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10 results on '"Capella, Virginia"'

1. Hydrogels for Biomedical Applications

Author

Liaudat, A. C., primary, Capella, Virginia, additional, Broglia, Martin F., additional, Molina, Maria A., additional, Barbero, Cesar A., additional, Bosch, Pablo, additional, Rodríguez, Nancy, additional, and Rivarola, Claudia R., additional

Published
2024
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7. Interaction Between Hyaluronic Acid Semi‐Interpenetrated Hydrogel with Bull Spermatozoa: Studies of Sperm Attachment–Release and Sperm Quality

Author

Blois, Damian A., primary, Liaudat, Ana C., additional, Capella, Virginia, additional, Morilla, Gricelda, additional, Rivero, Rebeca E., additional, Broglia, Martin F., additional, Barbero, Cesar A., additional, Rodríguez, Nancy, additional, Bosch, Pablo, additional, and Rivarola, Claudia R., additional

Published
2021
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9. Bull sperm selection by attachment to hyaluronic acid semi‐interpenetrated hydrogels.

Author

Liaudat, Ana Cecilia, Blois, Damian, Capella, Virginia, Morilla, Gricelda, Rivero, Rebeca, Barbero, César, Rodríguez, Nancy, Rivarola, Claudia, and Bosch, Pablo

Subjects
REPRODUCTIVE technology, HYDROGELS, SPERMATOZOA, HYALURONIC acid
Abstract

We report the development of a hydrogel‐based approach to select bull spermatozoa, a crucial step for successful assisted reproductive techniques (ARTs). Hyaluronic acid (HA) semi‐interpenetrated N‐isopropylacrylamide (PNIPAM) co‐20% N‐Tris (hydroxymethyl) methyl acrylamide (HMA) hydrogels were synthetized on glass surfaces and cultured in presence of frozen‐thawed bull spermatozoa. A fraction of motile bull spermatozoa population strongly attached to hydrogels and was partially released by treatment with hyaluronidase. Fifty‐nine (59 ± 7.24) per cent of sperm cells attached to PNIPAM‐HMA‐HA hydrogels and 31.16 ± 4.81% of them were released upon treatment with medium containing hyaluronidase. This attached‐released sperm fraction has acceptable characteristics of progressive motility (50.0 ± 5.0%), vigour (4), high viability (58.7 ± 11.7%) and low percentage of acrosome reacted spermatozoa (23.36 ± 4.1%). Our findings indicate that PNIPAM‐HMA‐HA hydrogels are non‐toxic and allow the selection of high‐quality sperm cells for ART. [ABSTRACT FROM AUTHOR]

Published
2022
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