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136 results on '"Camuzeaux, Stephane"'

2. Improved quantitation of short-chain carboxylic acids in human biofluids using 3-nitrophenylhydrazine derivatization and liquid chromatography with tandem mass spectrometry (LC-MS/MS)

Author

Valdivia-Garcia, Maria A., Chappell, Katie E., Camuzeaux, Stephane, Olmo-García, Lucía, van der Sluis, Verena Horneffer, Radhakrishnan, Shiva T., Stephens, Hannah, Bouri, Sonia, de Campos Braz, Lucia M., Williams, Horace T., Lewis, Matthew R., Frost, Gary, and Li, Jia V.

Published
2022
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4. Mass spectrometry imaging highlights dynamic patterns of lipid co‐expression with Aβ plaques in mouse and human brains

Author

Huang, Helen Xuexia, primary, Inglese, Paolo, additional, Tang, Jiabin, additional, Yagoubi, Riad, additional, Correia, Gonçalo D. S., additional, Horneffer‐van der Sluis, Verena M., additional, Camuzeaux, Stephane, additional, Wu, Vincen, additional, Kopanitsa, Maksym V., additional, Willumsen, Nanet, additional, Jackson, Johanna S., additional, Barron, Anna M., additional, Saito, Takashi, additional, Saido, Takaomi C., additional, Gentlemen, Steve, additional, Takats, Zoltan, additional, and Matthews, Paul M., additional

Published
2024
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5. Diet shapes the metabolite profile in the intact human ileum, which affects PYY release.

Author

Dagbasi, Aygul, Byrne, Claire, Blunt, Dominic, Serrano-Contreras, Jose Ivan, Becker, Georgia Franco, Blanco, Jesus Miguens, Camuzeaux, Stephane, Chambers, Edward, Danckert, Nathan, Edwards, Cathrina, Bernal, Andres, Garcia, Maria Valdivia, Hanyaloglu, Aylin, Holmes, Elaine, Ma, Yue, Marchesi, Julian, Martinez-Gili, Laura, Mendoza, Lilian, Tashkova, Martina, and Perez-Moral, Natalia

Subjects
ILEUM, HIGH-fiber diet, BACTERIAL metabolites, APPETITE stimulants, DIETARY fiber, GASTROINTESTINAL system, GLUCAGON-like peptides
Abstract

The human ileum contains a high density of enteroendocrine L-cells, which release the appetite-suppressing hormones glucagon-like peptide-1 (GLP-1) and peptide tyrosine tyrosine (PYY) in response to food intake. Recent evidence highlighted the potential role of food structures in PYY release, but the link between food structures, ileal metabolites, and appetite hormone release remains unclear owing to limited access to intact human ileum. In a randomized crossover trial (ISRCTN11327221; isrctn.com), we investigated the role of human ileum in GLP-1 and PYY release by giving healthy volunteers diets differing in fiber and food structure: high-fiber (intact or disrupted food structures) or low-fiber disrupted food structures. We used nasoenteric tubes to sample chyme from the intact distal ileum lumina of humans in the fasted state and every 60 min for 480 min postprandially. We demonstrate the highly dynamic, wide-ranging molecular environment of the ileum over time, with a substantial decrease in ileum bacterial numbers and bacterial metabolites after food intake. We also show that high-fiber diets, independent of food structure, increased PYY release compared with a low-fiber diet during 0 to 240 min postprandially. High-fiber diets also increased ileal stachyose, and a disrupted high-fiber diet increased certain ileal amino acids. Treatment of human ileal organoids with ileal fluids or an amino acid and stachyose mixture stimulated PYY expression in a similar profile to blood PYY concentrations, confirming the role of ileal metabolites in PYY release. Our study demonstrates the diet-induced changes over time in the metabolite environment of intact human ileum, which play a role in PYY release. Editor's summary: The digestion of different foods as they travel the length of the gastrointestinal tract is highly orchestrated, and the true complexity of this process is just beginning to be understood. Dagbasi et al. studied how fiber content and food structure affect nutrient metabolism and release of appetite-controlling peptides such as PYY in the human ileum. They found that high dietary fiber consumption, regardless of the extent of food processing, promoted ileal PYY (but not GLP-1) release in healthy volunteers. This PYY release was promoted by ileal metabolites, showing how what we eat may signal to the intestine to feed back on whole-body physiology. —Catherine Charneski [ABSTRACT FROM AUTHOR]

Published
2024
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7. Considerations for peripheral blood transport and storage during large-scale multicentre metabolome research.

Author

Alexander, James L., Wyatt, Nicola J., Camuzeaux, Stephane, Chekmeneva, Elena, Jimenez, Beatriz, Sands, Caroline J., Fuller, Hannah, Takis, Panteleimon, Ahmad, Tariq, Doyle, Jennifer A., Hart, Ailsa, Irving, Peter M., Kennedy, Nicholas A., Lees, Charlie W., Lindsay, James O., McIntyre, Rebecca E., Parkes, Miles, Prescott, Natalie J., Raine, Tim, and Satsangi, Jack

Subjects
SCHOLARSHIPS, PROTON magnetic resonance, SHOTGUN sequencing, FECAL occult blood tests
Published
2024
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9. Normal Cerebrospinal Fluid Pyridoxal 5′-Phosphate Level in a PNPO-Deficient Patient with Neonatal-Onset Epileptic Encephalopathy

Author

Levtova, Alina, Camuzeaux, Stephane, Laberge, Anne-Marie, Allard, Pierre, Brunel-Guitton, Catherine, Diadori, Paola, Rossignol, Elsa, Hyland, Keith, Clayton, Peter T., Mills, Philippa B., Mitchell, Grant A., Zschocke, Johannes, Editor-in-chief, Baumgartner, Matthias, editor, Morava, Eva, editor, Patterson, Marc, editor, Rahman, Shamima, editor, and Peters, Verena, editor

Published
2015
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10. Supplementary Table 1, Figures 1 - 3 from Serum CA19-9 Is Significantly Upregulated up to 2 Years before Diagnosis with Pancreatic Cancer: Implications for Early Disease Detection

Author

O'Brien, Darragh P., primary, Sandanayake, Neomal S., primary, Jenkinson, Claire, primary, Gentry-Maharaj, Aleksandra, primary, Apostolidou, Sophia, primary, Fourkala, Evangelia-Ourania, primary, Camuzeaux, Stephane, primary, Blyuss, Oleg, primary, Gunu, Richard, primary, Dawnay, Anne, primary, Zaikin, Alexey, primary, Smith, Ross C., primary, Jacobs, Ian J., primary, Menon, Usha, primary, Costello, Eithne, primary, Pereira, Stephen P., primary, and Timms, John F., primary

Published
2023
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11. Commentary on “The road to reliable peptide assays is paved with good guidelines”

Author

Kowalka, Anna M., primary, Alexiadou, Kleopatra, additional, Cuenco, Joyceline, additional, Clarke, Rosemary E., additional, Camuzeaux, Stephane, additional, Minnion, James, additional, Williams, Emma L., additional, Bech, Paul, additional, Purkayastha, Sanjay, additional, Ahmed, Ahmed R., additional, Takats, Zoltan, additional, Khoo, Bernard, additional, Whitwell, Harry J., additional, Romero, Maria G., additional, Bloom, Stephen R., additional, Lewis, Matthew R., additional, and Tan, Tricia M.‐M., additional

Published
2023
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12. Considerations for peripheral blood transport and storage during large-scale multicentre metabolome research

Author

Alexander, James L, primary, Wyatt, Nicola J, additional, Camuzeaux, Stephane, additional, Chekmeneva, Elena, additional, Jimenez, Beatriz, additional, Sands, Caroline J, additional, Fuller, Hannah, additional, Takis, Panteleimon, additional, Ahmad, Tariq, additional, Doyle, Jennifer A, additional, Hart, Ailsa, additional, Irving, Peter M, additional, Kennedy, Nicholas A, additional, Lees, Charlie W, additional, Lindsay, James O, additional, McIntyre, Rebecca E, additional, Parkes, Miles, additional, Prescott, Natalie J, additional, Raine, Tim, additional, Satsangi, Jack, additional, Speight, Richard Alexander, additional, Jostins-Dean, Luke, additional, Powell, Nick, additional, Marchesi, Julian R, additional, Stewart, Christopher J, additional, and Lamb, Christopher A, additional

Published
2023
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13. Multiprobabilistic Venn Predictors with Logistic Regression

Author

Nouretdinov, Ilia, Devetyarov, Dmitry, Burford, Brian, Camuzeaux, Stephane, Gentry-Maharaj, Aleksandra, Tiss, Ali, Smith, Celia, Luo, Zhiyuan, Chervonenkis, Alexey, Hallett, Rachel, Vovk, Volodya, Waterfield, Mike, Cramer, Rainer, Timms, John F., Jacobs, Ian, Menon, Usha, Gammerman, Alexander, Iliadis, Lazaros, editor, Maglogiannis, Ilias, editor, Papadopoulos, Harris, editor, Karatzas, Kostas, editor, and Sioutas, Spyros, editor

Published
2012
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14. The postprandial secretion of peptide YY 1‐36 and 3‐36 in obesity is differentially increased after gastric bypass versus sleeve gastrectomy

Author

Kowalka, Anna M., primary, Alexiadou, Kleopatra, additional, Cuenco, Joyceline, additional, Clarke, Rosemary E., additional, Minnion, James, additional, Williams, Emma L., additional, Bech, Paul, additional, Purkayastha, Sanjay, additional, Ahmed, Ahmed R., additional, Takats, Zoltan, additional, Whitwell, Harry J., additional, Romero, Maria Gomez, additional, Bloom, Stephen R., additional, Camuzeaux, Stephane, additional, Lewis, Matthew R., additional, Khoo, Bernard, additional, and Tan, Tricia M.‐M., additional

Published
2022
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15. The postprandial secretion of peptide YY1‐36 and 3‐36 in obesity is differentially increased after gastric bypass versus sleeve gastrectomy.

Author

Kowalka, Anna M., Alexiadou, Kleopatra, Cuenco, Joyceline, Clarke, Rosemary E., Minnion, James, Williams, Emma L., Bech, Paul, Purkayastha, Sanjay, Ahmed, Ahmed R., Takats, Zoltan, Whitwell, Harry J., Romero, Maria Gomez, Bloom, Stephen R., Camuzeaux, Stephane, Lewis, Matthew R., Khoo, Bernard, and Tan, Tricia M.‐M.

Subjects
GASTRIC bypass, SLEEVE gastrectomy, PEPTIDES, LIQUID chromatography-mass spectrometry, SECRETION, APPETITE stimulants
Abstract

Objectives: Peptide tyrosine tyrosine (PYY) exists as two species, PYY1‐36 and PYY3‐36, with distinct effects on insulin secretion and appetite regulation. The detailed effects of bariatric surgery on PYY1‐36 and PYY3‐36 secretion are not known as previous studies have used nonspecific immunoassays to measure total PYY. Our objective was to characterize the effect of sleeve gastrectomy (SG) and Roux‐en‐Y gastric bypass (RYGB) on fasting and postprandial PYY1‐36 and PYY3‐36 secretion using a newly developed liquid chromatography‐tandem mass spectrometry (LC‐MS/MS) assay. Design and Subjects: Observational study in 10 healthy nonobese volunteers and 30 participants with obesity who underwent RYGB (n = 24) or SG (n = 6) at the Imperial Weight Centre [NCT01945840]. Participants were studied using a standardized mixed meal test (MMT) before and 1 year after surgery. The outcome measures were PYY1‐36 and PYY3‐36 concentrations. Results: Presurgery, the fasting and postprandial levels of PYY1‐36 and PYY3‐36 were low, with minimal responses to the MMT, and these did not differ from healthy nonobese volunteers. The postprandial secretion of both PYY1‐36 and PYY3‐36 at 1 year was amplified after RYGB, but not SG, with the response being significantly higher in RYGB compared with SG. Conclusions: There appears to be no difference in PYY secretion between nonobese and obese volunteers at baseline. At 1 year after surgery, RYGB, but not SG, is associated with increased postprandial secretion of PYY1‐36 and PYY3‐36, which may account for long‐term differences in efficacy and adverse effects between the two types of surgery. [ABSTRACT FROM AUTHOR]

Published
2023
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16. Metabolome-wide association study on ABCA7 indicates a role of ceramide metabolism in Alzheimer’s disease

Author

Dehghan, Abbas, primary, Pinto, Rui Climaco, additional, Karaman, Ibrahim, additional, Huang, Jian, additional, Durainayagam, Brenan R., additional, Ghanbari, Mohsen, additional, Nazeer, Areesha, additional, Zhong, Qi, additional, Liggi, Sonia, additional, Whiley, Luke, additional, Mustafa, Rima, additional, Kivipelto, Miia, additional, Solomon, Alina, additional, Ngandu, Tiia, additional, Kanekiyo, Takahisa, additional, Aikawa, Tomonori, additional, Radulescu, Carola I., additional, Barnes, Samuel J., additional, Graça, Gonçalo, additional, Chekmeneva, Elena, additional, Camuzeaux, Stephane, additional, Lewis, Matthew R., additional, Kaluarachchi, Manuja R., additional, Ikram, M. Arfan, additional, Holmes, Elaine, additional, Tzoulaki, Ioanna, additional, Matthews, Paul M., additional, Griffin, Julian L., additional, and Elliott, Paul, additional

Published
2022
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17. B7-H3 Associates with IMPDH2 and Regulates Cancer Cell Survival.

Author

Alhamad, Salwa, Elmasry, Yassmin, Uwagboe, Isabel, Chekmeneva, Elena, Sands, Caroline, Cooper, Benjamin W., Camuzeaux, Stephane, Salam, Ash, and Parsons, Maddy

Subjects
ENZYME metabolism, PROGRAMMED cell death 1 receptors, DISEASE progression, HOMEOSTASIS, IMMUNE checkpoint proteins, LUNG tumors, CELL receptors, CELL survival, GENE expression, OXIDATIVE stress, CELL motility, CELLULAR signal transduction, RESEARCH funding, CELL lines, IMMUNOTHERAPY
Abstract

Simple Summary: Lung cancer is one of the most common cancers in the world, and despite improvements in treatment, it remains a significant health burden. B7-H3 is a member of the B7 immune checkpoint family of membrane proteins and shows increased expression in lung cancers; however, the functional consequences of this to cancer cell signalling remain unclear. Here, we show that B7-H3 forms a complex with the metabolic enzyme IMPDH2, and this protects cancer cells from oxidative stress and resulting apoptosis triggered by chemotherapy. The removal of B7-H3 from cancer cells in 2D results in sensitisation to chemotherapeutic stress and reduces cell viability in an IMPDH2-dependent manner. However, the loss of B7-H3 in 3D spheroid models results in enhanced IMPDH2-dependent spheroid growth, likely due to the weakening of cell–cell adhesion. These findings demonstrate that B7-H3 functionally contributes to metabolic signalling in lung cancer cells in the absence of immune cell interactions. Lung cancer is one of the most common cancers worldwide, and despite improvements in treatment regimens, patient prognosis remains poor. Lung adenocarcinomas develop from the lung epithelia and understanding how specific genetic and environmental factors lead to oncogenic transformation in these cells is of great importance to define the pathways that contribute to tumorigenesis. The recent rise in the use of immunotherapy to treat different cancers has prompted the exploration of immune modulators in tumour cells that may provide new targets to manipulate this process. Of these, the B7 family of cell surface receptors, which includes PD-1, is of particular interest due to its role in modulating immune cell responses within the tumour microenvironment. B7-H3 (CD276) is one family member that is upregulated in many cancer types and suggested to contribute to tumour–immune interactions. However, the function and ligand(s) for this receptor in normal lung epithelia and the mechanisms through which the overexpression of B7-H3 regulate cancer progression in the absence of immune cell interactions remain unclear. Here, we present evidence that B7-H3 is associated with one of the key rate-limiting metabolic enzymes IMPDH2, and the localisation of this complex is altered in human lung cancer cells that express high levels of B7-H3. Mechanistically, the IMPDH2:B7-H3 complex provides a protective role in cancer cells to escape oxidative stress triggered by chemotherapy, thus leading to cell survival. We further demonstrate that the loss of B7-H3 in cancer cells has no effect on growth or migration in 2D but promotes the expansion of 3D spheroids in an IMPDH2-dependent manner. These findings provide new insights into the B7-H3 function in the metabolic homeostasis of normal and transformed lung cancer cells, and whilst this molecule remains an interesting target for immunotherapy, these findings caution against the use of anti-B7-H3 therapies in certain clinical settings. [ABSTRACT FROM AUTHOR]

Published
2023
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18. Multiprobabilistic prediction in early medical diagnoses

Author

Nouretdinov, Ilia, Devetyarov, Dmitry, Vovk, Volodya, Burford, Brian, Camuzeaux, Stephane, Gentry-Maharaj, Aleksandra, Tiss, Ali, Smith, Celia, Luo, Zhiyuan, Chervonenkis, Alexey, Hallett, Rachel, Waterfield, Mike, Cramer, Rainer, Timms, John F., Jacobs, Ian, Menon, Usha, and Gammerman, Alex

Published
2015
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20. Metabolome-wide association study on ABCA7 indicates a role of ceramide metabolism in Alzheimer's disease

Author

Dehghan, Abbas, Pinto, Rui Climaco, Karaman, Ibrahim, Huang, Jian, Durainayagam, Brenan R., Ghanbari, Mohsen, Nazeer, Areesha, Zhong, Qi, Liggi, Sonia, Whiley, Luke, Mustafa, Rima, Kivipelto, Miia, Solomon, Alina, Ngandu, Tiia, Kanekiyo, Takahisa, Aikawa, Tomonori, Radulescu, Carola I., Barnes, Samuel J., Graça, Gonçalo, Chekmeneva, Elena, Camuzeaux, Stephane, Lewis, Matthew R., Kaluarachchi, Manuja R., Ikram, M. Arfan, Holmes, Elaine, Tzoulaki, Ioanna, Matthews, Paul M., Griffin, Julian L., Elliott, Paul, Dehghan, Abbas, Pinto, Rui Climaco, Karaman, Ibrahim, Huang, Jian, Durainayagam, Brenan R., Ghanbari, Mohsen, Nazeer, Areesha, Zhong, Qi, Liggi, Sonia, Whiley, Luke, Mustafa, Rima, Kivipelto, Miia, Solomon, Alina, Ngandu, Tiia, Kanekiyo, Takahisa, Aikawa, Tomonori, Radulescu, Carola I., Barnes, Samuel J., Graça, Gonçalo, Chekmeneva, Elena, Camuzeaux, Stephane, Lewis, Matthew R., Kaluarachchi, Manuja R., Ikram, M. Arfan, Holmes, Elaine, Tzoulaki, Ioanna, Matthews, Paul M., Griffin, Julian L., and Elliott, Paul

Abstract

Genome-wide association studies (GWASs) have identified genetic loci associated with the risk of Alzheimer's disease (AD), but the molecular mechanisms by which they confer risk are largely unknown. We conducted a metabolome-wide association study (MWAS) of AD-associated loci from GWASs using untargeted metabolic profiling (metabolomics) by ultraperformance liquid chromatography-mass spectrometry (UPLC-MS). We identified an association of lactosylceramides (LacCer) with AD-related single-nucleotide polymorphisms (SNPs) in ABCA7 (P = 5.0 × 10-5 to 1.3 × 10-44). We showed that plasma LacCer concentrations are associated with cognitive performance and genetically modified levels of LacCer are associated with AD risk. We then showed that concentrations of sphingomyelins, ceramides, and hexosylceramides were altered in brain tissue from Abca7 knockout mice, compared with wild type (WT) (P = 0.049-1.4 × 10-5), but not in a mouse model of amyloidosis. Furthermore, activation of microglia increases intracellular concentrations of hexosylceramides in part through induction in the expression of sphingosine kinase, an enzyme with a high control coefficient for sphingolipid and ceramide synthesis. Our work suggests that the risk for AD arising from functional variations in ABCA7 is mediated at least in part through ceramides. Modulation of their metabolism or downstream signaling may offer new therapeutic opportunities for AD.

Published
2022

21. An Open Platform for Large Scale LC-MS-Based Metabolomics

Author

Lewis, Matthew, primary, Chekmeneva, Elena, additional, Camuzeaux, Stephane, additional, Sands, Caroline, additional, Yuen, Ada, additional, David, Mark, additional, Salam, Ash, additional, Chappell, Katie, additional, Cooper, Benjamin, additional, Haggart, Gordon, additional, Maslen, Lynn, additional, Gómez-Romero, María, additional, Horneffer-van der Sluis, Verena, additional, Correia, Gonçalo, additional, and Takats, Zoltan, additional

Published
2022
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22. Improved Quantitation of Short-Chain Carboxylic Acids in Human Biofluids Using 3-Nitrophenylhydrazine Derivatization and Liquid Chromatography with Tandem Mass Spectrometry (LC-MS/MS)

Author

Valdivia-Garcia, Maria, primary, Chappell, Katie, additional, Camuzeaux, Stephane, additional, Olmo-García, Lucía, additional, Horneffer-van der Sluis, Verena, additional, Radhakrishnan, Shiva T., additional, Stephens, Hannah, additional, Bouri, Sonia, additional, de Campos Braz, Lucia M., additional, Williams, Horace R.T., additional, Lewis, Mathew R., additional, Frost, Gary, additional, and Li, Jia V., additional

Published
2022
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24. Epilepsy due to PNPO mutations: genotype, environment and treatment affect presentation and outcome

Author

Mills, Philippa B., Camuzeaux, Stephane S.M., Footitt, Emma J., Mills, Kevin A., Gissen, Paul, Fisher, Laura, Das, Krishna B., Varadkar, Sophia M., Zuberi, Sameer, McWilliam, Robert, Stödberg, Tommy, Plecko, Barbara, Baumgartner, Matthias R., Maier, Oliver, Calvert, Sophie, Riney, Kate, Wolf, Nicole I., Livingston, John H., Bala, Pronab, Morel, Chantal F., Feillet, François, Raimondi, Francesco, Del Giudice, Ennio, Chong, W. Kling, Pitt, Matthew, and Clayton, Peter T.

Published
2014
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26. Metabolome-wide association study on ABCA7 demonstrates a role for ceramide metabolism in impaired cognitive performance and Alzheimer’s disease

Author

Dehghan, Abbas, primary, Pinto, Rui, additional, Karaman, Ibrahim, additional, Huang, Jian, additional, Durainayagam, Brenan R, additional, Liggi, Sonia, additional, Whiley, Luke, additional, Mustafa, Rima, additional, Kivipelto, Miia, additional, Solomon, Alina, additional, Ngandu, Tiia, additional, Kanekiyo, Takahisa, additional, Aikawa, Tomonori, additional, Chekmeneva, Elena, additional, Camuzeaux, Stephane, additional, Lewis, Matthew R., additional, Kaluarachchi, Manuja R, additional, Ghanbari, Mohsen, additional, Ikram, M Arfan, additional, Holmes, Elaine, additional, Tzoulaki, Ioanna, additional, Matthews, Paul M., additional, Griffin, Julian L., additional, and Elliott, Paul, additional

Published
2021
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28. Plasma lipid profiles discriminate bacterial from viral infection in febrile children

Author

Wang, Xinzhu, Nijman, Ruud, Camuzeaux, Stephane, Sands, Caroline, Jackson, Heather, Kaforou, Myrsini, Emonts, Marieke, Herberg, Jethro A, Maconochie, Ian, Carrol, Enitan D, Paulus, Stephane C, Zenz, Werner, Van der Flier, Michiel, de Groot, Ronald, Martinon-Torres, Federico, Schlapbach, Luregn J, Pollard, Andrew J, Fink, Colin, Kuijpers, Taco T, Anderson, Suzanne, Lewis, Matthew R, Levin, Michael, McClure, Myra, Gormley, Stuart, Hamilton, Shea, Hourmat, Bernardo, Hoggart, Clive, Sancho-Shimizu, Vanessa, Wright, Victoria, Abdulla, Amina, Agapow, Paul, Bartlett, Maeve, Bellos, Evangelos, Eleftherohorinou, Hariklia, Galassini, Rachel, Inwald, David, Mashbat, Meg, Menikou, Stefanie, Mustafa, Sobia, Nadel, Simon, Rahman, Rahmeen, Thakker, Clare, Coin, Lachlan MJ, Bokhand, S, Power, Sue, Barham, Heather, Pathan, N, Ridout, Jenna, White, Deborah, Thurston, Sarah, Faust, S, Patel, S, McCorkell, Jenni, Davies, P, Crate, Lindsey, Navarra, Helen, Carter, Stephanie, Ramaiah, R, Patel, Rekha, Tuffrey, Catherine, Gribbin, Andrew, McCready, Sharon, Peters, Mark, Hardy, Katie, Standing, Fran, O'Neill, Lauren, Abelake, Eugenia, Deep, Akash, Nsirim, Eniola, Willis, Louise, Young, Zoe, Royad, C, White, Sonia, Fortune, PM, Hudnott, Phil, Alvez Gonzalez, Fernando, Barral-Arca, Ruth, Cebey-Lopez, Miriam, Jose Curras-Tuala, Maria, Garcia, Natalia, Garcia Vicente, Luisa, Gomez-Carballa, Alberto, Gomez Rial, Jose, Grela Beiroa, Andrea, Justicia Grande, Antonio, Leborans Iglesias, Pilar, Martinez Santos, Alba Elena, MartinonTorres, Nazareth, Martinon Sanchez, Jose Maria, Morillo Gutierrez, Beatriz, Mosquera Perez, Belen, Obando Pacheco, Pablo, Pardo-Seco, Jacobo, Pischedda, Sara, RiveroCalle, Irene, Rodriguez-Tenreiro, Carmen, Redondo-Collazo, Lorenzo, Salas Ellacuriagal, Antonio, Seren Fernandez, Sonia, Porto Silva, Maria del Sol, Vega, Ana, Vilanova Trillo, Lucia, Salas, Antonio, Beatriz Reyes, Susana, Leon Leon, Maria Cruz, Navarro Mingorance, Alvaro, Gabaldo Barrios, Xavier, Onate Vergara, Eider, Concha Torre, Andres, Vivanco, Ana, Fernandez, Reyes, Gimenez Sanchez, Francisco, Sanchez Forte, Miguel, Rojo, Pablo, Ruiz Contreras, J, Palacios, Alba, Epalza Ibarrondo, Cristina, Fernandez Cooke, Elizabeth, Navarro, Marisa, Alvarez Alvarez, Cristina, Jose Lozano, Maria, Carreras, Eduardo, Brio Sanagustin, Sonia, Neth, Olaf, Martinez Padilla, Ma del Carmen, Prieto Tato, Luis Manuel, Guillen, Sara, Fernandez Silveira, Laura, Moreno, David, van Furth, AM Tutu, Boeddha, NP, Driessen, GJA, Emonts, M, Hazelzet, JA, Pajkrt, D, Sanders, EAM, van de Beek, D, van der Ende, A, Philipsen, HLA, Adeel, AOA, Breukels, MA, Brinkman, DMC, de Korte, CCMM, de Vries, E, de Waal, WJ, Dekkers, R, Dings-Lammertink, A, Doedens, RA, Donker, AE, Dousma, M, Faber, TE, Gerrits, GPJM, Gerver, JAM, Heidema, J, Homan-van der Veen, J, Jacobs, MAM, Jansen, NJG, Kawczynski, P, Klucovska, K, Kneyber, MCJ, Koopman-Keemink, Y, Langenhorst, VJ, Leusink, J, Loza, BF, Merth, IT, Miedema, CJ, Neeleman, C, Noordzij, JG, Obihara, CC, van Overbeek-van Gils, ALT, Poortman, GH, Potgieter, ST, Potjewijd, J, Rosias, PPR, Sprong, T, ten Tussher, GW, Thio, BJ, Tramper-Stranders, GA, van Deuren, M, van der Meer, H, van Kuppevelt, AJM, van Wermeskerken, AM, Verwijs, WA, Wolfs, TFW, Agyeman, Philipp, Aebi, Christoph, Berger, Christoph, Giannoni, Eric, Stocker, Martin, Posfay-Barbe, Klara M, Heininger, Ulrich, Bernhard-Stirnemann, Sara, Niederer-Loher, Anita, Kahlert, Christian, Hasters, Paul, Relly, Christa, Baer, Walter, Frederick, Hannah, Jennings, Rebecca, Johnston, Joanne, Kenwright, Rhian, Pinnock, Elli, Agbeko, Rachel, Secka, Fatou, Bojang, Kalifa, Sarr, Isatou, Kebbeh, Ngange, Sey, Gibbi, Momodou, Saidy Khan, Cole, Fatoumata, Thomas, Gilleh, Antonio, Martin, Klobassa, Daniela S, Binder, Alexander, Schweintzger, Nina A, Sagmeister, Manfred, Baumgart, Hinrich, Baumgartner, Markus, Behrends, Uta, Biebl, Ariane, Birnbacher, Robert, Blanke, Jan-Gerd, Boelke, Carsten, Breuling, Kai, Brunner, Juergen, Buller, Maria, Dahlem, Peter, Dietrich, Beate, Eber, Ernst, Elias, Johannes, Emhofer, Josef, Etschmaier, Rosa, Farr, Sebastian, Girtler, Ylenia, Grigorow, Irina, Heimann, Konrad, Ihm, Ulrike, Jaros, Zdenek, Kalhoff, Hermann, Kaulfersch, Wilhelm, Kemen, Christoph, Klocker, Nina, Koester, Bernhard, Kohlmaier, Benno, Komini, Eleni, Kramer, Lydia, Neubert, Antje, Ortner, Daniel, Pescollderungg, Lydia, Pfurtscheller, Klaus, Reiter, Karl, Ristic, Goran, Roedl, Siegfried, Sellner, Andrea, Sonnleitner, Astrid, Sperl, Matthias, Stelzl, Wolfgang, Till, Holger, Trobisch, Andreas, Vierzig, Anne, Vogel, Ulrich, Weingarten, Christina, Welke, Stefanie, Wimmer, Andreas, Wintergerst, Uwe, Wueller, Daniel, Zaunschirm, Andrew, Ziuraite, Ieva, Zukovskaja, Veslava, Consortium, EUCLIDS, Sands, Caroline [0000-0002-3749-7941], Jackson, Heather [0000-0002-1054-9983], Kaforou, Myrsini [0000-0001-9878-4007], Paulus, Stephane C [0000-0002-0703-9114], Martinon-Torres, Federico [0000-0002-9023-581X], Apollo - University of Cambridge Repository, Pediatric surgery, AII - Infectious diseases, Internal medicine, Pathology, Psychiatry, Amsterdam Reproduction & Development (AR&D), Posfay Barbe, Klara, European Commission, National Institute for Health Research (UK), NIHR Biomedical Research Centre (UK), Medical Research Council (UK), Wellcome Trust, Pediatrics, Public Health, Paediatric Infectious Diseases / Rheumatology / Immunology, Amsterdam Neuroscience - Neuroinfection & -inflammation, Neurology, Medical Microbiology and Infection Prevention, and Critical care, Anesthesiology, Peri-operative and Emergency medicine (CAPE)

Subjects
Male, 0301 basic medicine, ACCURACY, Vascular damage Radboud Institute for Health Sciences [Radboudumc 16], MENINGITIS, Antibiotics, lnfectious Diseases and Global Health Radboud Institute for Molecular Life Sciences [Radboudumc 4], MathematicsofComputing_GENERAL, lcsh:Medicine, 0601 Biochemistry and Cell Biology, chemistry.chemical_compound, 0302 clinical medicine, ComputingMethodologies_SYMBOLICANDALGEBRAICMANIPULATION, Blood plasma, 030212 general & internal medicine, Child, lcsh:Science, ddc:618, Multidisciplinary, music.instrument, TheoryofComputation_GENERAL, Bacterial Infections, Lipidome, ddc, Sphingomyelins, 3. Good health, Cholesterol, Virus Diseases, Child, Preschool, Female, Adolescent, medicine.drug_class, Bilirubin, Inositol Phosphates, 0299 Other Physical Sciences, 610 Medicine & health, DIAGNOSIS, Fever of Unknown Origin, Article, Virus, Diagnosis, Differential, 03 medical and health sciences, Lactosylceramide, Journal Article, medicine, Metabolomics, Humans, General, music, business.industry, lcsh:R, Infant, Lysophosphatidylcholines, Diagnostic markers, Omics, EUCLIDS consortium, 030104 developmental biology, chemistry, Immunology, lcsh:Q, Software_PROGRAMMINGLANGUAGES, business, ddc:600, Biomarkers
Abstract

EUCLIDS consortium., Fever is the most common reason that children present to Emergency Departments. Clinical signs and symptoms suggestive of bacterial infection are often non-specific, and there is no definitive test for the accurate diagnosis of infection. The ‘omics’ approaches to identifying biomarkers from the host-response to bacterial infection are promising. In this study, lipidomic analysis was carried out with plasma samples obtained from febrile children with confirmed bacterial infection (n = 20) and confirmed viral infection (n = 20). We show for the first time that bacterial and viral infection produces distinct profile in the host lipidome. Some species of glycerophosphoinositol, sphingomyelin, lysophosphatidylcholine and cholesterol sulfate were higher in the confirmed virus infected group, while some species of fatty acids, glycerophosphocholine, glycerophosphoserine, lactosylceramide and bilirubin were lower in the confirmed virus infected group when compared with confirmed bacterial infected group. A combination of three lipids achieved an area under the receiver operating characteristic (ROC) curve of 0.911 (95% CI 0.81 to 0.98). This pilot study demonstrates the potential of metabolic biomarkers to assist clinicians in distinguishing bacterial from viral infection in febrile children, to facilitate effective clinical management and to the limit inappropriate use of antibiotics., This work was partially supported by the European Seventh Framework Programme for Research and Technological Development (FP7) under EUCLIDS Grant Agreement no. 279185. ICED: The Research was supported by the National Institute for Health Research Biomedical Research Centre based at Imperial College. This work was further supported by the Medical Research Council and National Institute for Health Research [grant number MC_PC_12025] through funding for the MRC-NIHR National Phenome Centre, infrastructure support was provided by the National Institute for Health Research (NIHR) Biomedical Research Centre (BRC) at Imperial NHS Healthcare Trust. MK acknowledges funding from the Wellcome Trust (Sir Henry Wellcome Fellowship grant 206508/Z/17/Z).

Published
2019
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29. Plasma lipid profiles discriminate bacterial from viral infection in febrile children

Author

European Commission, National Institute for Health Research (UK), NIHR Biomedical Research Centre (UK), Medical Research Council (UK), Wellcome Trust, Wang, Xinzhu, Nijman, Ruud, Camuzeaux, Stephane, Sands, Caroline, Jackson, Heather, Kaforou, Myrsini, Emonts, Marieke, Herberg, Jethro A., Maconochie, Ian, Carrol, Enitan D., Paulus, Stephane C., Zenz, Werner, Van der Flier, Michiel, De Groot, Ronald, Martinon-Torres, Federico, Schlapbach, Luregn J., Pollard, Andrew J., Fink, Colin, Kuijpers, Taco W., Anderson, Suzanne, Lewis, Matthew R., Levin, Michael, McClure, Myra, European Commission, National Institute for Health Research (UK), NIHR Biomedical Research Centre (UK), Medical Research Council (UK), Wellcome Trust, Wang, Xinzhu, Nijman, Ruud, Camuzeaux, Stephane, Sands, Caroline, Jackson, Heather, Kaforou, Myrsini, Emonts, Marieke, Herberg, Jethro A., Maconochie, Ian, Carrol, Enitan D., Paulus, Stephane C., Zenz, Werner, Van der Flier, Michiel, De Groot, Ronald, Martinon-Torres, Federico, Schlapbach, Luregn J., Pollard, Andrew J., Fink, Colin, Kuijpers, Taco W., Anderson, Suzanne, Lewis, Matthew R., Levin, Michael, and McClure, Myra

Abstract

Fever is the most common reason that children present to Emergency Departments. Clinical signs and symptoms suggestive of bacterial infection are often non-specific, and there is no definitive test for the accurate diagnosis of infection. The ‘omics’ approaches to identifying biomarkers from the host-response to bacterial infection are promising. In this study, lipidomic analysis was carried out with plasma samples obtained from febrile children with confirmed bacterial infection (n = 20) and confirmed viral infection (n = 20). We show for the first time that bacterial and viral infection produces distinct profile in the host lipidome. Some species of glycerophosphoinositol, sphingomyelin, lysophosphatidylcholine and cholesterol sulfate were higher in the confirmed virus infected group, while some species of fatty acids, glycerophosphocholine, glycerophosphoserine, lactosylceramide and bilirubin were lower in the confirmed virus infected group when compared with confirmed bacterial infected group. A combination of three lipids achieved an area under the receiver operating characteristic (ROC) curve of 0.911 (95% CI 0.81 to 0.98). This pilot study demonstrates the potential of metabolic biomarkers to assist clinicians in distinguishing bacterial from viral infection in febrile children, to facilitate effective clinical management and to the limit inappropriate use of antibiotics.

Published
2019

30. Kisspeptin stimulates insulin secretion and modulates serum metabolites in humans

Author

Izzi, Engbeaya Chioma, primary, Comninos, Alexander N, additional, Clarke, Sophie A, additional, Jomard, Anne, additional, Yang, Lisa, additional, Jones, Sophie, additional, Abbara, Ali, additional, Narayanaswamy, Shakunthala, additional, Chia, Eng Pei, additional, Papadopoulou, Deborah, additional, Prague, Julia K, additional, Bech, Paul, additional, Godsland, Ian F, additional, Bassett, Paul, additional, Sands, Caroline, additional, Camuzeaux, Stephane, additional, Gomez, Romero Maria, additional, Pearce, Jake TM, additional, Lewis, Matthew R, additional, Holmes, Elaine, additional, Nicholson, Jeremy, additional, Tan, Tricia, additional, Ratnasabapathy, Risheka, additional, Hu, Ming, additional, Carrat, Gaelle, additional, Piemonti, Lorenzo, additional, Bugliani, Marco, additional, Marchetti, Piero, additional, Johnson, Paul R, additional, Hughes, Stephen J, additional, James, Shapiro AM, additional, Rutter, Guy A, additional, and Dhillo, Waljit S, additional

Published
2018
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31. The effects of kisspeptin on β-cell function, serum metabolites and appetite in humans

Author

Izzi-Engbeaya, Chioma, primary, Comninos, Alexander N., additional, Clarke, Sophie A., additional, Jomard, Anne, additional, Yang, Lisa, additional, Jones, Sophie, additional, Abbara, Ali, additional, Narayanaswamy, Shakunthala, additional, Eng, Pei Chia, additional, Papadopoulou, Deborah, additional, Prague, Julia K., additional, Bech, Paul, additional, Godsland, Ian F., additional, Bassett, Paul, additional, Sands, Caroline, additional, Camuzeaux, Stephane, additional, Gomez-Romero, Maria, additional, Pearce, Jake T. M., additional, Lewis, Matthew R., additional, Holmes, Elaine, additional, Nicholson, Jeremy K., additional, Tan, Tricia, additional, Ratnasabapathy, Risheka, additional, Hu, Ming, additional, Carrat, Gaelle, additional, Piemonti, Lorenzo, additional, Bugliani, Marco, additional, Marchetti, Piero, additional, Johnson, Paul R., additional, Hughes, Stephen J., additional, James Shapiro, A. M., additional, Rutter, Guy A., additional, and Dhillo, Waljit S., additional

Published
2018
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32. Multiplex High-Throughput Targeted Proteomic Assay To Identify Induced Pluripotent Stem Cells

Author

Baud, Anna, primary, Wessely, Frank, additional, Mazzacuva, Francesca, additional, McCormick, James, additional, Camuzeaux, Stephane, additional, Heywood, Wendy E., additional, Little, Daniel, additional, Vowles, Jane, additional, Tuefferd, Marianne, additional, Mosaku, Olukunbi, additional, Lako, Majlinda, additional, Armstrong, Lyle, additional, Webber, Caleb, additional, Cader, M. Zameel, additional, Peeters, Pieter, additional, Gissen, Paul, additional, Cowley, Sally A., additional, and Mills, Kevin, additional

Published
2017
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33. Epilepsy due to PNPO mutations: genotype, environment and treatment affect presentation and outcome

Author

Mills, Philippa B., Camuzeaux, Stephane S.M., Footitt, Emma J., Mills, Kevin A., Gissen, Paul, Fisher, Laura, Das, Krishna B., Varadkar, Sophia M., Zuberi, Sameer, McWilliam, Robert, Stödberg, Tommy, Plecko, Barbara, Baumgartner, Matthias R., Maier, Oliver, Calvert, Sophie, Riney, Kate, Wolf, Nicole I., Livingston, John H., Bala, Pronab, Morel, Chantal F., Feillet, François, Raimondi, Francesco, Del Giudice, Ennio, Chong, W. Kling, Pitt, Matthew, Clayton, Peter T., Mills, Philippa B., Camuzeaux, Stephane S.M., Footitt, Emma J., Mills, Kevin A., Gissen, Paul, Fisher, Laura, Das, Krishna B., Varadkar, Sophia M., Zuberi, Sameer, McWilliam, Robert, Stödberg, Tommy, Plecko, Barbara, Baumgartner, Matthias R., Maier, Oliver, Calvert, Sophie, Riney, Kate, Wolf, Nicole I., Livingston, John H., Bala, Pronab, Morel, Chantal F., Feillet, François, Raimondi, Francesco, Del Giudice, Ennio, Chong, W. Kling, Pitt, Matthew, and Clayton, Peter T.

Abstract

Mutations in PNPO are a known cause of neonatal onset seizures that are resistant to pyridoxine but responsive to pyridoxal phosphate (PLP). Mills etal. show that PNPO mutations can also cause neonatal onset seizures that respond to pyridoxine but worsen with PLP, as well as PLP-responsive infantile spasms

Published
2017

34. Identification of novel bile acids as biomarkers for the early diagnosis of Niemann-Pick C disease

Author

Mazzacuva, Francesca, primary, Mills, Philippa, additional, Mills, Kevin, additional, Camuzeaux, Stephane, additional, Gissen, Paul, additional, Nicoli, Elena-Raluca, additional, Wassif, Christopher, additional, te Vruchte, Danielle, additional, Porter, Forbes D., additional, Maekawa, Masamitsu, additional, Mano, Nariyasu, additional, Iida, Takashi, additional, Platt, Frances, additional, and Clayton, Peter T., additional

Published
2016
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35. Proteomic Discovery and Development of a Multiplexed Targeted MRM-LC-MS/MS Assay for Urine Biomarkers of Extracellular Matrix Disruption in Mucopolysaccharidoses I, II, and VI

Author

Heywood, Wendy E., primary, Camuzeaux, Stephane, additional, Doykov, Ivan, additional, Patel, Nina, additional, Preece, Rhian-Lauren, additional, Footitt, Emma, additional, Cleary, Maureen, additional, Clayton, Peter, additional, Grunewald, Stephanie, additional, Abulhoul, Lara, additional, Chakrapani, Anupam, additional, Sebire, Neil J., additional, Hindmarsh, Peter, additional, de Koning, Tom J., additional, Heales, Simon, additional, Burke, Derek, additional, Gissen, Paul, additional, and Mills, Kevin, additional

Published
2015
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36. Urine biomarker discovery using label free proteomics reveals novel markers for the monitoring of treatment for mucopolysaccharide disorders

Author

Heywood, Wendy E., primary, Camuzeaux, Stephane, additional, McCormick, James, additional, Clayton, Peter, additional, Grunewald, Stephanie S., additional, Cleary, Maureen, additional, Footitt, Emma, additional, Abulhoul, Lara, additional, Vellodi, Ashok, additional, Gissen, Paul, additional, Heales, Simon, additional, Burke, Derek, additional, and Mills, Kevin A., additional

Published
2015
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37. Serum CA19-9 Is Significantly Upregulated up to 2 Years before Diagnosis with Pancreatic Cancer: Implications for Early Disease Detection

Author

O'Brien, Darragh P., primary, Sandanayake, Neomal S., additional, Jenkinson, Claire, additional, Gentry-Maharaj, Aleksandra, additional, Apostolidou, Sophia, additional, Fourkala, Evangelia-Ourania, additional, Camuzeaux, Stephane, additional, Blyuss, Oleg, additional, Gunu, Richard, additional, Dawnay, Anne, additional, Zaikin, Alexey, additional, Smith, Ross C., additional, Jacobs, Ian J., additional, Menon, Usha, additional, Costello, Eithne, additional, Pereira, Stephen P., additional, and Timms, John F., additional

Published
2015
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38. Epilepsy due to PNPO mutations: genotype, environment and treatment affect presentation and outcome

Author

Mills, Philippa B, Camuzeaux, Stephane S M, Footitt, Emma J, Mills, Kevin A, Gissen, Paul, Fisher, Laura, Das, Krishna B, Varadkar, Sophia M, Zuberi, Sameer, McWilliam, Robert, Stödberg, Tommy, Plecko, Barbara, Baumgartner, Matthias R; https://orcid.org/0000-0002-9270-0826, Maier, Oliver, Calvert, Sophie, Riney, Kate, Wolf, Nicole I, Livingston, John H, Bala, Pronab, Morel, Chantal F, Feillet, François, Raimondi, Francesco, Del Giudice, Ennio, Chong, W Kling, Pitt, Matthew, Clayton, Peter T, Mills, Philippa B, Camuzeaux, Stephane S M, Footitt, Emma J, Mills, Kevin A, Gissen, Paul, Fisher, Laura, Das, Krishna B, Varadkar, Sophia M, Zuberi, Sameer, McWilliam, Robert, Stödberg, Tommy, Plecko, Barbara, Baumgartner, Matthias R; https://orcid.org/0000-0002-9270-0826, Maier, Oliver, Calvert, Sophie, Riney, Kate, Wolf, Nicole I, Livingston, John H, Bala, Pronab, Morel, Chantal F, Feillet, François, Raimondi, Francesco, Del Giudice, Ennio, Chong, W Kling, Pitt, Matthew, and Clayton, Peter T

Abstract

The first described patients with pyridox(am)ine 5'-phosphate oxidase deficiency all had neonatal onset seizures that did not respond to treatment with pyridoxine but responded to treatment with pyridoxal 5'-phosphate. Our data suggest, however, that the clinical spectrum of pyridox(am)ine 5'-phosphate oxidase deficiency is much broader than has been reported in the literature. Sequencing of the PNPO gene was undertaken for a cohort of 82 individuals who had shown a reduction in frequency and severity of seizures in response to pyridoxine or pyridoxal 5'-phosphate. Novel sequence changes were studied using a new cell-free expression system and a mass spectrometry-based assay for pyridoxamine phosphate oxidase. Three groups of patients with PNPO mutations that had reduced enzyme activity were identified: (i) patients with neonatal onset seizures responding to pyridoxal 5'-phosphate (n = 6); (ii) a patient with infantile spasms (onset 5 months) responsive to pyridoxal 5'-phosphate (n = 1); and (iii) patients with seizures starting under 3 months of age responding to pyridoxine (n = 8). Data suggest that certain genotypes (R225H/C and D33V) are more likely to result in seizures that to respond to treatment with pyridoxine. Other mutations seem to be associated with infertility, miscarriage and prematurity. However, the situation is clearly complex with the same combination of mutations being seen in patients who responded and did not respond to pyridoxine. It is possible that pyridoxine responsiveness in PNPO deficiency is affected by prematurity and age at the time of the therapeutic trial. Other additional factors that are likely to influence treatment response and outcome include riboflavin status and how well the foetus has been supplied with vitamin B6 by the mother. For some patients there was a worsening of symptoms on changing from pyridoxine to pyridoxal 5'-phosphate. Many of the mutations in PNPO affected residues involved in binding flavin mononucleotide or

Published
2014

39. Discovery of serum biomarkers of ovarian cancer using complementary proteomic profiling strategies

Author

Timms, John F., primary, Arslan-Low, Elif, additional, Kabir, Musarat, additional, Worthington, Jenny, additional, Camuzeaux, Stephane, additional, Sinclair, John, additional, Szaub, Joanna, additional, Afrough, Babak, additional, Podust, Vladimir N., additional, Fourkala, Evangelia-Ourania, additional, Cubizolles, Myriam, additional, Kronenberg, Florian, additional, Fung, Eric T., additional, Gentry-Maharaj, Aleksandra, additional, Menon, Usha, additional, and Jacobs, Ian, additional

Published
2014
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41. Multiprobabilistic prediction in early medical diagnoses

Author

Nouretdinov, Ilia, primary, Devetyarov, Dmitry, additional, Vovk, Volodya, additional, Burford, Brian, additional, Camuzeaux, Stephane, additional, Gentry-Maharaj, Aleksandra, additional, Tiss, Ali, additional, Smith, Celia, additional, Luo, Zhiyuan, additional, Chervonenkis, Alexey, additional, Hallett, Rachel, additional, Waterfield, Mike, additional, Cramer, Rainer, additional, Timms, John F., additional, Jacobs, Ian, additional, Menon, Usha, additional, and Gammerman, Alex, additional

Published
2013
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42. S1890 Characterisation of Serum Proteins in Biliary Tract Cancer, PSC and Iac Using 2-Dimensional Difference Gel Electrophoresis and Tandem Mass Spectrometry

Author

Sandanayake, Neomal S., primary, Sinclair, John, additional, Andreola, Fausto, additional, Chapman, Michael H., additional, Camuzeaux, Stephane, additional, Webster, George, additional, Norton, Ian D., additional, Smith, Ross, additional, Timms, John F., additional, and Pereira, Stephen P., additional

Published
2010
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44. T1394 Serum CEACAM1 in the Preclinical Diagnosis of Pancreatic Adenocarcinoma

Author

Sandanayake, Neomal S., primary, Andreola, Fausto, additional, Camuzeaux, Stephane, additional, Sinclair, John, additional, Chapman, Michael H., additional, Webster, George, additional, Gentry-Maharaj, Aleksandra, additional, Menon, Usha, additional, Jacobs, Ian, additional, Pereira, Stephen P., additional, and Timms, John F., additional

Published
2010
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45. Peptides Generated Ex Vivo from Serum Proteins by Tumor-Specific Exopeptidases Are Not Useful Biomarkers in Ovarian Cancer

Author

Timms, John F, primary, Cramer, Rainer, primary, Camuzeaux, Stephane, primary, Tiss, Ali, primary, Smith, Celia, primary, Burford, Brian, primary, Nouretdinov, Ilia, primary, Devetyarov, Dmitry, primary, Gentry-Maharaj, Aleksandra, primary, Ford, Jeremy, primary, Luo, Zhiyuan, primary, Gammerman, Alex, primary, Menon, Usha, primary, and Jacobs, Ian, primary

Published
2010
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46. Serum Peptide Profiling using MALDI Mass Spectrometry

Author

Tiss, Ali, primary, Smith, Celia, additional, Camuzeaux, Stephane, additional, Kabir, Musarat, additional, Gayther, Simon, additional, Menon, Usha, additional, Waterfield, Mike, additional, Timms, John, additional, Jacobs, Ian, additional, and Cramer, Rainer, additional

Published
2007
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47. Highly Accurate Detection of Ovarian Cancer Using CA125 but Limited Improvement With Serum Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry Profiling.

Author

Tiss, Ali, Timms, John F., Smith, Celia, Devetyarov, Dmitry, Gentry-Maharaj, Aleksandra, Camuzeaux, Stephane, Burford, Brian, Nouretdinov, Ilia, Ford, Jeremy, Luo, Zhiyuan, Jacobs, Ian, Menon, Usha, Gammerman, Alex, and Cramer, Rainer

Abstract

Our objective was to test the performance of CA125 in classifying serum samples from a cohort of malignant and benign ovarian cancers and age-matched healthy controls and to assess whether combining information from matrix-assisted laser desorption/ionization (MALDI) time-of-flight profiling could improve diagnostic performance.Serum samples from women with ovarian neoplasms and healthy volunteers were subjected to CA125 assay and MALDI time-of-flight mass spectrometry (MS) profiling. Models were built from training data sets using discriminatory MALDI MS peaks in combination with CA125 values and tested their ability to classify blinded test samples. These were compared with models using CA125 threshold levels from 193 patients with ovarian cancer, 290 with benign neoplasm, and 2236 postmenopausal healthy controls.Using a CA125 cutoff of 30 U/mL, an overall sensitivity of 94.8% (96.6% specificity) was obtained when comparing malignancies versus healthy postmenopausal controls, whereas a cutoff of 65 U/mL provided a sensitivity of 83.9% (99.6% specificity). High classification accuracies were obtained for early-stage cancers (93.5% sensitivity). Reasons for high accuracies include recruitment bias, restriction to postmenopausal women, and inclusion of only primary invasive epithelial ovarian cancer cases. The combination of MS profiling information with CA125 did not significantly improve the specificity/accuracy compared with classifications on the basis of CA125 alone.We report unexpectedly good performance of serum CA125 using threshold classification in discriminating healthy controls and women with benign masses from those with invasive ovarian cancer. This highlights the dependence of diagnostic tests on the characteristics of the study population and the crucial need for authors to provide sufficient relevant details to allow comparison. Our study also shows that MS profiling information adds little to diagnostic accuracy. This finding is in contrast with other reports and shows the limitations of serum MS profiling for biomarker discovery and as a diagnostic tool. [ABSTRACT FROM AUTHOR]

Published
2010
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48. Plasma lipid profiles discriminate bacterial from viral infection in febrile children

Author

Wang, Xinzhu, Nijman, Ruud, Camuzeaux, Stephane, Sands, Caroline, Jackson, Heather, Kaforou, Myrsini, Emonts, Marieke, Herberg, Jethro A, Maconochie, Ian, Carrol, Enitan D, Paulus, Stephane C, Zenz, Werner, Van Der Flier, Michiel, De Groot, Ronald, Martinon-Torres, Federico, Schlapbach, Luregn J, Pollard, Andrew J, Fink, Colin, Kuijpers, Taco T, Anderson, Suzanne, Lewis, Matthew R, Levin, Michael, McClure, Myra, and EUCLIDS Consortium

Subjects
Male, Adolescent, Inositol Phosphates, Infant, Lysophosphatidylcholines, Bacterial Infections, Fever of Unknown Origin, 3. Good health, Sphingomyelins, Diagnosis, Differential, Cholesterol, Virus Diseases, Child, Preschool, Humans, Female, Child, Biomarkers
Abstract

Funder: NIHR Imperial BRC, Fever is the most common reason that children present to Emergency Departments. Clinical signs and symptoms suggestive of bacterial infection are often non-specific, and there is no definitive test for the accurate diagnosis of infection. The 'omics' approaches to identifying biomarkers from the host-response to bacterial infection are promising. In this study, lipidomic analysis was carried out with plasma samples obtained from febrile children with confirmed bacterial infection (n = 20) and confirmed viral infection (n = 20). We show for the first time that bacterial and viral infection produces distinct profile in the host lipidome. Some species of glycerophosphoinositol, sphingomyelin, lysophosphatidylcholine and cholesterol sulfate were higher in the confirmed virus infected group, while some species of fatty acids, glycerophosphocholine, glycerophosphoserine, lactosylceramide and bilirubin were lower in the confirmed virus infected group when compared with confirmed bacterial infected group. A combination of three lipids achieved an area under the receiver operating characteristic (ROC) curve of 0.911 (95% CI 0.81 to 0.98). This pilot study demonstrates the potential of metabolic biomarkers to assist clinicians in distinguishing bacterial from viral infection in febrile children, to facilitate effective clinical management and to the limit inappropriate use of antibiotics.

49. Plasma lipid profiles discriminate bacterial from viral infection in febrile children

Author

Wang, Xinzhu, Nijman, Ruud, Camuzeaux, Stephane, Sands, Caroline, Jackson, Heather, Kaforou, Myrsini, Emonts, Marieke, Herberg, Jethro A, Maconochie, Ian, Carrol, Enitan D, Paulus, Stephane C, Zenz, Werner, Van Der Flier, Michiel, De Groot, Ronald, Martinon-Torres, Federico, Schlapbach, Luregn J., Pollard, Andrew J, Fink, Colin, Kuijpers, Taco T, Anderson, Suzanne, Lewis, Matthew R, Levin, Michael, and McClure, Myra

Subjects
610 Medicine & health, 3. Good health
Abstract

Fever is the most common reason that children present to Emergency Departments. Clinical signs and symptoms suggestive of bacterial infection are often non-specific, and there is no definitive test for the accurate diagnosis of infection. The 'omics' approaches to identifying biomarkers from the host-response to bacterial infection are promising. In this study, lipidomic analysis was carried out with plasma samples obtained from febrile children with confirmed bacterial infection (n = 20) and confirmed viral infection (n = 20). We show for the first time that bacterial and viral infection produces distinct profile in the host lipidome. Some species of glycerophosphoinositol, sphingomyelin, lysophosphatidylcholine and cholesterol sulfate were higher in the confirmed virus infected group, while some species of fatty acids, glycerophosphocholine, glycerophosphoserine, lactosylceramide and bilirubin were lower in the confirmed virus infected group when compared with confirmed bacterial infected group. A combination of three lipids achieved an area under the receiver operating characteristic (ROC) curve of 0.911 (95% CI 0.81 to 0.98). This pilot study demonstrates the potential of metabolic biomarkers to assist clinicians in distinguishing bacterial from viral infection in febrile children, to facilitate effective clinical management and to the limit inappropriate use of antibiotics.

50. Plasma lipid profiles discriminate bacterial from viral infection in febrile children

Author

Wang, Xinzhu, Nijman, Ruud, Camuzeaux, Stephane, Sands, Caroline, Jackson, Heather, Kaforou, Myrsini, Emonts, Marieke, Herberg, Jethro A., Maconochie, Ian, Carrol, Enitan D., Paulus, Stephane C., Zenz, Werner, Van Der Flier, Michiel, De Groot, Ronald, Martinon-Torres, Federico, Schlapbach, Luregn J., Pollard, Andrew J., Fink, Colin, Kuijpers, Taco T., Anderson, Suzanne, Lewis, Matthew R., Levin, Michael, and McClure, Myra

Subjects
3. Good health
Abstract

Scientific reports 9(1), 17714 (2019). doi:10.1038/s41598-019-53721-1, Published by Macmillan Publishers Limited, part of Springer Nature, [London]

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