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8 results on '"Castillo-Castañeda, Adriana C."'

2. Diversity and geographical distribution of Leishmania species and the emergence of Leishmania (Leishmania) infantum and L. (Viannia) panamensis in Central-Western Venezuela

Author

Delgado-Noguera, Lourdes A., Hernández-Pereira, Carlos E., Castillo-Castañeda, Adriana C., Patiño, Luz Helena, Castañeda, Sergio, Herrera, Giovanny, Mogollón, Euler, Muñoz, Marina, Duran, Alexander, Loyo, Doris, Pacheco, Mirna, Arena, Luzmir, Isquiel, Glenis, Yepez, Lisbeth, Colmenarez, Beatriz, Caviedes, Mayeli, Mendez, Yamilet, Herrera, Sandry, Ramírez, Juan David, and Paniz-Mondolfi, Alberto E.

Published
2023
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5. Diversity and geographical distribution of Leishmania species and the emergence of Leishmania (Leishmania) infantum and L. (Viannia) panamensis in Central-Western Venezuela

Author

LA, Lourdes A. Delgado-Noguera, primary, Hernández-Pereira, Carlos E., additional, Castillo-Castañeda, Adriana C., additional, Patiño, Luz Helena, additional, Castañeda, Sergio, additional, Herrera, Giovanny, additional, Mogollón, Euler, additional, Muñoz, Marina, additional, Duran, Alexander, additional, Loyo, Doris, additional, Pacheco, Mirna, additional, Arena, Luzmir, additional, Isquiel, Glenis, additional, Yepez, Lisbeth, additional, Colmenarez, Beatriz, additional, Caviedes, Mayeli, additional, Mendez, Yamilet, additional, Herrera, Sandry, additional, Ramírez, Juan David, additional, and Paniz-Mondolfi, Alberto E., additional

Published
2023
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6. Development of an Amplicon-Based Next-Generation Sequencing Protocol to Identify Leishmania Species and Other Trypanosomatids in Leishmaniasis Endemic Areas

Author

Patiño, Luz H., primary, Castillo-Castañeda, Adriana C., additional, Muñoz, Marina, additional, Jaimes, Jesus E., additional, Luna-Niño, Nicolas, additional, Hernández, Carolina, additional, Ayala, Martha S., additional, Fuya, Patricia, additional, Mendez, Claudia, additional, Hernández-Pereira, Carlos E., additional, Delgado, Lourdes, additional, Sandoval-Ramírez, Claudia M., additional, Urbano, Plutarco, additional, Paniz-Mondolfi, Alberto, additional, and Ramírez, Juan David, additional

Published
2021
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7. Identificación de tripanosomátidos a partir de mamíferos silvestres y domésticos en diferentes departamentos de Colombia.

Author

Castillo-Castañeda, Adriana C., Martínez, David, Patiño, Luz H., Muñoz, Marina, Cantillo-Barraza, Omar, Ayala, Martha S., Segura, Maryi, Bautista, Jessica, Urbano, Plutarco, Jaimes-Dueñez, Jeiczon, and David Ramírez, Juan

Abstract

Copyright of Biomédica: Revista del Instituto Nacional de Salud is the property of Instituto Nacional de Salud of Colombia and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Published
2022

8. Clinical performance of a quantitative pan-genus LeishmaniaReal-time PCR assay for diagnosis of cutaneous and visceral leishmaniasis

Author

Ramírez, Juan David, Cao, Liyong, Castillo-Castañeda, Adriana C., Patino, Luz Helena, Ayala, Martha S., Cordon-Cardo, Carlos, Sordillo, Emilia Mia, and Paniz-Mondolfi, Alberto

Abstract

Leishmaniasis is a complex vector-borne disease caused by various Leishmaniaspecies, affecting humans and animals. Current diagnostic methods have limitations, leading to potential misdiagnosis. Therefore, there is an urgent need for specific and sensitive diagnostic tools. We evaluated the sensitivity of a quantitative real-time PCR (qPCR) assay targeting the 18S gene in diverse clinical sample matrices. The assay showed a wide dynamic range and a limit of detection (LoD) of 1 parasite equivalent per milliliter (eq-p/mL) for all tested species. It exhibited high specificity for LeishmaniaDNA, with no amplification against other microorganisms. When applied to samples from patients with visceral and cutaneous leishmaniasis, the qPCR assay provided results that matched the reference methods and allowed estimation of parasite burdens. This assay holds promise for diagnosing and monitoring leishmaniasis by offering high sensitivity, specificity, and the ability to estimate parasitemia. Further studies are needed to enhance Leishmaniamolecular diagnostics and expand their coverage for improved clinical impact.

Published
2023
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