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22 results on '"Cantanhêde, Lilian Motta"'

3. Transcriptomics analysis highlights potential ways in human pathogenesis in Leishmania braziliensis infected with the viral endosymbiont LRV1

Author

Felipin, Kátia Paula, primary, Paloschi, Mauro Valentino, additional, Silva, Milena Daniela Souza, additional, Ikenohuchi, Yoda Janaina, additional, Santana, Hallison Mota, additional, Setúbal, Sulamita da Silva, additional, Rego, Cristina Matiele Alves, additional, Lopes, Jéssica Amaral, additional, Boeno, Charles Nunes, additional, Serrath, Suzanne Nery, additional, De Medeiros, Enmanuella Helga Ratier Terceiro, additional, Pimentel, Iasmin Ferreira, additional, Oliveira, Antonio Edson Rocha, additional, Cupolillo, Elisa, additional, Cantanhêde, Lilian Motta, additional, Ferreira, Ricardo de Godoi Matos, additional, and Zuliani, Juliana Pavan, additional

Published
2024
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6. First report of Leishmania (Mundinia) martiniquensis in South American territory and confirmation of Leishbunyavirus infecting this parasite in a mare

Author

Mendes Junior, Artur Augusto Velho, primary, Filgueira, Camila Patrício Braga, additional, Miranda, Luciana de Freitas Campos, additional, de Almeida, Adilson Benedito, additional, Cantanhêde, Lilian Motta, additional, Fagundes, Aline, additional, Pereira, Sandro Antônio, additional, Menezes, Rodrigo Caldas, additional, and Cupolillo, Elisa, additional

Published
2023
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8. First report of Leishmania (Viannia) lindenbergi causing tegumentary leishmaniasis in the Brazilian western Amazon region

Author

Cantanhêde Lilian Motta, Mattos Cristiane Batista, de Souza Ronconi Camila, Filgueira Camila Patrício Braga, da Silva Júnior Cipriano Ferreira, Limeira Claudino, de Jesus Silva Helen Paula, Ferreira Gabriel Eduardo Melim, Porrozzi Renato, Ferreira Ricardo de Godoi Mattos, and Cupolillo Elisa

Subjects
Leishmania (Viannia) lindenbergi, Tegumentary Leishmaniasis, Western Amazon, Infectious and parasitic diseases, RC109-216
Abstract

Tegumentary Leishmaniasis (TL) in the Brazilian Amazon region is associated with several Leishmania species. In this report, we describe two cases of TL related to Leishmania lindenbergi occurring in different locations of Rondônia state. After clinical diagnosis, lesion samples were collected for parasitological diagnoses via direct microscopic visualization, parasite isolation, and PCR. PCR reactions were positive in both clinical samples. Parasite isolation was possible for both patients, and isolates were submitted to species identification by isoenzyme electrophoresis and DNA sequencing. This report is the first to describe human infections caused by L. lindenbergi since the initial description and record of human infection by this species in 2002.

Published
2019
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9. Raising the suspicion of a non-autochthonous infection: identification of Leishmania guyanensis from Costa Rica exhibits a Leishmaniavirus related to Brazilian north-east and French Guiana viral genotypes

Author

Mata-Somarribas, Carlos, primary, Quesada-López, José, additional, Matamoros, María F, additional, Cervantes-Gómez, César, additional, Mejía, Annia, additional, Chacón, Karen, additional, Bendig, Ivannia, additional, Campos, Roger, additional, Quesada-Morera, Raphael, additional, Cantanhêde, Lilian Motta, additional, Pereira, Luiza de Oliveira R, additional, and Cupolillo, Elisa, additional

Published
2022
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10. Overcoming the Negligence in Laboratory Diagnosis of Mucosal Leishmaniasis

Author

Cantanhêde, Lilian Motta, primary, Mattos, Cristiane Batista, additional, Cruz, Ana Karoline, additional, Ikenohuchi, Yoda Janaina, additional, Fernandes, Flavia Gonçalves, additional, Medeiros, Enmanuella Helga Ratier Terceiro, additional, da Silva-Júnior, Cipriano Ferreira, additional, Cupolillo, Elisa, additional, Ferreira, Gabriel Eduardo Melim, additional, and Ferreira, Ricardo de Godoi Mattos, additional

Published
2021
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12. Comparison and clinical validation of qPCR assays targeting Leishmania 18S rDNA and HSP70 genes in patients with American Tegumentary Leishmaniasis

Author

Filgueira, Camila Patricio Braga, primary, Moreira, Otacilio Cruz, additional, Cantanhêde, Lilian Motta, additional, de Farias, Heloísa Martins Teixeira, additional, Porrozzi, Renato, additional, Britto, Constança, additional, Boité, Mariana Côrtes, additional, and Cupolillo, Elisa, additional

Published
2020
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13. First autochthonous case of canine visceral leishmaniasis in Rondônia, Brazil, a region with no history of visceral leishmaniasis

Author

Silva, Cristian José da, Mattos, Cristiane Batista, Felipin, Kátia Paula, Silva, Hélen Paula de Jesus, Cantanhêde, Lilian Motta, Porrozzi, Renato, Medeiros, Jansen Fernandes, and Ferreira, Ricardo de Godoi Mattos

Subjects
Canine visceral leishmaniasis, parasitic diseases, Autochthonous case, Leishmania infantum
Abstract

This report describes the first autochthonous case of canine visceral leishmaniasis in Rondônia, northern Brazil. A canine resident of the municipality of Cacoal, with clinical signs and symptoms of visceral leishmaniasis, was treated by a veterinarian. Samples were analyzed by a reference laboratory. Dual-path platform (DPP) assay, indirect immunofluorescence technique (IIT), enzyme-linked immunosorbent assay (ELISA), polymerase chain reaction (PCR), isolation in a culture medium, and direct parasitological analysis were performed. DPP assay, IIT, and ELISA revealed positive results for Leishmania; PCR identified the species as Leishmania infantum. Based on the clinical presentation and test results, canine visceral leishmaniasis was diagnosed.

Published
2018

17. MOLECULAR CHARACTERIZATION OF AMERICAN CUTANEOUS LEISHMANIASIS IN THE TRI‑BORDER AREA OF ASSIS BRASIL, ACRE STATE, BRAZIL

Author

TELES, Carolina Bioni Garcia, MEDEIROS, Jansen Fernandes, SANTOS, Ana Paula de Azevedo dos, FREITAS, Luís Antônio Rodrigues de, KATSURAGAWA, Tony Hiroshi, CANTANHÊDE, Lilian Motta, FERREIRA, Ricardo de Godoi Mattos, and CAMARG, Luís Marcelo Aranha

Subjects
Leishmania, PCR, Acre State
Abstract

SUMMARY In this study, Leishmaniaspecies were identified by Polymerase Chain Reaction (PCR). The epidemiology of patients suspected of having American Cutaneous Leishmaniasis in the municipality of Assis Brasil, Acre State, located in the Brazil/Peru/Bolivia triborder was also investigated. By PCR, the DNA of Leishmaniawas detected in 100% of the cases (37 samples) and a PCR-Restriction Fragment Length Polymorphism (RFLP) of the hsp 70gene identified the species in 32 samples: Leishmania (Viannia) braziliensis (65.6%) , L. (V.) shawi (28.1%) , L. (V.) guyanensis (3.1%) and mixed infection L. (V.) guyanensis and L. (Leishmania) amazonensis (3.1%)This is the first report of L. (V.) shawiand L. (L.) amazonensis in Acre. The two predominant species were found in patients living in urban and rural areas. Most cases were found in males living in rural areas for at least three years and involved in rural work. This suggests, in most cases, a possible transmission of the disease from a rural/forest source, although some patients had not engaged in activities associated with permanence in forestall areas, which indicate a possible sandflies adaptation to the periurban setting. RESUMO O presente estudo caracterizou as espécies de Leishmaniapela Reação em Cadeia da Polimerase (PCR). Também descreveu os aspectos epidemiológicos de pacientes com suspeita de leishmaniose tegumentar americana do município de Assis Brasil, Estado do Acre, Brasil, localizado na tríplice fronteira Brasil/Peru/Bolívia. A PCR detectou DNA de Leishmaniaem 100% dos casos (37 amostras) e a PCR- Restriction Fragment Length Polymorfism(RFLP) do gene hsp 70identificou as espécies em 32 amostras: Leishmania (Viannia) braziliensis (65,6%) , L. (V.) shawi (28,1%) , L. (V.) guyanensis (3,1%) e infecção mista L. (V.) guyanensis e L. (Leishmania) amazonensis(3,1%)Esse é o primeiro registro de L. (V.) shawie L. (L.) amazonensis no Acre. As duas espécies predominantes foram encontradas em indivíduos residentes em áreas rurais e urbanas. O maior número de casos foi notificado entre indivíduos de áreas rurais, sexo masculino, de ocupação rural e tempo de residência maior que três anos. Esses dados sugerem possível transmissão da doença em ambiente rural/florestal na maioria dos casos, no entanto alguns pacientes não tinham envolvimento com atividades relacionadas com a permanência na floresta, indicando possível adaptação de flebotomíneos no ambiente periurbano.

Published
2015

18. Further Evidence of an Association between the Presence of Leishmania RNA Virus 1 and the Mucosal Manifestations in Tegumentary Leishmaniasis Patients

Author

Cantanhêde, Lilian Motta, primary, da Silva Júnior, Cipriano Ferreira, additional, Ito, Marcos Massayuki, additional, Felipin, Kátia Paula, additional, Nicolete, Roberto, additional, Salcedo, Juan Miguel Villalobos, additional, Porrozzi, Renato, additional, Cupolillo, Elisa, additional, and Ferreira, Ricardo de Godoi Mattos, additional

Published
2015
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19. MOLECULAR CHARACTERIZATION OF AMERICAN CUTANEOUS LEISHMANIASIS IN THE TRI-BORDER AREA OF ASSIS BRASIL, ACRE STATE, BRAZIL.

Author

Garcia TELES, Carolina Bioni, MEDEIROS, Jansen Fernandes, dos SANTOS, Ana Paula de Azevedo, de FREITAS, Luís Antônio Rodrigues, KATSURAGAWA, Tony Hiroshi, CANTANHÊDE, Lilian Motta, Mattos FERREIRA, Ricardo de Godoi, and Aranha CAMARGO, Luís Marcelo

Subjects
CUTANEOUS leishmaniasis, POLYMERASE chain reaction, EPIDEMIOLOGICAL research, PUBLIC health, RESTRICTION fragment length polymorphisms, LEISHMANIA
Abstract

Copyright of Revista do Instituto de Medicina Tropical de São Paulo is the property of Instituto de Medicina Tropical de Sao Paulo and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Published
2015
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21. Raising the suspicion of a non-autochthonous infection: identification of Leishmania guyanensis from Costa Rica exhibits a Leishmaniavirus related to Brazilian north-east and French Guiana viral genotypes.

Author

Mata-Somarribas C, Quesada-López J, Matamoros MF, Cervantes-Gómez C, Mejía A, Chacón K, Bendig I, Campos R, Quesada-Morera R, Cantanhêde LM, Pereira LOR, and Cupolillo E

Subjects
Humans, Brazil epidemiology, Costa Rica, French Guiana, Genotype, Leishmania guyanensis genetics, Leishmaniasis, Cutaneous parasitology, Leishmaniavirus genetics
Abstract

Background: Costa Rica has a history of neglecting prevention, control and research of leishmaniasis, including limited understanding on Leishmania species causing human disease across the country and a complete lack of knowledge on the Leishmania RNA virus, described as a factor linked to the worsening and metastasis of leishmanial lesions., Objectives: The aim of this work was to describe a case of cutaneous leishmaniasis by Leishmania (Viannia) guyanensis, bearing infection with Leishmaniavirus 1 (LRV1) in Costa Rica, raising the suspicion of imported parasites in the region., Methods: The Leishmania strain was previously identified by routine hsp70 polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) in Costa Rica and subsequently characterised by isoenzyme electrophoresis and Sanger sequencing in Brazil. Screening for LRV1 was conducted with a dual RT-PCR approach and sequencing of the fragment obtained., Findings: Since 2016 Costa Rica performs Leishmania isolation and typing as part of its epidemiological surveillance activities. Amongst 113 strains typed until 2019, only one was characterised as a L. (V.) guyanensis, corresponding to the first confirmed report of this species in the country. Interestingly, the same strain tested positive for LRV1. Sequencing of the viral orf1 and 2, clustered this sample with other LRV1 genotypes of South American origin, from the Northeast of Brazil and French Guiana., Main Conclusion: The unique characteristics of this finding raised the suspicion that it was not an autochthonous strain. Notwithstanding its presumed origin, this report points to the occurrence of said endosymbiont in Central American Leishmania strains. The possibility of its local dispersion represents one more challenge faced by regional health authorities in preventing and controlling leishmaniasis.

Published
2023
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22. MOLECULAR CHARACTERIZATION OF AMERICAN CUTANEOUS LEISHMANIASIS IN THE TRI‑BORDER AREA OF ASSIS BRASIL, ACRE STATE, BRAZIL.

Author

Teles CB, Medeiros JF, Santos AP, Freitas LA, Katsuragawa TH, Cantanhêde LM, Ferreira Rde G, and Camarg LM

Subjects
Adolescent, Adult, Animals, Brazil epidemiology, Child, Child, Preschool, Female, Humans, Infant, Infant, Newborn, Leishmania genetics, Leishmania isolation & purification, Leishmaniasis, Cutaneous diagnosis, Leishmaniasis, Cutaneous epidemiology, Leishmaniasis, Mucocutaneous diagnosis, Leishmaniasis, Mucocutaneous epidemiology, Male, Polymerase Chain Reaction, Polymorphism, Restriction Fragment Length, Risk Factors, Rural Population, Urban Population, Young Adult, Leishmania classification, Leishmaniasis, Cutaneous parasitology, Leishmaniasis, Mucocutaneous parasitology
Abstract

In this study, Leishmania species were identified by Polymerase Chain Reaction (PCR). The epidemiology of patients suspected of having American Cutaneous Leishmaniasis in the municipality of Assis Brasil, Acre State, located in the Brazil/Peru/Bolivia tri-border was also investigated. By PCR, the DNA of Leishmania was detected in 100% of the cases (37 samples) and a PCR-Restriction Fragment Length Polymorphism (RFLP) of the hsp 70 gene identified the species in 32 samples: Leishmania (Viannia) braziliensis (65.6%) , L. (V.) shawi (28.1%) , L. (V.) guyanensis (3.1%) and mixed infection L. (V.) guyanensis and L. (Leishmania) amazonensis(3.1%)This is the first report of L. (V.) shawi and L. (L.) amazonensis in Acre. The two predominant species were found in patients living in urban and rural areas. Most cases were found in males living in rural areas for at least three years and involved in rural work. This suggests, in most cases, a possible transmission of the disease from a rural/forest source, although some patients had not engaged in activities associated with permanence in forestall areas, which indicate a possible sandflies adaptation to the periurban setting.

Published
2015
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