22 results on '"Caries-free"'
Search Results
2. Empowering adolescent cadres for promoting oral health behavior: A persuasive communication training pilot study in Indonesia
- Author
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Arlette Suzy Setiawan, Naninda Berliana Pratidina, Arief Budiarto, and Susi Sukmasari
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cadre ,caries-free ,dental health behavior ,parents ,persuasive communication training ,Dentistry ,RK1-715 - Abstract
Background: Adolescents are considered good cadres to be able to socialize with this healthy dental behavior. Therefore, designing a dental health education program is necessary to prepare adolescents to socialize with dental health behavior. Purpose: This study evaluates the pilot training for trainers using persuasive communication to socialize dental health behavior. Methods: Thirty-five participants (13-16 years old) are trained by a persuasive communication coach and practice the skills with a simulated client”this one-day training has a two-phase structure; lectures and practicum. The evaluation is done through three out of four-level methods from Kirkpatrick (1959). The data analysis used for the reaction evaluation is descriptive statistics, calculating the average score per category of training support assessment items (curriculum, facilitator, accommodation, and overall score). The learning outcome analysis resulted from an analysis of the pre-post test results using the N-Gain value and paired T-test. Behavior outcomes were also analyzed descriptively by calculating the average score of all participants in every timeline (baseline and two weeks after the training). Results: evaluation of this training revealed that "reaction” has an average total score was 4.02, which shows that the participants were satisfied with the training, "learning” has an N-Gain acquisition of 48.28%, and "behavior” level shows a good outcome as an increased in points from 3.4 to 4.2. Conclusion: The training program produced thirty cadres ready to implement the program in the community.
- Published
- 2024
- Full Text
- View/download PDF
3. Empowering adolescent cadres for promoting oral health behavior: A persuasive communication training pilot study in Indonesia.
- Author
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Setiawan, Arlette Suzy, Pratidina, Naninda Berliana, Budiarto, Arief, and Sukmasari, Susi
- Subjects
HEALTH behavior ,DENTAL health education ,SELF-efficacy ,CURRICULUM evaluation ,ORAL health ,COMMUNITY-based programs - Abstract
Background: Adolescents are considered good cadres who can socialize healthy dental behavior. Therefore, designing a dental health education program is necessary to prepare adolescents to socialize dental health behavior. Purpose: This study evaluates the pilot program for trainers using persuasive communication to socialize dental health behavior. Methods: Thirty-five participants (13-16 years old) were trained by a persuasive communication coach and practiced the skills with a simulated client. This one-day training had a two-phase structure: lectures and practicum. The evaluation was completed utilizing three of the four-level methods from Kirkpatrick (1959). The data analysis used for the reaction evaluation was descriptive statistics, calculating the average score per category of training support assessment items (curriculum, facilitator, accommodation, and overall score). The learning outcome analysis resulted from an analysis of the pre- and post-test results using the N-Gain value and paired t-test. Behavior outcomes were also analyzed descriptively by calculating the average score of all participants in every timeline (baseline and two weeks after the training). Results: Evaluation of this training revealed that reaction has an average total score of 4.02, which shows that the participants were satisfied with the training, learning has an N-Gain acquisition of 48.28%, and behavior level shows a positive outcome as an increase in points from 3.4 to 4.2. Conclusion: The training program produced thirty cadres ready to implement the program in the community. [ABSTRACT FROM AUTHOR]
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- 2024
- Full Text
- View/download PDF
4. Dental Caries in Relation to Type of Disability: A Cross-sectional Study of Disabled Children in Tehran, Iran.
- Author
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Ghorbani, Zahra, Fathi, Homa, Alsheekhly, Bassam, Ghasemi, Hadi, Kazemi, Alireza, and Namdari, Mahshid
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CHILDREN with disabilities ,DISABILITIES ,PEOPLE with disabilities ,DENTAL caries ,AUTISM spectrum disorders - Abstract
Objectives Research indicates a high prevalence of dental caries and significant unmet dental treatment needs among individuals with disabilities. This study aimed to evaluate the oral health status of disabled students in Tehran, Iran, considering factors such as age, gender, and type of disability. Methods The study involved 1,170 disabled students aged 6 to 20 years, each with one or more of the following disabilities: physical retardation (PR), hearing impairment (HI), visual impairment (VI), mental retardation (MR), and autism spectrum disorder (ASD). The mean decayed, missing, and filled teeth index was used as an oral health indicator (dmft for children aged 6- 12 years, and DMFT for children older than 9 years). Mann-Whitney and Kruskal-Wallis tests at significant level of 0.05 were conducted for comparisons. Results Mental retardation (MR) was the most prevalent disability (59.4%). The decayed component constituted the largest part of the mean dmft (82%) and DMFT (75%) indices in all age groups. Girls had significantly higher DMFT scores compared to boys (P < 0.001). Among the different disabilities, children with HI had the highest dmft score (mean ± SD = 2.17 ± 2.86), while those with MR had the highest DMFT score (mean ± SD = 3.76 ± 3.83). Additionally, the caries-free ratio was significantly higher among VI students in the 9-12 years (40.5%) and older than 13 years (38.8%) age groups. Conclusion The dmft/DMFT scores were elevated among disabled children, yet they remained lower than those of healthy children. Dental caries were more common in girls. Although children with VI had lower dmft/DMFT scores, those with HI had the highest dmft scores, and those with MR had the highest DMFT scores. [ABSTRACT FROM AUTHOR]
- Published
- 2024
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5. High-throughput sequencing identifies salivary microbiota in Chinese caries-free preschool children with primary dentition.
- Author
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Xu, Lei, Wu, Zhifang, Wang, Yuan, Wang, Sa, Shu, Chang, Duan, Zhuhui, and Deng, Shuli
- Abstract
Copyright of Journal of Zhejiang University: Science B is the property of Springer Nature and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
- Published
- 2021
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- View/download PDF
6. Proteomic and bioinformatics analysis of human saliva for the dental-risk assessment
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Laputková Galina, Bencková Mária, Alexovič Michal, Schwartzová Vladimíra, Talian Ivan, and Sabo Ján
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saliva ,proteins ,caries-susceptible ,caries-free ,proteomic analysis ,bioinformatics ,Biology (General) ,QH301-705.5 - Abstract
Background: Dental caries disease is a dynamic process with a multi-factorial etiology. It is manifested by demineralization of enamel followed by damage spreading into the tooth inner structure. Successful early diagnosis could identify caries-risk and improve dental screening, providing a baseline for evaluating personalized dental treatment and prevention strategies. Methodology: Salivary proteome of the whole unstimulated saliva (WUS) samples was assessed in caries-free and caries-susceptible individuals of older adolescent age with permanent dentition using a nano-HPLC and MALDI-TOF/TOF mass spectrometry. Results: 554 proteins in the caries-free and 695 proteins in the caries-susceptible group were identified. Assessment using bioinformatics tools and Gene Ontology (GO) term enrichment analysis revealed qualitative differences between these two proteomes. Members of the caries-susceptible group exhibited a branch of cytokine binding gene products responsible for the regulation of immune and inflammatory responses to infections. Inspection of molecular functions and biological processes of caries-susceptible saliva samples revealed significant categories predominantly related to the activity of proteolytic peptidases, and the regulation of metabolic and catabolic processes of carbohydrates. Conclusions: Proteomic analysis of the whole saliva revealed information about potential risk factors associated with the development of caries-susceptibility and provides a better understanding of tooth protection mechanisms.
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- 2017
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7. Caries-related factors and bacterial composition of supragingival plaques in caries free and caries active Algerian adults
- Author
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Amina Hoceini, Nihel Klouche Khelil, Ilhem Ben-Yelles, Amine Mesli, Sara Ziouani, Lotfi Ghellai, Nadia Aissaoui, Fatima Nas, and Mounia Arab
- Subjects
Supragingival plaque ,Caries-free ,Caries-active ,Bacterial composition ,Algerian adults ,Cultural level ,Food intake ,Arctic medicine. Tropical medicine ,RC955-962 ,Biology (General) ,QH301-705.5 - Abstract
Objective: To compare oral hygiene practices, education and social background, food intake and oral malodor of Algerian adults suffering from dental caries with normal controls, and to determine and compare the bacterial composition of the supragingival plaques from the above-mentioned groups. Methods: Participants completed a questionnaire and were clinically examined for dental caries using decayed, missing and filled teeth index according to the criteria laid down by the World Health Organization. Supragingival plaque samples were collected from 50 caries-free adults (CF) and 50 caries-active adults (CA). Standard procedures of culture and identification of aerobic and anaerobic bacteria were used. Data were analyzed using Chi-square test. Results: A total of 117 bacterial strains were isolated from supragingival plaques in CF group subjects, 76 (64.96%) of them belonged to 9 aerobic genera, and 41 (35.04%) to 9 anaerobic genera (P
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- 2016
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8. Evaluation of pH, buffering capacity, viscosity and flow rate levels of saliva in caries-free, minimal caries and nursing caries children: An in vivo study
- Author
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Dwitha Animireddy, Venkata Thimma Reddy Bekkem, Pranitha Vallala, Sunil Babu Kotha, Swetha Ankireddy, and Noorjahan Mohammad
- Subjects
Caries-free ,minimal-caries ,nursing-caries ,salivary buffering capacity ,salivary flow rate ,salivary pH ,salivary viscosity ,Dentistry ,RK1-715 - Abstract
Background and Aim: The present study was undertaken to evaluate the pH, buffering capacity, viscosity and flow rate of saliva in caries free, minimal caries and nursing caries children and to evaluate the relationship of these on the caries activity of children. Materials and Methods: A total of 75 school children of age group between 4 and 12 years were selected and divided into three equal groups: Group I, Group II and Group III, consisting of 25 subjects each. Group I included caries-free subjects, Group II included subjects with minimal caries and Group III included subjects with nursing caries. Saliva samples were collected from all subjects and were estimated for flow rate, pH, buffering capacity and viscosity. Results: There was a significant decrease in the mean salivary flow rate, salivary ph and salivary buffer capacity and a significant increase in the salivary viscosity among caries-free subjects, subjects with minimal caries and subjects with nursing caries. Conclusion: The physicochemical properties of saliva, such as salivary flow rate, pH, buffering capacity and viscosity, has a relation with caries activity in children and act as markers of caries activity.
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- 2014
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9. Evaluation of non-microbial salivary caries activity parameters and salivary biochemical indicators in predicting dental caries
- Author
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A Kaur, K S Kwatra, and P Kamboj
- Subjects
Alkaline phosphatase ,buffering capacity ,calcium ,caries-active ,caries-free ,flow rate ,level of hydration ,pH ,phosphorus ,relative viscosity ,Dentistry ,RK1-715 - Abstract
Aim: The aim of the present study was the evaluation of non-microbial salivary caries activity parameters and salivary biochemical indicators in predicting dental caries. Materials and Methods: The present study was carried out on 60 children, aged 4-6 years, selected from the schools of Panchkula district, Haryana, on the basis of their caries status. Level of hydration, flow rate, pH, buffering capacity, relative viscosity, calcium, phosphorus and alkaline phosphatase levels in caries-free and caries-active children were evaluated. Results: Results showed that 90% of subjects in the caries-free group and 30% of subjects in the caries-active group had normal level of hydration value of less than 60 s and the difference was found to be statistically very highly significant. Normal flow rate of stimulated saliva was found in 90% of the subjects in caries-free group and 33.3% subjects in the caries active group and difference was found to be statistically very highly significant. Adequate salivary pH was found in 100% subjects in caries-free group and 30% in caries-active group and the difference was statistically very highly significant. Conclusion: To conclude, within limitations of this study, it became clear that normal level of hydration and higher values for flow rate, pH, buffering capacity of saliva lead to good oral health and a reduced caries occurrence. Increased salivary viscosity plays a role in increasing caries incidence. Salivary biochemical indicators like calcium, phosphorus and alkaline phosphatase also play their respective role in determining caries susceptibility of an individual. These salivary parameters can be used as diagnostic tool for caries risk assessment.
- Published
- 2012
- Full Text
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10. Caries-related factors and bacterial composition of supragingival plaques in caries free and caries active Algerian adults.
- Author
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Hoceini, Amina, Khelil, Nihel Klouche, Ben-Yelles, Ilhem, Mesli, Amine, Ziouani, Sara, Ghellai, Lotfi, Aissaoui, Nadia, Nas, Fatima, and Arab, Mounia
- Abstract
Objective: To compare oral hygiene practices, education and social background, food intake and oral malodor of Algerian adults suffering from dental caries with normal controls, and to determine and compare the bacterial composition of the supragingival plaques from the above-mentioned groups. Methods: Participants completed a questionnaire and were clinically examined for dental caries using decayed, missing and filled teeth index according to the criteria laid down by the World Health Organization. Supragingival plaque samples were collected from 50 caries-free adults (CF) and 50 caries-active adults (CA). Standard procedures of culture and identification of aerobic and anaerobic bacteria were used. Data were analyzed using Chi-square test. Results: A total of 117 bacterial strains were isolated from supragingival plaques in CF group subjects, 76 (64.96%) of them belonged to 9 aerobic genera, and 41 (35.04%) to 9 anaerobic genera (P < 0.05). While in the second group, 199 strains were isolated, 119 (59.80%) of the strains belonged to 10 aerobic genera and 80 (40.20%) to 10 anaerobic bacteria (P < 0.05). Streptococcus mutans, Enterococcus faecium, Aerococcus viridans, Actinomyces meyeri, Lactobacillus acidophilus and Eubacterium limosum showed a significantly higher prevalence in the CA group (P < 0.05). The findings revealed that CA group had a high sugar intake (80%). A significantly higher frequency of tooth brushing (P < 0.000) and a significantly less self-reported oral malodor (P < 0.000) and tooth pain (P < 0.000) were found in CF group, while there was no association of socioeconomic levels and intake of meal snacks with dental caries. Conclusions: This study confirms the association of some aciduric bacteria with caries formation, and a direct association of sugar intake and cultural level with dental caries. Furthermore, oral hygiene practices minimize the prevalence of tooth decay. [ABSTRACT FROM AUTHOR]
- Published
- 2016
- Full Text
- View/download PDF
11. Evaluation of pH, buffering capacity, viscosity and flow rate levels of saliva in caries-free, minimal caries and nursing caries children: An in vivo study.
- Author
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ANIMIREDDY, DWITHA, REDDY BEKKEM, VENKATA THIMMA, VALLALA, PRANITHA, BABU KOTHA, SUNIL, ANKIREDDY, SWETHA, and MOHAMMAD, NOORJAHAN
- Abstract
Background and Aim: The present study was undertaken to evaluate the pH, buffering capacity, viscosity and flow rate of saliva in caries free, minimal caries and nursing caries children and to evaluate the relationship of these on the caries activity of children. Materials and Methods: A total of 75 school children of age group between 4 and 12 years were selected and divided into three equal groups: Group I, Group II and Group III, consisting of 25 subjects each. Group I included caries-free subjects, Group II included subjects with minimal caries and Group III included subjects with nursing caries. Saliva samples were collected from all subjects and were estimated for flow rate, pH, buffering capacity and viscosity. Results: There was a significant decrease in the mean salivary flow rate, salivary ph and salivary buffer capacity and a significant increase in the salivary viscosity among caries-free subjects, subjects with minimal caries and subjects with nursing caries. Conclusion: The physicochemical properties of saliva, such as salivary flow rate, pH, buffering capacity and viscosity, has a relation with caries activity in children and act as markers of caries activity. [ABSTRACT FROM AUTHOR]
- Published
- 2014
- Full Text
- View/download PDF
12. Caries Resistance as a Function of Age in an Initially Caries-free Population.
- Author
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Wen, A., Goldberg, D., Marrs, C.F., Weyant, R.J., Marazita, M.L., Srinivasan, U., Zhang, L., Crout, R., McNeil, D.W., and Foxman, B.
- Subjects
DENTAL caries in children ,DENTAL caries research ,BAYESIAN analysis ,EPIDEMIOLOGY ,DENTAL public health ,STATISTICAL correlation ,MATHEMATICAL models - Abstract
Using data from the Center for Oral Health Research in Appalachia Study, we examined variability in susceptibility to dental caries among children and adolescents in rural Appalachia. Among 210 participants who were caries-free at the initial visit, age at the baseline visit can be used as a proxy for the degree of caries resistance; probability of caries development at the tooth level decreased as age at the baseline visit increased. Participants who stayed caries-free for a longer period during childhood and adolescence experienced less extensive caries, as measured by the number of carious teeth. However, the probability of becoming caries-positive did not correlate with age at the baseline visit. For children between 1 and 18 years of age, there was not a “threshold age” after which a caries-free child’s risk of caries onset is significantly reduced. [ABSTRACT FROM PUBLISHER]
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- 2012
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- View/download PDF
13. Evaluation of non-microbial salivary caries activity parameters and salivary biochemical indicators in predicting dental caries.
- Author
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Kaur, A., Kwatra, K. S., and Kamboj, P.
- Subjects
DENTAL caries risk factors ,ALKALINE phosphatase ,DENTAL pathology ,PARAMETER estimation ,DENTAL materials ,MEDICAL statistics - Abstract
Aim: The aim of the present study was the evaluation of non-microbial salivary caries activity parameters and salivary biochemical indicators in predicting dental caries. Materials and Methods: The present study was carried out on 60 children, aged 4-6 years, selected from the schools of Panchkula district, Haryana, on the basis of their caries status. Level of hydration, flow rate, pH, buffering capacity, relative viscosity, calcium, phosphorus and alkaline phosphatase levels in caries-free and caries-active children were evaluated. Results: Results showed that 90% of subjects in the caries-free group and 30% of subjects in the caries-active group had normal level of hydration value of less than 60 s and the difference was found to be statistically very highly significant. Normal flow rate of stimulated saliva was found in 90% of the subjects in caries-free group and 33.3% subjects in the caries active group and difference was found to be statistically very highly significant. Adequate salivary pH was found in 100% subjects in caries-free group and 30% in caries-active group and the difference was statistically very highly significant. Conclusion: To conclude, within limitations of this study, it became clear that normal level of hydration and higher values for flow rate, pH, buffering capacity of saliva lead to good oral health and a reduced caries occurrence. Increased salivary viscosity plays a role in increasing caries incidence. Salivary biochemical indicators like calcium, phosphorus and alkaline phosphatase also play their respective role in determining caries susceptibility of an individual. These salivary parameters can be used as diagnostic tool for caries risk assessment. [ABSTRACT FROM AUTHOR]
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- 2012
- Full Text
- View/download PDF
14. Dental caries in preschoolers from communes with fluoridated and non-fluoridated public water supplies in Chile.
- Author
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Lopez, Ismael Yévenes, Hernández, Barbara, Ramos, Alfredo Apip, Jara, Miguel Neira, Wolfenson, Paula Maass, and Smith, Ljubica Petrasic
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CROSS-sectional method ,DENTAL caries in children ,WATER fluoridation ,PEDIATRIC dentistry ,FLUORIDES ,KINDERGARTEN ,WATER supply - Abstract
Copyright of Revista Odonto Ciencia is the property of EDIPUCRS - Editora Universitaria da PUCRS and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
- Published
- 2010
- Full Text
- View/download PDF
15. Prevalence of dental caries in preschool children in Peñaflor, Santiago, Chile.
- Author
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López, Ismael Yévenes, Bustos, Bianca Campos, Ramos, Alfredo Apip, Espinoza, Ronald Moztfeld, Jara, Miguel Neira, and Smith, Ljubica Petrasic
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DENTAL caries in children ,DENTAL pathology ,DECIDUOUS teeth ,CHILDREN'S health - Abstract
Copyright of Revista Odonto Ciencia is the property of EDIPUCRS - Editora Universitaria da PUCRS and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
- Published
- 2009
16. Characterization of caries-free young
- Author
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Lopes, Mayara Marin [Unesp], Universidade Estadual Paulista (Unesp), and Teixeira, Symone Cristina [UNESP]
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Habits ,Jovens ,Hábitos ,Livre de cárie ,Cárie dentária ,Young people ,Dental cavity ,Caries-free ,Classe Social ,Social class - Abstract
Submitted by Mayara Marin Lopes (mayara.lopes@unesp.br) on 2019-09-18T14:24:09Z No. of bitstreams: 1 DISSERTAÇÃO MESTRADO PROFISSIONAL - MAYARA MARIN LOPES corrigido 16.09 com folha de rosto (1).pdf: 1404588 bytes, checksum: 987ed73e09d1053d8c9bd3610a996194 (MD5) Approved for entry into archive by Silvana Alvarez null (silvana@ict.unesp.br) on 2019-09-24T14:14:47Z (GMT) No. of bitstreams: 1 lopes_mm_me_sjc.pdf: 1404588 bytes, checksum: 987ed73e09d1053d8c9bd3610a996194 (MD5) Made available in DSpace on 2019-09-24T14:14:47Z (GMT). No. of bitstreams: 1 lopes_mm_me_sjc.pdf: 1404588 bytes, checksum: 987ed73e09d1053d8c9bd3610a996194 (MD5) Previous issue date: 2019-08-27 O trabalho teve como objetivo caracterizar o perfil socioeconômico e hábitos alimentares e de higiene de jovens, com idade entre 18 e 24 anos, livres de cárie, beneficiando a odontologia no sentido de estudar dados relacionados à saúde e não a doença como já bem relatado na literatura. Através de exame clínico simples foram selecionados os alunos livres de cárie (88) que então responderam a um questionário com questões sobre o perfil socioeconômico da família, os hábitos alimentares, de higiene, de rotina ao consultório odontológico e incentivo dos responsáveis da infância até os dias atuais. O perfil socioeconômico mais prevalente foi entre os estratos A a B2, com o nível de escolaridade dos pais entre médio completo e pós-graduação. Em relação à água fluoretada e a informações sobre saúde bucal 93% responderam ter tido acesso. As escovações foram relatadas no mínimo de 1 a 3 vezes por dia, chegando até a 5 vezes. Os consultórios particulares foram os mais visitados, com um intervalo de 3 a 6 meses entre as consultas por motivo de prevenção. A escovação foi supervisionada pelos pais até os 7 anos de idade por 63,64% dos entrevistados. Desta forma, os dados colhidos durante esta pesquisa podem auxiliar na formação, reorientação ou adaptação de políticas públicas de saúde através da organização do serviço, planejamento de estratégias para os trabalhos principalmente de educação, atenção e prevenção em saúde bucal, trazendo benefícios não só a odontologia, mas à toda população. The objective of this study was to characterize the socioeconomic profile and eating and hygiene habits of young people., aged 18 to 24 years old, caries-free, benefiting dentistry in order to study data related to health and not the disease as already reported in the literature. Through a simple clinical examination, caries-free students were selected (88), who then answered a questionnaire with questions about the family's socioeconomic profile, eating habits, hygiene, routine dental appointments, and indulcement of parents or guardians for childhood to the present day. The most prevalent socioeconomic profile was between the strata A and B2, with the level of schooling of the parents between full middle and postgraduate. Regarding fluoridated water and oral health information, 93% said they had access. Brushing has been reported at least 1 to 3 times a day, up to 5 times. Private practices were the most visited, with a 3-6 month interval between consultations due to prevention. Brushing was supervised by parents up to 7 years of age by 63.64% of the interviewees. Therefore, the data collected during this research can help in the formation, reorientation or adaptation of public health policies through the organization of the service, planning strategies for the work mainly education, attention and prevention in oral health, bringing benefits not only dentistry, but the entire population.
- Published
- 2019
17. Effects of Caries Activity on Compositions of Mutans Streptococci in Saliva-Induced Biofilm Formed on Bracket Materials
- Author
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Sug-Joon Ahn, Bum-Soon Lim, Bo-Hyun Kim, and Won-Jun Shon
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caries-free ,Saliva ,Article ,Streptococcus sobrinus ,Microbiology ,03 medical and health sciences ,fluids and secretions ,0302 clinical medicine ,stomatognathic system ,caries-active ,General Materials Science ,030304 developmental biology ,0303 health sciences ,biology ,Chemistry ,Bracket ,Biofilm ,mutans streptococci ,030206 dentistry ,Adhesion ,biochemical phenomena, metabolism, and nutrition ,orthodontic bracket ,Caries activity ,biology.organism_classification ,Streptococcus mutans ,stomatognathic diseases ,caries activity ,Bacteria - Abstract
This study aimed to investigate effects of caries activity on composition of mutans streptococci in saliva-induced biofilms formed on bracket materials. Three bracket materials were used as specimens: ceramic, metal, and plastic. After saliva was collected using a spitting method from caries-active (CA, decayed, missing, filled teeth (DMFT) score ≥ 10) and caries-free (CF, DMFT score = 0) subjects, saliva was mixed with growth media in a proportion of 1:10. The saliva solution was then incubated with each bracket material. After a saliva-induced biofilm was developed on the surface of the bracket material, the amounts of total bacteria and mutans streptococci were determined using real-time polymerase chain reaction. The results showed that biofilms from CA saliva contained more mutans streptococci but less total bacteria than biofilms from CF saliva, regardless of material type. Adhesion of total bacteria to ceramic was higher than to plastic, regardless of caries activity. Mutans streptococci adhered more to ceramic than to metal and plastic in both biofilms from CA and CF saliva, but there was a difference in adhesion between Streptococcus mutans and Streptococcus sobrinus. The amount of S. mutans was higher than that of S. sobrinus in biofilms from CA saliva despite similar amounts of the two strains in biofilms from CF saliva. The stronger adhesion of S. mutans to ceramic than to metal and plastic was more evident in biofilms from CA saliva than in biofilms from CF saliva. This study suggests that caries activity and material type significantly influenced composition of mutans streptococci in biofilms formed on bracket materials.
- Published
- 2020
- Full Text
- View/download PDF
18. The Prevalence of Early Childhood Caries among 24 to 36 Months Old Children of Iran: Using the Novel ICDAS-II Method
- Author
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Toutouni, Hediyeh, Nokhostin, Mohammad-Reza, Amaechi, Bennett Tochukwu, and Zafarmand, Abdol Hamid
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Oral health ,ICDAS-II ,Young children ,Dental caries ,Prevalence ,Original Article ,Caries-free ,Iran ,Early childhood caries - Abstract
Statement of the Problem Early childhood caries is an important oral health issue. Finding its prevalence would predict the need for oral health promotion disciplines for specific age groups. Purpose The aim of this study was to assess the caries experience of children living in Tehran, Iran. It also would evaluate the impact of gender, ethnicity, and socioeconomic status (SES) on this oral condition. Materials and Method This epidemiological cross-sectional study was based upon stratified cluster random sampling. The samples consisted of 239 children (2- to 3- years old) registered in Tehran’s public healthcare centers for “Healthy Child Program”. Mothers of the recruited children were interviewed for the background data; then children were examined for the oral health status according to ICDAS-II (International Caries Detection and Assessment System) and WHO (World Health Organization) criteria. Statistical analyses were conducted using STATA.11 for SES classification considering six socioeconomic variables, and SPSS.21 for descriptive/analytical analyses. Results Primary Component Analysis (PCA) demonstrated five classes of SES ranging from the lowest to the highest. The distribution of caries-free (CF) children was 10.87%, non-cavitated enamel caries (codes 01-02) were 28.03%, and about 61.1% had cavitated caries (codes 03-06). There was no significant difference in caries experience between the two genders. Cavitated lesions were more prevalent among Kurdish, who also had the least CF children. Caries prevalence, especially code 02, was more among children from 3rd class SES (moderate level). Gender, ethnicity, or SES had no impact on the CF status of the children; however, ethnicity showed significant impact on the prevalence of extensive caries (codes 05-06). Conclusion The result of the present study is indicative of high caries prevalence among 2 to 3 years old children residing in Tehran. It highlights the need for comprehensive oral health promotion disciplines for this age group.
- Published
- 2015
19. Effects of Caries Activity on Compositions of Mutans Streptococci in Saliva-Induced Biofilm Formed on Bracket Materials.
- Author
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Lim, Bum-Soon, Kim, Bo-Hyun, Shon, Won-Jun, and Ahn, Sug-Joon
- Subjects
STREPTOCOCCUS mutans ,BIOFILMS ,CERAMIC metals ,BACTERIAL adhesion ,POLYMERASE chain reaction ,BRACKETS - Abstract
This study aimed to investigate effects of caries activity on composition of mutans streptococci in saliva-induced biofilms formed on bracket materials. Three bracket materials were used as specimens: ceramic, metal, and plastic. After saliva was collected using a spitting method from caries-active (CA, decayed, missing, filled teeth (DMFT) score ≥ 10) and caries-free (CF, DMFT score = 0) subjects, saliva was mixed with growth media in a proportion of 1:10. The saliva solution was then incubated with each bracket material. After a saliva-induced biofilm was developed on the surface of the bracket material, the amounts of total bacteria and mutans streptococci were determined using real-time polymerase chain reaction. The results showed that biofilms from CA saliva contained more mutans streptococci but less total bacteria than biofilms from CF saliva, regardless of material type. Adhesion of total bacteria to ceramic was higher than to plastic, regardless of caries activity. Mutans streptococci adhered more to ceramic than to metal and plastic in both biofilms from CA and CF saliva, but there was a difference in adhesion between Streptococcus mutans and Streptococcus sobrinus. The amount of S. mutans was higher than that of S. sobrinus in biofilms from CA saliva despite similar amounts of the two strains in biofilms from CF saliva. The stronger adhesion of S. mutans to ceramic than to metal and plastic was more evident in biofilms from CA saliva than in biofilms from CF saliva. This study suggests that caries activity and material type significantly influenced composition of mutans streptococci in biofilms formed on bracket materials. [ABSTRACT FROM AUTHOR]
- Published
- 2020
- Full Text
- View/download PDF
20. Phenotypic Heterogeneity of Genomically-Diverse Isolates of Streptococcus mutans
- Author
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Tristan Lefébure, Robert A. Burne, Vincent P. Richards, Lin Zeng, José A. Lemos, James H. Miller, Michael J. Stanhope, Jacqueline Abranches, Sara R. Palmer, Department of Oral Biology, University of Florida [Gainesville], Center for Oral Biology, University of Rochester Medical Center, Department of Microbiology and Immunology, Écologie, Évolution, Écosystemes Souterrains, Institut Universitaire de France (IUF), Ministère de l'Education nationale, de l’Enseignement supérieur et de la Recherche (M.E.N.E.S.R.)-Ministère de l'Education nationale, de l’Enseignement supérieur et de la Recherche (M.E.N.E.S.R.)-Laboratoire d'Ecologie des Hydrosystèmes Naturels et Anthropisés (LEHNA), Centre National de la Recherche Scientifique (CNRS)-Institut National de la Recherche Agronomique (INRA)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-École Nationale des Travaux Publics de l'État (ENTPE)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Recherche Agronomique (INRA)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-École Nationale des Travaux Publics de l'État (ENTPE), Department of Population Medicine and Diagnostic Sciences, College of Veterinary Medicine, IN2P3, University of Florida [Gainesville] (UF), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-École Nationale des Travaux Publics de l'État (ENTPE)-Centre National de la Recherche Scientifique (CNRS)-Université Claude Bernard Lyon 1 (UCBL), and Université de Lyon-Université de Lyon-École Nationale des Travaux Publics de l'État (ENTPE)-Centre National de la Recherche Scientifique (CNRS)
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virulence traits ,lcsh:Medicine ,Evolutionary Selection ,Genome ,Streptococcus mutans ,stress ,Oral Diseases ,oxidative ,Bacterial Physiology ,ORFS ,lcsh:Science ,Genetics ,0303 health sciences ,Multidisciplinary ,biology ,Shotgun sequencing ,Microbial Growth and Development ,Streptococci ,Genomics ,Bacterial Pathogens ,collagen-binding protein ,Medicine ,Research Article ,caries-free ,Evolutionary Processes ,Sequence analysis ,Oral Medicine ,bacteriocin production ,artery endothelial-cells ,Virulence ,system ,Microbiology ,quorum-sensing ,03 medical and health sciences ,Bacteriocin ,Bacterial Proteins ,Gene ,Biology ,Microbial Pathogens ,030304 developmental biology ,competence development ,Evolutionary Biology ,Gram Positive ,Bacterial Evolution ,stress tolerance ,030306 microbiology ,lcsh:R ,Bacteriology ,Comparative Genomics ,biology.organism_classification ,Organismal Evolution ,Microbial Evolution ,biofilm formation ,lcsh:Q ,[SDE.BE]Environmental Sciences/Biodiversity and Ecology ,Bacterial Biofilms ,Genome, Bacterial - Abstract
International audience; High coverage, whole genome shotgun (WGS) sequencing of 57 geographically- and genetically-diverse isolates of Streptococcus mutans from individuals of known dental caries status was recently completed. Of the 57 sequenced strains, fifteen isolates, were selected based primarily on differences in gene content and phenotypic characteristics known to affect virulence and compared with the reference strain UA159. A high degree of variability in these properties was observed between strains, with a broad spectrum of sensitivities to low pH, oxidative stress (air and paraquat) and exposure to competence stimulating peptide (CSP). Significant differences in autolytic behavior and in biofilm development in glucose or sucrose were also observed. Natural genetic competence varied among isolates, and this was correlated to the presence or absence of competence genes, comCDE and comX, and to bacteriocins. In general strains that lacked the ability to become competent possessed fewer genes for bacteriocins and immunity proteins or contained polymorphic variants of these genes. WGS sequence analysis of the pan-genome revealed, for the first time, components of a Type VII secretion system in several S. mutans strains, as well as two putative ORFs that encode possible collagen binding proteins located upstream of the cnm gene, which is associated with host cell invasiveness. The virulence of these particular strains was assessed in a wax-worm model. This is the first study to combine a comprehensive analysis of key virulence-related phenotypes with extensive genomic analysis of a pathogen that evolved closely with humans. Our analysis highlights the phenotypic diversity of S. mutans isolates and indicates that the species has evolved a variety of adaptive strategies to persist in the human oral cavity and, when conditions are favorable, to initiate disease.
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- 2013
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21. Evaluation of non-microbial salivary caries activity parameters and salivary biochemical indicators in predicting dental caries
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KS Kwatra, A Kaur, and P Kamboj
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caries-free ,Saliva ,Dental Caries Susceptibility ,Dentistry ,buffering capacity ,Oral health ,Buffers ,Dental Caries ,level of hydration ,Risk Assessment ,Caries active ,caries-active ,Medicine ,Humans ,Child ,General Dentistry ,relative viscosity ,Caries susceptibility ,pH ,business.industry ,DMF Index ,Viscosity ,Normal level ,Phosphorus ,Hydrogen-Ion Concentration ,Caries activity ,Alkaline Phosphatase ,lcsh:RK1-715 ,lcsh:Dentistry ,Child, Preschool ,Alkaline phosphatase ,Calcium ,flow rate ,business ,Secretory Rate ,Forecasting - Abstract
Aim: The aim of the present study was the evaluation of non-microbial salivary caries activity parameters and salivary biochemical indicators in predicting dental caries. Materials and Methods: The present study was carried out on 60 children, aged 4-6 years, selected from the schools of Panchkula district, Haryana, on the basis of their caries status. Level of hydration, flow rate, pH, buffering capacity, relative viscosity, calcium, phosphorus and alkaline phosphatase levels in caries-free and caries-active children were evaluated. Results: Results showed that 90% of subjects in the caries-free group and 30% of subjects in the caries-active group had normal level of hydration value of less than 60 s and the difference was found to be statistically very highly significant. Normal flow rate of stimulated saliva was found in 90% of the subjects in caries-free group and 33.3% subjects in the caries active group and difference was found to be statistically very highly significant. Adequate salivary pH was found in 100% subjects in caries-free group and 30% in caries-active group and the difference was statistically very highly significant. Conclusion: To conclude, within limitations of this study, it became clear that normal level of hydration and higher values for flow rate, pH, buffering capacity of saliva lead to good oral health and a reduced caries occurrence. Increased salivary viscosity plays a role in increasing caries incidence. Salivary biochemical indicators like calcium, phosphorus and alkaline phosphatase also play their respective role in determining caries susceptibility of an individual. These salivary parameters can be used as diagnostic tool for caries risk assessment.
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- 2012
22. Dental caries in preschoolers from communes with fluoridated and non-fluoridated public water supplies in Chile
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Ljubica Petrasic Smith, Miguel Neira Jara, Barbara Hernández, Paula Maass Wolfenson, Ismael Yevenes López, and Alfredo Apip Ramos
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caries-free ,preschool children ,fluoride ,business.industry ,DMFT Index ,Cárie dentária ,Dentistry ,Water supply ,flúor ,World health ,pré-escolares ,Environmental health ,parasitic diseases ,Dental caries ,índice cpod ,Medicine ,General health ,business ,General Dentistry ,Socioeconomic status ,dmft index - Abstract
PURPOSE: To determine the prevalence and severity of dental caries in three- to five-year-old preschool children from two communes in Chile: one with a fluoridated public water supply and the other with a non-fluoridated public water supply. METHODS: Study design: Cross-sectional observational study. The sample was composed of 200 children from three to five years old, with good general health, of low socioeconomic status, attending four kindergarten schools of the Metropolitan Region of Santiago, Chile. Two schools were located in the commune of Maipú, which does not have fluoridated public water, and two schools were located in the commune of Peñalolen, which has had fluoridated public water since 1996, at a fluoride concentration of 0.6 mg/L. The WHO (World Health Organization) methodology was used to measure the percentage of caries-free children, and the dmft index was used to measure the severity of the disease. RESULTS: The results showed that 24% of the children in Maipú were caries free, compared to 55% in Peñalolen. The dmft index was 3.38 and 1.84 in Maipú and Peñalolen, respectively. These indices increased with age. CONCLUSION: The prevalence and severity of dental caries in three- to five-year-old preschoolers were higher in Maipú, the commune with non-fluoridated water, than in Peñalolen, the commune with fluoridated water. OBJETIVO: Determinar a prevalência e severidade da doença cárie dentária em crianças pré-escolares de 3 a 5 anos de idade em creches no Chile, localizadas em uma comunidade sem fluoretação da água de abastecimento público e em outra comunidade com agua fluoretada. METODOLOGIA: Delineamento do estudo: observacional, transversal. A amostra foi composta por 200 crianças de 3 a 5 anos, com boa saúde geral, pertencentes a baixos estratos sociais, de quatro creches da Região Metropolitana de Santiago, dois do município de Maipú sem fluoretação da água de abastecimento público e duas do município de Peñalolén com fluoretação desde 1996, com concentração de flúor de 0,6 mg/L. A metodologia da OMS foi utilizada para o registro da percentagem de crianças livres de cárie e o índice cpod foi usado para medir a severidade da doença. RESULTADOS: A porcentagem de crianças livres de cárie foi de 24% em Maipú e 55% em Peñalolén, com índice cpod de 3,38 e 1,84, respectivamente. Estes resultados aumentaram com a idade. CONCLUSÃO: A prevalência e severidade da cárie dentária em pré-escolares de 3 a 5 anos em Maipú (agua não fluoretada) foram superiores aos observados em Peñalolén (água fluoretada).
- Published
- 2010
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