43 results on '"Casalini, L"'
Search Results
2. Written and visual cyberbullying victimization in adolescence: Shared and unique associated factors
- Author
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Perasso G., Carone N., Barone L., Celata C., Coppola L., Baggio B., Velasco V., Lavatelli M., Gelmi G., Biffi L., Marella M., Pellegrini C., Perego O., De Agostini W., Benedusi M., Duregon P., Meconi A., Zagheno C., Salinetti M. V., Salada M., Mauri E., Drusetta V., Lise L. A., Giannellini A., Di Cosimo F., Peruzzo L., Rocco T. G., Sala J., Zoppi C., Artioli B., Stampini L., Colombo M., Farina E., Marchesi D., Casalini L., Perasso, G, Carone, N, Barone, L, Celata, C, Coppola, L, Baggio, B, Velasco, V, Lavatelli, M, Gelmi, G, Biffi, L, Marella, M, Pellegrini, C, Perego, O, De Agostini, W, Benedusi, M, Duregon, P, Meconi, A, Zagheno, C, Salinetti, M, Salada, M, Mauri, E, Drusetta, V, Lise, L, Giannellini, A, Di Cosimo, F, Peruzzo, L, Rocco, T, Sala, J, Zoppi, C, Artioli, B, Stampini, L, Colombo, M, Farina, E, Marchesi, D, and Casalini, L
- Subjects
Social Psychology ,05 social sciences ,Developmental and Educational Psychology ,adolescence ,Cybervictimization ,050109 social psychology ,0501 psychology and cognitive sciences ,visual cyberbullying victimization ,Psychology ,HBSC protocol ,written cyberbullying victimization ,050104 developmental & child psychology ,Developmental psychology - Abstract
The present study investigated the antecedents of cyberbullying victimization and addressed the commonalities and differences between visual and written forms of cyberbullying victimization among 3172 Italian adolescents (51.6% male, M age=13.74years, SD=1.70) who participated in the Health Behaviour in School-Aged Children (HBSC, 2014) survey. The results from two logistic regression models revealed that the two distinct forms of cyberbullying victimization presented common and unique associated factors. Family support was negatively associated with both forms of cyberbullying victimization, while greater use of social networks and frequent experiences of traditional bullying victimization were positively associated with both forms. Neither written nor visual forms of cyberbullying victimization were associated with the quality of school relationships or online gaming frequency. Gender (female) was associated with written, but not visual, cyberbullying victimization. Finally, visual cyberbullying victimization was positively associated with high family socio-economic status and traditional bullying perpetration. The findings highlight the urgent need to tailor preventive and intervention strategies for the adolescent population.
- Published
- 2020
- Full Text
- View/download PDF
3. School communication strategies improve schools’ surroundings’ safety: The moderation of students’ participation and anti-bullying programs in northern Italy
- Author
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Perasso, G, Barone, L, Celata, C, Coppola, L, Baggio, B, Velasco, V, Lavatelli, M, Gelmi, G, Biffi, L, Marella, M, Pellegrini, C, Perego, O, De Agostini, W, Benedusi, M, Duregon, P, Meconi, A, Zagheno, C, Salinetti, M, Salada, M, Mauri, E, Drusetta, V, Lise, L, Giannellini, A, Di Cosimo, F, Peruzzo, L, Rocco, T, Sala, J, Zoppi, C, Artioli, B, Stampini, L, Colombo, M, Farina, E, Marchesi, D, Casalini, L, Perasso G., Barone L., Celata C., Coppola L., Baggio B., Velasco V., Lavatelli M., Gelmi G., Biffi L., Marella M., Pellegrini C., Perego O., De Agostini W., Benedusi M., Duregon P., Meconi A., Zagheno C., Salinetti M. V., Salada M., Mauri E., Drusetta V., Lise L. A., Giannellini A., Di Cosimo F., Peruzzo L., Rocco T. G., Sala J., Zoppi C., Artioli B., Stampini L., Colombo M., Farina E., Marchesi D., Casalini L., Perasso, G, Barone, L, Celata, C, Coppola, L, Baggio, B, Velasco, V, Lavatelli, M, Gelmi, G, Biffi, L, Marella, M, Pellegrini, C, Perego, O, De Agostini, W, Benedusi, M, Duregon, P, Meconi, A, Zagheno, C, Salinetti, M, Salada, M, Mauri, E, Drusetta, V, Lise, L, Giannellini, A, Di Cosimo, F, Peruzzo, L, Rocco, T, Sala, J, Zoppi, C, Artioli, B, Stampini, L, Colombo, M, Farina, E, Marchesi, D, Casalini, L, Perasso G., Barone L., Celata C., Coppola L., Baggio B., Velasco V., Lavatelli M., Gelmi G., Biffi L., Marella M., Pellegrini C., Perego O., De Agostini W., Benedusi M., Duregon P., Meconi A., Zagheno C., Salinetti M. V., Salada M., Mauri E., Drusetta V., Lise L. A., Giannellini A., Di Cosimo F., Peruzzo L., Rocco T. G., Sala J., Zoppi C., Artioli B., Stampini L., Colombo M., Farina E., Marchesi D., and Casalini L.
- Abstract
In the last decades, research focused on the surroundings’ influence over schools, but only a few studies investigated whether the presence of a school may increase its surroundings’ safety. Still, the characteristics of the school which could ameliorate the surroundings’ safety are still unclear. The current study hypothesizes that: i. a higher number of communication strategies in the school may increase the school surroundings’ safety; ii. the students’ participation in school decisions and the frequency of anti-bullying programs may strengthen the effect of communication over surroundings’ safety. The sample includes data of 62 school principals from the Northern Italian region of Lombardy who answered self-report questions from Health Behaviors in School-Aged Children (2014) protocol. Findings from moderation analysis show that a higher number of communication strategies within the school fosters the perception of safer school surroundings. The impact of students’ participation in school decisions does not constitute a significant moderator of the relationship. In contrast, the frequency of anti-bullying programs results to impact negatively on the association between communication strategies and surroundings’ safety.
- Published
- 2021
4. Written and visual cyberbullying victimization in adolescence: Shared and unique associated factors
- Author
-
Perasso, G, Carone, N, Barone, L, Celata, C, Coppola, L, Baggio, B, Velasco, V, Lavatelli, M, Gelmi, G, Biffi, L, Marella, M, Pellegrini, C, Perego, O, De Agostini, W, Benedusi, M, Duregon, P, Meconi, A, Zagheno, C, Salinetti, M, Salada, M, Mauri, E, Drusetta, V, Lise, L, Giannellini, A, Di Cosimo, F, Peruzzo, L, Rocco, T, Sala, J, Zoppi, C, Artioli, B, Stampini, L, Colombo, M, Farina, E, Marchesi, D, Casalini, L, Perasso G., Carone N., Barone L., Celata C., Coppola L., Baggio B., Velasco V., Lavatelli M., Gelmi G., Biffi L., Marella M., Pellegrini C., Perego O., De Agostini W., Benedusi M., Duregon P., Meconi A., Zagheno C., Salinetti M. V., Salada M., Mauri E., Drusetta V., Lise L. A., Giannellini A., Di Cosimo F., Peruzzo L., Rocco T. G., Sala J., Zoppi C., Artioli B., Stampini L., Colombo M., Farina E., Marchesi D., Casalini L., Perasso, G, Carone, N, Barone, L, Celata, C, Coppola, L, Baggio, B, Velasco, V, Lavatelli, M, Gelmi, G, Biffi, L, Marella, M, Pellegrini, C, Perego, O, De Agostini, W, Benedusi, M, Duregon, P, Meconi, A, Zagheno, C, Salinetti, M, Salada, M, Mauri, E, Drusetta, V, Lise, L, Giannellini, A, Di Cosimo, F, Peruzzo, L, Rocco, T, Sala, J, Zoppi, C, Artioli, B, Stampini, L, Colombo, M, Farina, E, Marchesi, D, Casalini, L, Perasso G., Carone N., Barone L., Celata C., Coppola L., Baggio B., Velasco V., Lavatelli M., Gelmi G., Biffi L., Marella M., Pellegrini C., Perego O., De Agostini W., Benedusi M., Duregon P., Meconi A., Zagheno C., Salinetti M. V., Salada M., Mauri E., Drusetta V., Lise L. A., Giannellini A., Di Cosimo F., Peruzzo L., Rocco T. G., Sala J., Zoppi C., Artioli B., Stampini L., Colombo M., Farina E., Marchesi D., and Casalini L.
- Abstract
The present study investigated the antecedents of cyberbullying victimization and addressed the commonalities and differences between visual and written forms of cyberbullying victimization among 3172 Italian adolescents (51.6% male, M age = 13.74 years, SD = 1.70) who participated in the Health Behaviour in School-Aged Children (HBSC, 2014) survey. The results from two logistic regression models revealed that the two distinct forms of cyberbullying victimization presented common and unique associated factors. Family support was negatively associated with both forms of cyberbullying victimization, while greater use of social networks and frequent experiences of traditional bullying victimization were positively associated with both forms. Neither written nor visual forms of cyberbullying victimization were associated with the quality of school relationships or online gaming frequency. Gender (female) was associated with written, but not visual, cyberbullying victimization. Finally, visual cyberbullying victimization was positively associated with high family socio-economic status and traditional bullying perpetration. The findings highlight the urgent need to tailor preventive and intervention strategies for the adolescent population.
- Published
- 2021
5. Alcohol consumption in adolescence: the role of adolescents’ gender, parental control, and family dinners attendance in an Italian HBSC sample
- Author
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Perasso, G, Carone, N, Barone, L, Coppola, L, Baggio, B, Velasco, V, Lavatelli, M, Gelmi, G, Biffi, L, Marella, M, Pellegrini, C, Perego, O, De Agostini, W, Benedusi, M, Duregon, P, Meconi, A, Zagheno, C, Salinetti, MV, Salada, M, Mauri, E, Drusetta, V, Lise, LA, Di Cosimo, F, Peruzzo, L, Rocco, TG, Sala, J, Zoppi, C, Artioli, B, Stampini, L, Colombo, M, Farina, E, Marchesi, D, Casalini, L, Perasso, G, Carone, N, Barone, L, Coppola, L, Baggio, B, Velasco, V, Lavatelli, M, Gelmi, G, Biffi, L, Marella, M, Pellegrini, C, Perego, O, De Agostini, W, Benedusi, M, Duregon, P, Meconi, A, Zagheno, C, Salinetti, M, Salada, M, Mauri, E, Drusetta, V, Lise, L, Di Cosimo, F, Peruzzo, L, Rocco, T, Sala, J, Zoppi, C, Artioli, B, Stampini, L, Colombo, M, Farina, E, Marchesi, D, and Casalini, L
- Subjects
alcohol consumption ,05 social sciences ,Attendance ,Sample (statistics) ,Adolescence ,family dinner ,050902 family studies ,gender difference ,0501 psychology and cognitive sciences ,0509 other social sciences ,Psychology ,Alcohol consumption ,Social Sciences (miscellaneous) ,parental control ,050104 developmental & child psychology ,Demography ,Parental control - Abstract
Parental control and family dinners attendance may constitute protective factors against alcohol consumption during adolescence, with different patterns for boys and girls, though evidence thus far have produced mixed findings. The present study analyzed Health Behaviour in School-aged Children (HBSC, 2014) data from 906 adolescents living in Northern Italy (49% boys, Myears = 16.02, SD = 2.4) to examine: (a) gender differences in alcohol consumption frequency; (b) whether greater parental control would mediate gender differences in alcohol consumption over the last 30 days; (c) whether regular attendance of family dinners would strengthen the effect of parental control in decreasing adolescents’ alcohol consumption, functioning differently for boys and girls. Findings indicated that boys reported to attend family dinners more regularly, to consume alcohol more frequently, and to perceive greater paternal control, than girls. Conversely, girls perceived greater maternal control than boys. Both maternal and paternal control did mediate the relation between gender and alcohol consumption, decreasing adolescents’ drinking. Unexpectedly, family dinners attendance did not significantly moderate the effect of parental control on the monthly frequency of adolescents’ alcohol consumption. Results underline the protective role of parental control against adolescents’ alcohol consumption in both girls and boys, regardless of their family dinners attendance.
- Published
- 2021
6. School communication strategies improve schools’ surroundings’ safety: The moderation of students’ participation and anti-bullying programs in northern Italy
- Author
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GIULIA PERASSO, Barone, L., Celata, C., Coppola, L., Baggio, B., Velasco, V., Lavatelli, M., Gelmi, G., Biffi, L., Marella, M., Pellegrini, C., Perego, O., Agostini, W., Benedusi, M., Duregon, P., Meconi, A., Zagheno, C., Salinetti, M. V., Salada, M., Mauri, E., Drusetta, V., Lisè, L. A., Giannellini, A., Di Cosimo, F., Peruzzo, L., Rocco, T. G., Sala, J., Zoppi, C., Artioli, B., Stampini, L., Colombo, M., Farina, E., Marchesi, D., Casalini, L., Perasso, G, Barone, L, Celata, C, Coppola, L, Baggio, B, Velasco, V, Lavatelli, M, Gelmi, G, Biffi, L, Marella, M, Pellegrini, C, Perego, O, De Agostini, W, Benedusi, M, Duregon, P, Meconi, A, Zagheno, C, Salinetti, M, Salada, M, Mauri, E, Drusetta, V, Lise, L, Giannellini, A, Di Cosimo, F, Peruzzo, L, Rocco, T, Sala, J, Zoppi, C, Artioli, B, Stampini, L, Colombo, M, Farina, E, Marchesi, D, and Casalini, L
- Subjects
Principal ,Communication ,School-safety ,Bullying ,Student - Abstract
In the last decades, research focused on the surroundings’ influence over schools, but only a few studies investigated whether the presence of a school may increase its surroundings’ safety. Still, the characteristics of the school which could ameliorate the surroundings’ safety are still unclear. The current study hypothesizes that: i. a higher number of communication strategies in the school may increase the school surroundings’ safety; ii. the students’ participation in school decisions and the frequency of anti-bullying programs may strengthen the effect of communication over surroundings’ safety. The sample includes data of 62 school principals from the Northern Italian region of Lombardy who answered self-report questions from Health Behaviors in School-Aged Children (2014) protocol. Findings from moderation analysis show that a higher number of communication strategies within the school fosters the perception of safer school surroundings. The impact of students’ participation in school decisions does not constitute a significant moderator of the relationship. In contrast, the frequency of anti-bullying programs results to impact negatively on the association between communication strategies and surroundings’ safety.
- Published
- 2021
7. Green School. Una rete di scuole eco–attive per la sostenibilità ambientale in provincia di Varese
- Author
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Casalini, L., Colombo, L., Landini, P., Ruggieri, G., and Sacchiero, P.
- Subjects
green schools ,Educazione ambientale ,SDGS - Published
- 2019
8. An epithermal facility for treating brain gliomas at the TAPIRO reactor
- Author
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Burn, K.W, Casalini, L, Martini, S, Mazzini, M, Nava, E, Petrovich, C, Rosi, G, Sarotto, M, and Tinti, R
- Published
- 2004
- Full Text
- View/download PDF
9. MITOCHONDRIAL DNA GENOTYPING: A NEW TOOL FOR SYNCRONY CANCER DETECTION
- Author
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Guerra, F., Procaccini, M., De Iaco, P., Perrone, A. M., Girolimetti, G., Ceccarelli, C., De Biase, D., Pellegrini, A., Rossi, M., Pozzati, F., Santi, E., Casalini, L., Santini, D., Gasparre, G., Guerra, F., Procaccini, M., De Iaco, P., Perrone, A. M., Girolimetti, G., Ceccarelli, C., De Biase, D., Pellegrini, A., Rossi, M., Pozzati, F., Santi, E., Casalini, L., Santini, D., and Gasparre, G.
- Published
- 2013
10. The epithermal neutron beam for BNCT under construction at TAPIRO: Physics
- Author
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Tinti, R., Nava, E., Casalini, L., Burn, K. W., Tinti, R., Nava, E., Casalini, L., and Burn, K. W.
- Subjects
Applicazioni di fisica e tecnologie nucleari - Abstract
A column to provide an epithermal neutron beam suitable for experimental and clinical BNCT is nearing completion at the TAPIRO reactor (ENEA Casaccia, Rome). TAPIRO is a compact, low power (5 kW), helium-cooled, fast reactor. It has a hard neutron spectrum relative even to other fast reactors. In this paper some of the basic physics aspects of designing an epithermal neutron beam are considered, with reference to the TAPIRO beam.
- Published
- 2006
11. The trade experiment: Shielding calculations for the building hosting the subcritical system
- Author
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Petrovich, C., Nava, E., Monti, S., Casalini, L., Burn, K. W., Petrovich, C., Nava, E., Monti, S., Casalini, L., and Burn, K. W.
- Subjects
Analisi sistemi e di sicurezza - Abstract
The TRADE project (TRiga Accelerator Driven Experiment), to be performed at the existing TRIGA reactor at ENEA Casaccia, has been proposed as a validation of the accelerator-driven system (ADS) concept. TRADE will be the first experiment in which the three main components of an ADS-the accelerator, spallation target and sub-critical blanket-are coupled at a power level sufficient to encounter reactivity feedback effects. As such, TRADE represents the necessary intermediate step in the development of hybrid transmutation systems, its expected outcomes being considered crucial-in terms of proof of stability of operation, dynamic behaviour and licensing issues-for the subsequent realisation of an ADS Transmutation Demonstrator. An essential role in the feasibility study of the experiment is played by radioprotection calculations. Such a system exhibits new characteristics with respect to a traditional reactor, owing to the presence of the proton accelerator. As beam losses always occur under normal operating conditions of an accelerator, shielding studies need to be performed not only around the reactor but also along the beam line from the accelerator to the spallation target. This paper illustrates a preliminary evaluation, using Monte Carlo methods, of the additional shielding to be located around the reactor structures, the beam transport line and the existing reactor building to allow access into the reactor hall and to restrict the doses outside to their legal limits.
- Published
- 2005
12. Comparison between the Monte Carlo codes mcnpx and fluka within the framework of calculations for the trade experiment
- Author
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Rosetti, M., Petrovich, C., Casalini, L., Rosetti, M., Petrovich, C., and Casalini, L.
- Subjects
Fisica del reattore - Abstract
The TRADE experiment, to be performed at the TRIGA nuclear reactor of the ENEA Casaccia Centre (Rome, Italy) consists in the coupling of an external proton accelerator to a spallation target to be installed in the central channel of the reactor kept in a sub-critical configuration. TRADE is meant to represent a major demonstration step of the Accelerator-Driven-Systems concept. Monte Carlo calculations are needed for the radiation transport analysis of various aspects of the experiment. The Monte Carlo general purpose codes MCNPX and FLUKA have been applied, among other issues, to the analysis of the shielding configuration of the beam transport line and to the assessment of the radioactivity induced by the impact of the 140 MeV proton beam with the target. In the shielding analysis the dose rates due to the proton beam leakage along the transport line have been calculated for points beyond the barytic concrete shields. Various leakage angles with respect to the beam axis have been considered. The analysis of the induced radioactivity has involved the calculation of the type and amount of the residual nuclei produced by the spallation process within the tantalum target and of the evolution of such nuclei. The results obtained with MCNPX and FLUKA are compared and discussed.
- Published
- 2005
13. An epithermal facility for treating brain gliomas at the TAPIRO reactor
- Author
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Tinti, R., Sarotto, M., Petrovich, C., Nava, E., Casalini, L., Burn, K. W., Tinti, R., Sarotto, M., Petrovich, C., Nava, E., Casalini, L., and Burn, K. W.
- Subjects
Analisi sistemi e di sicurezza - Abstract
An epithermal facility for treating patients with brain gliomas has been designed and is under construction at the fast reactor TAPIRO at ENEA Casaccia (Italy). The calculational design tools employed were the Monte Carlo codes MCNP/MCNPX together with the DSA in-house variance reduction patch. A realistic anthropomorphic phantom (ADAM) was included to optimise dose profiles and in-phantom treatment-planning figures-of-merit. The adopted approach was to minimise the treatment time whilst maintaining a reasonable therapeutic ratio. It is shown that TAPIRO, in spite of its low power of 5kW, is able to provide an epithermal beam that is of good quality and of sufficient intensity to allow a single beam patient irradiation, under conservative assumptions, of 50min.
- Published
- 2004
14. Design of Neutron Beams for Boron Neutron Capture Therapy in a Fast Reactor
- Author
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Rosi, G., Nava, E., Gambarini, G., Festinesi, A., Burn, K. W., Agosteo, S., Casalini, L., Tinti, R., Rosi, G., Nava, E., Gambarini, G., Festinesi, A., Burn, K. W., Agosteo, S., Casalini, L., and Tinti, R.
- Subjects
Applicazioni di fisica e tecnologie nucleari - Abstract
The BNCT (Boron Neutron Capture Therapy) technique makes use of thermal or epithermal neutrons to irradiate tumors previously loaded with 10B.A thermal neutron beam can be very effective for the treatment of surface tumors such as skin melanoma, but it cannot be used for the treatment of deep pathologies, due to its limited penetration depth. Neutron sources providing an epithermal spectrum of energy ranging from 0.4 eV to 10 keV are being considered for clinical use for the treatment of deep-seated tumors.Reactors are currently seen as a suitable neutron source for BNCT implementation, due to the high intensity of the flux they can provide.The Tapiro reactor, that is located at the ENEA Casaccia Center near Rome, is a low-power fast-flux research reactor that can be useful employed for this application. It is a highly enriched uranium-235 fast neutron facility with a nominal power of 5 kW.In this work computer simulations and experimental arrangements were carried out for this reactor to obtain epithermal and thermal neutron beams for the application of BNCT in Italy in the framework of a specific research program.Using the MCNP-4B code, Monte Carlo calculations were carried out to determine the materials suitable for the design of the thermal and epithermal columns. Various arrangements of reflector and moderator materials have been investigated to achieve the desired experimental conditions.
- Published
- 1999
15. Monte Carlo Technique Applications in the Field of Radiation Dosimetry at the ENEA Radiation Protection Institute: a Review
- Author
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Morelli, B., Gualdrini, G. F., Casalini, L., Morelli, B., Gualdrini, G. F., and Casalini, L.
- Subjects
Applicazioni di fisica e tecnologie nucleari - Published
- 1994
16. Design of an Epithermal Facility for Treating Patients with Brain Gliomas at the TAPIRO Fast Reactor at ENEA Casaccia
- Author
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Burn, K. W., Casalini, L., Daffara, Claudia, Nava, E., Petrovich, C., Gualdrini, G., Fiorani, O., Perrone, A., and Rosi, G.
- Subjects
neutron facility ,MCNP code ,patient treatment - Published
- 2002
17. Control of post-harvest brown rot on nectarine by Epicoccum nigrum and physico-chemical treatments
- Author
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Larena, Inmaculada [0000-0001-8424-8916], De Cal Cortina, Antonieta [0000-0002-7725-7782], Melgarejo, Paloma [0000-0002-3698-8896], Mari, M., Torres, Rosario, Casalini, L., Lamarca, N., Mandrin, J. F., Lichou, J., Larena, Inmaculada, De Cal Cortina, Antonieta, Melgarejo, Paloma, Usall, J., Larena, Inmaculada [0000-0001-8424-8916], De Cal Cortina, Antonieta [0000-0002-7725-7782], Melgarejo, Paloma [0000-0002-3698-8896], Mari, M., Torres, Rosario, Casalini, L., Lamarca, N., Mandrin, J. F., Lichou, J., Larena, Inmaculada, De Cal Cortina, Antonieta, Melgarejo, Paloma, and Usall, J.
- Abstract
Post-harvest physico-chemical treatments - water at 40°C or 60°C (HW), and sodium bicarbonate at 1 or 2.5% (SBC) at 20, 40 and 60°C - were tested on peaches and nectarines in order to control brown rot caused by Monilinia spp.;to select the best physico-chemical treatment to be used in combination with pre-harvest applications of the biological control agent Epicoccum nigrum (ATCC number 96794). Experiments were carried out in France, Italy and Spain in order to test different experimental conditions. Monilinia rot was reduced in peach and nectarine treated with HW (40°C for 2 min) in all three countries, the percentage of rot reduction was similar for France, Italy and Spain (over 40%). A synergistic effect between HW at 40°C during 150 s or at 60°C during 20 s and SBC was observed in all three countries. After preliminary experiments post-harvest treatment of HW (60°C) + SBC (1%) during 20 s was selected to combine it with field treatments. Pre-harvest treatments with fungicide or E. nigrum did not reduce brown rot in any country. However, pre-harvest treatments with E. nigrum, chemical fungicide, and their integration followed by post-harvest physico-chemical treatment significantly reduced Monilinia rot compared to pre-harvest untreated fruits followed by post-harvest physico-chemical treatment in Italy (over 70%). Post-harvest treatments with E. nigrum were also tested in Italy on natural and artificial infections in nectarine over 3 years. E. nigrum, as fresh or formulated cells, at a concentration of 108 conidia mL-1 were effective, significantly reducing the incidence of brown rot compared to control, both under artificial and natural infection, from 43 to 100%. © 2007 Society of Chemical Industry.
- Published
- 2007
18. Efficacy of Candida sake CPA-1 Formulation for Controlling Penicillium expansum Decay on Pome Fruit from Different Mediterranean Regions
- Author
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Torres, R., primary, Teixidó, N., additional, Viñas, I., additional, Mari, M., additional, Casalini, L., additional, Giraud, M., additional, and Usall, J., additional
- Published
- 2006
- Full Text
- View/download PDF
19. The trade experiment: shielding calculations for the building hosting the subcritical system
- Author
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Burn, K. W., primary, Carta, M., additional, Casalini, L., additional, Kadi, Y., additional, Monti, S., additional, Nava, E., additional, Palomba, M., additional, Petrovich, C., additional, Picardi, L., additional, Rubbia, C., additional, and Troiani, F., additional
- Published
- 2005
- Full Text
- View/download PDF
20. Monte Carlo optimisation of a BNCT facility for treating brain gliomas at the tapiro reactor
- Author
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Nava, E., primary, Burn, K. W., additional, Casalini, L., additional, Petrovich, C., additional, Rosi, G., additional, Sarotto, M., additional, and Tinti, R., additional
- Published
- 2005
- Full Text
- View/download PDF
21. Peracetic Acid and Chlorine Dioxide for Postharvest Control of Monilinia laxa in Stone Fruits
- Author
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Mari, M., primary, Cembali, T., additional, Baraldi, E., additional, and Casalini, L., additional
- Published
- 1999
- Full Text
- View/download PDF
22. Benzo(a)pyrene diol-epoxide-DNA adduct levels and aryl hydrocarbon hydroxylase (AHH) in human white blood cells from smokers and non-smokers
- Author
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LODOVICI, V. AKPAN, C. CASALINI, L., M., primary
- Published
- 1999
- Full Text
- View/download PDF
23. Efficacy of Candida sakeCPA-1 Formulation for Controlling Penicillium expansumDecay on Pome Fruit from Different Mediterranean Regions
- Author
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Torres, R., Teixidó, N., Viñas, I., Mari, M., Casalini, L., Giraud, M., and Usall, J.
- Abstract
The effectiveness of a formulated product of the yeast Candida sakeCPA-1 for controlling postharvest diseases on pome fruits was demonstrated in laboratory, semicommercial, and commercial trials carried out in the major pome fruit producing region of the European Union. First, one wettable powder and seven liquid formulations were tested in laboratory trials that involved two varieties of apples and two varieties of pears. In all cases, an efficacy similar to that of fresh cells was demonstrated in the control of artificial Penicillium expansuminfection. After these trials, the formulated product chosen for semicommercial and commercial trials was LF1, a liquid formulation that is particularly suitable for commercial applications. In semicommercial trials, LF1 showed a performance similar to fresh cells in most trials, and the population dynamics of both fresh and formulated cells were quite stable throughout the storage period. This indicates the high viability of C. sakeCPA-1 in this formulation and the absence of adverse effects during the formulation of the product, which may significantly affect both its ability to grow on fruit and its antagonistic activity. We evaluated the control of natural infection after applying the formulated product in a commercial drencher in different packinghouses. A significant reduction in the incidence of diseases was observed with a recommended dose of around 107CFU/ml when natural infections were greater than 1%. In general, large quantities of yeast were observed on the surface of unwounded fruits of different pome fruit cultivars. Moreover, populations of this biocontrol agent increased rapidly on fruit surfaces and remained quite stable for a long time under commercial storage conditions. Commercial practices used in packinghouses were therefore successfully applied for this formulated product.
- Published
- 2006
- Full Text
- View/download PDF
24. Ulteriori sviluppi dello studio per l'automazione della restituzione
- Author
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Bay E., Casalini L., Lombardi S., and Tonazzini A.
- Subjects
Automazione ,Restituzione - Abstract
Questa nota descrive lo stato attuale di uno studio di fattibilità per l'automatizzazione tramite calcolatore, del processo di restituzione fotogrammetrica di riprese con l'ausilio di un proiettore metrico. Tale tipo di riprese, per ora limitate alla fotogrammetria terrestre, è costituita da: 1) un fotogramma ottenuto effettuando un'unica ripresa dell'oggetto, in assenza di luce, dopo avervi proiettato un reticolo piano luminoso; 2) il reticolo stesso (pseudofotogramma) equivalente, in contenuto informativo, ad una seconda ripresa effettuata dal centro di proiezione. La restituzione, in genere, interesserà soltanto i punti dell'oggetto sui quali cadono i nodi del reticolo.
- Published
- 1985
25. The protection of the human embryo in the new Gasparri proposed law
- Author
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P. Grimaldi, A. D'Aloia, L. Montano, G. Falco, S. Guida, S. Landini, L. Valle, F. Di Lella, M. Rizzuti, F. Ferretti, P. Arpaia, A. E. Rizzoli, R. Montanari, R. Presta, M. A. Ciocia, E. Palmerini, A. Bertolini, F. Battaglia, G. Di vetta, P. Haselager, L. Casalini, F. Cesareo, A. Mollo, D. Napolitano, L. M. Sicca, M. Galli, F. Flammini, S. Orlando, V. Cuocci, G. Capilli, L. Viola, G. D'Aietti, A. I. della Valle, R. Paura, L. Gatt, I. A. Caggiano, M. C. Gaeta, E. Troisi, L. Aulino, L. Izzo, D. S. D'Aloia, A. Di Forno, F. Ribezzo, L. Vizzoni., P. Grimaldi, A. D'Aloia, L. Montano, G. Falco, S. Guida, S. Landini, L. Valle, F. Di Lella, M. Rizzuti, F. Ferretti, P. Arpaia, A.E. Rizzoli, R. Montanari, R. Presta, M.A. Ciocia, E. Palmerini, A. Bertolini, F. Battaglia, G. Di vetta, P. Haselager, L. Casalini, F. Cesareo, A. Mollo, D. Napolitano, L.M. Sicca, M. Galli, F. Flammini, S. Orlando, V. Cuocci, G. Capilli, L. Viola, G. D'Aietti, A.I. della Valle, R. Paura, L. Gatt, I.A. Caggiano, M.C. Gaeta, E. Troisi, L. Aulino, L. Izzo, D.S. D'Aloia, A. Di Forno, F. Ribezzo, L. Vizzoni., L. Gatt, M.C. Gaeta, L. Aulino, Grimaldi, P., D'Aloia, A., Montano, L., Falco, G., Guida, S., Landini, S., Valle, L., Di Lella, F., Rizzuti, M., Ferretti, F., Arpaia, P., Rizzoli, A. E., Montanari, R., Presta, R., Ciocia, M. A., Palmerini, E., Bertolini, A., Battaglia, F., Di vetta, G., Haselager, P., Casalini, L., Cesareo, F., Mollo, A., Napolitano, D., Sicca, L. M., Galli, M., Flammini, F., Orlando, S., Cuocci, V., Capilli, G., Viola, L., D'Aietti, G., della Valle, A. I., Paura, R., Gatt, L., Caggiano, I. A., Gaeta, M. C., Troisi, E., Aulino, L., Izzo, L., D'Aloia, D. S., Di Forno, A., Ribezzo, F., and Vizzoni., L.
- Subjects
Vulnerable subjects, reproductive technologies, human embryos, Gasparri law proposal - Abstract
With the bill n. 165 Senator Gasparri returns to the art. 1 c.c. presenting a new proposal – new compared to those dating back to the 16th and 17th legislatures – entitled “Amendment to art. 1 of the civil code in the matter of recognition of the legal capacity of the conceived child”; precisely asking that the recognition of the legal capacity of the fetus already take place at the time of conception and not only after birth, as instead today enshrined in art. 1 of our civil code. A bill that gives the opportunity to reflect, once again, on the questions on the specific theme of the protection of the human embryo and therefore: What is the embryo? What rights should he be recognized? But above all since when should they be recognized?
- Published
- 2023
26. Control of postharvest brown rot on nectarine by Epicoccum nigrum and physico-chemical treatments
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Josep Usall, Inmaculada Larena, Jean Lichou, Maria A De Cal, Lucia Casalini, Jean F Mandrin, Rosario Torres, Marta Mari, N. Lamarca, Paloma Melgarejo, Mari m., Torres R., Casalini L., Lamarca N., Mandrin J.F., Lichou J., Larena I., De Cal M.A., Melgarejo P., and Usall J.
- Subjects
Nutrition and Dietetics ,Sodium bicarbonate ,biology ,EPICOCCUM NIGRUM ,Rosaceae ,HOT WATER ,Biological pest control ,Monilinia ,biology.organism_classification ,Monilinia spp ,MONILINIA SPP ,Conidium ,Fungicide ,BIOCONTROL ,Horticulture ,chemistry.chemical_compound ,chemistry ,Botany ,Postharvest ,POST-HARVEST ,Agronomy and Crop Science ,Epicoccum nigrum ,Food Science ,Biotechnology - Abstract
Post-harvest physico-chemical treatments - water at 40°C or 60°C (HW), and sodium bicarbonate at 1 or 2.5% (SBC) at 20, 40 and 60°C - were tested on peaches and nectarines in order to control brown rot caused by Monilinia spp.;to select the best physico-chemical treatment to be used in combination with pre-harvest applications of the biological control agent Epicoccum nigrum (ATCC number 96794). Experiments were carried out in France, Italy and Spain in order to test different experimental conditions. Monilinia rot was reduced in peach and nectarine treated with HW (40°C for 2 min) in all three countries, the percentage of rot reduction was similar for France, Italy and Spain (over 40%). A synergistic effect between HW at 40°C during 150 s or at 60°C during 20 s and SBC was observed in all three countries. After preliminary experiments post-harvest treatment of HW (60°C) + SBC (1%) during 20 s was selected to combine it with field treatments. Pre-harvest treatments with fungicide or E. nigrum did not reduce brown rot in any country. However, pre-harvest treatments with E. nigrum, chemical fungicide, and their integration followed by post-harvest physico-chemical treatment significantly reduced Monilinia rot compared to pre-harvest untreated fruits followed by post-harvest physico-chemical treatment in Italy (over 70%). Post-harvest treatments with E. nigrum were also tested in Italy on natural and artificial infections in nectarine over 3 years. E. nigrum, as fresh or formulated cells, at a concentration of 108 conidia mL-1 were effective, significantly reducing the incidence of brown rot compared to control, both under artificial and natural infection, from 43 to 100%. © 2007 Society of Chemical Industry.
- Published
- 2007
27. Shielding calculations for a 100 MeV 30 mA proton beam
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K. W. Burn, Carlo Petrovich, Luigi Tecchio, E. Nava, Lodovico Casalini, Tecchio, L., Petrovich, C., Nava, E., Casalini, L., and Burn, k. W.
- Subjects
Applicazioni di fisica e tecnologie nucleari ,Proton ,Fission ,Nuclear Theory ,Monte Carlo method ,Radiation Dosage ,Nuclear physics ,Radiation Protection ,Neutron flux ,Depleted uranium ,Scattering, Radiation ,Radiology, Nuclear Medicine and imaging ,Computer Simulation ,Nuclear Experiment ,Radiometry ,Physics ,Radiation ,Models, Statistical ,Radiological and Ultrasound Technology ,Public Health, Environmental and Occupational Health ,General Medicine ,Equipment Design ,Equipment Failure Analysis ,Italy ,Electromagnetic shielding ,Computer-Aided Design ,Variance reduction ,Particle Accelerators ,Protons ,Monte Carlo Method ,Beam (structure) ,Algorithms - Abstract
The Study and Production of Exotic Species (SPES) project employs a 100 MeV, 30 mA proton beam that strikes a primary target. The resulting high-energy neutron flux impinges on a secondary target of depleted uranium to produce, through fission, beams of short-lived, neutron-rich nuclei. This paper deals with some of the preliminary shielding calculations for the bunker. Monte Carlo is employed with MCNPX and, because of the deep penetrations involved, the in-house variance reduction optimiser, the direct statistical approach. The calculations exhibited a number of typical features that are addressed and discussed. © The Author 2005. Published by Oxford University Press.
- Published
- 2006
28. Monte Carlo optimisation of a BNCT facility for treating brain gliomas at the TAPIRO reactor
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E. Nava, R. Tinti, C. Petrovich, Massimo Sarotto, K. W. Burn, G. Rosi, L. Casalini, Rosi, G., Tinti, R., Sarotto, M., Petrovich, C., Casalini, L., Burn, K. W., and Nava, E.
- Subjects
Applicazioni di fisica e tecnologie nucleari ,Materials science ,Nuclear engineering ,Monte Carlo method ,Boron Neutron Capture Therapy ,Models, Biological ,Nuclear Reactors ,Humans ,Radiology, Nuclear Medicine and imaging ,Computer Simulation ,Radiometry ,Radiation ,Radiological and Ultrasound Technology ,business.industry ,Brain Neoplasms ,Radiotherapy Planning, Computer-Assisted ,Public Health, Environmental and Occupational Health ,Radiotherapy Dosage ,General Medicine ,Glioma ,Brain gliomas ,Neutron capture ,Treatment Outcome ,Italy ,Monte carlo code ,Anthropomorphic phantom ,Variance reduction ,Laser beam quality ,Nuclear medicine ,business ,Monte Carlo Method ,Beam (structure) - Abstract
An epithermal boron neutron capture therapy facility for treating brain gliomas is currently under construction at the 5 kW fast-flux reactor TAPIRO located at ENEA, Casaccia, near Rome. In this work, the sensitivity of the results to the boron concentrations in healthy tissue and tumour is investigated and the change in beam quality on modifying the moderator thickness (within design limits) is studied. The Monte Carlo codes MCNP and MCNPX were used together with the DSA in-house variance reduction patch. Both usual free beam parameters and the in-phantom treatment planning figures-of-merit have been calculated in a realistic anthropomorphic phantom ('ADAM').
- Published
- 2006
29. Effects of early defoliation on shoot photosynthesis, yield components and grape quality
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Poni, Stefano, Casalini, Lorenzo, Bernizzoni, Fabio, Civardi, Silvia, Intrieri, Cesare, PONI S., CASALINI L., BERNIZZONI F., CIVARDI S., and INTRIERI C.
- Subjects
Settore AGR/03 - ARBORICOLTURA GENERALE E COLTIVAZIONI ARBOREE ,berry size - Published
- 2006
30. Biological and molecular characterization of Monilinia laxa from Emilia Romagna
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CASALINI, LUCIA, BARALDI, ELENA, MARI, MARTA, Di Biase L., Montuschi C., Casalini L., Di Biase L., Montuschi C., Baraldi E., and Mari M.
- Subjects
food and beverages ,MONILINIA LAXA ,MONILINIA FRUCTIGENA ,MOLECULAR CHARACTERIZATION ,MONILINIA FRUCTICOLA - Abstract
Brown rot caused by Monilinia sp. is one of the most important diseases of stone fruits. In America, South Africa, Australia and New Zeeland, M: fructicola prevails on M. laxa, whereas in Europe M.laxa, M. fructigena are only the species recorded from stone fruits. M. fructicola is a quarantine pahogen. Brown rot causes serious losses in the orchard and during post harvest. In rainy summers, fungicide treatments fail to reduce losses of susceptible cultivars to acceptable levels. Since 2002 M. fructicolawas recorde from France, a survey carried out to check if this pathogen was present also in Emilia Romagna (Northen Italy). Over 60 fungal isolates were obtained from fruits of sweet cherry, apricolt, peach, nectarine, and plum showing brown rot and smoke grey mycelium typical of M. laxa and M. fructicola. Monoconidial isolates were biologically and genetically characterized by PCR analysis. The biological and morphological charcteristics taken into consideraton were mycelial growth rate and appearance, sporulation, length of conidial germ tube, and fungicide resisitance. The biological characters of 40 fungal isolates were also investigated with in vivo and in vitro trials. All isoaltes were identified as M. laxa Significant differences were observed between isolates with respect to growth and sporulation in vitro and in vivo.
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- 2004
31. Endosposre biocontrol activitiy of Bacillus strains against brown rot on stone fruits: formulation and postharvest application
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CASALINI, LUCIA, MARI, MARTA, Fifi A. P., De Bernardinis M., Ricci M., Casalini L., Mari M., Fifi A.P., De Bernardinis M., and Ricci M.
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BACILLUS ENDOSPORES ,MONILINIA LAXA ,NECTARINES ,POSTHARVEST DISEASES - Published
- 2004
32. Biofumigation for controlling post-harvest fruit pathogens
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Bernardi R., Leoni O., Cinti S., Palmieri S., MARI, MARTA, CASALINI, LUCIA, Bernardi R., Mari M., Leoni O., Casalini L., Cinti S., and Palmieri S.
- Subjects
BRASSICA MEAL ,GLUCOSINALETE ,PENICILLIUM EXPANSUM ,food and beverages ,MONILINIA LAXA ,BIOFUMIGATION - Abstract
Isothiocyanates (ITCs), produced from the catalyzed hydrolisis of glucosinolates conatiend in defatted Brassica meals by endogenous myrosinase, were tested in vivo trials to control two post-harvest pathogens of stone fruits and pears: Monilinia laxa and Penicillium expansum. The aim of this preliminary study was to determine the optimum ITC concentration, temperature, time fo treatment, to control the pathogens avoiding any phytotoxicity on fruit. Before storage, inoculated fruits were treated with ITCs in a cabinet (0.1 mc) changing treatment time and ITC concentration. Using allyl-ITC, pathogens were controlled more than 90% at an average concentration lower than 1 mg/L and a time of fruit treatment of 8 hours. M. laxa appears to be more sensitive than P. expansum to these molecules. In addition, ITCs proved to be activ ealso against thiabendazole resistant strains. The ecouraging results have led to the assembling of a pre-pilot plant for fruits treatment, using ITCs produced from endogenous glucosinolates-myrosinase system of Brassicaceae defatted meal.
- Published
- 2004
33. Evaluation of the efficaciy of different formulations of Candida sake CPA-1 on pome fruits
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Torres R., Teixido N., Giraud M., Usall J., MARI, MARTA, CASALINI, LUCIA, Torres R., Teixido N., Giraud M., Mari M., Casalini L., and Usall J.
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PENICILLIUM EXPANSUM ,BIOCONTROL AGENTS ,POME FRUITS ,CPA-1 ,CANDIDA SAKE - Published
- 2004
34. Twenty years of research on postharvest biological control: results and future prospects
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MARI, MARTA, CASALINI, LUCIA, BERTOLINI, PAOLO, Mari M., Casalini L., and Bertolini P.
- Subjects
POSTHARVEST DISEASES ,BIOLOGICAL CONTROL - Published
- 2004
35. Efficacy of Candida sake CPA-1 formulation for controlling Penicillium expansum decay on pome fruit from different mediterranean reqions
- Author
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Lucia Casalini, Marta Mari, Josep Usall, Rosario Torres, M. Giraud, Immaculada Viñas, Neus Teixidó, Torres R., Teixido N., Vinas I., Mari M., Casalini L., Giraud M., and Usall J.
- Subjects
POSTHARVEST DISEASE ,POME FRUIT ,Population ,Colony Count, Microbial ,Biological pest control ,Microbiology ,Pome ,Food Preservation ,Antibiosis ,media_common.cataloged_instance ,European union ,Pest Control, Biological ,education ,PENICILLIUM EXPASNUM ,Candida ,media_common ,education.field_of_study ,biology ,Mediterranean Region ,Wettable powder ,business.industry ,Penicillium ,food and beverages ,Fungi imperfecti ,biology.organism_classification ,Biotechnology ,BIOCONTROL ,Horticulture ,Fruit ,Food Microbiology ,Postharvest ,Penicillium expansum ,business ,CANDIDA SAKE ,Food Science - Abstract
The effectiveness of a formulated product of the yeast Candida sake CPA-1 for controlling postharvest diseases on pome fruits was demonstrated in laboratory, semicommercial, and commercial trials carried out in the major pome fruit producing region of the European Union. First, one wettable powder and seven liquid formulations were tested in laboratory trials that involved two varieties of apples and two varieties of pears. In all cases, an efficacy similar to that of fresh cells was demonstrated in the control of artificial Penicillium expansum infection. After these trials, the formulated product chosen for semicommercial and commercial trials was LF1, a liquid formulation that is particularly suitable for commercial applications. In semicommercial trials, LF1 showed a performance similar to fresh cells in most trials, and the population dynamics of both fresh and formulated cells were quite stable throughout the storage period. This indicates the high viability of C. sake CPA-1 in this formulation and the absence of adverse effects during the formulation of the product, which may significantly affect both its ability to grow on fruit and its antagonistic activity. We evaluated the control of natural infection after applying the formulated product in a commercial drencher in different packinghouses. A significant reduction in the incidence of diseases was observed with a recommended dose of around 10(7) CFU/ml when natural infections were greater than 1%. In general, large quantities of yeast were observed on the surface of unwounded fruits of different pome fruit cultivars. Moreover, populations of this biocontrol agent increased rapidly on fruit surfaces and remained quite stable for a long time under commercial storage conditions. Commercial practices used in packinghouses were therefore successfully applied for this formulated product.
36. A crossover study on attentional focus and gross motor performance in individuals with Down syndrome.
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Cavaggioni L, Ardigò LP, Castiglioni P, Trecroci A, Casalini L, Formenti D, and Merati G
- Subjects
- Humans, Adult, Male, Female, Young Adult, Down Syndrome physiopathology, Down Syndrome psychology, Attention physiology, Motor Skills physiology, Cross-Over Studies
- Abstract
Little is known about the effect of using an attentional focus instruction on motor performance in people with intellectual disabilities. Therefore, this study explored the effects of different attentional focus instructions on gross motor skill performances in individuals with Down syndrome. Seven community-dwelling participants (age 25.2±3.2 yrs, height 1.70±0.04 m, body mass 72.0±6.3 kg) voluntarily participated in the study. Motor performance on 5-meter running (5m sprint), vertical jump (countermovement jump with arm swing, CMJ), broad jump (standing broad jump, SBJ), forward medball throw (FMBT) or overhead medball backward throw (OMBT) and rising-up from a chair (five repetition sit-to-stand, 5STS) were recorded while performing internal-focus (IF) or external-focus (EF) instructions. EF induced significantly (p<0.05) better performance than IF in CMJ (EF: 15±9 cm; IF: 11±8 cm, median ±interquartile range), SBJ (EF: 0.8±1.05 m; IF: 0.5±1.0 m), FMBT (EF: 1.5±1.4 m; IF: 1.4±1.1 m), OMBT (EF: 4.0±1.5 m; IF: 3.6±1.1 m) and 5STS (EF: 14.2±5.4; IF:15.3±7.7 s). The time over the 5m sprint tended to be shorter with EF (4.0±2.0 s) than IF (5.05±3.3 s) but the difference did not reach the statistical significance (p = 0.29). Physical trainers and school teachers should be encouraged to manage different types of attentional focus instructions to improve cognitive and gross motor performances in persons with Down syndrome., Competing Interests: The authors have declared that no competing interests exist., (Copyright: © 2024 Cavaggioni et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.)
- Published
- 2024
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37. Cyclic di-GMP Signaling Links Biofilm Formation and Mn(II) Oxidation in Pseudomonas resinovorans.
- Author
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Piazza A, Parra L, Ciancio Casalini L, Sisti F, Fernández J, Malone JG, Ottado J, Serra DO, and Gottig N
- Subjects
- Humans, Cyclic GMP metabolism, Oxidation-Reduction, Biofilms, Bacteria metabolism, Bacterial Proteins metabolism, Gene Expression Regulation, Bacterial, Proteomics, Pseudomonas metabolism
- Abstract
Bioaugmentation of biological sand filters with Mn(II)-oxidizing bacteria (MOB) is used to increase the efficiency of Mn removal from groundwater. While the biofilm-forming ability of MOB is important to achieve optimal Mn filtration, the regulatory link between biofilm formation and Mn(II) oxidation remains unclear. Here, an environmental isolate of Pseudomonas resinovorans strain MOB-513 was used as a model to investigate the role of c-di-GMP, a second messenger crucially involved in the regulation of biofilm formation by Pseudomonas, in the oxidation of Mn(II). A novel role for c-di-GMP in the upregulation of Mn(II) oxidation through induction of the expression of manganese-oxidizing peroxidase enzymes was revealed. MOB-513 macrocolony biofilms showed a strikingly stratified pattern of biogenic Mn oxide (BMnOx) accumulation in a localized top layer. Remarkably, elevated cellular levels of c-di-GMP correlated not only with increased accumulation of BMnOx in the same top layer but also with the appearance of a second BMnOx stratum in the bottom region of macrocolony biofilms, and the expression of mop genes correlated with this pattern. Proteomic analysis under Mn(II) conditions revealed changes in the abundance of a PilZ domain protein. Subsequent analyses supported a model in which this protein sensed c-di-GMP and affected a regulatory cascade that ultimately inhibited mop gene expression, providing a molecular link between c-di-GMP signaling and Mn(II) oxidation. Finally, we observed that high c-di-GMP levels were correlated with higher lyophilization efficiencies and higher groundwater Mn(II) oxidation capacities of freeze-dried bacterial cells, named lyophiles, showing the biotechnological relevance of understanding the role of c-di-GMP in MOB-513. IMPORTANCE The presence of Mn(II) in groundwater, a common source of drinking water, is a cause of water quality impairment, interfering with its disinfection, causing operation problems, and affecting human health. Purification of groundwater containing Mn(II) plays an important role in environmental and social safety. The typical method for Mn(II) removal is based on bacterial oxidation of metals to form insoluble oxides that can be filtered out of the water. Evidence of reducing the start-up periods and enhancing Mn removal efficiencies through bioaugmentation with appropriate biofilm-forming and MOB has emerged. As preliminary data suggest a link between these two phenotypes in Pseudomonas strains, the need to investigate the underlying regulatory mechanisms is apparent. The significance of our research lies in determining the role of c-di-GMP for increased biofilm formation and Mn(II)-oxidizing capabilities in MOB, which will allow the generation of super-biofilm-elaborating and Mn-oxidizing strains, enabling their implementation in biotechnological applications.
- Published
- 2022
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38. Role of prognostic scores in predicting in-hospital mortality and failure of non-invasive ventilation in adults with COVID-19.
- Author
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Innocenti F, Lazzari C, Paolucci E, De Paris A, Lagomarsini A, Guerra F, Alleonato P, Casalini L, Buggea M, Caldi F, Zanobetti M, Pieralli F, Guazzini G, Lastraioli L, Luise F, Milia A, Sammicheli L, Maddaluni L, Lavorini F, and Pini R
- Subjects
- Adult, Humans, Male, Middle Aged, Aged, Aged, 80 and over, Female, Respiration, Artificial, Hospital Mortality, Retrospective Studies, Prognosis, Noninvasive Ventilation adverse effects, COVID-19 therapy, Respiratory Insufficiency therapy, Respiratory Insufficiency etiology
- Abstract
We tested the prognostic performance of different scores for the identification of subjects with acute respiratory failure by COVID-19, at risk of in-hospital mortality and NIV failure. We conducted a retrospective study, in the Medical High-Dependency Unit of the University-Hospital Careggi. We included all subjects with COVID-19 and ARF requiring non-invasive ventilation (NIV) between March 2020 and January 2021. Clinical parameters, the HACOR score (Heart rate, Acidosis, Consciousness, Oxygenation, Respiratory Rate) and ROX index ((SpO2/FiO2)/respiratory rate) were collected 3 (-3) and 1 day (-1) before the NIV initiation, the first day of treatment (Day0) and after 1 (+1), 2 (+2), 5 (+5), 8 (+8) and 11 (+11) of treatment. The primary outcomes were in-hospital mortality and NIV failure. We included 135 subjects, mean age 69±13 years, 69% male. Patients, who needed mechanical ventilation, showed a higher HACOR score (Day0: 6 [5-7] vs 6 [6-7], p=.057; Day+2: 6 [6-6] vs 6 [4-6], p=.013) and a lower ROX index (Day0: 4.2±2.3 vs 5.1±2.3, p=.055; Day+2: 4.4±1.2.vs 5.5±1.3, p=.001) than those with successful NIV. An HACOR score >5 was more frequent among nonsurvivors (Day0: 82% vs 58%; Day2: 82% vs 48%, all p<0.01) and it was associated with in-hospital mortality (Day0: RR 5.88, 95%CI 2.01-17.22; Day2: RR 4.33, 95%CI 1.64-11.41) independent to age and Charlson index. In conclusion, in subjects treated with NIV for ARF caused by COVID19, respiratory parameters collected after the beginning of NIV allowed to identify those at risk of an adverse outcome. An HACOR score >5 was independently associated with increased mortality rate., (© 2022. The Author(s).)
- Published
- 2022
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39. Manganese oxidation counteracts the deleterious effect of low temperatures on biofilm formation in Pseudomonas sp. MOB-449.
- Author
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Ciancio Casalini L, Piazza A, Masotti F, Garavaglia BS, Ottado J, and Gottig N
- Abstract
Mn removal from groundwater by biological sand filter technology is negatively impacted by low temperatures in winter periods. Therefore, the need to study Mn(II)-oxidizing bacteria (MOB) having the potential to oxidize Mn(II) and form biofilms at low temperatures is imperative. These MOB can have potential as inocula for sand filter bioaugmentation strategies to optimize Mn removal during winter periods. We previously showed that a Pseudomonas sp. MOB-449 (MOB-449), isolated from a Mn biofilter, oxidizes Mn(II) in a biofilm-dependent way at low temperatures. In this work, MOB-449 Mn(II) oxidation and growth capacities were evaluated under planktonic and biofilm conditions at different temperatures. At 18°C, MOB-449 showed enhanced biofilm formation due to the addition of Mn(II) to the medium correlating with Mn(II) oxidation, compared to biofilms grown in control medium. Moreover, this enhancement on biofilm formation due to the addition of Mn(II) was only observed at 18°C. At this temperature, Mn(II) oxidation in membrane fractions collected from biofilms was induced by uncoupling oxidative phosphorylation from the electron transport chain with 2,4-Dinitrophenol. In Pseudomonas , a role for c-type cytochrome in Mn(II) oxidation has been demonstrated. Accordingly, transcriptional profiles of all terminal oxidases genes found in MOB-449 showed an induction of cytochrome c terminal oxidases expression mediated by Mn(II) oxidation at 18°C. Finally, heme peroxidase activity assays and MS analysis revealed that PetC, a cytochrome c5, and also CcmE, involved in the cytochrome c biogenesis machinery, are induced at 18°C only in the presence of Mn(II). These results present evidence supporting that cytochromes c and also the cytochrome c terminal oxidases are activated at low temperatures in the presence of Mn(II). Overall, this work demonstrate that in MOB-449 Mn(II) oxidation is activated at low temperatures to gain energy, suggesting that this process is important for survival under adverse environmental conditions and contributing to the understanding of the physiological role of bacterial Mn(II) oxidation., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2022 Ciancio Casalini, Piazza, Masotti, Garavaglia, Ottado and Gottig.)
- Published
- 2022
- Full Text
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40. Environmental Bacteria Involved in Manganese(II) Oxidation and Removal From Groundwater.
- Author
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Piazza A, Ciancio Casalini L, Pacini VA, Sanguinetti G, Ottado J, and Gottig N
- Abstract
The presence of iron (Fe) and manganese (Mn) in groundwater is an important concern in populations that use it as source of drinking water. The ingestion of high concentrations of these metals may affect human health. In addition, these metals cause aesthetic and organoleptic problems that affect water quality and also induce corrosion in distribution networks, generating operational and system maintenance problems. Biological sand filter systems are widely used to remove Fe and Mn from groundwater since they are a cost-effective technology and minimize the use of chemical oxidants. In this work, the bacterial communities of two biological water treatment plants from Argentina, exposed to long term presence of Mn(II) and with a high Mn(II) removal efficiency, were characterized using 16S rRNA gene Illumina sequencing. Several selective media were used to culture Mn-oxidizing bacteria (MOB) and a large number of known MOB and several isolates that have never been reported before as MOB were cultivated. These bacteria were characterized to select those with the highest Mn(II) oxidation and biofilm formation capacities and also those that can oxidize Mn(II) at different environmental growth conditions. In addition, studies were performed to determine if the selected MOB were able to oxidize Mn(II) present in groundwater while immobilized on sand. This work allowed the isolation of several bacterial strains adequate to develop an inoculum applicable to improve Mn(II) removal efficiency of sand filter water treatment plants.
- Published
- 2019
- Full Text
- View/download PDF
41. Metabolic profiles of soybean roots during early stages of Fusarium tucumaniae infection.
- Author
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Scandiani MM, Luque AG, Razori MV, Ciancio Casalini L, Aoki T, O'Donnell K, Cervigni GD, and Spampinato CP
- Subjects
- Argentina, Gas Chromatography-Mass Spectrometry, Genotype, Plant Roots metabolism, Glycine max metabolism, Fusarium physiology, Metabolome, Plant Diseases microbiology, Plant Roots microbiology, Glycine max genetics, Glycine max microbiology
- Abstract
Soybean germplasm exhibits various levels of resistance to Fusarium tucumaniae, the main causal agent of sudden death syndrome (SDS) of soybean in Argentina. In this study, two soybean genotypes, one susceptible (NA 4613) and one partially resistant (DM 4670) to SDS infection, were inoculated with F. tucumaniae. Disease symptoms were scored at 7, 10, 14, and 25 days post-inoculation (dpi). The greatest difference in the area under the disease progress curve (AUDPC) values among genotypes was observed at 25 dpi. In order to detect early metabolic markers that could potentially discriminate between susceptible and resistant genotypes, gas chromatography-mass spectrometry (GC-MS) analyses of root samples were performed. These analyses show higher levels of several amino acids and the polyamine cadaverine in the inoculated than in the uninoculated susceptible cultivar at 7 dpi. Principal component analysis (PCA) revealed that the metabolic profile of roots harvested at the earliest time points from the inoculated susceptible genotype was clearly differentiated from the rest of the samples. Furthermore, variables associated with the first principal component were mainly amino acids. Taken together, the results indicate that the pathogen induced the susceptible plant to accumulate amino acids in roots at early time points after infection, suggesting that GC-MS-based metabolomics could be used for the rapid characterization of cultivar response to SDS., (© The Author 2014. Published by Oxford University Press on behalf of the Society for Experimental Biology. All rights reserved. For permissions, please email: journals.permissions@oup.com.)
- Published
- 2015
- Full Text
- View/download PDF
42. Shielding calculations for a 100 MeV 30 mA proton beam.
- Author
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Burn KW, Casalini L, Nava E, Petrovich C, and Tecchio L
- Subjects
- Algorithms, Computer Simulation, Equipment Design, Equipment Failure Analysis, Italy, Monte Carlo Method, Radiation Dosage, Scattering, Radiation, Computer-Aided Design, Models, Statistical, Particle Accelerators instrumentation, Protons, Radiation Protection instrumentation, Radiation Protection methods, Radiometry methods
- Abstract
The Study and Production of Exotic Species (SPES) project employs a 100 MeV, 30 mA proton beam that strikes a primary target. The resulting high-energy neutron flux impinges on a secondary target of depleted uranium to produce, through fission, beams of short-lived, neutron-rich nuclei. This paper deals with some of the preliminary shielding calculations for the bunker. Monte Carlo is employed with MCNPX and, because of the deep penetrations involved, the in-house variance reduction optimiser, the direct statistical approach. The calculations exhibited a number of typical features that are addressed and discussed.
- Published
- 2005
- Full Text
- View/download PDF
43. Biological control of Monilinia laxa and Rhizopus stolonifer in postharvest of stone fruit by Pantoea agglomerans EPS125 and putative mechanisms of antagonism.
- Author
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Bonaterra A, Mari M, Casalini L, and Montesinos E
- Subjects
- Colony Count, Microbial, Food Microbiology, Food Preservation methods, Prunus microbiology, Treatment Outcome, Ascomycota growth & development, Fruit microbiology, Pantoea physiology, Pest Control, Biological, Rhizopus growth & development
- Abstract
Treatment of stone fruits (apricot, peach and nectarine) with Pantoea agglomerans strain EPS125 decreased the incidence and diameter of lesions of brown rot caused by Monilinia laxa and soft rot caused by Rhizopus stolonifer. Root control was achieved on fruits either wounded and subsequently inoculated with the pathogens or non-wounded and naturally infected from orchards. The efficacy of biocontrol was dependent on the concentration of the biocontrol agent and pathogen. At medium to low pathogen dose, optimal EPS125 concentrations were above 10(7) CFU ml(-1). The median effective dose (ED(50)) of EPS125 was 4.5x10(4) in M. laxa and 2.2x10(5) CFU ml(-1) in R. stolonifer. However, EPS125 was more effective in M. laxa than in R. stolonifer as indicated by the ratio between ED(50) of the biocontrol agent and pathogen (K(z)/K(x)) which was 166 and 1263, respectively. Interactions between the strain EPS125 and the fruit surface, and M. laxa and R. stolonifer, were studied to determine the mechanisms of protection from postharvest rots. The strain EPS125 colonizes, grows and survives on stone fruit wounds. Significant inhibition of conidial germination and hyphal growth of R. stolonifer and M. laxa was achieved when the fungal and EPS125 cells were cocultivated on peel leachate or nectarine juice. However, no effect was observed when the antagonist and the pathogen cells were physically separated by a membrane filter which permits nutrient and metabolite interchange. Therefore, a direct interaction between the strain and the pathogen cells is necessary for antagonism, without a significant contribution of the production of antibiotic substances or nutrient competition. Preemptive exclusion by wound colonization and direct interaction with the pathogen is proposed as the mechanism of biocontrol.
- Published
- 2003
- Full Text
- View/download PDF
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