13 results on '"Carraro, Roberta"'
Search Results
2. Genomic analysis of Sparus aurata reveals the evolutionary dynamics of sex-biased genes in a sequential hermaphrodite fish
- Author
-
Pauletto, Marianna, Manousaki, Tereza, Ferraresso, Serena, Babbucci, Massimiliano, Tsakogiannis, Alexandros, Louro, Bruno, Vitulo, Nicola, Quoc, Viet Ha, Carraro, Roberta, Bertotto, Daniela, Franch, Rafaella, Maroso, Francesco, Aslam, Muhammad L., Sonesson, Anna K., Simionati, Barbara, Malacrida, Giorgio, Cestaro, Alessandro, Caberlotto, Stefano, Sarropoulou, Elena, Mylonas, Costantinos C., Power, Deborah M., Patarnello, Tomaso, Canario, Adelino V. M., Tsigenopoulos, Costas, and Bargelloni, Luca
- Published
- 2018
- Full Text
- View/download PDF
3. Genetics of resistance to photobacteriosis in gilthead sea bream (Sparus aurata) using 2b-RAD sequencing
- Author
-
Aslam, Muhammad L, Carraro, Roberta, Bestin, Anastasia, Cariou, Sophie, Sonesson, Anna K., Bruant, Jean-Sébastien, Haffray, Pierrick, Bargelloni, Luca, and Meuwissen, Theo H. E.
- Published
- 2018
- Full Text
- View/download PDF
4. Genetic Variation, GWAS and Accuracy of Prediction for Host Resistance to Sparicotyle chrysophrii in Farmed Gilthead Sea Bream (Sparus aurata)
- Author
-
Aslam, Muhammad Luqman, primary, Carraro, Roberta, additional, Sonesson, Anna Kristina, additional, Meuwissen, Theodorus, additional, Tsigenopoulos, Costas S., additional, Rigos, George, additional, Bargelloni, Luca, additional, and Tzokas, Konstantinos, additional
- Published
- 2020
- Full Text
- View/download PDF
5. Genomic prediction of resistance to Photobacterium damselae subsp. piscicida and Sparicotyle chrysophrii in gilthead seabream (Sparus aurata) using 2B-RAD sequencing
- Author
-
Carraro, Roberta
- Subjects
VET/03 Patologia generale e anatomia patologica veterinaria ,Genomic prediction ,Photobacterium damselae subsp. piscicida ,seabream ,Sparicotyle chrysophrii ,2b-RAD ,Genomic prediction, seabream, Photobacterium damselae subsp. piscicida, Sparicotyle chrysophrii, 2b-RAD, real-time PCR ,real-time PCR ,Settore VET/03 - Patologia Generale e Anatomia Patologica Veterinaria - Abstract
Context: Gilthead seabream (Sparus aurata) is a highly important farmed fish species specifically in the Mediterranean aquaculture industry. Infectious diseases present a significant threat to the sustainability of aquaculture with high economic losses due to mortalities, reduced productivity, and the necessity of additional treatments/vaccinations. Specific and sensitive methods for the detection of fish pathogens represent useful tools to investigate infection dynamics and enable early detection of the disease for better prevention. Selection and breeding for resistance against infectious diseases is also a highly valuable tool to help prevent or diminish disease outbreaks, and applying genomic information to the currently available advanced selection methods could accelerate the response to selection. The gram-negative bacteria Photobacterium damselae subsp. piscicida (Phdp) and the ectoparasite Sparicotyle chrisophrii (Sc) are two of the most important pathogens affecting seabream cultivation. Purpose of the study: The aims of this work are: (i) to investigate the genomic prediction of resistance to two highly problematic diseases in seabream through the application of 2b-RAD with the objective of achieving selective breeding goals and (ii) to design an effective assay for the detection and quantification of Phdp. Materials and methods: (i) 1233 and 1001 seabream individuals were challenged trough intramuscular injection with a virulent strain of Phdp and by co-habitation with naturally Sc-infected seabreams, respectively. Animals were monitored daily and data of dead/survived fish and number of parasites in the gills/body length were recorded. Genomic DNA was extracted from the finfish of all individuals and used to construct 2b-RAD libraries. Data were analyzed in order to find SNP based genotypes (GATK, SAMtools), perform Genome-Wide Association Studies, estimate breeding values (ASReml 4.0) and construct linkage-maps (Lep-Map v2). (ii) A primer set was designed from a partial sequence of the bamB gene (Primer3 web) considering two SNPs that discriminate between Phdp and its strictly correlated subspecies Phdd. The assay was tested for specificity/sensitivity on laboratory-generated samples as well as on previous experimentally infected seabream tissue samples. Results and discussion: (i) The reference catalogue contained 175,725 and 269,660 tags for the Phdp and the Sc challenge, respectively. The SNP detection process yielded genotypic data for 19,313 and 21,773 quality SNPs for Phdp and Sc, respectively, both grouped into 24 linkage groups (LG), which are consistent with the karyotype of this species. Genomic heritability for resistance to photobacteriosis was 0.31-0.33 and genomic heritability for tolerance to Sc was 0.11-0.22, suggesting potential to enhance both resistances through family-based selection. Estimated breeding values (EBV) using genomic (GBLUP) information presented 5-43% higher accuracy in comparison to those measured using the only pedigree information (PBLUP). GWAS revealed a quantitative trait locus (QTL) including 7 SNPs at LG17 which presented significant association with resistance to Phdp, while one SNP (LG17) was found affecting tolerance to Sc. (ii) The molecular method proposed for P. damselae diagnosis, with high specificity and sensitivity, proved to be suitable for detection, quantification and subspecies identification in one-step, overcoming the limitations of previous assays. Conclusions: The SNPs discovered through 2b-RAD genotyping could be used to implement new marker-assisted selection programs for the generation of more resistant fish, preventing important disease outbreaks in fish farms. In addition, the original molecular method proposed holds the potential to improve the current knowledge of Phdp infection dynamics and the development of better strategies to control this important fish disease.
- Published
- 2018
6. Additional file 2: of Genetics of resistance to photobacteriosis in gilthead sea bream (Sparus aurata) using 2b-RAD sequencing
- Author
-
Aslam, Muhammad, Carraro, Roberta, Bestin, Anastasia, Cariou, Sophie, Sonesson, Anna, Jean-Sébastien Bruant, Haffray, Pierrick, Bargelloni, Luca, and Meuwissen, Theo
- Abstract
Information on inflation of P-value, LD among significant SNPs, genes at QTL region and Manhattan plots. This file comprises supporting information which contains Q-Q plot, zoom in view of GWAS based P-values distributed at chromosome 2, information on genes around the highest significant SNP of chromsome2, LD values with heatmap for the top 5 significant markers of GWAS analysis, and Manhattan plots showing SNP positions corrected using linkage map information, and the shrinkage of p-values using model with the addition of top significant SNP used as fixed effect. Figure S2.1. Distribution of dead and alive sibs out of total sib count per full-sib family. Figure S2.2. Manhattan plot of –log10 P-values for P_D2D trait distributed across different LGs. Figure S2.3. (A-B): Manhattan plot of –log10 P-values for P_D2D trait distributed along the length (cM) of SA17. Figure S2.4. Q Q-Plot of –log10 P-values – days to death phenotype (P_D2D). Table S2.5. Variances explained by genome-wide significantly associated SNPs of P_D2D trait. Table S2.6. Summary for the functions of important candidate genes at QTL region (DOCX 718 kb).
- Published
- 2018
- Full Text
- View/download PDF
7. Molecular Typing ofVibrio parahaemolyticusStrains Isolated from Mollusks in the North Adriatic Sea
- Author
-
Rahman, Mohammad Shamsur, primary, Carraro, Roberta, additional, Cardazzo, Barbara, additional, Carraro, Lisa, additional, Meneguolo, Davide Boscolo, additional, Martino, Maria Elena, additional, Andreani, Nadia Andrea, additional, Bordin, Paola, additional, Mioni, Renzo, additional, Barco, Lisa, additional, Novelli, Enrico, additional, Balzan, Stefania, additional, and Fasolato, Luca, additional
- Published
- 2017
- Full Text
- View/download PDF
8. Você conhece esta síndrome?
- Author
-
Carraro, Roberta Rodrigues Infante Vieira, Pacheco, Alexsandra da Silva, Zanardi, Daniela, and Souza Filho, Jorge José de
- Subjects
Blepharoplasty ,Pálpebras ,Eyelids ,Dapsona ,Blefaroplastia ,Dermatology ,Dapsone - Abstract
A síndrome de Ascher (SA), um subtipo de0 anetodermia, é rara e caracterizada por apresentar em associação, com maior ou menor expressividade, relaxamento da pele das pálpebras, edema de lábio superior e aumento da tireóide. A etiologia é desconhecida, mas há evidências de defeito nas fibras elásticas, que se apresentam fragmentadas ou diminuídas. Relata-se o caso de paciente de 16 anos de idade, com queixa única: episódios recorrentes de edema bipalpebral. Ascher's Syndrome, a subtype of anetodermia, is a rare disease characterized by the association, in different degrees, of upper eyelid laxity, lip swelling and increase in thyroid size. Its etiology is unknown, but there is evidence for a defect in elastic fibers, which are either fragmented or decreased in number. A case is reported of a 16-year-old patient with a single complaint: recurrent episodes of bilateral eyelid edema.
- Published
- 2006
9. Molecular Typing of Vibrio parahaemolyticus Strains Isolated from Mollusks in the North Adriatic Sea.
- Author
-
Rahman, Mohammad Shamsur, Carraro, Roberta, Cardazzo, Barbara, Carraro, Lisa, Meneguolo, Davide Boscolo, Martino, Maria Elena, Andreani, Nadia Andrea, Bordin, Paola, Mioni, Renzo, Barco, Lisa, Novelli, Enrico, Balzan, Stefania, and Fasolato, Luca
- Published
- 2017
- Full Text
- View/download PDF
10. Molecular Typing of Vibrio parahaemolyticusStrains Isolated from Mollusks in the North Adriatic Sea
- Author
-
Rahman, Mohammad Shamsur, Carraro, Roberta, Cardazzo, Barbara, Carraro, Lisa, Meneguolo, Davide Boscolo, Martino, Maria Elena, Andreani, Nadia Andrea, Bordin, Paola, Mioni, Renzo, Barco, Lisa, Novelli, Enrico, Balzan, Stefania, and Fasolato, Luca
- Abstract
AbstractBackground:Vibrio parahaemolyticusis an emerging foodborne pathogen in the Mediterranean, usually associated with shellfish consumption. The increase in the number of outbreaks in Europe is primarily associated with the global warming of the ocean that has a great impact on the spread and genetic selection of waterborne pathogens. The primary role of Italy in Europe's mollusk production, together with the fact that cases of infections with V. parahaemolyticusare not always notified to the European community, highlighted the necessity of acquiring new information about the epidemiological involvement of shellfish products.Objective:The aim of the study was to provide useful insights into the first steps of the Risk Assessment associated with V. parahaemolyticusthrough the molecular characterization of isolates from commercialized mollusks.Materials and Methods:A total of 102 strains identified as V. parahaemolyticuswere investigated as part of a larger sampling (1-year survey) from several shellfish species collected from the Venice lagoon and the North Adriatic sea. All strains were characterized by multilocus sequence typing and tested for the presence of virulence genes (trhand tdh). The study of sampling/environmental factors and epidemiological analyses was performed to describe the behaviors of the different genetic populations.Results:The population structure analysis highlighted three genetic clusters that could be subject to temperature selection during cold (≤15°C) and warm (>16°C) seasons. Moreover, other factors, such as molluscan species (clams/mussels), probably played a role in the distribution of genetic clusters. Although few strains carried the virulence factors (n= 6 trh+), epidemiological links with clinical isolates and a local dissemination of some sequence types were underlined.Conclusion:This work provides a useful background on the genotype spread as a first step in the Hazard Identification in light of future climate changes.
- Published
- 2017
- Full Text
- View/download PDF
11. Characterization of thyroid nodules by 3-D contrast-enhanced ultrasound imaging
- Author
-
Carraro, Roberta, primary, Molinari, Filippo, additional, Deandrea, Maurilio, additional, Garberoglio, Roberto, additional, and Suri, Jasjit S., additional
- Published
- 2008
- Full Text
- View/download PDF
12. Genetics of resistance to photobacteriosis in gilthead sea bream (<italic>Sparus aurata</italic>) using 2b-RAD sequencing.
- Author
-
Aslam, Muhammad L, Carraro, Roberta, Bestin, Anastasia, Cariou, Sophie, Sonesson, Anna K., Bruant, Jean-Sébastien, Haffray, Pierrick, Bargelloni, Luca, and Meuwissen, Theo H. E.
- Subjects
COMMUNICABLE diseases ,GRAM-negative bacteria ,BACTERIAL genetics ,MEDICAL informatics ,BACTERIA classification ,GENETICS - Abstract
Background: Photobacteriosis is an infectious disease developed by a Gram-negative bacterium
Photobacterium damselae subsp. piscicida (Phdp ), which may cause high mortalities (90–100%) in sea bream. Selection and breeding for resistance against infectious diseases is a highly valuable tool to help prevent or diminish disease outbreaks, and currently available advanced selection methods with the application of genomic information could improve the response to selection. An experimental group of sea bream juveniles was derived from a Ferme Marine de Douhet (FMD, Oléron Island, France) selected line using ~ 109 parents (~ 25 females and 84 males). This group of 1187 individuals represented 177 full-sib families with 1–49 sibs per family, which were challenged with virulentPhdp for a duration of 18 days, and mortalities were recorded within this duration. Tissue samples were collected from the parents and the recorded offspring for DNA extraction, library preparation using 2b-RAD and genotyping by sequencing. Genotypic data was used to develop a linkage map, genome wide association analysis and for the estimation of breeding values. Results: The analysis of genetic variation for resistance againstPhdp revealed moderate genomic heritability with estimates of ~ 0.32. A genome-wide association analysis revealed a quantitative trait locus (QTL) including 11 SNPs at linkage group 17 presenting significant association to the trait withp -value crossing genome-wide Bonferroni corrected thresholdP ≤ 2.22e-06. The proportion total genetic variance explained by the single top most significant SNP was ranging from 13.28–16.14% depending on the method used to compute the variance. The accuracies of predicting breeding values obtained using genomic vs. pedigree information displayed 19–24% increase when using genomic information. Conclusion: The current study demonstrates that SNPs-based genotyping of a sea bream population with 2b-RAD approach is effective at capturing the genetic variation for resistance againstPhdp . Prediction accuracies obtained using genomic information were significantly higher than the accuracies obtained using pedigree information which highlights the importance and potential of genomic selection in commercial breeding programs. [ABSTRACT FROM AUTHOR]- Published
- 2018
- Full Text
- View/download PDF
13. Characterization of thyroid nodules by 3-D contrast-enhanced ultrasound imaging.
- Author
-
Carraro R, Molinari F, Deandrea M, Garberoglio R, and Suri JS
- Subjects
- Algorithms, Contrast Media, Humans, Thyroid Neoplasms blood supply, Thyroid Neoplasms diagnosis, Thyroid Neoplasms diagnostic imaging, Thyroid Nodule blood supply, Thyroid Nodule diagnosis, Ultrasonography, Image Enhancement methods, Thyroid Nodule diagnostic imaging
- Abstract
High resolution ultrasound imaging proved effective in the detection, analysis, and follow-up of thyroid lesions, even if interpretative pitfalls remain. Differentiation between malignant and benign nodules is a major challenge: malignancy is often correlated to an increase in intranodular vasculature, due to rapid angiogenesis. We developed a three-step methodology to reconstruct intranodular vasculature based on 3-D contrast-enhanced images: i) preprocessing; ii) morphological opening by using a structuring element coherent with the microbubbles appearance: iii) thresholding and 3-D representation. We processed the volumes relative to 8 nodules (4 benign and 4 malignant, confirmed by needle biopsy) and we calculated the percentage of intranodular contrast agent. Results confirm that malignant nodules present a higher internal vasculature (52.3% +/- 15.7%) than benign (14.3% +/- 5.3%). Also, two experts reported the reconstructed 3-D volumes and scored the nodules characteristics, obtaining a good agreement with bioptic results. We believe this methodology could be useful to improve nodules differentiation in thyroid ultrasound exams.
- Published
- 2008
- Full Text
- View/download PDF
Catalog
Discovery Service for Jio Institute Digital Library
For full access to our library's resources, please sign in.