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Este texto tem por finalidade apresentar as experiências do evento de extensão denominado “Festival Paralímpico do Vale do Ivaí” de Ivaiporã, PR, realizado anualmente pelo Curso de Educação Física do Departamento de Ciências do Movimento Humano da Universidade Estadual de Maringá, Campus Regional do Vale do Ivaí (DMO/UEM/CRV). O objetivo do evento é promover a inclusão social de pessoas com deficiências matriculadas na rede regular de ensino e escolas de educação especial, por meio da vivência prática para-desportiva do atletismo, atribuindo visibilidade as potencialidades desta população e, também, proporcionar aos acadêmicos o conhecimento teórico-prático da gestão e organização de um evento esportivo. O texto evidencia como é realizada a organização do Festival e apresenta um resgate histórico de sua trajetória. Destaca-se ao longo das edições, a expansão do evento entre os municípios da região centro-norte do estado, o aumento no número de escolas e pessoas com deficiência participantes e o maior envolvimento acadêmico e da comunidade em geral. As provas realizadas no evento caracterizam-se como de pista: sprint, fundo, meio fundo e revezamento e, provas de campo, as quais apresentam pouca variação desde a primeira edição. Conclui-se que a organização do evento é sempre marcada por inúmeros desafios que vão desde o planejamento, a logística, a infraestrutura e, especialmente, aos recursos disponíveis para atender todas as demandas e necessidades do público alvo. Entretanto, os legados do evento atingem tanto a esfera social quanto acadêmica e se configura como uma prática bem sucedida para a promoção da atividade física e esportiva para as pessoas com deficiência, para a integração e inclusão social e para a formação acadêmica/profissional qualificada.

O traslado de corpos é um processo de transferência de um cadáver ou restos mortais de um local para o outro, que pode ser de um país para outro. Os países abordados por esse estudo são Brasil e Portugal. Alguns requisitos são obrigatórios para conclusão do processo e acompanhados pelo Consulado dos países envolvidos. Dentre os documentos estão: certidão de óbito e certidão de embalsamento, além disso, o transporte internacional deve ser realizado através do modal aéreo, por ser considerado mais seguro e adequado para este tipo de procedimento. Este modal contribui para a preservação do cadáver e permite maior agilidade nos procedimentos para o acompanhamento e a liberação do cadáver por parte dos envolvidos na identificação e reconhecimento deste. Esse serviço é essencial para oferecer suporte a familiares de imigrantes que infelizmente vieram a falecer fora de sua terra natal. Essa pesquisa está baseada em pesquisa bibliográfica sobre temas específicos fundamentados em livros, teses, artigos científicos. A abordagem da pesquisa é qualitativa com a apresentação dos conceitos relevantes para o entendimento, análise e conclusão do tema desta pesquisa., Keywords: Transfer. Corpse. Transport.

This study aimed to use an intelligent formulation design for the development of mini-tablets for the modified release of morphine sulfate. A formulation (F1) was proposed using the Hiperstart® software. Based on the suggested formulation, two other formulations (F2 and F3) were prepared: one for modified and another for immediate drug release. The powders were characterized as bulk and tapped density, Hausner's factor, and compressibility index analyses. Mini-tablets were directly compressed and characterized by hardness, friability, size, and weight variation. The

Gastroretentive drug delivery systems (GRDDS) are modified-release dosage forms designed to prolong their residence time in the upper gastrointestinal tract, where some drugs are preferentially absorbed, and increase the drug bioavailability. This work aimed the development of a novel GRDDS containing 60 mg of sildenafil citrate, and the evaluation of the feasibility of the proposed formulation for use in the treatment of pulmonary arterial hypertension (PAH), for once a day administration, by using in silico pharmacokinetic (PK) modeling and simulations using GastroPlus

Background Leukoreduction is a routine procedure in human transfusion medicine but is uncommon in veterinary. Objectives To evaluate the effect of leukoreduction on the quality of canine whole blood (WB) and blood products during storage. Animals Ten canine blood donors. Methods This is a case series study. An amount of 450 mL of blood was collected from each dog. Five WB and 5 packed red blood cells (pRBC) bags were divided into 2 units each: leukoreduced (LR) and non‐leukoreduced (nLR). RBC count, erythrocytes' mean osmotic fragility (MOF), 2,3‐diphosphoglycerate (2,3‐DPG), adenosine triphosphate (ATP), percentage of hemolysis, potassium (K), lactate, glucose, and cytokines were measured weekly from day of donation (T0) to day 35 (T35); pH, coagulation times, and clotting factors were evaluated at T0 and T35 from WB and in fresh frozen plasma after 1 year of storage. Results Leukoreduction showed positive effects on lactate (T35: LR WB 14.42 mmol/L SD 2.71, nLR WB 22.42 mmol/L SD 1.86, LR pRBC 20.88 mmol/L SD 2.65, nLR pRBC 36.81 mmol/L SD 2.34; P

The heterogeneous morphologic features of canine plasmacytomas (PCTs) can make their differentiation from other round cell tumors challenging. Immunohistochemistry (IHC) for lambda (λ) and kappa (к) immunoglobulin (Ig) light chains is often equivocal because of high background staining. The chromogenic in situ hybridization (CISH) technique for light chains has shown higher sensitivity compared to IHC in human plasma cell tumors. Therefore, we aimed to validate automated CISH for light chains in canine tissues and to evaluate its diagnostic potential in canine PCTs, in conjunction with routinely used IHC markers. CISH for light chains demonstrated a clear signal in plasma cell populations of canine control tissues (lymph nodes, lymphoplasmacytic inflammation) showing a polyclonal pattern with a prevalence of λ-producing cells. CISH detected monotypic light chain expression in 33 of 53 (62%) PCTs, 31 expressing λ and 2 expressing к. CISH was more sensitive than IHC for λ light chain (58% vs. 47%, respectively) and more easily interpretable given the absence of confounding background staining. The absence of CISH staining for both λ and к in a considerable subset of tumors may be the result of lower light chain production by neoplastic cells. Multiple myeloma oncogene 1 (MUM1) was expressed by all but 2 PCTs (96%), which showed λ expression by CISH and IHC. The identification of poorly differentiated canine PCTs requires the assessment of a panel of IHC markers, with the potential support of CISH for Ig light chains.

Dogs are proved to be competent reservoir hosts for several vector-borne pathogens. Their prevalence varies according to the geographical area. Many vector-borne pathogens may be transmitted by blood transfusion. The purpose of this study was to determine the serological and molecular prevalence of some vector-borne pathogens in dog blood donors, living in central Italy. Blood samples of 126 donors (19 breeds) included were tested for a broad serological and DNA-base tests panel. The differences in pathogen prevalence according to age, sex, and breeds were tested (chi-square test, Fisher’s exact test). Overall, 50 animals (39.7%) tested positive at PCR (polymerase chain reaction) and/or serology (IFAT, indirect fluorescent antibody test) for at least one pathogen. Three dogs were positive at both serology and PCR. A tendency of hemoplasmas to be more prevalent in older dogs (41.2%) compared to the younger ones (25.7%) was noted. We highlight the difficulties of selecting healthy blood donor dogs in an endemic area for vector-borne infections. It is important to choose the serological and biomolecular investigations panel that is most suited to the donor’s environment. Close collaboration between clinician and parasitologists is important in the interpretation of IFAT and PCR results. Finally, we underline the important role of blood donors as an epidemiological tool for active surveillance against canine vector-borne diseases.

An 8-month-old female Main Coon with a history of recurrent behavioral changes and anorexia was presented with sternal recumbency and depression. Within 5 days, the cat progressively worsened with symptoms of stupor and coma and was euthanized. At post-mortem examination, a solid, grayish infratentorial mass located in the midline rostrally to the cerebellum, was observed. Histologically, highly cellular clusters of small-to-medium undifferentiated cells were intermingled with paucicellular areas with fibrillary eosinophilic (neuropil-like) appearance. Numerous multilayered (ependymoblastic) true rosettes were present. The mitotic activity was frequent (up to 15 mitoses/HPF), involving both undifferentiated cells and rosettes. By immunohistochemistry (IHC), tumor cells were diffusely positive for vimentin, variably for synaptophysin, S-100, and NSE, and focally for NeuN; they were negative for GFAP and CK AE1/AE3. The histological and IHC aspects were consistent with an Embryonal Tumor with Abundant Neuropil and True Rosettes (ETANTR). Embryonal neoplasms of the central nervous system (CNS) are characterized by primitive undifferentiated cells, able to develop toward neuronal, glial, ependymal, and mesenchymal lines. Although extremely rare, juvenile embryonal tumors should be considered in the differentials of CNS disorders in young cats.

Apesar da alta frequência de intoxicações na clínica médica de pe­quenos animais, acidentes com anfíbios são considerados emergên­cias médicas devido ao elevado potencial tóxico de seu veneno, o qual tende a direcionar o curso clínico do paciente intoxicado para eventos fisiopatológicos, principalmente cardiovasculares e neuro­lógicos. Este estudo tem por finalidade descrever as principais alte­rações clínicas e laboratoriais observadas em um cão intoxicado por veneno de sapo identificado como sendo Rhinella icterica (Anura: Bufonidae), mediante identificação do anfíbio por imagens obtidas pelos tutores. A instituição precoce das estratégias terapêuticas ime­diatas (estabilização clínica) e tardias (terapia intensiva), assim como o estadiamento de lesões em outros órgãos e sistemas pelos exames complementares realizados de maneira simultânea, caracterizaram-se como essenciais para a melhora no prognóstico do animal.

O controle de estímulos exercido por algumas propriedades ou aspectos de um estímulo antecedente contingente ao reforço é definido como controle restrito de estímulos. O objetivo do estudo foi investigar o controle discriminativo por elementos de estímulos visuais compostos em tarefas de discriminação simples. Participaram catorze pré-escolares (seis com autismo e oito com desenvolvimento típico) entre quatro e seis anos de idade. O procedimento envolveu o ensino de discriminação simples entre um par de estímulos visuais compostos por dois elementos. Em seguida, foram realizadas sondas para avaliar o controle discriminativo por cada elemento do estímulo composto (S+ e S-). Todas as crianças responderam aos dois elementos do S+ e não responderam, ou responderam com baixa frequência, aos elementos do S- dos três pares de estímulos treinados e avaliados separadamente. Os resultados demonstraram que os pré-escolares com autismo e desenvolvimento típico responderam sob controle dos elementos dos estímulos S+. O investimento no estudo do controle restrito de estímulos pode contribuir na compreensão desse fenômeno como um processo comportamental básico.

Background The ability of tick-borne agents to survive in stored blood bags is a key factor for their transmissibility by blood transfusion. The aim of this study was to evaluate the survival and potential infectivity of Rickettsia conorii (RC) in artificially contaminated canine whole blood (WB) and in leukoreduced whole blood (LR-WB) during the storage period. Methods RC was cultured on L929 cells. We used a one-week 25-cm2 flask with 70–80% of L929 infected cells to prepare the bacterial inoculum by pelleting cells and suspending the pellet in the donors’ serum. We infected five 100 ml WB units with RC within 2 h from the collection and maintained it at room temperature for 4 h prior to refrigeration. We filtered 50 ml of each WB bag to obtain leukoreduced WB (LR-WB) at day 1 post-infection (dpi). We checked WB and LR-WB bags at 1, 4, 7, 14, 21, 28, 35 dpi for RC presence and viability through real-time PCR (rPCR) for DNA and mRNA, respectively, and by isolation. Identification of isolates was confirmed by indirect immunofluorescence and rPCRs. Results RC survived for the entire storage period in both whole and leukoreduced blood. All bags contained viable bacteria until 7 dpi; RC viability generally decreased over time, particularly in LR-WB bags where the isolation time was longer than in WB. Viable bacteria were still isolated at 35 dpi in 3 WB and 3 LR-WB. Conclusions Leukoreduction reduced but did not eliminate RC in infected units. The survival and infectivity of RC in canine blood during the storage period may represent a threat for recipients.

The article proposes a set of requirements to be used in assessing the potential of the River Basin Management Plans as instruments: (i) of (vertical) articulation between river basin planning and national and state planning of water resources; (ii) of articulation (horizontal) between water resources planning and other sectorial planning; (iii) of integration between water resources planning and regional and municipal strategies for land use planning and environmental sanitation; (iv) of water safety. These requirements were applied to the Alto-Tietê River Basin Management Plan, which demonstrated low potential for horizontal articulation and water safety planning. To overcome these limitations, it is suggested, in the next plan revisions, the use of planning tools that promote the involvement of society in a complementary way to the discussions in the river basin committee, fostering shared decision-making between the different actors and user sectors. Resumen El artículo propone un conjunto de requisitos a ser utilizado en la evaluación de los Planes de Ordenación y Manejo de Cuencas Hidrográficas (POMCA) como instrumentos de: (i) articulación (vertical) entre la planificación de cuencas hidrográficas y la planificación nacional y estatal de los recursos hídricos; (ii) de articulación (horizontal) entre la planificación de los recursos hídricos y otra planificación sectorial; (iii) de integración entre la planificación de los recursos hídricos y las estrategias regionales y municipales para la ordenación del territorio y el saneamiento ambiental y; (iv) de seguridad del agua. Tales requisitos fueron aplicados al POMCA del Alto-Tietê, que demostró bajo potencial para la articulación horizontal y la planificación de la seguridad del agua. Se sugiere que en las próximas revisiones del POMCA, se promuevan la participación pública de manera complementaria a las discusiones dentro del comité de cuenca, fomentando la decisión compartida entre los sectores de usuarios. Resumo O artigo propõe um conjunto de requisitos a ser utilizado na avaliação do potencial dos Planos de Bacia Hidrográfica como instrumentos de: (i) articulação (vertical) entre o planejamento da bacia hidrográfica e os planejamentos nacional e estadual dos recursos hídricos; (ii) de articulação (horizontal) entre planejamento dos recursos hídricos e outros planejamentos setoriais; (iii) de integração entre o planejamento dos recursos hídricos e as estratégias regionais e municipais de ordenamento territorial e saneamento ambiental e; (iv) de segurança hídrica. Tais requisitos foram aplicados ao Plano da Bacia Hidrográfica do Alto Tietê, que mostrou baixo potencial de articulação horizontal e de planejamento da segurança hídrica. Visando à superação de tais limitações, sugere-se que, nas próximas revisões do Plano, sejam utilizadas ferramentas de planejamento que promovam o envolvimento da sociedade, de forma complementar às discussões no âmbito do comitê de bacia, fomentando a decisão compartilhada entre os diversos atores e setores usuários.

In the present study European sea bass (Dicentrarchus labrax) subjected to two different diets (organic vs. conventional) were evaluated in terms of growing performances, oxidative stress, and contaminant markers. Growing performances were evaluated using biometric measures and condition factor (K), whereas insulin-like growth factor (IGF-I and IGF-II) levels were assessed trough Real-Time PCR analysis. For oxidative stress, immunohistochemical staining for 8-hydroxy-2&prime, deoxyguanosine (8-OHdG) and 4-hydroxy-2-nonenal (HNE) was performed, whereas total glutathione (GSH) in blood serum was determined by an enzymatic method adapted. Cytochrome P4501A (CYP1A) and melanomacrophage centers (MMCs) were evaluated as contaminant markers trough immunohistochemical and histochemical approaches, respectively. The growing performances showed a positive trend in both groups but a greater productivity in conventional fed fish compared to the organic ones. A significant higher expression of MMCs was observed in organic vs. conventional diet fed fish. Fillet analysis showed a higher MUFA content and a lower PUFAs n-6 content in organically fed sea bass indicating that diets with a content in fatty acids closer to that of wild fish will definitely affect the fatty acid profile of the fish flesh. On the other hand, the diet composition did not seem to affect neither the oxidative stress parameters (GSH, 8-OHdG, HNE) nor the CYP1A expression.

Additional file 1: Table S1. Detailed results of the tests performed on blood units at different days post-infection (dpi) and on cultures from days 3 to 28. Rickettsia conorii was considered viable (v) or non-viable (nv), when the unit or the culture were positive/negative by RT-rPCR and/or immunofluorescence (IF).

Background Optimal procedure for storage of feline blood is needed. Open‐collection systems have been employed in feline medicine, thus limiting the possibility for storage. Objectives To evaluate indicators of quality of feline blood stored for 35 days at +4°C in a closed‐collection system specifically designed for cats. Animals Eight healthy adult European domestic shorthair cats with a weight of 5‐6.8 kg. Methods This is a case series study. A bacteriological test, CBC, blood smear, pH, osmotic fragility, 2,3‐diphosphoglycerate (2,3‐DPG), and adenosine triphosphate (ATP) measurement were performed weekly on whole blood (WB) units from day 1 to day 35 after donation. The hemolysis index, lactate and potassium concentrations, prothrombin time (PT), activated partial thromboplastin time (aPTT), and fibrinogen were measured on plasma aliquots. Results One out of eight blood units (BUs) had bacterial growth (Serratia marcescens) at day 35. No significant differences were found regarding CBC, morphology, pH, and osmotic fragility. Despite high inter‐individual variability and low starting levels, significant decreases in the mean concentrations of 2,3‐DPG (T0 1.99 mmol/g Hb, SD 0.52, T35 1.25 mmol/g Hb, SD 1.43; P = .003) and ATP (T0 1.45 mmol/g Hb, SD 0.71, T35 0.62 mmol/g Hb, SD 0.51; P

CD30 is a transmembrane glycoprotein of the tumor necrosis factor receptor superfamily included in the diagnostic algorithm of human cutaneous, anaplastic large cell and Hodgkin lymphomas and represents an optimal therapeutic target for CD30+ tumors. Similar diagnostic and therapeutic approaches are largely missing for feline lymphomas. Cross-reactivity of the antihuman CD30 receptor clone Ber-H2 was investigated in feline lymphomas. Comparative analysis of feline and human CD30 identified 61% identity of the amino acid sequence, with 100% identity of the main sequence of the epitope targeted by the antibody (RKQCEPDYYL). CD30 expression in normal feline tissues was restricted to rare lymphoid cells in perifollicular and interfollicular lymph node areas and in the thymic medulla. In feline lymphoma, CD30 was expressed in 4 of 33 (13%) T-cell lymphomas, 3 of 22 (14%) B-cell lymphomas, and 5 of 7 (71%) mixed-cell lymphomas, showing diffuse (1/5) or multifocal (4/5) positivity restricted to neoplastic multinucleated lymphoid cells and binucleated cells consistent with Reed-Sternberg-like cells. Based on the human classification system, cell morphology, expression of multiple markers (mixed cell components), and CD30 positivity, these cases were considered most consistent with classical Hodgkin-like lymphoma (HLL). The other 2 mixed-cell lymphomas were CD30 negative and thus most consistent with either T-cell-rich large B-cell lymphoma (TCRLBCL) or nodular lymphocyte-predominant Hodgkin lymphoma (NLPHL). These findings provide multiple data supporting the cross-reactivity of the Ber-H2 anti-CD30 clone in feline tissues and give evidence of the usefulness of CD30 in the diagnostic evaluation of feline lymphoma.