseppe, Papola, Davide, Piccinelli, Marco Piero, Piccoli, Alberto, Purgato, Marianna, Tabacchi, Tommaso, Turrini, Giulia, Ruggeri, Mirella, Barbui, Corrado, De Fazio, Pasquale, Raffaele, Gaetano, Chirico, Margherita, Cavallotti, Simone, Bolognesi, Simone, Debolini, Sara, Pierantozzi, Elisa, Fargnoli, Francesco, Del Zanna, Maria, Giannini, Alessandra, Luccarelli, Livia, De Capua, Alberto, Annese, Pasqua Maria, Cerretini, Massimiliano, Tozzi, Fior-Ella, Magnani, Nadia, Cardamone, Giuseppe, Bardicchia, Francesco, Facchi, Edvige, Soscia, Federica, Zotos, Spyridon, Biancosino, Bruno, Zonta, Filippo, Pompei, Francesco, Zizolfi, Daniele, Poloni, Nicola, Ielmini, Marta, Caselli, Ivano, Giana, Edoardo, Buzzi, Aldo, Diurni, Marcello, Milano, Anna, Sani, Emanuele, Calzolari, Roberta, Piccinelli, Marco, Cazzamalli, Sara, Alberini, Gabrio, Piantanida, Silvia, Costantini, Chiara, Paronelli, Chiara, Di Caro, Angela, Moretti, Valentina, Gozzi, Mauro, D'Ippolito, Chiara, Barbanti, Silva Veronica, Alessandro, Papalini, Campese, Ornella, Fiori, Federica, Lorusso, Marco, Di Capro, Lucia, Viceconte, Daniela, Mancini, Valerio, Suraniti, Francesco, Signorelli, Maria Salvina, Rossi, Eugenio, Lupoli, Pasqualino, Menchetti, Marco, Terzi, Laura, Boso, Marianna, Risaro, Paolo, De Paoli, Giuseppe, Catania, Cristina, Tarricone, Ilaria, Caretto, Valentina, Storbini, Viviana, Emiliani, Roberta, Balzarro, Beatrice, Nava, Roberto, Bono, Adele, Provenzi, Milena, Brambilla, Giulia, Aspesi, Flora, Tremolada, Martina, Castagna, Gloria, Bava, Mattia, Verrengia, Enrica, Lucchi, Sara, Oriani, Maria Ginevra, Barchiesi, Michela, Pacetti, Monica, Magni, Laura Rosa, Rossi, Giuseppe, Beneduce, Rossella, Tura, Giovanni Battista, Laffranchini, Laura, Mastromo, Daniele, Ferrato, Farida, Restaino, Francesco, Monzani, Emiliano, Porcellana, Matteo, Limosani, Ivan, Ghio, Lucio, Ferro, Maurizio, Parise, Vincenzo Fricchione, Balletta, Giovanni, Addeo, Lelio, De Vivo, Elisa, Benedetto, Rossella Di, Pinna, Federica, Carpiniello, Bernardo, Spano, Mariangela, Giacomin, Marzio, Pecile, Damiano, Mattei, Chiara, Fabrici, Elisabetta Pascolo, Panarello, Sofia, Peresson, Giulia, Vitucci, Claudio, Bonavigo, Tommaso, Perini, Giovanni, Boschello, Filippo, Strizzolo, Stefania, Gardellin, Francesco, Di Giannantonio, Massimo, Moretti, Daniele, Fizzotti, Carlo, Cossetta, Edoardo, Gregorio, Luana Di, Sozzi, Francesca, Boncompagni, Giancarlo, Barbera, Daniele La, Colli, Giuseppe, Laurenzi, Sabrina, Calandra, Carmela, Luca, Maria, Ostuzzi, G, Mazzi, M, Terlizzi, S, Bertolini, F, Aguglia, A, Bartoli, F, Bortolaso, P, Callegari, C, Caroleo, M, Carrà, G, Corbo, M, D'Agostino, A, Gastaldon, C, Lucii, C, Magliocco, F, Martinotti, G, Nosé, M, Ostinelli, E, Papola, D, Piccinelli, M, Piccoli, A, Purgato, M, Tabacchi, T, Turrini, G, Ruggeri, M, Barbui, C, De Fazio, P, Raffaele, G, Chirico, M, Cavallotti, S, Bolognesi, S, Debolini, S, Pierantozzi, E, Fargnoli, F, Del Zanna, M, Giannini, A, Luccarelli, L, De Capua, A, Annese, P, Cerretini, M, Tozzi, F, Magnani, N, Cardamone, G, Bardicchia, F, Facchi, E, Soscia, F, Zotos, S, Biancosino, B, Zonta, F, Pompei, F, Zizolfi, D, Poloni, N, Ielmini, M, Caselli, I, Giana, E, Buzzi, A, Diurni, M, Milano, A, Sani, E, Calzolari, R, Cazzamalli, S, Alberini, G, Piantanida, S, Costantini, C, Paronelli, C, Di Caro, A, Moretti, V, Gozzi, M, D'Ippolito, C, Barbanti, S, Alessandro, P, Campese, O, Fiori, F, Lorusso, M, Di Capro, L, Viceconte, D, Mancini, V, Suraniti, F, Signorelli, M, Rossi, E, Lupoli, P, Menchetti, M, Terzi, L, Boso, M, Risaro, P, De Paoli, G, Catania, C, Tarricone, I, Caretto, V, Storbini, V, Emiliani, R, Balzarro, B, Nava, R, Bono, A, Provenzi, M, Brambilla, G, Aspesi, F, Tremolada, M, Castagna, G, Bava, M, Verrengia, E, Lucchi, S, Oriani, M, Barchiesi, M, Pacetti, M, Magni, L, Rossi, G, Beneduce, R, Tura, G, Laffranchini, L, Mastromo, D, Ferrato, F, Restaino, F, Monzani, E, Porcellana, M, Limosani, I, Ghio, L, Ferro, M, Parise, V, Balletta, G, Addeo, L, De Vivo, E, Benedetto, R, Pinna, F, Carpiniello, B, Spano, M, Giacomin, M, Pecile, D, Mattei, C, Fabrici, E, Panarello, S, Peresson, G, Vitucci, C, Bonavigo, T, Perini, G, Boschello, F, Strizzolo, S, Gardellin, F, Di Giannantonio, M, Moretti, D, Fizzotti, C, Cossetta, E, Gregorio, L, Sozzi, F, Boncompagni, G, Barbera, D, Colli, G, Laurenzi, S, Calandra, C, and Luca, M
Subjects: Genetics and Molecular Biology (all), Male, Pediatrics, European People, Bipolar Disorder, Cross-sectional study, Economics, Epidemiology, medicine.medical_treatment, assessment, viruses, lcsh:Medicine, Social Sciences, Longitudinal Studie, Biochemistry, Prescription, Geographical locations, 0302 clinical medicine, Mathematical and Statistical Techniques, Biochemistry, Genetics and Molecular Biology (all), Agricultural and Biological Sciences (all), immune system diseases, Medicine and Health Sciences, long-acting intramuscular (LAI) antipsychotics, clinical practice, second-generation antipsychotic (SGA) LAIs, Antipsychotics, Ethnicities, Longitudinal Studies, lcsh:Science, Multidisciplinary, virus diseases, Drugs, Middle Aged, Italian People, Europe, Prescriptions, Italy, Physical Sciences, Aripiprazole, Female, Bivariate Analysis, Statistics (Mathematics), medicine.drug, Human, Research Article, Antipsychotic Agents, Employment, Adult, medicine.medical_specialty, Adolescent, Research and Analysis Methods, 03 medical and health sciences, Mental Health and Psychiatry, medicine, Humans, Paliperidone, Bipolar disorder, European Union, Statistical Methods, Antipsychotic, Cross-Sectional Studie, Pharmacology, Risperidone, business.industry, Mood Disorders, lcsh:R, medicine.disease, 030227 psychiatry, Antipsychotic Agent, Cross-Sectional Studies, Labor Economics, Multivariate Analysis, Schizophrenia, Observational study, lcsh:Q, Population Groupings, People and places, business, 030217 neurology & neurosurgery, Mathematics
Abstract: Background For many years, long-acting intramuscular (LAI) antipsychotics have been prescribed predominantly to chronic and severe patients, as a last resort when other treatments failed. Recently, a broader and earlier use of LAIs, particularly second-generation LAIs, has been emphasized. To date, few studies attempted to frame how this change in prescribing took place in real-world practice. Therefore, this study aimed to describe the clinical features of patients prescribed with LAIs, and to explore possible prescribing differences between first- and second-generations LAIs under ordinary clinical practice in Italy. Methods The STAR Network "Depot" Study is an observational, longitudinal, multicenter study involving 35 centers in Italy. In the cross-sectional phase, patients prescribed with LAIs were consecutively recruited and assessed over a period of 12 months. Descriptive statistics and multivariable logistic regression analyses were employed. Results Of the 451 recruited patients, 61% were males. The level of social and working functioning was heterogeneous, as was the severity of disease. Seventy-two per cent of the patients had a diagnosis of the schizophrenia spectrum. Seventy per cent were prescribed with second-generation antipsychotic (SGA) LAIs (mostly paliperidone, aripiprazole and risperidone). Compared to first-generation antipsychotic (FGA) LAIs, patients prescribed with SGA LAIs were more often younger; employed; with a diagnosis of the schizophrenia spectrum or bipolar disorder; with higher levels of affective symptoms; with fewer LAI prescriptions in the past. Discussion LAIs' prescribing practices appear to be more flexible as compared to the past, although this change is mostly restricted to SGA LAIs.
Published: 2018

32. The Sea Urchin sns5 Insulator Protects Retroviral Vectors From Chromosomal Position Effects by Maintaining Active Chromatin Structure

Author: Danilo D'Apolito, Leda Ferro, Giovanni Spinelli, Roberta Calzolari, Elena Baiamonte, Mariella Bagliesi, Aurelio Maggio, S. Acuto, Vito Franco, Rosalba Di Marzo, D'Apolito, D, Baiamonte, E, Bagliesi, M, Di Marzo, R, Calzolari, R, Franco, V, Spinelli, G, Maggio, A, and Acuto, S
Subjects: Chromatin Immunoprecipitation, Euchromatin, Heterochromatin, Genetic Vectors, Settore BIO/11 - Biologia Molecolare, Settore MED/08 - Anatomia Patologica, Biology, Chromatin remodeling, Chromosomal Position Effects, Mice, Cell Line, Tumor, Drug Discovery, Genetics, Animals, Nucleosome, GATA1 Transcription Factor, Position Effect, Chromatin insulator, Molecular Biology, ChIA-PET, Pharmacology, Position Effects, Histone modifications, Original Articles, Chromatin, Retroviridae, Sea Urchins, NIH 3T3 Cells, Molecular Medicine, Insulator Elements, Chromatin immunoprecipitation, Octamer Transcription Factor-1, Protein Binding
Abstract: Silencing and position-effect (PE) variegation (PEV), which is due to integration of viral vectors in heterochromatin regions, are considered significant obstacles to obtaining a consistent level of transgene expression in gene therapy. The inclusion of chromatin insulators into vectors has been proposed to counteract this position-dependent variegation of transgene expression. Here, we show that the sea urchin chromatin insulator, sns5, protects a recombinant gamma-retroviral vector from the negative influence of chromatin in erythroid milieu. This element increases the probability of vector expression at different chromosomal integration sites, which reduces both silencing and PEV. By chromatin immunoprecipitation (ChIP) analysis, we demonstrated the specific binding of GATA1 and OCT1 transcription factors and the enrichment of hyperacetylated nucleosomes to sns5 sequences. The results suggest that this new insulator is able to maintain a euchromatin state inside the provirus locus with mechanisms that are common to other characterized insulators. On the basis of its ability to function as barrier element in erythroid milieu and to bind the erythroid specific factor GATA1, the inclusion of sns5 insulator in viral vectors may be of practical benefit in gene transfer applications and, in particular, for gene therapy of erythroid disorders.
Published: 2009
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33. Quantification of HBG mRNA in primary erythroid cultures: prediction of the response to hydroxyurea in sickle cell and beta-thalassemia

Author: Concetta Scazzone, Rosalba Di Marzo, Alice Pecoraro, Paolo Rigano, Roberta Calzolari, Aurelio Maggio, Antonio Troia, Martin H. Steinberg, Pecoraro, A, Rigano, P, Troia, A, Calzolari, R, Scazzone, C, Maggio, A, Steinberg, MH, and Di Marzo, R
Subjects: Adult, Male, congenital, hereditary, and neonatal diseases and abnormalities, Cell, Primary Cell Culture, Gene Expression, Anemia, Sickle Cell, Biology, Peripheral blood mononuclear cell, hydroxyurea, liquid erythroid culture, Young Adult, In vivo, hemic and lymphatic diseases, Fetal hemoglobin, medicine, Humans, gamma-Globins, RNA, Messenger, Fetal Hemoglobin, Aged, Erythroid Precursor Cells, Messenger RNA, beta-Thalassemia, Beta thalassemia, Hematology, General Medicine, Middle Aged, medicine.disease, b-thalassemia, Molecular biology, Real-time polymerase chain reaction, medicine.anatomical_structure, Treatment Outcome, Cell culture, Female, sickle cell disease
Abstract: Background and Objective Increased expression of fetal hemoglobin (HbF) may ameliorate the clinical course of hemoglobinopathies like sickle cell disease (SCD) and β-thalassemia. Hydroxyurea (HU) can stimulate HbF production in these diseases but the response is highly variable indicating the utility of developing an in vitro test to predict the patient's response to HU. We assessed whether the HbF response of patients with SCD and thalassemia intermedia (TI) to HU correlates with HBG (both γ-globin genes) expression in their cultured erythroid progenitors following exposure to HU. Patients and Methods We exposed primary erythroid cultures from peripheral blood mononuclear cells from 30 patients with SCD and 15 with TI to HU and measured HBG mRNA by real-time quantitative PCR. The same patients were then treated with HU and their HbF response after treatment with a stable dose of HU was compared with the mRNA results in cultured cells. Results and Conclusion The fold increase in HBG mRNA in erythroid progenitors was similar to the fold increase in HbF in vivo. Quantification of HBG mRNA in erythroid progenitor cell cultures from patients with SCD and TI is predictive of their clinical response to HU.
Published: 2014

34. Functional characterization of the sea urchin sns chromatin insulator in erythroid cells

Author: Giovanni Spinelli, Rosalba Di Marzo, Elena Baiamonte, Roberta Calzolari, S. Acuto, Aurelio Maggio, ACUTO S, DI MARZO R, CALZOLARI R, BAIAMONTE E, MAGGIO A, and SPINELLI G
Subjects: animal structures, Globin enhancer, Chromatin insulator, Enhancer blocking, Erythroid transcription factor, Sp1 Transcription Factor, Settore BIO/11 - Biologia Molecolare, Electrophoretic Mobility Shift Assay, DNA-binding protein, Paracentrotus lividus, Cell Line, Mice, Erythroid Cells, hemic and lymphatic diseases, biology.animal, Histone H2A, Animals, Humans, GATA1 Transcription Factor, Enhancer, Molecular Biology, Sea urchin, Transcription factor, biology, Gene Transfer Techniques, GATA1, Cell Biology, Hematology, biology.organism_classification, Locus Control Region, Molecular biology, Chromatin, Cell biology, Globins, Enhancer Elements, Genetic, Sea Urchins, Paracentrotus, Molecular Medicine, Insulator Elements, Octamer Transcription Factor-1
Abstract: Chromatin insulators are regulatory elements that determine domains of genetic functions. We have previously described the characterization of a 265 bp insulator element, termed sns, localized at the 3′ end of the early histone H2A gene of the sea urchin Paracentrotus lividus. This sequence contains three cis-acting elements (Box A, Box B, and Box C + T) all needed for the enhancer-blocking activity in both sea urchin and human cells. The goal of this study was to further characterize the sea urchin sns insulator in the erythroid environment. We employed colony assays in human (K562) and mouse (MEL) erythroid cell lines. We tested the capability of sns to interfere with the communication between the 5′HS2 enhancer of the human β-globin LCR and the γ-globin promoter. We found that the sns sequence displays directional enhancer-blocking activity. By the use of antibodies against known DNA binding proteins, in electrophoretic mobility shift assays, we demonstrated the binding of the erythroid-specific GATA-1 and the ubiquitous Oct-1 and Sp1 transcription factors. These factors bind to Box A, Box B, and Box C + T, respectively, in both K562 and MEL nuclear extracts. These results may have significant implications for the conservation of insulator function in evolutionary distant organisms and may prove to be of practical benefit in gene transfer applications for erythroid disorders such as hemoglobinopathies and thalassemias.
Published: 2005

35. Lexical experience with SALT

Author: Romary, Laurent, Human-machine dialogue with a significant language component (LANGUE ET DIALOGUE), INRIA Lorraine, Institut National de Recherche en Informatique et en Automatique (Inria)-Institut National de Recherche en Informatique et en Automatique (Inria)-Laboratoire Lorrain de Recherche en Informatique et ses Applications (LORIA), Institut National de Recherche en Informatique et en Automatique (Inria)-Université Henri Poincaré - Nancy 1 (UHP)-Université Nancy 2-Institut National Polytechnique de Lorraine (INPL)-Centre National de la Recherche Scientifique (CNRS)-Université Henri Poincaré - Nancy 1 (UHP)-Université Nancy 2-Institut National Polytechnique de Lorraine (INPL)-Centre National de la Recherche Scientifique (CNRS), and N. Calzolari, R. Grishman, M. Palmer
Subjects: ComputingMilieux_MISCELLANEOUS, [INFO.INFO-CL]Computer Science [cs]/Computation and Language [cs.CL]
Abstract: International audience
Published: 2002

36. How COVID-19 Affected Sleep Talking Episodes, Sleep and Dreams?

Author: Camaioni M, Scarpelli S, Alfonsi V, Gorgoni M, Calzolari R, De Bartolo M, Mangiaruga A, Couyoumdjian A, and De Gennaro L
Abstract: Background: The COVID-19 pandemic increased symptoms of stress and anxiety and induced changes in sleep quality, dream activity, and parasomnia episodes. It has been shown that stressful factors and/or bad sleep habits can affect parasomnia behaviors. However, investigations on how COVID-19 has affected sleep, dreams, and episode frequency in parasomnias are rare. The current study focuses on the impact of the pandemic on a specific parasomnia characterized by speech production (sleep talking, ST)., Methods: We selected 27 participants with frequent ST episodes (STs) during the pandemic and compared them with 27 participants with frequent STs from a previous study conducted during a pre-pandemic period. All participants performed home monitoring through sleep logs and recorded their nocturnal STs for one week., Results: We observed a higher frequency of STs in the pandemic group. Moreover, STs were related to the emotional intensity of dreams, independent of the pandemic condition. The pandemic was associated with lower bizarreness of dreams in the pandemic group. There were no differences in sleep variables between the two groups., Conclusion: Overall, these results suggest a stressful effect of COVID-19 on the frequency of STs. Both the pandemic and the frequency of STs affect qualitative characteristics of dreams in this population.
Published: 2024
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37. The Influence of Sleep Talking on Nocturnal Sleep and Sleep-Dependent Cognitive Processes.

Author: Camaioni M, Scarpelli S, Alfonsi V, Gorgoni M, De Bartolo M, Calzolari R, and De Gennaro L
Abstract: Background: Sleep talking (ST) is characterized by the production of unaware verbal vocal activations (VBs) during sleep. ST seems potentially linked to linguistic and memory consolidation processes. However, sleep and dream characteristics and the relationship between verbal vocalizations (VBs) and cognitive functions are still unknown. Our study aimed to investigate qualitative sleep and dream features in sleep talkers (STs) compared to healthy subjects (CNTs) through retrospective and longitudinal measures and explore the relationship between ST and memory consolidation., Methods: We recruited N = 29 STs and N = 30 CNTs (age range of 18-35). Participants recorded their dreams and filled out sleep logs for seven consecutive days. Vocal activations of STs were audio-recorded. On the eighth day, we administered a word-pair task., Results: We showed that STs had significantly worse self-reported sleep quality. VBs were positively correlated with sleep fragmentation and negatively associated with the oneiric emotional load. No difference between groups was found in the memory consolidation rate., Conclusions: Although ST is a benign phenomenon, we revealed that ST is associated with more sleep alterations and lower emotional intensity of dreams. In this vein, we support that ST depends on sleep fragmentation and could represent a potential window into sleep-dependent cognitive processes.
Published: 2022
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38. Short-term effects of desert and non-desert PM 10 on mortality in Sicily, Italy.

Author: Renzi M, Forastiere F, Calzolari R, Cernigliaro A, Madonia G, Michelozzi P, Davoli M, Scondotto S, and Stafoggia M
Subjects: Africa, Northern, Air Pollution analysis, Cities, Humans, Satellite Imagery, Seasons, Sicily epidemiology, Air Pollutants analysis, Mortality, Particulate Matter analysis
Abstract: Background: Increased PM 10 concentrations are commonly observed during Saharan dust advections. Limited epidemiological evidence suggests that PM 10 from anthropogenic and desert sources increase mortality. We aimed to evaluate the association between source-specific PM 10 (non-desert and desert) and cause-specific mortality in Sicily during 2006-2012 period., Methods: Daily PM 10 concentrations at 1-km 2 were estimated in Sicily using satellite-based data, fixed monitors and land use variables. We identified Saharan dust episodes using meteorological models, back-trajectories, aerosol maps, and satellite images. For each dust day, we estimated desert and non-desert PM 10 concentrations. We applied a time-series approach on 390 municipalities of Sicily to estimate the association between PM 10 (non-desert and desert) and daily cause-specific mortality., Results: 33% of all days were affected by Saharan dust advections. PM 10 concentrations were 8 μg/m 3 higher during dust days compared to other days. We found positive associations of both non-desert and desert PM 10 with cause-specific mortality. We estimated percent increases of risk (IR%) of non-accidental mortality equal to 2.3% (95% Confidence Interval [CI]: 1.4, 3.1) and 3.8% (3.2, 4.4), per 10 μg/m 3 increases in non-desert and desert PM 10 at lag 0-5, respectively. We also observed significant associations with cardiovascular (2.4% [1.3, 3.4] and 4.5% [3.8, 5.3]) and respiratory mortality (8.1% [6.8, 9.5], and 6.3% [5.4, 7.2]). We estimated higher effects during April-September, with IR% = 4.4% (3.2, 5.7) and 6.3% (5.4, 7.2) for non-desert and desert PM 10 , respectively., Conclusions: Our results confirm previous evidence of harmful effects of desert PM 10 on non-accidental and cardio-respiratory mortality, especially during the warm season., (Copyright © 2018 Elsevier Ltd. All rights reserved.)
Published: 2018
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39. Magnetic fields exposure from high-voltage power lines and risk of amyotrophic lateral sclerosis in two Italian populations.

Author: Vinceti M, Malagoli C, Fabbi S, Kheifets L, Violi F, Poli M, Caldara S, Sesti D, Violanti S, Zanichelli P, Notari B, Fava R, Arena A, Calzolari R, Filippini T, Iacuzio L, Arcolin E, Mandrioli J, Fini N, Odone A, Signorelli C, Patti F, Zappia M, Pietrini V, Oleari P, Teggi S, Ghermandi G, Dimartino A, Ledda C, Mauceri C, Sciacca S, Fiore M, and Ferrante M
Subjects: Age Distribution, Aged, Aged, 80 and over, Amyotrophic Lateral Sclerosis diagnosis, Female, Humans, Italy epidemiology, Male, Middle Aged, Prevalence, Radiation Dosage, Risk Factors, Sex Distribution, Amyotrophic Lateral Sclerosis epidemiology, Electricity, Magnetic Fields, Radiation Exposure analysis, Radiation Exposure statistics & numerical data, Radiation Injuries epidemiology
Abstract: The aetiology of amyotrophic lateral sclerosis (ALS), a rare and extremely severe neurodegenerative disease, has been associated with magnetic fields exposure. However, evidence for such a relation in the general population is weak, although the previous null results might also be due to exposure misclassification, or a relationship might exist only for selected subgroups. To test such a hypothesis we carried out a population-based case-control study in two Northern and Southern Italy regions, including 703 ALS cases newly diagnosed from 1998 to 2011 and 2737 controls randomly selected from the residents in the study provinces. Overall, we found that a residence near high-voltage power lines, within the corridors yielding a magnetic fields of ≥0.1 μT, was not associated with an excess disease risk, nor did we identify a dose-response relationship after splitting the exposed corridor according to the 0.1, 0.2 and 0.4 μT cut-points of exposure. These results were confirmed taking into account age at onset, period of diagnosis, sex, geographical area, and length of exposure. Overall, despite the residual possibility of unmeasured confounding or small susceptible subgroups not identified in our study, these results appear to confirm that the exposure to magnetic fields from power lines occurring in the general population is not associated with increased ALS risk.
Published: 2017
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40. Health effects of Saharan dust in Sicily Region (Southern Italy).

Author: Renzi M, Stafoggia M, Cernigliaro A, Calzolari R, Madonia G, Scondotto S, and Forastiere F
Subjects: Africa, Northern, Air Pollutants analysis, Cardiovascular Diseases mortality, Cities, Desert Climate, Female, Humans, Male, Respiratory Tract Diseases mortality, Sicily epidemiology, Time Factors, Air Pollution adverse effects, Cardiovascular Diseases epidemiology, Dust, Hospitalization statistics & numerical data, Particulate Matter analysis, Respiratory Tract Diseases epidemiology, Seasons
Abstract: "OBJECTIVES: to investigate the increase of PM10 during Saharan dust outbreaks with adverse health effects in Sicily (Southern Italy), the largest Mediterranean Island., Design: pooled analyses of time series with Poisson regression models to estimate the association between PM10 from different sources (desert and non-desert) and different outcomes., Setting and Participants: the four largest cities of Sicily (Palermo, Catania, Syracuse, and Messina) and three macroareas (North- East, South, and West) Sicily was divided into., Main Outcome Measures: daily count of cause-specific (ICD-9 codes) mortality and hospital admissions: natural (0-799), cardiovascular (390-459), and respiratory causes (460-519)., Results: 962 days affected by Saharan dust (30% of all days: 2,257) were identified. Significant associations between desert PM10 and natural mortality both in the cities and in the macro-areas were found, with increases of risk and 95% confidence intervals equal to 1.1% (95%CI 0.1-2.1) and 1.1% (95%CI 0.8-1.5) per 10 μg/m3 increase in lag 0-1 PM10, respectively. Weaker estimates were found for cardiorespiratory mortality. Desert PM10 displayed an association with respiratory hospitalizations, especially in the three macroareas (0.5%; 95%CI 0.1-1.0). In contrast, cardiovascular hospitalizations were associated only with non-desert PM10 in the four cities (1.3%; 95%CI 0.4- 2.1%). Higher desert PM10-related mortality was found during the warmer months (period: April-September): 2.7% (95%CI 0.8-4.5) in the four cities and 2.5% (95%CI 1.8%-3.2%) in the three macroareas. CONCLUSIONS PM10 originating from desert was positively associated with mortality and hospitalizations in Sicily. Policies should aim to reduce anthropogenic emissions even in areas with large contribution from desert sources."
Published: 2017
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41. Unrevealed Depression Involves Dysfunctional Coping Strategies in Crohn's Disease Patients in Clinical Remission.

Author: Viganò C, Calzolari R, Marinaccio PM, Bezzio C, Furfaro F, Ba G, and Maconi G
Abstract: Background and Aims. This study investigated the proportion of CD patients in clinical remission with clinical depression, and coping strategies in those with severe depressive disorders. Materials and Methods. One hundred consecutive CD patients in clinical remission were screened for anxiety and depression by using Hospital Anxiety and Depression Scale and patients with depressive symptoms were further investigated by means of Cognitive Behavioural Assessment 2.0 and Beck Depression Inventory (BDI). Afterwards the coping strategies were assessed through the Brief-COPE questionnaire. Results. Twenty-one patients had anxious symptoms and 16 had depressive symptoms with or without anxiety. Seven of these patients (43.8%) showed significant depressive symptoms. Compared to patients without psychiatric disorders, these patients showed significant lower score in "positive reframing" (p: 0.017) and in "planning" (p: 0.046) and higher score in "use of instrumental social support" (p < 0.001), in "denial" scale (p: 0.001), and in "use of emotional social support" (p: 0.003). Conclusions. Depressed CD patients in clinical remission may have dysfunctional coping strategies, meaning that they may not be able to implement functional strategies to manage at best stress related with their disease.
Published: 2016
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42. Efficacy of Rapamycin as Inducer of Hb F in Primary Erythroid Cultures from Sickle Cell Disease and β-Thalassemia Patients.

Author: Pecoraro A, Troia A, Calzolari R, Scazzone C, Rigano P, Martorana A, Sacco M, Maggio A, and Di Marzo R
Subjects: Adolescent, Adult, Aged, Anemia, Sickle Cell drug therapy, Anemia, Sickle Cell metabolism, Cells, Cultured, Female, Fetal Hemoglobin metabolism, Genotype, Humans, Hydroxyurea pharmacology, Hydroxyurea therapeutic use, Male, Middle Aged, Mutation, Young Adult, alpha-Globins genetics, alpha-Globins metabolism, beta-Globins genetics, beta-Globins metabolism, beta-Thalassemia drug therapy, beta-Thalassemia metabolism, gamma-Globins genetics, gamma-Globins metabolism, Anemia, Sickle Cell genetics, Erythroid Precursor Cells drug effects, Erythroid Precursor Cells metabolism, Fetal Hemoglobin genetics, Gene Expression Regulation drug effects, Sirolimus pharmacology, beta-Thalassemia genetics
Abstract: Phenotypic improvement of hemoglobinopathies such as sickle cell disease and β-thalassemia (β-thal) has been shown in patients with high levels of Hb F. Among the drugs proposed to increase Hb F production, hydroxyurea (HU) is currently the only one proven to improve the clinical course of these diseases. However, Hb F increase and patient's response are highly variable, indicating that new pharmacological agents could be useful for patients not responding to HU or showing a reduction of response during long-term therapy. In this study we evaluated the efficacy of rapamycin, a lypophilic macrolide used for the prevention of acute rejection in renal transplant recipients, as an inducer of Hb F production. The analyses were performed in cultured erythroid progenitors from 25 sickle cell disease and 25 β-thal intermedia (β-TI) patients. The use of a quantitative Real-Time-polymerase chain reaction ReTi-PCR technique and high performance liquid chromatography (HPLC) allowed us to determine the increase in γ-globin mRNA expression and Hb F production in human erythroid cells treated with rapamycin. The results of our study demonstrated an increase in vitro of γ-globin mRNA expression in 15 sickle cell disease and 14 β-TI patients and a corresponding Hb F increase. The induction by rapamycin, even if lower or similar in most of samples analyzed, in some cases was higher than HU. These data suggest that rapamycin could be a good candidate to be used in vivo for the treatment of hemoglobinopathies.
Published: 2015
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43. Quantification of HBG mRNA in primary erythroid cultures: prediction of the response to hydroxyurea in sickle cell and beta-thalassemia.

Author: Pecoraro A, Rigano P, Troia A, Calzolari R, Scazzone C, Maggio A, Steinberg MH, and Di Marzo R
Subjects: Adult, Aged, Anemia, Sickle Cell blood, Anemia, Sickle Cell drug therapy, Female, Fetal Hemoglobin genetics, Gene Expression, Humans, Hydroxyurea administration & dosage, Hydroxyurea therapeutic use, Male, Middle Aged, Primary Cell Culture, RNA, Messenger genetics, Treatment Outcome, Young Adult, beta-Thalassemia blood, beta-Thalassemia drug therapy, Anemia, Sickle Cell genetics, Erythroid Precursor Cells metabolism, beta-Thalassemia genetics, gamma-Globins genetics
Abstract: Background and Objective: Increased expression of fetal hemoglobin (HbF) may ameliorate the clinical course of hemoglobinopathies like sickle cell disease (SCD) and β-thalassemia. Hydroxyurea (HU) can stimulate HbF production in these diseases but the response is highly variable indicating the utility of developing an in vitro test to predict the patient's response to HU. We assessed whether the HbF response of patients with SCD and thalassemia intermedia (TI) to HU correlates with HBG (both γ-globin genes) expression in their cultured erythroid progenitors following exposure to HU., Patients and Methods: We exposed primary erythroid cultures from peripheral blood mononuclear cells from 30 patients with SCD and 15 with TI to HU and measured HBG mRNA by real-time quantitative PCR. The same patients were then treated with HU and their HbF response after treatment with a stable dose of HU was compared with the mRNA results in cultured cells., Results and Conclusion: The fold increase in HBG mRNA in erythroid progenitors was similar to the fold increase in HbF in vivo. Quantification of HBG mRNA in erythroid progenitor cell cultures from patients with SCD and TI is predictive of their clinical response to HU., (© 2013 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.)
Published: 2014
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44. Desensitization to hydroxycarbamide following long-term treatment of thalassaemia intermedia as observed in vivo and in primary erythroid cultures from treated patients.

Author: Rigano P, Pecoraro A, Calzolari R, Troia A, Acuto S, Renda D, Pantalone GR, Maggio A, and Di Marzo R
Subjects: Adolescent, Adult, Cells, Cultured, Drug Administration Schedule, Drug Tolerance, Erythropoiesis drug effects, Female, Fetal Hemoglobin biosynthesis, Follow-Up Studies, Genotype, Hematopoiesis, Extramedullary drug effects, Hemoglobins metabolism, Humans, Hydroxyurea administration & dosage, Hydroxyurea adverse effects, Male, Middle Aged, Treatment Outcome, Young Adult, beta-Thalassemia blood, beta-Thalassemia genetics, Erythroid Precursor Cells drug effects, Hydroxyurea therapeutic use, beta-Thalassemia drug therapy
Abstract: Hydroxycarbamide (HC) is a pharmacological agent capable of stimulating fetal haemoglobin (HbF) production during adult life. High levels of HbF may ameliorate the clinical course of β-thalassaemia and sickle cell disease. The efficacy of HC for the treatment of thalassaemia major and thalassaemia intermedia is variable. Although an increase of HbF has been observed in most patients, only some patients experience significant improvement in total haemoglobin levels. This study aimed to determine the effectiveness and safety of short- (1 year) and long-term (mean follow-up 68 months) HC treatment in 24 thalassaemia intermedia patients. Additionally, we evaluated if primary erythroid progenitor cells cultured from treated patients responded to HC treatment in a manner similar to that observed in vivo. Our results confirm a good response to HC after a short-term follow-up in 70% of thalassaemia intermedia patients and a reduction of clinical response in patients with a long follow-up. Erythroid cultures obtained from patients during treatment reproduced the observed in vivo response. Interestingly, haematopoietic stem cells from long-term treated patients showed reduced ability to develop into primary erythroid cultures some months before the reduction of the 'in vivo' response. The mechanism of this loss of response to HC remains to be determined., (© 2010 Blackwell Publishing Ltd.)
Published: 2010
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45. The sea urchin sns5 insulator protects retroviral vectors from chromosomal position effects by maintaining active chromatin structure.

Author: D'Apolito D, Baiamonte E, Bagliesi M, Di Marzo R, Calzolari R, Ferro L, Franco V, Spinelli G, Maggio A, and Acuto S
Subjects: Animals, Cell Line, Tumor, Chromatin Immunoprecipitation, Chromosomal Position Effects, GATA1 Transcription Factor metabolism, Insulator Elements genetics, Mice, NIH 3T3 Cells, Octamer Transcription Factor-1 metabolism, Protein Binding, Chromatin metabolism, Genetic Vectors genetics, Insulator Elements physiology, Retroviridae genetics, Sea Urchins genetics
Abstract: Silencing and position-effect (PE) variegation (PEV), which is due to integration of viral vectors in heterochromatin regions, are considered significant obstacles to obtaining a consistent level of transgene expression in gene therapy. The inclusion of chromatin insulators into vectors has been proposed to counteract this position-dependent variegation of transgene expression. Here, we show that the sea urchin chromatin insulator, sns5, protects a recombinant gamma-retroviral vector from the negative influence of chromatin in erythroid milieu. This element increases the probability of vector expression at different chromosomal integration sites, which reduces both silencing and PEV. By chromatin immunoprecipitation (ChIP) analysis, we demonstrated the specific binding of GATA1 and OCT1 transcription factors and the enrichment of hyperacetylated nucleosomes to sns5 sequences. The results suggest that this new insulator is able to maintain a euchromatin state inside the provirus locus with mechanisms that are common to other characterized insulators. On the basis of its ability to function as barrier element in erythroid milieu and to bind the erythroid specific factor GATA1, the inclusion of sns5 insulator in viral vectors may be of practical benefit in gene transfer applications and, in particular, for gene therapy of erythroid disorders.
Published: 2009
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46. Induction of gamma-globin gene transcription by hydroxycarbamide in primary erythroid cell cultures from Lepore patients.

Author: Calzolari R, Pecoraro A, Borruso V, Troia A, Acuto S, Maggio A, and Di Marzo R
Subjects: Cells, Cultured, Fetal Hemoglobin biosynthesis, Globins genetics, Humans, In Situ Hybridization, Fluorescence, RNA, Messenger genetics, Transcription, Genetic drug effects, beta-Thalassemia genetics, Erythroid Cells drug effects, Globins biosynthesis, Hemoglobins, Abnormal genetics, Hydroxyurea pharmacology, beta-Thalassemia blood
Abstract: Increased expression of fetal haemoglobin (HbF) may ameliorate the clinical course of beta-thalassemia and sickle cell disease. Some pharmacological agents, such as hydroxycarbamide (HC), can increase fetal haemoglobin synthesis during adult life. Cellular selection and/or molecular mechanisms have been proposed to account for this increase. To explore the mechanism of action of HC we focused on homozygous Hb-Lepore patients that presented with high fetal haemoglobin levels and were good responders to HC treatment "in vivo". We performed primary erythroid cultures from peripheral blood of four homozygous Lepore patients. The increase in HBG (gamma-globin) transcription levels and HbF content in these cultures, after HC treatment, were detected by quantitative real time polymerase chain reaction analysis and flow cytometric analysis. Primary transcript "in-situ" hybridization analysis showed a 2-fold increase in the number of cells expressing both HBG alleles in HC-treated erythroid cultures. These studies, demonstrating the larger number of biallelic HBG expressing cells, suggest that HC is able to stimulate the activation of HBG transcription. These observations provide evidences that the molecular mechanism of action is involved in the increase of fetal haemoglobin production by HC.
Published: 2008
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47. Functional characterization of the sea urchin sns chromatin insulator in erythroid cells.

Author: Acuto S, Di Marzo R, Calzolari R, Baiamonte E, Maggio A, and Spinelli G
Subjects: Animals, Cell Line, Chromatin genetics, Electrophoretic Mobility Shift Assay, Enhancer Elements, Genetic genetics, GATA1 Transcription Factor physiology, Gene Transfer Techniques, Globins genetics, Humans, Insulator Elements genetics, Locus Control Region physiology, Mice, Octamer Transcription Factor-1 physiology, Paracentrotus genetics, Sp1 Transcription Factor physiology, Chromatin physiology, Erythroid Cells metabolism, Insulator Elements physiology, Sea Urchins genetics
Abstract: Chromatin insulators are regulatory elements that determine domains of genetic functions. We have previously described the characterization of a 265 bp insulator element, termed sns, localized at the 3' end of the early histone H2A gene of the sea urchin Paracentrotus lividus. This sequence contains three cis-acting elements (Box A, Box B, and Box C + T) all needed for the enhancer-blocking activity in both sea urchin and human cells. The goal of this study was to further characterize the sea urchin sns insulator in the erythroid environment. We employed colony assays in human (K562) and mouse (MEL) erythroid cell lines. We tested the capability of sns to interfere with the communication between the 5'HS2 enhancer of the human beta-globin LCR and the gamma-globin promoter. We found that the sns sequence displays directional enhancer-blocking activity. By the use of antibodies against known DNA binding proteins, in electrophoretic mobility shift assays, we demonstrated the binding of the erythroid-specific GATA-1 and the ubiquitous Oct-1 and Sp1 transcription factors. These factors bind to Box A, Box B, and Box C + T, respectively, in both K562 and MEL nuclear extracts. These results may have significant implications for the conservation of insulator function in evolutionary distant organisms and may prove to be of practical benefit in gene transfer applications for erythroid disorders such as hemoglobinopathies and thalassemias.
Published: 2005
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48. Allele-specific transcription of fetal genes in primary erythroid cell cultures from Lepore and deltabeta degrees thalassemia patients.

Author: Di Marzo R, Acuto S, Calzolari R, and Maggio A
Subjects: Cells, Cultured, Gene Expression Regulation, Gene Silencing, Genotype, Hematopoietic Stem Cells metabolism, Humans, In Situ Hybridization, Mutation, Promoter Regions, Genetic, Alleles, Erythroid Cells metabolism, Fetal Hemoglobin genetics, Globins genetics, Thalassemia blood, Transcription, Genetic
Abstract: Objective: Autonomous gene silencing and gene competition by globin promoters for locus control region (LCR) function have been proposed as mechanisms in developmental regulation of beta-like genes. deltabeta degrees thalassemias are syndromes presenting an increased production of fetal hemoglobin in adult life; the majority of them are due to various deletions in beta-globin gene cluster. We studied samples from double heterozygotes for beta-thalassemia and for Lepore or Sicilian deltabeta degrees deletions, both lacking beta-promoter sequence. Our goal was to address the question of whether the allele carrying the deltabeta degrees deletion is responsible for high level of fetal hemoglobin (HbF) production., Patients and Methods: We analyzed the globin gene transcription in human erythroid cell cultures from peripheral blood stem cells, using primary transcript in situ hybridization. We performed primary erythroid cultures from patients with the following genotypes: Lepore/beta degrees 39, Sicilian deltabeta degrees /beta degrees 39, and, as controls, two thalassemia patients with nondeletional mutations (IVS1,6/IVS1,6; IVS1,6/beta degrees 39), and one normal individual., Results: The cells where it is possible to unambiguously assign gamma genes transcription in cis with the deletion (gamma:beta) are strongly represented with respect to the nine other combinations of gamma and beta hybridization signals. These cells are at least nine times more represented than those expressing the gamma allele in trans to the deletion., Conclusion: The allele-specific transcription of fetal genes in cis with the deletion is favored in both deletional genotypes. The absence of the adult promoter may influence LCR recruitment by fetal promoter, supporting the hypothesis that competition mechanism and gene silencing can coexist in regulating human globin gene transcription.
Published: 2005
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49. A region upstream of the human delta-globin gene shows a stage-specific interaction with globin promoters in erythroid cell lines.

Author: Vitale M, Calzolari R, Di Marzo R, Acuto S, and Maggio A
Subjects: Animals, Base Sequence, Binding Sites, DNA Footprinting, DNA-Binding Proteins metabolism, Fetal Proteins genetics, Humans, Mice, Transfection, Tumor Cells, Cultured, Erythroid Precursor Cells metabolism, Gene Expression Regulation, Developmental genetics, Globins genetics, Promoter Regions, Genetic genetics
Abstract: We previously showed that the 651-bp DNA fragment, located 3 kb upstream from the human delta-globin gene (fragment F5), is able to inhibit adult, not fetal, globin promoter in mouse erythroleukemia cell lines (MEL) expressing adult globin genes. Here we show in transient analysis that fragment F5 has a strong inhibitory effect on fetal gamma-globin promoter in human erythroleukemia cell lines (HEL) expressing fetal globin genes. Since the beta-promoter constructs were poorly expressed in fetal cells, new plasmids containing an HPFH promoter (Ggamma(-175), T to C), which is strongly expressed in both fetal and adult cell lines, were made. Here we report that fragment F5 in HEL cells has a strong inhibitory effect on wild-type gamma-promoter only; no effect was evident on gamma(-175)-promoter in either MEL or HEL cell lines. Altogether these results show a stage-specific interaction between fragment F5 and globin promoters during development. We also report the presence of several bindings for erythroid GATA family factors by electrophoretic mobility shift assay, using nuclear extracts from erythroid cell lines., ((c)2001 Elsevier Science.)
Published: 2001
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50. Intergenic transcription and developmental remodeling of chromatin subdomains in the human beta-globin locus.

Author: Gribnau J, Diderich K, Pruzina S, Calzolari R, and Fraser P
Subjects: Acetyltransferases metabolism, Cell Cycle, Chromatin ultrastructure, Gene Rearrangement, Histone Acetyltransferases, Humans, Liver metabolism, Locus Control Region, Models, Genetic, RNA Polymerase II metabolism, RNA, Messenger genetics, Chromatin metabolism, Chromosomes metabolism, Globins genetics, RNA, Messenger biosynthesis, Saccharomyces cerevisiae Proteins, Transcriptional Activation
Abstract: Gene activation requires chromatin remodeling complexes, which hyperacetylate histones and enable factor access; however, the targeting mechanisms leading to the establishment and maintenance of large, hyperacetylated DNase-sensitive chromatin domains are unknown. Recent work has shown that histone acetyltransferases are associated with RNA-pol II complexes, suggesting that transcription of chromatin plays a role in chromatin modification. Here we show the human beta-globin locus is divided into three differentially activated chromatin subdomains. Large transcripts precisely delineate the active domains at key cell cycle points associated with chromatin transitions and remodeling. We identify an element that initiates these transcripts, located in a region required for chromatin activation. The results suggest that intergenic transcription is required for chromatin remodeling of chromosomal domains.
Published: 2000
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