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1,615 results on '"Camussi, Giovanni"'

5. Stroma-derived miR-214 coordinates tumor dissemination

Author

Orso, Francesca, Virga, Federico, Dettori, Daniela, Dalmasso, Alberto, Paradzik, Mladen, Savino, Aurora, Pomatto, Margherita A. C., Quirico, Lorena, Cucinelli, Stefania, Coco, Martina, Mareschi, Katia, Fagioli, Franca, Salmena, Leonardo, Camussi, Giovanni, Provero, Paolo, Poli, Valeria, Mazzone, Massimiliano, Pandolfi, Pier Paolo, and Taverna, Daniela

Published
2023
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9. Multiomics of Tissue Extracellular Vesicles Identifies Unique Modulators of Atherosclerosis and Calcific Aortic Valve Stenosis

Author

Blaser, Mark C., Buffolo, Fabrizio, Halu, Arda, Turner, Mandy E., Schlotter, Florian, Higashi, Hideyuki, Pantano, Lorena, Clift, Cassandra L., Saddic, Louis A., Atkins, Samantha K., Rogers, Maximillian A., Pham, Tan, Vromman, Amélie, Shvartz, Eugenia, Sukhova, Galina K., Monticone, Silvia, Camussi, Giovanni, Robson, Simon C., Body, Simon C., Muehlschlegel, Jochen D., Singh, Sasha A., Aikawa, Masanori, and Aikawa, Elena

Published
2023
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11. Minimal information for studies of extracellular vesicles 2018 (MISEV2018): a position statement of the International Society for Extracellular Vesicles and update of the MISEV2014 guidelines

Author

Théry, Clotilde, Witwer, Kenneth W, Aikawa, Elena, Alcaraz, Maria Jose, Anderson, Johnathon D, Andriantsitohaina, Ramaroson, Antoniou, Anna, Arab, Tanina, Archer, Fabienne, Atkin‐Smith, Georgia K, Ayre, D Craig, Bach, Jean‐Marie, Bachurski, Daniel, Baharvand, Hossein, Balaj, Leonora, Baldacchino, Shawn, Bauer, Natalie N, Baxter, Amy A, Bebawy, Mary, Beckham, Carla, Zavec, Apolonija Bedina, Benmoussa, Abderrahim, Berardi, Anna C, Bergese, Paolo, Bielska, Ewa, Blenkiron, Cherie, Bobis‐Wozowicz, Sylwia, Boilard, Eric, Boireau, Wilfrid, Bongiovanni, Antonella, Borràs, Francesc E, Bosch, Steffi, Boulanger, Chantal M, Breakefield, Xandra, Breglio, Andrew M, Brennan, Meadhbh Á, Brigstock, David R, Brisson, Alain, Broekman, Marike LD, Bromberg, Jacqueline F, Bryl‐Górecka, Paulina, Buch, Shilpa, Buck, Amy H, Burger, Dylan, Busatto, Sara, Buschmann, Dominik, Bussolati, Benedetta, Buzás, Edit I, Byrd, James Bryan, Camussi, Giovanni, Carter, David RF, Caruso, Sarah, Chamley, Lawrence W, Chang, Yu‐Ting, Chen, Chihchen, Chen, Shuai, Cheng, Lesley, Chin, Andrew R, Clayton, Aled, Clerici, Stefano P, Cocks, Alex, Cocucci, Emanuele, Coffey, Robert J, Cordeiro‐da‐Silva, Anabela, Couch, Yvonne, Coumans, Frank AW, Coyle, Beth, Crescitelli, Rossella, Criado, Miria Ferreira, D'Souza‐Schorey, Crislyn, Das, Saumya, Chaudhuri, Amrita Datta, de Candia, Paola, De Santana, Eliezer F, De Wever, Olivier, del Portillo, Hernando A, Demaret, Tanguy, Deville, Sarah, Devitt, Andrew, Dhondt, Bert, Di Vizio, Dolores, Dieterich, Lothar C, Dolo, Vincenza, Rubio, Ana Paula Dominguez, Dominici, Massimo, Dourado, Mauricio R, Driedonks, Tom AP, Duarte, Filipe V, Duncan, Heather M, Eichenberger, Ramon M, Ekström, Karin, Andaloussi, Samir EL, Elie‐Caille, Celine, Erdbrügger, Uta, Falcón‐Pérez, Juan M, Fatima, Farah, Fish, Jason E, Flores‐Bellver, Miguel, Försönits, András, and Frelet‐Barrand, Annie

Subjects
Biochemistry and Cell Biology, Biological Sciences, extracellular vesicles, exosomes, ectosomes, microvesicles, minimal information requirements, guidelines, standardization, microparticles, rigor, reproducibility, Biochemistry and cell biology
Abstract

The last decade has seen a sharp increase in the number of scientific publications describing physiological and pathological functions of extracellular vesicles (EVs), a collective term covering various subtypes of cell-released, membranous structures, called exosomes, microvesicles, microparticles, ectosomes, oncosomes, apoptotic bodies, and many other names. However, specific issues arise when working with these entities, whose size and amount often make them difficult to obtain as relatively pure preparations, and to characterize properly. The International Society for Extracellular Vesicles (ISEV) proposed Minimal Information for Studies of Extracellular Vesicles ("MISEV") guidelines for the field in 2014. We now update these "MISEV2014" guidelines based on evolution of the collective knowledge in the last four years. An important point to consider is that ascribing a specific function to EVs in general, or to subtypes of EVs, requires reporting of specific information beyond mere description of function in a crude, potentially contaminated, and heterogeneous preparation. For example, claims that exosomes are endowed with exquisite and specific activities remain difficult to support experimentally, given our still limited knowledge of their specific molecular machineries of biogenesis and release, as compared with other biophysically similar EVs. The MISEV2018 guidelines include tables and outlines of suggested protocols and steps to follow to document specific EV-associated functional activities. Finally, a checklist is provided with summaries of key points.

Published
2018

14. Mesenchymal Stromal Cell-Derived Extracellular Vesicles for Reversing Hepatic Fibrosis in 3D Liver Spheroids.

Author

Chiabotto, Giulia, Semnani, Armina, Ceccotti, Elena, Guenza, Marco, Camussi, Giovanni, and Bruno, Stefania

Subjects
KUPFFER cells, TRANSFORMING growth factors-beta, HEPATIC fibrosis, LIVER cells, EXTRACELLULAR vesicles
Abstract

Hepatic fibrosis, arising from prolonged liver injury, entails the activation of hepatic stellate cells (HSCs) into myofibroblast-like cells expressing alpha-smooth muscle actin (α-SMA), thereby driving extracellular matrix deposition and fibrosis progression. Strategies targeting activated HSC reversal and hepatocyte regeneration show promise for fibrosis management. Previous studies suggest that extracellular vesicles (EVs) from mesenchymal stromal cells (MSCs) can suppress HSC activation, but ensuring EV purity is essential for clinical use. This study investigated the effects of MSC-derived EVs cultured in chemically defined conditions on liver spheroids and activated HSCs. Umbilical cord- and bone marrow-derived MSCs were expanded in chemically defined media, and EVs were isolated using filtration and differential ultracentrifugation. The impact of MSC-EVs was evaluated on liver spheroids generated in Sphericalplate 5D™ and on human HSCs, both activated by transforming growth factor beta 1 (TGF-β1). MSC-EVs effectively reduced the expression of profibrotic markers in liver spheroids and activated HSCs induced by TGF-β1 stimulation. These results highlight the potential of MSC-EVs collected under chemically defined conditions to mitigate the activated phenotype of HSCs and liver spheroids, suggesting MSC-EVs as a promising treatment for hepatic fibrosis. [ABSTRACT FROM AUTHOR]

Published
2024
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15. Extracellular Vesicles Derived from Human Liver Stem Cells Counteract Chronic Kidney Disease Development and Cardiac Dysfunction in Remnant Kidney Murine Model: The Possible Involvement of Proteases.

Author

Ceccotti, Elena, Chiabotto, Giulia, Cedrino, Massimo, Gambella, Alessandro, Delsedime, Luisa, Ghigo, Alessandra, Salio, Chiara, Grange, Cristina, Herrera Sanchez, Maria Beatriz, Femminò, Saveria, Sassoè-Pognetto, Marco, Brizzi, Maria Felice, Camussi, Giovanni, and Bruno, Stefania

Subjects
MATRIX metalloproteinases, HUMAN stem cells, GLOMERULOSCLEROSIS, KIDNEY development, CHRONIC kidney failure
Abstract

Fibrosis is a marker of chronic kidney disease (CKD) and consists of the accumulation of the extracellular matrix (ECM) components, causing the progressive deterioration of kidney function. Human liver stem cells (HLSCs) have anti-fibrotic activity, and HLSC-derived extracellular vesicles (EVs) mediate this effect. Herein, we evaluated the ability of HLSC-EVs to reverse renal and cardiac alterations in a murine model of partial nephrectomy (PNx) that mimics human CKD development. Furthermore, we investigated the contribution of extracellular matrix remodeling-related proteases to the anti-fibrotic effect of HLSC-EVs. PNx was performed by ligation of both poles of the left kidney, followed one week later by the removal of the right kidney. EV treatment started 4 weeks after the nephrectomy, when renal and cardiac alternations were already established, and mice were sacrificed at week eight. HLSC-EV treatment improved renal function and morphology, significantly decreasing interstitial fibrosis, glomerular sclerosis, and capillary rarefaction. This improvement was confirmed by the decreased expression of pro-fibrotic genes. Moreover, EV treatment improved cardiac function and reduced cardiac fibrosis. HLSC-EVs shuttled different proteases with ECM remodeling activity, and matrix metalloproteinase 1 (MMP-1) was involved in their anti-fibrotic effect on renal tissue. HLSC-EV treatment interferes with CKD development and ameliorates cardiomyopathy in PNx mice. [ABSTRACT FROM AUTHOR]

Published
2024
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22. In vitro characterization of 3D culture-based differentiation of human liver stem cells

Author

Tapparo, Marta, primary, Saccu, Gabriele, additional, Pasquino, Chiara, additional, Fonsato, Valentina, additional, Medana, Claudio, additional, Schiavo, Valentina, additional, Mecarelli, Enrica, additional, Maccagno, Monica, additional, Silengo, Lorenzo, additional, Bruno, Stefania, additional, Camussi, Giovanni, additional, and Herrera Sanchez, Maria Beatriz, additional

Published
2024
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23. Intrahepatic Administration of Human Liver Stem Cells in Infants with Inherited Neonatal-Onset Hyperammonemia: A Phase I Study

Author

Spada, Marco, Porta, Francesco, Righi, Dorico, Gazzera, Carlo, Tandoi, Francesco, Ferrero, Ivana, Fagioli, Franca, Sanchez, Maria Beatriz Herrera, Calvo, Pier Luigi, Biamino, Elisa, Bruno, Stefania, Gunetti, Monica, Contursi, Cristina, Lauritano, Carola, Conio, Alessandra, Amoroso, Antonio, Salizzoni, Mauro, Silengo, Lorenzo, Camussi, Giovanni, and Romagnoli, Renato

Published
2020
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24. Potential functional applications of extracellular vesicles: a report by the NIH Common Fund Extracellular RNA Communication Consortium.

Author

Quesenberry, Peter J, Aliotta, Jason, Camussi, Giovanni, Abdel-Mageed, Asim B, Wen, Sicheng, Goldberg, Laura, Zhang, Huang-Ge, Tetta, Ciro, Franklin, Jeffrey, Coffey, Robert J, Danielson, Kirsty, Subramanya, Vinita, Ghiran, Ionita, Das, Saumya, Chen, Clark C, Pusic, Kae M, Pusic, Aya D, Chatterjee, Devasis, Kraig, Richard P, Balaj, Leonora, and Dooner, Mark

Subjects
cancer, cell fate change, extracellular vesicles, functional effects, pulmonary heart disease, renal, Biochemistry and Cell Biology
Abstract

The NIH Extracellular RNA Communication Program's initiative on clinical utility of extracellular RNAs and therapeutic agents and developing scalable technologies is reviewed here. Background information and details of the projects are presented. The work has focused on modulation of target cell fate by extracellular vesicles (EVs) and RNA. Work on plant-derived vesicles is of intense interest, and non-mammalian sources of vesicles may represent a very promising source for different therapeutic approaches. Retro-viral-like particles are intriguing. Clearly, EVs share pathways with the assembly machinery of several other viruses, including human endogenous retrovirals (HERVs), and this convergence may explain the observation of viral-like particles containing viral proteins and nucleic acid in EVs. Dramatic effect on regeneration of damaged bone marrow, renal, pulmonary and cardiovascular tissue is demonstrated and discussed. These studies show restoration of injured cell function and the importance of heterogeneity of different vesicle populations. The potential for neural regeneration is explored, and the capacity to promote and reverse neoplasia by EV exposure is described. The tremendous clinical potential of EVs underlies many of these projects, and the importance of regulatory issues and the necessity of general manufacturing production (GMP) studies for eventual clinical trials are emphasized. Clinical trials are already being pursued and should expand dramatically in the near future.

Published
2015

25. Distinct profile of CD34+ cells and plasma-derived extracellular vesicles from triple-negative patients with Myelofibrosis reveals potential markers of aggressive disease

Author

Forte, Dorian, Barone, Martina, Morsiani, Cristina, Simonetti, Giorgia, Fabbri, Francesco, Bruno, Samantha, Bandini, Erika, Sollazzo, Daria, Collura, Salvatore, Deregibus, Maria Chiara, Auteri, Giuseppe, Ottaviani, Emanuela, Vianelli, Nicola, Camussi, Giovanni, Franceschi, Claudio, Capri, Miriam, Palandri, Francesca, Cavo, Michele, and Catani, Lucia

Published
2021
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28. mRNA Technology and Mucosal Immunization.

Author

Toniolo, Antonio, Maccari, Giuseppe, and Camussi, Giovanni

Subjects
MESSENGER RNA, HUMAN-to-human transmission, EXTRACELLULAR vesicles, RNA polymerases, COMBINED vaccines
Abstract

Current mRNA vaccines are mainly administered via intramuscular injection, which induces good systemic immunity but limited mucosal immunity. Achieving mucosal immunity through mRNA vaccination could diminish pathogen replication at the entry site and reduce interhuman transmission. However, delivering mRNA vaccines to mucosae faces challenges like mRNA degradation, poor entry into cells, and reactogenicity. Encapsulating mRNA in extracellular vesicles may protect the mRNA and reduce reactogenicity, making mucosal mRNA vaccines possible. Plant-derived extracellular vesicles from edible fruits have been investigated as mRNA carriers. Studies in animals show that mRNA vehiculated in orange-derived extracellular vesicles can elicit both systemic and mucosal immune responses when administered by the oral, nasal, or intramuscular routes. Once lyophilized, these products show remarkable stability. The optimization of mRNA to improve translation efficiency, immunogenicity, reactogenicity, and stability can be obtained through adjustments of the 5′cap region, poly-A tail, codons selection, and the use of nucleoside analogues. Recent studies have also proposed self-amplifying RNA vaccines containing an RNA polymerase as well as circular mRNA constructs. Data from parenterally primed animals demonstrate the efficacy of nasal immunization with non-adjuvanted protein, and studies in humans indicate that the combination of a parenteral vaccine with the natural exposure of mucosae to the same antigen provides protection and reduces transmission. Hence, mucosal mRNA vaccination would be beneficial at least in organisms pre-treated with parenteral vaccines. This practice could have wide applications for the treatment of infectious diseases. [ABSTRACT FROM AUTHOR]

Published
2024
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48. Extracellular vesicles from human plasma for biomarkers discovery: Impact of anticoagulants and isolation techniques

Author

Bettio, Valentina, primary, Mazzucco, Eleonora, additional, Antona, Annamaria, additional, Cracas, Silvia, additional, Varalda, Marco, additional, Venetucci, Jacopo, additional, Bruno, Stefania, additional, Chiabotto, Giulia, additional, Venegoni, Chiara, additional, Vasile, Alessandra, additional, Chiocchetti, Annalisa, additional, Quaglia, Marco, additional, Camussi, Giovanni, additional, Cantaluppi, Vincenzo, additional, Panella, Massimiliano, additional, Rolla, Roberta, additional, Manfredi, Marcello, additional, and Capello, Daniela, additional

Published
2023
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