Search

The glutamine synthetase/glutamic acid synthetase (GS/GOGAT) cycle plays important roles in N metabolism, growth, development, and stress resistance in plants. Excess ammonium (NH4+) restricts growth, but GS can help to alleviate its toxicity. In this study, the 84K model clone of hybrid poplar (Populus alba × P. tremula var. glandulosa), which has reduced biomass accumulation and leaf chlorosis under high-NH4+ stress, showed less severe symptoms in transgenic lines overexpressing GLUTAMINE SYNTHETASE 1;2 (GS1;2 -OE), and more severe symptoms in RNAi lines (GS1;2 -RNAi). Compared with the wild type, the GS1;2 -OE lines had increased GS and GOGAT activities and higher contents of free amino acids, soluble proteins, total N, and chlorophyll under high-NH4+ stress, whilst the antioxidant and NH4+ assimilation capacities of the GS1;2 -RNAi lines were decreased. The total C content and C/N ratio in roots and leaves of the overexpression lines were higher under stress, and there were increased contents of various amino acids and sugar alcohols, and reduced contents of carbohydrates in the roots. Under high-NH4+ stress, genes related to amino acid biosynthesis, sucrose and starch degradation, galactose metabolism, and the antioxidant system were significantly up-regulated in the roots of the overexpression lines. Thus, overexpression of GS1;2 affected the carbon and amino acid metabolism pathways under high-NH4+ stress to help maintain the balance between C and N metabolism and alleviate the symptoms of toxicity. Modification of the GS/GOGAT cycle by genetic engineering is therefore a potential strategy for improving the NH4+ tolerance of cultivated trees. [ABSTRACT FROM AUTHOR]

Proton-exchange-membrane fuel cells (PEMFCs) are attractive next-generation power sources for use in vehicles and other applications.sup.1, with development efforts focusing on improving the catalyst system of the fuel cell. One problem is catalyst poisoning by impurity gases such as carbon monoxide (CO), which typically comprises about one per cent of hydrogen fuel.sup.2-4. A possible solution is on-board hydrogen purification, which involves preferential oxidation of CO in hydrogen (PROX).sup.3-7. However, this approach is challenging.sup.8-15 because the catalyst needs to be active and selective towards CO oxidation over a broad range of low temperatures so that CO is efficiently removed (to below 50 parts per million) during continuous PEMFC operation (at about 353 kelvin) and, in the case of automotive fuel cells, during frequent cold-start periods. Here we show that atomically dispersed iron hydroxide, selectively deposited on silica-supported platinum (Pt) nanoparticles, enables complete and 100 per cent selective CO removal through the PROX reaction over the broad temperature range of 198 to 380 kelvin. We find that the mass-specific activity of this system is about 30 times higher than that of more conventional catalysts consisting of Pt on iron oxide supports. In situ X-ray absorption fine-structure measurements reveal that most of the iron hydroxide exists as Fe.sub.1(OH).sub.x clusters anchored on the Pt nanoparticles, with density functional theory calculations indicating that Fe.sub.1(OH).sub.x-Pt single interfacial sites can readily react with CO and facilitate oxygen activation. These findings suggest that in addition to strategies that target oxide-supported precious-metal nanoparticles or isolated metal atoms, the deposition of isolated transition-metal complexes offers new ways of designing highly active metal catalysts.Atomically dispersed iron hydroxide deposited on silica-supported platinum nanoparticles enables complete and selective carbon monoxide removal through preferential oxidation in hydrogen in the temperature range from 198 to 380 kelvin., Author(s): Lina Cao [sup.1] [sup.3] , Wei Liu [sup.2] , Qiquan Luo [sup.1] [sup.3] , Ruoting Yin [sup.1] [sup.3] , Bing Wang [sup.1] [sup.3] , Jonas Weissenrieder [sup.4] , Markus [...]

Natural gas is an eco-friendly energy source with low carbon emissions, making it attractive globally. Understanding gas reservoirs is crucial for sustainable extraction and optimizing potential. However, the complicated fluid flow and production dynamics within intricate gas reservoirs, particularly those characterized by abnormally high pressures and tight porous media, remain partially understood and demand further investigation. In a tight porous medium subjected to high pressure, the assumption of constant permeability is no longer valid. Consequently, a novel composite seepage model has been developed in this study, which considers the responsiveness of permeability to stress. Perturbation theory is employed to address the inherent non-linearity demonstrated by the permeability modulus. The solution of dimensionless pressure responses under constant production conditions is accomplished in the Laplace domain by implementing integral transformation methods. Overall, a comprehensive model is provided to understand the production behaviors of tight gas reservoirs. Moreover, in order to comprehend the transient flow characteristics of tight gas reservoirs, log–log plots are generated through the Stehfest numerical inversion approach, with the flow regimes categorized based on the normalized time phases of the pressure curves. Parametric investigations reveal that stress sensitivity detrimentally affects permeability, resulting in more pronounced pressure declines during the intermediate and late flow phases. The transient seepage model elaborated in this study is able to consider the pertinent formation and well parameters. These interpreted parameters bear significance in designing hydraulic fracturing operations, assessing the potential of tight gas reservoirs, and ultimately enhancing gas production. The presented model not only enhances our understanding of the behavior of horizontal wells in stress-sensitive tight gas reservoirs but also makes a valuable contribution to the broader discussion on transient flow phenomena in petroleum engineering. [ABSTRACT FROM AUTHOR]