Search

Your search keyword '"Carmen Guerrero-Galán"' showing total 16 results
Start Over Author "Carmen Guerrero-Galán"
16 results on '"Carmen Guerrero-Galán"'

1. Fungal Shaker-like channels beyond cellular K+ homeostasis: A role in ectomycorrhizal symbiosis between Hebeloma cylindrosporum and Pinus pinaster.

Author

Kevin Garcia, Carmen Guerrero-Galán, Hannah E R Frank, Muhammad Zulqurnain Haider, Amandine Delteil, Geneviève Conéjéro, Raphaël Lambilliotte, Cécile Fizames, Hervé Sentenac, and Sabine D Zimmermann

Subjects
Medicine, Science
Abstract

Potassium (K+) acquisition, translocation and cellular homeostasis are mediated by various membrane transport systems in all organisms. We identified and described an ion channel in the ectomycorrhizal fungus Hebeloma cylindrosporum (HcSKC) that harbors features of animal voltage-dependent Shaker-like K+ channels, and investigated its role in both free-living hyphae and symbiotic conditions. RNAi lines affected in the expression of HcSKC were produced and used for in vitro mycorrhizal assays with the maritime pine as host plant, under standard or low K+ conditions. The adaptation of H. cylindrosporum to the downregulation of HcSKC was analyzed by qRT-PCR analyses for other K+-related transport proteins: the transporters HcTrk1, HcTrk2, and HcHAK, and the ion channels HcTOK1, HcTOK2.1, and HcTOK2.2. Downregulated HcSKC transformants displayed greater K+ contents at standard K+ only. In such conditions, plants inoculated with these transgenic lines were impaired in K+ nutrition. Taken together, these results support the hypothesis that the reduced expression of HcSKC modifies the pool of fungal K+ available for the plant and/or affects its symbiotic transfer to the roots. Our study reveals that the maintenance of K+ transport in H. cylindrosporum, through the regulation of HcSKC expression, is required for the K+ nutrition of the host plant.

Published
2020
Full Text
View/download PDF

2. The calcium sensor CBL7 is required for Serendipita indica ‐induced growth stimulation in Arabidopsis thaliana , controlling defense against the endophyte and K + homoeostasis in the symbiosis

Author

Marta‐Marina Pérez‐Alonso, Carmen Guerrero‐Galán, Adrián González Ortega‐Villaizán, Paloma Ortiz‐García, Sandra S. Scholz, Patricio Ramos, Hitoshi Sakakibara, Takatoshi Kiba, Jutta Ludwig‐Müller, Anne Krapp, Ralf Oelmüller, Jesús Vicente‐Carbajosa, and Stephan Pollmann

Subjects
Arabidopsis Proteins, Physiology, Basidiomycota, Calcineurin, Arabidopsis, Plant Science, Plants, Plant Roots, Gene Expression Regulation, Plant, Endophytes, Potassium, Homeostasis, Calcium, Symbiosis, Protein Kinases
Abstract

Calcium is an important second messenger in plants. The activation of Ca

Published
2022
Full Text
View/download PDF

3. Secrets of the fungus-specific potassium channel TOK family

Author

Gabriella Houdinet, Carmen Guerrero-Galán, Benjamin D. Rose, Kevin Garcia, and Sabine D. Zimmermann

Subjects
Microbiology (medical), Infectious Diseases, Virology, Microbiology
Abstract

Several families of potassium (K

Published
2022

4. The calcium sensor CBL7 is required for Serendipita indica -induced growth stimulation in Arabidopsis thaliana, controlling defense against the endophyte and K + homeostasis in the symbiosis

Author

Marta-Marina Pérez-Alonso, Carmen Guerrero-Galán, Adrián González Ortega-Villaizán, Paloma Ortiz-García, Sandra Scholz, Hitoshi Sakakibara, Takatoshi Kiba, Jutta Ludwig-Müller, Anne Krapp, Ralf Oelmüller, Jesús Vicente-Carbajosa, Stephan Pollmann, and Patricio Ramos

Abstract

Calcium (Ca ) is an important second messenger in plants. The activation of Ca signaling cascades is critical in the activation of adaptive processes in response to perceived environmental stimuli, including biotic stresses. The colonization of roots by the plant growth promoting endophyte Serendipita indica involves the increase of cytosolic Ca levels in Arabidopsis thaliana. In this study, we investigated transcriptional changes in Arabidopsis roots during symbiosis with S. indica. RNA-seq profiling disclosed the significant induction of CALCINEURIN B-LIKE 7 ( CBL7) during early- and later phases of the interaction. Consistent with the transcriptomics analysis, reverse genetic evidence and yeast two-hybrid studies highlighted the functional relevance of CBL7 and tested the involvement of a CBL7-CBL-INTERACTING PROTEIN KINASE 13 (CIPK13) signaling pathway in the establishment of the mutualistic relationship that promotes plant growth. The loss-of-function of CBL7 abolished the growth promoting effect of S. indica and affected the colonization of the root by the fungus. The subsequent transcriptomics analysis of cbl7 revealed the involvement of this Ca sensor in activating plant defense responses. Furthermore, we report on the contribution of CBL7 to potassium transport in Arabidopsis. Triggered by the differential expression of a small number of K channels/transporter genes, we analyzed K contents in wild-type and cbl7 plants and observed a significant accumulation of K in root of cbl7 plants, while shoot tissues demonstrated K depletion. Taken together, our work associates CBL7 with an important role in the mutual interaction between Arabidopsis and S. indica and links the CBL7 Ca receptor protein to K transport.

Published
2022
Full Text
View/download PDF

5. Datamining and functional environmental genomics reassess the phylogenetics and functional diversity of fungal monosaccharide transporters

Author

Roland Marmeisse, Danis Abrouk, Delphine Melayah, Laurent Vallon, Sabine Zimmermann, Florian Barbi, Marc Lemaire, Patricia Luis, Carmen Guerrero-Galán, Laurence Fraissinet-Tachet, Jeanne Doré, Laboratoire d'Ecologie Microbienne - UMR 5557 (LEM), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Ecole Nationale Vétérinaire de Lyon (ENVL)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Biochimie et Physiologie Moléculaire des Plantes (BPMP), Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Microbiologie, adaptation et pathogénie (MAP), Université de Lyon-Université de Lyon-Institut National des Sciences Appliquées de Lyon (INSA Lyon), Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Centre National de la Recherche Scientifique (CNRS), Institut de Systématique, Evolution, Biodiversité (ISYEB ), Muséum national d'Histoire naturelle (MNHN)-École pratique des hautes études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Université des Antilles (UA), and CNRS-INSU ECCO Microbien programINRA metaprogramme M2E (project Metascreen)

Subjects
Protein family, Rhamnose, [SDV]Life Sciences [q-bio], Mannose, Xylose, Applied Microbiology and Biotechnology, 03 medical and health sciences, chemistry.chemical_compound, [SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN], Phylogeny, ComputingMilieux_MISCELLANEOUS, [SDV.MP.MYC]Life Sciences [q-bio]/Microbiology and Parasitology/Mycology, Metatranscriptomics, 030304 developmental biology, 2. Zero hunger, 0303 health sciences, Fungal protein, environmental genomics, Ascomycota, biology, 030306 microbiology, Monosaccharides, metatranscriptomics 22 23, sugar transporters, Fungi, Membrane Transport Proteins, Biological Transport, General Medicine, biology.organism_classification, Yeast, Glucose, Biochemistry, chemistry, Galactose, Metagenomics, Biotechnology
Abstract

International audience; Sugar transporters are essential components of carbon metabolism and have been extensively studied to control sugar uptake by yeasts and filamentous fungi used in fermentation processes. Based on published information on characterized fungal sugar porters, we show that this protein family encompasses phylogenetically distinct clades. While several clades encompass transporters that seemingly specialized on specific "sugar-related" molecules (e.g., myo-inositol, charged sugar analogs), others include mostly either mono- or di/oligosaccharide low-specificity transporters. To address the issue of substrate specificity of sugar transporters, that protein primary sequences do not fully reveal, we screened "multi-species" soil eukaryotic cDNA libraries for mannose transporters, a sugar that had never been used to select transporters. We obtained 19 environmental transporters, mostly from Basidiomycota and Ascomycota. Among them, one belonged to the unusual "Fucose H+ Symporter" family, which is only known in Fungi for a rhamnose transporter in Aspergillus niger. Functional analysis of the 19 transporters by expression in yeast and for two of them in Xenopus laevis oocytes for electrophysiological measurements indicated that most of them showed a preference for D-mannose over other tested D-C6 (glucose, fructose, galactose) or D-C5 (xylose) sugars. For the several glucose and fructose-negative transporters, growth of the corresponding recombinant yeast strains was prevented on mannose in the presence of one of these sugars that may act by competition for the binding site. Our results highlight the potential of environmental genomics to figure out the functional diversity of key fungal protein families and that can be explored in a context of biotechnology. KEY POINTS: • Most fungal sugar transporters accept several sugars as substrates. • Transporters, belonging to 2 protein families, were isolated from soil cDNA libraries. • Environmental transporters featured novel substrate specificities.

Published
2021
Full Text
View/download PDF

6. Fungal Shaker-like channels beyond cellular K+ homeostasis: A role in ectomycorrhizal symbiosis between Hebeloma cylindrosporum and Pinus pinaster

Author

Sabine Zimmermann, Kevin Garcia, Carmen Guerrero-Galán, Hervé Sentenac, Cécile Fizames, Geneviève Conejero, Muhammad Zulqurnain Haider, Raphaël Lambilliotte, Hannah E. R. Frank, Amandine Delteil, Department of Crop and Soil Sciences, Biochimie et Physiologie Moléculaire des Plantes (BPMP), Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Plateforme d'histocytologie et d'imagerie cellulaire végétale (PHIV), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Amélioration génétique et adaptation des plantes méditerranéennes et tropicales (UMR AGAP), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), North Carolina State University [Raleigh] (NC State), University of North Carolina System (UNC), Government College University, and French National Research Agency (ANR)2010 BLAN 1604 03

Subjects
0106 biological sciences, 0301 basic medicine, Potassium Channels, Fungal Structure, Physiology, [SDV]Life Sciences [q-bio], Cellular homeostasis, 01 natural sciences, Biochemistry, Ion Channels, Gene Expression Regulation, Fungal, Mycorrhizae, Hebeloma, Homeostasis, Multidisciplinary, Chemistry, Physics, Fungal genetics, Eukaryota, Genomics, Plants, Transport protein, Cell biology, Electrophysiology, Physical Sciences, Medicine, Research Article, Hypha, Science, Biophysics, Neurophysiology, Mycology, Fungal Proteins, 03 medical and health sciences, Symbiosis, Genetics, [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, Fungal Genetics, Ion channel, Fungal Genomics, Organisms, Fungi, Biology and Life Sciences, Proteins, Membrane transport, Pinus, Species Interactions, 030104 developmental biology, Potassium, Shaker Superfamily of Potassium Channels, Physiological Processes, 010606 plant biology & botany, Neuroscience
Abstract

Potassium (K+) acquisition, translocation and cellular homeostasis are mediated by various membrane transport systems in all organisms. We identified and described an ion channel in the ectomycorrhizal fungus Hebeloma cylindrosporum (HcSKC) that harbors features of animal voltage-dependent Shaker-like K+ channels, and investigated its role in both free-living hyphae and symbiotic conditions. RNAi lines affected in the expression of HcSKC were produced and used for in vitro mycorrhizal assays with the maritime pine as host plant, under standard or low K+ conditions. The adaptation of H. cylindrosporum to the downregulation of HcSKC was analyzed by qRT-PCR analyses for other K+-related transport proteins: the transporters HcTrk1, HcTrk2, and HcHAK, and the ion channels HcTOK1, HcTOK2.1, and HcTOK2.2. Downregulated HcSKC transformants displayed greater K+ contents at standard K+ only. In such conditions, plants inoculated with these transgenic lines were impaired in K+ nutrition. Taken together, these results support the hypothesis that the reduced expression of HcSKC modifies the pool of fungal K+ available for the plant and/or affects its symbiotic transfer to the roots. Our study reveals that the maintenance of K+ transport in H. cylindrosporum, through the regulation of HcSKC expression, is required for the K+ nutrition of the host plant.

Published
2020
Full Text
View/download PDF

7. Plant potassium nutrition in ectomycorrhizal symbiosis: properties and roles of the three fungal TOK potassium channels in Hebeloma cylindrosporum

Author

Geneviève Conejero, Gabriella Houdinet, Isabelle Gaillard, Hervé Sentenac, Carmen Guerrero-Galán, Sabine Zimmermann, Kevin Garcia, Amandine Delteil, Biochimie et Physiologie Moléculaire des Plantes (BPMP), Université de Montpellier (UM)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro)-Institut National de la Recherche Agronomique (INRA)-Centre National de la Recherche Scientifique (CNRS), Laboratoire de biochimie et physiologie des plantes, Institut National de la Recherche Agronomique (INRA), Biology and Microbiology Department, South Dakota State University (SDSTATE), Plateforme Histocytologie et Imagerie Cellulaire Végétale, Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad), Zimmermann, Sabine, Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Institut National de la Recherche Agronomique (INRA)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), and Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)

Subjects
0106 biological sciences, 0301 basic medicine, Potassium Channels, Saccharomyces cerevisiae, Fungus, maritime pine, absorption du potassium, champignon mycorhizien, 01 natural sciences, Microbiology, nutrition minérale, Ectosymbiosis, 03 medical and health sciences, Symbiosis, Mycorrhizae, Botany, Hebeloma, ion potassium, [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, Axenic, Ecology, Evolution, Behavior and Systematics, Minerals, Vegetal Biology, pinus pinaster, shaker potassium channel, biology, fungi, Biological Transport, 15. Life on land, Pinus, biology.organism_classification, Yeast, Complementation, hebeloma cylindrosporum, 030104 developmental biology, Seedlings, Potassium, Pinus pinaster, canaux potassiques shaker, Plant nutrition, Biologie végétale, 010606 plant biology & botany
Abstract

Ectomycorrhizal fungi play an essential role in the ecology of boreal and temperate forests through the improvement of tree mineral nutrition. Potassium (K+ ) is an essential nutrient for plants and is needed in high amounts. We recently demonstrated that the ectomycorrhizal fungus Hebeloma cylindrosporum improves the K+ nutrition of Pinus pinaster under shortage conditions. Part of the transport systems involved in K+ uptake by the fungus has been deciphered, while the molecular players responsible for the transfer of this cation towards the plant remain totally unknown. Analysis of the genome of H. cylindrosporum revealed the presence of three putative tandem-pore outward-rectifying K+ (TOK) channels that could contribute to this transfer. Here, we report the functional characterization of these three channels through two-electrode voltage-clamp experiments in oocytes and yeast complementation assays. The expression pattern and physiological role of these channels were analysed in symbiotic interaction with P. pinaster. Pine seedlings colonized by fungal transformants overexpressing two of them displayed a larger accumulation of K+ in shoots. This study revealed that TOK channels have distinctive properties and functions in axenic and symbiotic conditions and suggested that HcTOK2.2 is implicated in the symbiotic transfer of K+ from the fungus towards the plant.

Published
2018
Full Text
View/download PDF

8. Harnessing symbiotic plant-fungus interactions to unleash hidden forces from extreme plant ecosystems

Author

Anne Krapp, Jutta Ludwig-Müller, Stephan Pollmann, Sandra S. Scholz, Carmen Guerrero-Galán, Marta-Marina Pérez-Alonso, Jesús Vicente-Carbajosa, Ralf Oelmüller, Hitoshi Sakakibara, Takatoshi Kiba, Centro de Biotecnología y Genómica de Plantas, Universidad Politécnica de Madrid (UPM)-Instituto Nacional de Investigación y Tecnología Agraria y Alimentación (INIA), Campus de Montegancedo-Campus de Montegancedo, Matthias Schleiden Institute of Genetics, Bioinformatics and Molecular Botany, Department of Plant Physiology, Friedrich-Schiller-Universität = Friedrich Schiller University Jena [Jena, Germany]-Friedrich-Schiller-Universität = Friedrich Schiller University Jena [Jena, Germany], RIKEN Center for Sustainable Resource Science [Yokohama] (RIKEN CSRS), RIKEN - Institute of Physical and Chemical Research [Japon] (RIKEN), Department of Applied Biosciences, Graduate School of Bioagricultural Sciences, Nagoya University-Nagoya University, Institute of Botany, Technische Universität Dresden = Dresden University of Technology (TU Dresden), Institut Jean-Pierre Bourgin (IJPB), AgroParisTech-Université Paris-Saclay-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Departamento de Biotecnología-Biología Vegetal, Escuela Técnica Superior de Ingeniería Agronómica, Alimentaria y de Biosistemas, Universidad Politécnica de Madrid (UPM)-Universidad Politécnica de Madrid (UPM), and Ministry of Economy and Competitiveness (MINECO), Spain PCIN-2016037Federal Ministry of Education & Research (BMBF)01DR17007A01DR17007BJapan Science & Technology Agency (JST)JPMJSC16C3Centre National de la Recherche Scientifique (CNRS)EIG_JC1JAPAN-045Severo Ochoa Program for Centers of Excellence in R&D from the Agencia Estatal de Investigacion, Spain SEV-2016-0672

Subjects
0106 biological sciences, Mediterranean climate, Serendipita indica, plant performance, Physiology, Natural resource economics, Climate Change, Climate change, Growing season, Plant Science, 01 natural sciences, crosstalk, 03 medical and health sciences, [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, Ecosystem, Agricultural productivity, Review Papers, 030304 developmental biology, 2. Zero hunger, 0303 health sciences, endosymbiosis, business.industry, AcademicSubjects/SCI01210, Basidiomycota, Global warming, fungi, Fungi, food and beverages, 15. Life on land, Abiotic stress, Europe, Geography, 13. Climate action, Agriculture, Greenhouse gas, business, 010606 plant biology & botany
Abstract

Serendipita indica can colonize a broad range of hosts, conferring improved stress tolerance and performance under adverse conditions. The underlying molecular mechanisms have the potential to improve agricultural practices in a climate change scenario., Global climate change is arguably one of the biggest threats of modern times and has already led to a wide range of impacts on the environment, economy, and society. Owing to past emissions and climate system inertia, global climate change is predicted to continue for decades even if anthropogenic greenhouse gas emissions were to stop immediately. In many regions, such as central Europe and the Mediterranean region, the temperature is likely to rise by 2–5 °C and annual precipitation is predicted to decrease. Expected heat and drought periods followed by floods, and unpredictable growing seasons, are predicted to have detrimental effects on agricultural production systems, causing immense economic losses and food supply problems. To mitigate the risks of climate change, agricultural innovations counteracting these effects need to be embraced and accelerated. To achieve maximum improvement, the required agricultural innovations should not focus only on crops but rather pursue a holistic approach including the entire ecosystem. Over millions of years, plants have evolved in close association with other organisms, particularly soil microbes that have shaped their evolution and contemporary ecology. Many studies have already highlighted beneficial interactions among plants and the communities of microorganisms with which they coexist. Questions arising from these discoveries are whether it will be possible to decipher a common molecular pattern and the underlying biochemical framework of interspecies communication, and whether such knowledge can be used to improve agricultural performance under environmental stress conditions. In this review, we summarize the current knowledge of plant interactions with fungal endosymbionts found in extreme ecosystems. Special attention will be paid to the interaction of plants with the symbiotic root-colonizing endophytic fungus Serendipita indica, which has been developed as a model system for beneficial plant–fungus interactions.

Published
2019
Full Text
View/download PDF

9. The ectomycorrhizal contribution to tree nutrition

Author

Sabine Zimmermann, Janice L. Eibensteiner, Kevin Garcia, Heike Bücking, Carmen Guerrero-Galán, Adeline Becquer, Gabriella Houdinet, Ecologie fonctionnelle et biogéochimie des sols et des agro-écosystèmes (UMR Eco&Sols), Institut National de la Recherche Agronomique (INRA)-Institut de Recherche pour le Développement (IRD)-Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Commonwealth Scientific and Industrial Research Organisation (CSIRO), Biochimie et Physiologie Moléculaire des Plantes (BPMP), Université de Montpellier (UM)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro)-Institut National de la Recherche Agronomique (INRA)-Centre National de la Recherche Scientifique (CNRS), Biology and Microbiology Department, South Dakota State University (SDSTATE), Department of Crop and Soil Sciences, North Carolina State University [Raleigh] (NC State), University of North Carolina System (UNC), Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut de Recherche pour le Développement (IRD)-Institut National de la Recherche Agronomique (INRA), Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Institut National de la Recherche Agronomique (INRA)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), and Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)

Subjects
0106 biological sciences, composé soufre, symbiose ectomycorhizienne, 01 natural sciences, Ectosymbiosis, 03 medical and health sciences, Nutrient, sulphured compound, Symbiosis, absorption azotée, absorption de l'eau, [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, Ecosystem, système de transport cellulaire, phosphorus, 030304 developmental biology, Mutualism (biology), 0303 health sciences, arbre, Ascomycota, biology, Ecology, potassium, fungi, zinc, food and beverages, 15. Life on land, biology.organism_classification, phosphore, Temperate rainforest, 010606 plant biology & botany, Woody plant
Abstract

Trees can be associated with dozens of fungi helping them to acquire resources from forest soils. The most widespread mutualistic association in boreal and temperate forests is the ectomycorrhizal symbiosis. This symbiosis involves mushroom-forming fungi of basidiomycota, ascomycota, and some zygomycota clades and the roots of woody plant species, including oaks, poplars or pines. Although the impact of this association on ecosystem production and tree nutrition is investigated for about a century, our understanding on the molecular mechanisms that control water and nutrient fluxes between plant and fungal partners is still limited. Here, we review the recent knowledge on the ectomycorrhizal contribution to tree nutrition. We specifically highlight the molecular mechanisms driving the acquisition, translocation and release of water and nutrients in ectomycorrhizal systems. We particularly focus on the transport of macronutrients, including nitrogen, phosphorus, potassium, sulphur and calcium, micronutrients, and water by the symbiotic partner. We also provide background on the evolution, diversity, and importance of this symbiosis, identify knowledge gaps, and propose future research directions.

Published
2019
Full Text
View/download PDF

10. Ectomycorrhizal symbiosis helps plants to challenge salt stress conditions

Author

Carmen Guerrero-Galán, Monica Calvo-Polanco, Sabine Zimmermann, Biochimie et Physiologie Moléculaire des Plantes (BPMP), Université de Montpellier (UM)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro)-Institut National de la Recherche Agronomique (INRA)-Centre National de la Recherche Scientifique (CNRS), Equipe Electrophysiologie de la nutrition minérale et des symbioses racinaires (ELSA), Université de Montpellier (UM)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro)-Institut National de la Recherche Agronomique (INRA)-Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro)-Institut National de la Recherche Agronomique (INRA)-Centre National de la Recherche Scientifique (CNRS), Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Institut National de la Recherche Agronomique (INRA)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), and Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)

Subjects
0106 biological sciences, Soil salinity, Osmotic shock, [SDV]Life Sciences [q-bio], Soil biology, Plant Science, Biology, 010603 evolutionary biology, 01 natural sciences, Plant Roots, Salt Stress, Trees, Ectosymbiosis, Membrane transporters, Symbiosis, Mycorrhizae, Botany, Genetics, Salt tolerance, [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, Molecular Biology, Ecology, Evolution, Behavior and Systematics, 2. Zero hunger, Water transport, Ectomycorrhizal (ECM) symbiosis, Sodium, Fungi, Water, General Medicine, [SDV.BV.BOT]Life Sciences [q-bio]/Vegetal Biology/Botanics, 15. Life on land, Nutrient and water transport, Salinity, [SDV.EE.IEO]Life Sciences [q-bio]/Ecology, environment/Symbiosis, 010606 plant biology & botany, Woody plant
Abstract

International audience; Soil salinity is an environmental condition that is currently increasing worldwide. Plant growth under salinity induces osmotic stress and ion toxicity impairing root water and nutrient absorption, but the association with beneficial soil microorganisms has been linked to an improved adaptation to this constraint. The ectomycorrhizal (ECM) symbiosis has been proposed as a key factor for a better tolerance of woody species to salt stress, thanks to the reduction of sodium (Na+) uptake towards photosynthetic organs. Although no precise mechanisms for this enhanced plant salt tolerance have been described yet, in this review, we summarize the knowledge accumulated so far on the role of ECM symbiosis. Moreover, we propose several strategies by which ECM fungi might help plants, including restriction of Na+ entrance into plant tissues and improvement of mineral nutrition and water balances. This positive effect of ECM fungi has been proven in field assays and the results obtained point to a promising application in forestry cultures and reforestation.

Published
2018
Full Text
View/download PDF

11. Unravelling nutrient exchange in ectomycorrhizal symbiosis contributing to plant potassium nutrition

Author

Gabriella Houdinet, Carmen Guerrero-Galán, Kevin Garcia, Amandine Delteil, claire corratgé-faillie, Geneviève Conejero, Isabelle Gaillard, Bruno Touraine, Herve Sentenac, Sabine Zimmermann, Biochimie et Physiologie Moléculaire des Plantes (BPMP), Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Institut National de la Recherche Agronomique (INRA)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), and Université de Montpellier (UM)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro)-Institut National de la Recherche Agronomique (INRA)-Centre National de la Recherche Scientifique (CNRS)

Subjects
symbiose ectomycorhizienne, [SDV]Life Sciences [q-bio], fungi, education, food and beverages, [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, nutrition potassique, social sciences, health care economics and organizations
Abstract

Unravelling nutrient exchange in ectomycorrhizal symbiosis contributing to plant potassium nutrition. 12th Congress of the International Plant Molecular Biology

Published
2018

12. Unravelling nutrient exchange in ectomycorrhizal symbiosis contributing to plant potassium nutrition

Author

Maria del Carmen Guerrero-Galán, Gabriella Houdinet, Amandine Delteil, Kevin Garcia, Sabine D. Zimmermann, Biochimie et Physiologie Moléculaire des Plantes (BPMP), Université de Montpellier (UM)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro)-Institut National de la Recherche Agronomique (INRA)-Centre National de la Recherche Scientifique (CNRS), Equipe Electrophysiologie de la nutrition minérale et des symbioses racinaires (ELSA), Université de Montpellier (UM)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro)-Institut National de la Recherche Agronomique (INRA)-Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro)-Institut National de la Recherche Agronomique (INRA)-Centre National de la Recherche Scientifique (CNRS), Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Institut National de la Recherche Agronomique (INRA)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), and Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)

Subjects
pinus, interaction sol racine, [SDV]Life Sciences [q-bio], symbiose ectomycorhizienne, [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, nutrition potassique, hebeloma, ComputingMilieux_MISCELLANEOUS
Abstract

A major role of mutualistic interactions between plant roots and soil fungi is the improvement of plant nutrition by an equitable exchange of nutrients leading also to tolerance of environmental stress. Ectomycorrhizal symbiosis established between woody plants and soil fungi, widespread in forest ecosystems, is crucial for the plant partner to efficiently take up poorly available nutrients. Physiological studies as well as recent genome sequencing projects (Kohler et al. (2015) Nature Genet 47: 410-415) and transcriptome analyses (Doré et al. (2015) New Phytol 208: 1169-1187; Doré et al. (2017) Environ Microbiol 19:1338-1354) have allowed progress towards the identification and characterization of the symbiotic transportome (Garcia et al. (2016) Trends in Plant Science 21: 937-950). One of the major nutrients is potassium (K+ ), the most abundant cation in plant cells. We have shown improvement of potassium nutrition (Garcia et al. (2014) New Phytol 201: 951-960) under K+ shortage conditions by ectomycorrhizal symbiosis using two model species from European forests, Pinus pinaster and Hebeloma cylindrosporum. Questions are raised to identify the transport systems involved in the uptake of nutrients from the soil and in their transfer towards the plant at the symbiotic fungus-plant interface, called Hartig net. In the case of potassium (Garcia and Zimmermann (2014) Front Plant Sci 5: 337), we have identified two types of K+ transporters, Trk and HAK, as candidates to perform K+ uptake from the soil by the fungal extraradical hyphae, and two types of K+ channels, Shaker-like and TOK, that may release K+ by the hyphae of the Hartig net into the plant apoplasm. We have studied the three TOK (Two-pore Outward K+) channels identified in the genome of H. cylindrosporum, a channel family specific for fungi initially described in yeast (Ketchum et al. (1995) Nature 376: 690-695). These three TOK channels from the ectomycorrhizal fungus H. cylindrosporum belonging to two different subfamilies have been functionally characterized and localized (Carmen Guerrero-Galán et al. (2018) Env Microbiol , in press). Finally, we have analyzed whether these K+ channels might play specific roles within the fungus and within the symbiosis.

Published
2018

13. HcTOK1 participates in the maintenance of K+ homeostasis in the ectomycorrhizal fungus Hebeloma cylindrosporum, which is essential for the symbiotic K+ nutrition of Pinus pinaster

Author

Kevin Garcia, Sabine Zimmermann, Carmen Guerrero-Galán, Gabriella Houdinet, Biochimie et Physiologie Moléculaire des Plantes (BPMP), Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Institut National de la Recherche Agronomique (INRA)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Université de Montpellier (UM)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro)-Institut National de la Recherche Agronomique (INRA)-Centre National de la Recherche Scientifique (CNRS), Equipe Electrophysiologie de la nutrition minérale et des symbioses racinaires (ELSA), Université de Montpellier (UM)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro)-Institut National de la Recherche Agronomique (INRA)-Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro)-Institut National de la Recherche Agronomique (INRA)-Centre National de la Recherche Scientifique (CNRS), Biochimie et Physiologie Moléculaire des Plantes ( BPMP ), Centre international d'études supérieures en sciences agronomiques ( Montpellier SupAgro ) -Institut national de la recherche agronomique [Montpellier] ( INRA Montpellier ) -Université de Montpellier ( UM ) -Centre National de la Recherche Scientifique ( CNRS ) -Institut national d’études supérieures agronomiques de Montpellier ( Montpellier SupAgro ), Equipe Electrophysiologie de la nutrition minérale et des symbioses racinaires ( ELSA ), and Centre international d'études supérieures en sciences agronomiques ( Montpellier SupAgro ) -Institut national de la recherche agronomique [Montpellier] ( INRA Montpellier ) -Université de Montpellier ( UM ) -Centre National de la Recherche Scientifique ( CNRS ) -Institut national d’études supérieures agronomiques de Montpellier ( Montpellier SupAgro ) -Centre international d'études supérieures en sciences agronomiques ( Montpellier SupAgro ) -Institut national de la recherche agronomique [Montpellier] ( INRA Montpellier ) -Université de Montpellier ( UM ) -Centre National de la Recherche Scientifique ( CNRS ) -Institut national d’études supérieures agronomiques de Montpellier ( Montpellier SupAgro )

Subjects
0106 biological sciences, 0301 basic medicine, Hartig net, [ SDV.BV ] Life Sciences [q-bio]/Vegetal Biology, biology, fungi, Plant Science, Fungus, 15. Life on land, biology.organism_classification, 01 natural sciences, Yeast, Ectosymbiosis, 03 medical and health sciences, 030104 developmental biology, Ion homeostasis, Symbiosis, Botany, Pinus pinaster, [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, Plant nutrition, 010606 plant biology & botany
Abstract

Most land plants rely on root symbioses to complement or improve their mineral nutrition. Recent researches have put forward that mycorrhizal fungi efficiently absorb and transfer potassium (K+) from the soil to host plant roots, but the molecular mechanisms involved are not completely elucidated yet. We have recently revealed that K+ is likely released from the fungal Hartig net to the plant by TOK channels in the ectomycorrhizal model Hebeloma cylindrosporum - Pinus pinaster. H. cylindrosporum harbours three TOK members. Herein, we report that one of them, HcTOK1, has similar features than the yeast ScTOK1. Moreover, we propose a role for this channel in the transport of K+ from the medium to ectomycorrhizal roots under K+ starvation.

Published
2018
Full Text
View/download PDF

14. Fungal molecular players involved in plant potassium nutrition via ectomycorrhizal associations

Author

Carmen Guerrero-Galán, Amandine Delteil, Kevin Garcia, Herve Sentenac, Sabine D. Zimmermann, Biochimie et Physiologie Moléculaire des Plantes (BPMP), Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Institut National de la Recherche Agronomique (INRA)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), and Université de Montpellier (UM)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro)-Institut National de la Recherche Agronomique (INRA)-Centre National de la Recherche Scientifique (CNRS)

Subjects
shaker potassium channel, canal à potassium, education, fungi, ion potassium, [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, canaux potassiques shaker, human activities
Abstract

Fungal molecular players involved in plant potassium nutrition via ectomycorrhizal associations. 2nd International Molecular Mycorrhiza Meeting IMMM 2015

Published
2015

15. Unravelling nutrient exchange in ectomycorrhizal symbiosis

Author

Carmen Guerrero-Galán, Gabriella Houdinet, Amandine Delteil, Kevin Garcia, Geneviève Conejero, Herve Sentenac, Sabine D. Zimmermann, Biochimie et Physiologie Moléculaire des Plantes (BPMP), Université de Montpellier (UM)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro)-Institut National de la Recherche Agronomique (INRA)-Centre National de la Recherche Scientifique (CNRS), Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Institut National de la Recherche Agronomique (INRA)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), and Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)

Subjects
[SDV]Life Sciences [q-bio], symbiose ectomycorhizienne, [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, champignon ectomycorhizien, absorption du potassium
Abstract

Unravelling nutrient exchange in ectomycorrhizal symbiosis. 3rd international Molecular Mycorrhiza Meeting iMMM

16. Novel functions of fungal TOK channels: putative roles in symbiotic potassium transfer

Author

Carmen Guerrero-Galán, Amandine Delteil, Kevin Garcia, Gabriella Houdinet, Geneviève Conejero, Herve Sentenac, Sabine D. Zimmermann, Biochimie et Physiologie Moléculaire des Plantes (BPMP), Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Institut National de la Recherche Agronomique (INRA)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), and Université de Montpellier (UM)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro)-Institut National de la Recherche Agronomique (INRA)-Centre National de la Recherche Scientifique (CNRS)

Subjects
dépendance mycorhizienne, canal à potassium, [SDV]Life Sciences [q-bio], education, fungi, méthode de transfert, [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, health care economics and organizations
Abstract

Novel functions of fungal TOK channels: putative roles in symbiotic potassium transfer. 9th International Conference on Mycorrhiza, ICOM9

Catalog

Books, media, physical & digital resources
See catalog results