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The objective of this research was to evaluate the effect of sperm on chromatin stability and its relationship with the membrane integrity structural – physiological and the rate of fertilization of female sheep. Ejaculates of sperm (2 × 10 9 sperm·mL -1 ) with 70% of motility were collected using an artificial vagina (n=5, 2 years old. For this, each ram was served with fifteen female sheep (n=75), generating thus five different Groups (A, B, C, D, and E). A control Group also was considered. Sperm nuclear chromatin stability (NCS) was evaluated using the Borate Buffer (BB), Sodium Dodecyl Sulfate (SDS), and the mixture of Ethylenediaminetetraacetic acid (EDTA) + SDS. The fertilization rate was evaluated after 16-18 hours post sperm injection. Sperm concentration showed a significant difference (P0.05). A high correlation (r 2 =0.52) was observed between morphology and motility, and the fertilization rate was 74.6% (n=56). It was concluded in general that techniques to evaluate nuclear condensation values do have a high likelihood to give a diagnosis about the future potential of sperm populations in Junín ram. El objetivo de esta investigación fue evaluar el efecto de los espermatozoides sobre la estabilidad de la cromatina y su relación con la integridad de la membrana estructural – fisiológica y la tasa de fertilización de las ovejas hembras. Las eyaculaciones de espermatozoides (2 × 10 9 espermatozoides·ml -1 ) con un 70 % de motilidad se recogieron mediante una vagina artificial (n=5, 2 años). Para ello, cada carnero se servía con quince ovejas hembra (n=75), generando así cinco grupos diferentes (A, B, C, D y E). También se consideró un grupo de control. La estabilidad de la cromatina nuclear (NCS) de los espermatozoides se evaluó utilizando el tampón de borato (BB), el dodecil sulfato de sodio (SDS) y la mezcla de ácido etilendiaminotetraacético (EDTA) + SDS. La tasa de fertilización se evaluó después de 16-18 horas después de la inyección de espermatozoides. La concentración de espermatozoides mostró diferencias significativas (P0,05). Se observó una alta correlación (r 2 =0,52) entre morfología y motilidad, y la tasa de fecundación fue del 74,6% (n=56). Se concluyó en general que las técnicas para evaluar los valores de condensación nuclear tienen una alta probabilidad de dar un diagnóstico sobre el potencial futuro de las poblaciones de espermatozoides en carneros de Junín. ide_85@hotmail.com

The objective of this study was to investigate the immunocastration using against gonadotropin-releasing factor (GnRF) with Bopriva® (Zoetis) on the performance of production variables, meat quality, docility, and scrotal circumference in young rams, from Sheep production center “Tupac Amaru”, Junin region, Perú. A total of 60 animals were divided into four treatments: T1, T2, and T3 with 0.50 mL, 0.75 mL, and 1.0 mL of doses with Bopriva®, respectively, and T4 (control - 1.0 mL of placebo (distilled water)), with fifteen animals in each. The variables were measured every 15 days for three months (September to November 2018). Datasets were assessed by one-way ANOVA and subsequent posthoc Tukey´s test to detect significant differences among treatments. The results indicated differences found between treatments with the immunocastration compared with non-castrated animals for final weight, carcass weight and performance, scrotal circumference, and docility. Growth performance and meat quality characteristics were not adversely affected by immunocastration. Likewise, immunocastration with Bopriva proved to be effective to stop scrotal development and consequently reduces the sexual and aggressive behavior of young rams. The dose of 0.5 mL seems to be adequate due showed better performance for most variables compared to other treatments