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Paolo Talamucci, Full Professor of Range Management at the Università degli Studi in Florence from 1975 to 2001, passed away on 27th November 2009, after a brief and unrelenting illness. Professor Talamucci spent many long years in the field of research, in both Mediterranean and Alpine regions. His research, profoundly rooted in the territory he was studying, holistic in its approach and rigorously scientific, was characterized by a strong interdisciplinary element, seeking always to take into account the totality of the components of the ecosystem. Professor Talamucci was one of the first scholars of the pastoral system to highlight the problems related to the abandonment of traditional animal husbandry in mountainous zones and to look for ways to counteract the undesirable transformation of the landscape and the consequent loss of biodiversity. His curriculum and his numerous scientific publications bear testimony not only to the prestigious research institutes, world-wide, with which he was associated, but also to the important international dimension of his research.

This paper presents the results of a forest cover analysis over a time span of 150 years in a protected area of Eastern Trentino (Northern Italy), Paneveggio Pale di S. Martino Nature Park. With the aid of GRASS GIS two historical maps (1859 and 1936) and a set of aerial photographs taken from 1945 to 2006 have been analysed, orthorectified and classified with a supervised method, in order to derive a series of forest cover maps. Techniques applied and problems encountered in using heterogeneous material are discussed. The research shows that from 1859 to the present the increase of forest cover is about 25%, due to the reduced impact of forestry and farming. Timberline dynamics have also been considered; an average growth of about 1 m/year has been estimated for the last 150 years and the data have been compared with the timberline cartography and to field surveys. Timberline estimation for recent years appears to be affected mainly by lower human pressure while the relationship with climate changes is difficult to evaluate. Landscape metrics were used to quantify the changes in forest fragmentation and to identify three core areas that have remained unchanged over time. This case study fills a gap of knowledge about the history of forest cover in the area, shows how multi temporal analysis can support protected area management. This study has been requested by the Park managers, a sign t that landscape planners are becoming aware of past landscape importance. [ABSTRACT FROM AUTHOR]

The topical treatment for oral mucosal diseases is often based on products optimized for dermatologic applications; consequently, a lower therapeutic effect may be present. 18-β-glycyrrhetic acid (GA) is extracted from Glycirrhiza glabra. The first aim of this study was to test the cytotoxicity of GA on PE/CA-PJ15 cells. The second aim was to propose and test two different delivery systems, i.e. nanoparticles and fibers, to guarantee a controlled release of GA in vitro. We used chitosan and poly(lactic-co-glycolic) acid based nanoparticles and polylactic acid fibers. We tested both delivery systems in vitro on PE/CA-PJ15 cells and on normal human gingival fibroblasts (HGFs). The morphology of GA-loaded nanoparticles (GA-NPs) and fibers (GA-FBs) was investigated by electron microscopy and dynamic light scattering; GA release kinetics was studied spectrophotometrically. MTT test was used to assess GA cytotoxicity on both cancer and normal cells. Cells were exposed to different concentrations of GA (20-500 μmol l -1 ) administered as free GA (GA-f), and to GA-NPs or GA-FBs. ROS production was evaluated using dichlorodihydrofluorescein as a fluorescent probe. Regarding the cytotoxic effect of GA on PE/CA-PJ15 cells, the lowest TC 50 value was 200 μmol l -1 when GA was added as GA-NPs. No cytotoxic effects were observed when GA was administered to HGFs. N-acetyl Cysteine reduced mortality induced by GA-f in PE/CA-PJ15 cells. The specific effect of GA on PE/CA-PJ15 cells is mainly due to the different sensitivity of cancer cells to ROS over-production; GA-NPs and GA-FBs formulations increase, in vitro, this toxic effect on oral cancer cells.

Background and Objective: Metabolic syndrome (MetS) is associated with an increased risk of periodontitis even if the mechanism is unknown. Since both MetS and periodontitis are characterized by an alteration of inflammation status, the aim of this pilot study was to determine if differences in ROS metabolism of phagocytes isolated from (A) patients with MetS, (B) patients with both MetS and mild periodontitis, (C) healthy subjects and (D) normal weight subjects with mild periodontitis, were present., Methods: ROS metabolism was studied by a Chemiluminescence (CL) technique: the system was made up of luminol (100 nmol/L) and cells (1 × 10 5 ) in the presence or absence of stimulus constituted by opsonized zymosan (0.5 mg). The final volume (1.0 mL) was obtained using modified KRP buffer. ROS production was measured at 25°C for 2 h, using an LB 953 luminometer (Berthold, EG & G Co, Germany). All the experiments were performed in triplicate., Statistical Analysis: All results are mean ± standard deviation (SD). The group of means was compared by the analysis of variance "(ANOVA)". A value of p < 0.05 was considered significant., Results: Results showed that basal ROS production (both from PMNs and from PBMs) of groups A, B and D was increased with respect to that obtained from group C ( p <0.05)., Conclusion: These results are congruent with literature data, although the actual clinical relevance of the phenomenon remains to be evaluated.