Search

The aim of this work was to investigate prolonged storage at 4˚ C on the survival of cat cumulus oocyte complexes. The ovaries were obtained from 20 domestic cats. The ovaries were evaluated at different time intervals. The viability test for cumulus oocyte complexes was performed at 2 hours, 24 hours and 72 hours after being sampled. Tests for the viability of cumulus oocyte complexes were performed with Neutral red and Trypan blue. Storage conditions for cumulus oocyte complexes are a critical step in establishing fertility conservation protocols in animals, as well as for assisted reproduction. [ABSTRACT FROM AUTHOR]

The integration of Romania into the European Union requires special attention to the hunting populations. The observed size of an animal population, as opposed to the genetic size, is given by the number of individuals in all categories, as well as by the total number of males and females participating in the production of the descendant generation. The purpose of the present paper was to study the evolution of the observed sizes of the hunting population for 13 species of animals in the period 2016-2020, on hunting area 16-Sarlota, from Timis County, with a total area of 6.149 ha. The present study highlighted that on this hunting area, Cervidae family - which includes ruminants with deciduous horns, is represented by 3 species, namely Red Deer (Cervus elaphus L), Fallow Deer (Dama dama L), and Roe Deer (Capreolus carpeolus L). Our paper concludes that the decrease of the populations of Red Deer (Cervus elaphus L) species, Fallow Deer (Dama dama L) species, Roe Deer (Capreolus capreolus L) species, Wild hog (Sus scrofa L.) species, The European hare (Lepus europaeus P) species, and Common Pheasant (Phasianus colchicus L.) species, in the studied period of 2018-2019, is due to the increase of the observed size with 200% on this hunting area of Jackal (CaniȘ aureus L.) species, and also due to the significant presence of the Red Fox (Vulpes vulpes L) species. We recommend the permanent monitoring and limitation of populations from the Canidae family, especially of the Jackal (CaniȘ aureus L.) species. For the other identified species, the population of evolutionary numbers showed that there is a good correlation between the number of individuals and their biogenic capacity. The present study recommends the revival of the existing population on this hunting area, through "blood refreshing" actions. [ABSTRACT FROM AUTHOR]

Romanian Mioritic Shepherd Dog was selected from a natural population breed in Carpathian Mountains of Romania. The aim of this study was to analyze the existence and size of sexual dimorphism in a population of 26 males and 23 females of the Mioritic Shepherd Dog breed, for 10 body measurements: withers height, middle back height, croup height (iliac), base of tail height (coccyx), croup width, body length, tail length, thorax depth, thorax width and thorax perimeter. The animals were registered with the Romanian Mioritic Association Club from Romania. Following the study on the significance of statistical differences between body measurements recorded in 26 males and 23 females, it was concluded that sexual dimorphism is evident in the population of the Romanian Mioritic shepherd dog studied in this paper. We recommend that dog breeders of this breed take into account the genetic improvement programs, also the results presented in this paper. [ABSTRACT FROM AUTHOR]

Honey is a bee product that has antimicrobial activity, between different types of honey there being differences in terms of effectiveness and mechanisms of action against the same type of microorganism. Given the constant increase in the number of antibiotic-resistant bacteria and the need to find natural alternatives to them, the purpose of this study was to evaluate the antibacterial activity of polyfloral honey in western Romania. Honey was tested in the following concentrations: 80%; 60%; 40%; 20%; 10%; 5%. Two Gram+ bacterial strains (Staphylococcus aureus ATCC 29213, Streptococcus pneumoniae ATCC 49619) and three Gram- strains (Enterococccus faecalis ATCC 29213, Escherichia coli ATCC 25922 and Salmonella typhimurium ATCC 14028) were used in the study. The results of this study show that polyfloral honey from the western part of Romania has an antiproliferative effect on the bacterial strains tested, depending on the applied concentration. The highest inhibition rates were recorded on Salmonella typhimurium, Streptococcus pneumoniae and Staphylococcus aureus, and the lowest values were recorded in the bacterial strains Enterococcus faecalis and Escherichia coli. [ABSTRACT FROM AUTHOR]

In biotechnologies, genetics is an important issue in preserving the animal and plant species, and also in medical diagnostic. Epigenetic is one branch of genetics that studies the variation of phenotypic traits which can be the consequences of different factors, especially the environmental factors. And, because of the epigenetic is dealing with the gens, which are biochemically defined by proteins' metabolism, we can say that there is a special interrelation between epigenetic and metabolism. Changes in the metabolism of some biochemical compounds can be transferred to gene expression -- which can be also altered. The latest experimental studies published in different scientific journals proved that epigenetic is closely related to the phenotypic traits and to the environment. [ABSTRACT FROM AUTHOR]

The observed size of an animal population, as opposed to the genetic size, is given by the number of individuals in all categories as well as by the total number of males and females participating in the production of the descendant generation. The integration of Romania into the European Union requires special attention to the hunting populations. The purpose of the present paper was to study the evolution of the observed sizes of the hunting population for 13 species of animals in the period 2015-2019, on 17 hunting areas Nades, from Timis County with a total area of 9.325 ha. The present study highlighted that on this hunting area, Cervidae family -- which includes ruminants with deciduous horns, is represented by 3 species, namely Red Deer (Cervus elaphus L), Fallow Deer (Dama dama L), and Roe Deer (Capreolus capreolus L). Also, it is concluded that it is permanently effective of European swan (Lepus europaeus P.), it is more important to choose the significant growth on this hunting area with 100% a Jackal (Canis aureus L.), at the beginning of 2018, compared to the year 2017. For the other identified species, the population of evolutionary numbers showed that there is a good correlation between the number of individuals and their biogenic capacity. The present study recommends the revival of the existing population on this hunting ground, through "blood refreshing" actions. [ABSTRACT FROM AUTHOR]

Chestnuts (Castanea sativa) are traditionally ingredients of different food and feed, being used in human and animal diet - due to its valuable active principles. The products used are chestnuts (fruits) and also by-products from chestnuts industry like shell, skin, bark, leaves or even flowers. Chestnuts are ingredients with protein - but glutenfree, with low lipid content, high carbohydrates, with hydro-soluble vitamins (A, B1, B2, B3, B6, B12, C) and minerals (Ca, Fe, Mg, P, K, Na, Zn). Also, in chestnut products are present different antioxidants which could be extracted and used in processing of food and feed products. Thus, various extraction techniques and solvents are used to obtain good yield of substances that are used in animal feeding (for broiler chicken, pigs, rabbits). [ABSTRACT FROM AUTHOR]

Integration of the Romania in the European Union is imposing a special attention to hunting populations. The aim of the present paper was to study the quantitative evolution of the hunting population for 13 animal species during 2014-2018, on 20 Bara hunting area, from Timis County, heaving a total area of 10.889 ha. The study shows that form the 13 monitored animal species, 3 were not identified on this hunting area: Fallow Deer (Dama dama L), Wildcat (Felis silvestris L), and Muskrat (Ondatra zibethica L.). The study has highlighted the emergence of an immigrant species, Jackal (Caniş aureus L.), with a constant population of 5 individuals at the beginning of the study period - 2014, which caused a decrease in the number of the Red Fox (Vulpes vulpes L) herds, with a relative value of 57.7%, the two species being competing. For the other species identified, the population of evolution number demonstrated that there is a good correlation between the number of individuals and its biogenic capacity. [ABSTRACT FROM AUTHOR]

Recent developments in understanding the physiological mechanisms of reproduction control can be adapted to swine reproductive management. This article points out the procedures that can be used to induce estrus, in prepubescent and anestrus gilts, and heat synchronization. For estrus induction and heat synchronization, we used P.G. 600 commercial product (400 IU of PMSG and 200 IU of hCG, Intervet, Millsboro, DE). A total of 36 gilts, from Large White breed, were used, divided in two lots: pre-pubescent gilts (5months of age, no=19) and gilts in anestrus (8 months of age, no=17). The efficacy of hormonal P.G. 600 product was different for the two lots. Estrus manifestation rate was different between lots 73.68% of pre-pubescent gilts compared with 76.47% of anestrus gilts. The use of P.G. 600 hormonal product is effective in inducing and synchronizing estrus in gilts. [ABSTRACT FROM AUTHOR]

Bioactive compounds from natural sources can act as oxygen free radical scavengers or metal chelators, which enables them to be used as natural antagonists to heavy metals toxicity. So the present study was carried out to compare histologically the aspect of intestine tissue of Prussian carp specimens, subjected to chronic Cd intoxication with and without garlic, cilantro and chlorella dietary supplementation.150 Prussian carps, with weight of 10-12 g were divided according to the following treatments for 21 days: C (without treatment), E1 (10 ppm Cd into water), E2 (10 ppm Cd into water+2% lyophilized garlic in feed), E3 (10 ppm Cd into water+2% lyophilized cilantro in feed), E4 (10 ppm Cd into water+2% lyophilized chlorella in feed). Cadmium toxicity and the potential protective effect of the three lyophilized products against the impact of cadmium toxicity were histopathologically assessed. For this purpose, fragments of intestine were removed and routinely processed at the end of experimental period and analyzed in light microscopy. A specific QuickPHOTO Micro 2.2 software has been used for the histological study. Tissue alterations were assessed using the histopathological score ranging from - to +++ depending on the degree and extend of lesions: (-) none, (+) mild occurrence, (++) moderate occurrence, (+++) severe occurrence. Our research findings show that Cd induces a significant increase in histopathological changes like vascular network hypertrophies and reach infiltrating leukocyte cells. In the same time, chlorella powder added to the fish diet, expressed the most effectiveness on the intestinal recovery of the cadmium-intoxicated fish followed by while cilantro and garlic powder. [ABSTRACT FROM AUTHOR]

The purpose of our work was to compare the efficiency of two programs for the induction and synchronization of estrous and ovulation in acyclic cows (Ovsynch and Select Synch). To the first batch of 18 cows we have given 2ml Cystorelin (100 mcg GnRH) on day 0, on day 7th 2ml Estrumate (500 mcg of Cloprostenol, on the ninth day we administered 2ml Cystorelin (100 mcg GnRH), after 16 hours we conducted artificial insemination. To the second group of 14 cows we have given 2ml Cystorelin (100 mcg GnRH) on day 0, on day 7th we administered 2ml Estrumate (500 mcg of cloprostenol), heat detection was made at 24-48 hours after PGF2α and continued 5 to 7 days. Cows were inseminated at 8-12 hours after estrous detection. 60 days after the artificial insemination, 11 cows (44%) of the first group and 9 cows (41%) of the second batch were diagnosed as pregnant. These two protocols of inducing and synchronizing estrous are very effective in acyclic cows. [ABSTRACT FROM AUTHOR]

Recent developments in understanding the physiological mechanisms of reproduction control can be adapted to swine reproductive management. This article points out the procedures that can be used to induce estrus, in prepubescent and anestrus gilts, and heat synchronization. For estrus induction and heat synchronization, we used P.G. 600 commercial product (400 IU of PMSG and 200 IU of hCG, Intervet, Millsboro, DE). A total of 36 gilts, from Large White breed, were used, divided in two lots: pre-pubescent gilts (5months of age, no = 19) and gilts in anestrus (8 months of age, no = 17). The efficacy of hormonal P.G. 600 product was different for the two lots. Estrus manifestation rate was different between lots 73.68% of pre-pubescent gilts compared with 76.47% of anestrus gilts. The use of P.G. 600 hormonal product is effective in inducing and synchronizing estrus in gilts. [ABSTRACT FROM AUTHOR]

Administration of GnRH in the luteal phase of estrous in dairy cows induces an increase of LH levels, with the modification of the growth waves of the ovarian follicles. GnRH induces ovulation or atresia of the dominant follicle and the recruitment of a new wave of follicular growth. The GnRH administration in the luteal phase of the estrous cycle induces growth waves synchronization of ovarian follicles, so that, a new wave of follicles started to grow at 5-6 days after administration. In our experiments, we administered 2ml Ovarelin (100 mcg GnRH), to three groups of cows (116 cows): in early luteal phase of the estrous cycle (days 4-5), in the middle of luteal phase (days 9-12), and late luteal phase (days 15-16). The rates of cows standing heat were 91.66% when GnRH was administered in early luteal phase, 95.22% when was administrated in the middle of luteal phase, and 73.68% in late luteal phase GnRH administration. After artificial insemination, the conception rates were 48.48% in early luteal phase, 52.5% at middle luteal phase and 46.42% in the late luteal phase of the estrous cycle. [ABSTRACT FROM AUTHOR]

In this study we assessed the consumption speed (g/min), as well as the required time for consumption of 1 kg of raw forage for alfalfa-hay, herbs silage, beet and concentrates), administered in three portions (P1, P2, and P3) per day, in 2 equal feedings (F1, F2) per portion., to Romanian Black and White cows. The administration order of forages (fibrous-succulents and succulents-fibrous), the number of feedings and the average consumption speed among the three portions were the experimental variants. Data was computed by ANOVA/MANOVA. The highest consumption speed was registered for herbs silage (237.41 g/min), followed by the beets (233.43 g/min), concentrates (180.89) and alfalfa hay (48.89 g/min). The required time for 1 kg of raw forage consumption (minutes and seconds) was 5.32 for concentrates, 20.27 for alfalfa hay, 4.13 for herbs silage and 4.17 for beets. [ABSTRACT FROM AUTHOR]

Puerperal period is the most critical period from the productive life of females, at the borderline between physiologic and pathologic. In this stage some reproductive turbulence may appear (uterine subinvolution, uterine infections, ovary inactivity, etc.), which may lead to the increase of the interval between calving and first estrus manifestation. The aim of our paper was to reduce the interval between calving and the firs estrus, also the reduction of the incidence of some impediments of the reproductive function in cows, after calving. For this purpose 500 mcg Cloprostenol were administered at different intervals from calving (12, 24, hours and 7 days). At experimental lots, the interval from calving to first estrus was, on average, 37.57 days, and at control lot it was 45.2 days. Regarding to the impairing of the reproductive function, uterine subinvolution, evaluated at 14 days from calving, it was 26.78% at experimental lots and 33.33% at control lot, and uterine infections were, on average, 14.8% at experimental lots and 16.2% at control lot. In conclusion, administration of synthetic analogues of PgF2α, in the first week after calving, has a negative influence on puerperal period and interval from calving to first estrus manifestation. [ABSTRACT FROM AUTHOR]

As a research on the reproductive physiology and endocrinology surrounding the estrous cycle in dairy cattle has been compiled, several estrous synchronization programs have been developed for use with dairy cows. These include several programs that facilitate the mass breeding of all animals at a predetermined time (timed-AI) rather than the detection of estrus. We studied on 15 dary cows which were synchronized by Ovsynch and Cosynch programs. The estrus response for cows in Ovsynch protocol was of 63%. Pregnancy per insemination at 60 days was of 25%. Estrus response for cow in Cosynch protocol was of 57%. Pregnancy per insemination at 60 days was of 57%. Synchronization of ovulation using Ovsynch protocols can provide an effective way to manage reproduction in lactating dairy cows by eliminating the need for estrus detection. These are really efficient management programs for TAI of dairy cows that are able to reduce both the labour costs and the extra handling to daily estrus detection and AI. [ABSTRACT FROM AUTHOR]

At dairy cows, the increase in milk production is associated with the decrease of heat manifestation and conception rates. GnRH is mostly used for treatment of different problems of the reproductive function and for improving the pregnancy rates in cows. The aim of our paper was to contribute to increase of conception rates, at cows with ovarian activity, at first AI after calving. The experiments were conducted on 58 cows, from Romanian Black Spotted breed (Frezian) and 53 cows from Romanian Spotted breed (Simmental). The animals were divided into lots as follows: for Romanian Black Spotted breed 33 of the cows in were in experimental lot and 25 were in control lot, for Romanian Spotted breed 29 ere in experimental lot and 24 were in control lot. The females form experimental lots were treated with 100 mcg (2ml) Ovarelin (GnRH), at the first AI, after VWP. At the cows form Romanian Black Spotted, from the 33 females in experimental lot, 12 did not return into heat after insemination, which represents a conception rate of 36.4%. At the cows form Romanian Spotted, form the 29 cows in experimental lot 8 did not return into heat after insemination, representing a conception rate of 44.8%. Administration of 100 mcg GnRH (2 ml Ovarelin) at the time of AI determines a significant increase of the conception rate with 8.4-11.5%, compared with control lot. It appears that the cows from Romanian Spotted reacts better at GnRH treatment (44.8% conception rate), compared with Romanian Black Spotted (36.4 % conception rate). [ABSTRACT FROM AUTHOR]