1. Detection of IncN-pST15 one-health plasmid harbouring bla KPC-2 in a hypermucoviscous Klebsiella pneumoniae CG258 isolated from an infected dog, Brazil.
- Author
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Sellera FP, Fuga B, Fontana H, Esposito F, Cardoso B, Konno S, Berl C, Cappellanes MH, Cortez M, Ikeda M, de Souza CM, Cerdeira L, and Lincopan N
- Subjects
- Animals, Anti-Bacterial Agents pharmacology, Bacterial Proteins genetics, Brazil epidemiology, Dogs, Klebsiella pneumoniae genetics, Microbial Sensitivity Tests veterinary, Plasmids genetics, beta-Lactamases genetics, Dog Diseases epidemiology, Klebsiella Infections epidemiology, Klebsiella Infections veterinary, One Health
- Abstract
The emergence and rapid spread of carbapenemase-producing Enterobacterales represents a serious public health concern. Critically, these global priority bacteria have begun to be reported in companion animals, implying a potential risk of cross-transmission between humans and pets. Using long-read (MinION) and short-read (Illumina) sequencing technologies, we have identified and characterized a hypermucoviscous KPC-2-producing Klebsiella pneumoniae strain belonging to the high-risk international clone ST11/CG258, in a dog with urinary tract infection. Strikingly, the bla
KPC-2 gene was carried by a 54-kb IncN plasmid assignated to ST15, which shared 99.8 and 96.8% pairwise identity with IncN-pST15 plasmids from human and environmental K. pneumoniae strains, respectively; all come from an area with high endemicity of KPC-2. Our findings suggest that IncN-pST15 plasmids conferring carbapenem resistance can play as important a role as clonal transmission of K. pneumoniae, representing another major challenge for One Health., (© 2021 The Authors. Transboundary and Emerging Diseases published by Wiley-VCH GmbH.)- Published
- 2021
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