1. Characterization of cerebrospinal fluid by comprehensive two-dimensional gas chromatography coupled to time-of-flight mass spectrometry.
- Author
-
Hartonen M, Mattila I, Ruskeepää AL, Orešič M, and Hyötyläinen T
- Subjects
- Aged, Amino Acids blood, Amino Acids chemistry, Carbohydrates blood, Carbohydrates chemistry, Female, Humans, Lipids blood, Lipids chemistry, Male, Reproducibility of Results, Amino Acids cerebrospinal fluid, Carbohydrates cerebrospinal fluid, Gas Chromatography-Mass Spectrometry methods, Gas Chromatography-Mass Spectrometry standards, Lipids cerebrospinal fluid, Metabolome
- Abstract
Comprehensive two-dimensional gas chromatography-time-of-flight mass spectrometry (GC×GC-TOFMS) was applied in the quantification and identification of organic compounds in patient-matched human cerebrospinal fluid (CSF) and serum samples. Concentrations of 21 amino and hydroxyl acids varied from 0.04 to 77ng/μl in CSF and from 0.1 to 84ng/μl in serum. In total, 91 metabolites out of over 1200 detected were identified based on mass spectra and retention indices. The other metabolites were identified at the functional group level. The main metabolites detected in CSF were sugar and amino acid derivatives. The CSF and serum had clearly distinct metabolic profiles, with larger biological variation in the serum than in CSF. The GC×GC-TOFMS allowed detection and identification of several metabolites that have not been previously detected in CSF., (Copyright © 2013 Elsevier B.V. All rights reserved.)
- Published
- 2013
- Full Text
- View/download PDF