1. CDK10 Mutations in Humans and Mice Cause Severe Growth Retardation, Spine Malformations, and Developmental Delays
- Author
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Sudipto Roy, Noémi van Hul, Janine Altmüller, Hyungwon Choi, Nur'Ain Binte Ali, Xavier Bisteau, Shuhui Lim, Christian Windpassinger, Stéphane Blouin, Verena Rupp, Carine Bonnard, Franz Grill, Byrappa Venkatesh, Bruno Reversade, Rudolf Ganger, Vincenzo Coppola, S. Zakiah A. Talib, Klaus Klaushofer, Majid Alfadhel, Gökhan Yigit, Farid Ben Chehida, Paul Roschger, Ali Al Kaissi, Matias J. Caldez, Umut Altunoglu, Bernd Wollnik, Hülya Kayserili, Lionel Van Maldergem, Alvin Yu Jin Ng, Sameh A. Youssef, Lino Tessarollo, Katharina M. Roetzer, Hao Lu, Philipp Kaldis, Juliette Piard, Alain de Bruin, Sumanty Tohari, Karabey, Hülya Kayserili, Wollnik, Bernd, Kaldis, Philipp, Windpassinger, Christian, Piard, Juliette, Bonnard, Carine, Alfadhel, Majid, Lim, Shuhui, Bisteau, Xavier, Blouin, Stéphane, Ali, Nur'Ain B., Ng, Alvin Yu Jin, Lu, Hao, Tohari, Sumanty, Talib, S. Zakiah A., van Hul, Noémi, Caldez, Matias J., Van Maldergem, Lionel, Yiğit, Gökhan, Youssef, Sameh A., Coppola, Vincenzo, de Bruin, Alain, Tessarollo, Lino, Choi, Hyungwon, Rupp, Verena, Roetzer, Katharina, Roschger, Paul, Klaushofer, Klaus, Altmüller, Janine, Roy, Sudipto, Venkatesh, Byrappa, Ganger, Rudolf, Grill, Franz, Ben Chehida, Farid, Altunoglu, Umut, Al Kaissi, Ali, Reversade, Bruno, School of Medicine, Department of Medical Genetics, Regenerative Medicine, Stem Cells & Cancer, Amsterdam Cardiovascular Sciences, Amsterdam Reproduction & Development (AR&D), Center for Reproductive Medicine, ACS - Heart failure & arrhythmias, and ACS - Diabetes & metabolism
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0301 basic medicine ,Male ,Developmental Disabilities ,medicine.disease_cause ,Mice ,0302 clinical medicine ,Phosphorylation ,Child ,Genetics (clinical) ,Exome sequencing ,Cells, Cultured ,Growth Disorders ,Mice, Knockout ,Mutation ,Cultured ,Cilium ,Cell Cycle ,Disease gene identification ,Phenotype ,Cyclin-Dependent Kinases ,Pedigree ,Embryo ,030220 oncology & carcinogenesis ,Child, Preschool ,Female ,Signal Transduction ,medicine.medical_specialty ,Cells ,Knockout ,Biology ,Article ,03 medical and health sciences ,Germline mutation ,Internal medicine ,Ciliogenesis ,Journal Article ,Genetics ,medicine ,Animals ,Humans ,Star syndrome ,Genome browser ,Protein-kinase ,Cdk10/Cyclin M ,Family ,Gene ,Pisslre ,DNA ,Melanoma ,Member ,Cilia ,Preschool ,Cell Proliferation ,Medicine ,Genetics and heredity ,Mammalian ,Infant ,Fibroblasts ,medicine.disease ,Embryo, Mammalian ,Spine ,030104 developmental biology ,Endocrinology ,Congenital disorder ,Al Kaissi syndrome knockout mice ,CDK10 ,ETS2 ,cilia ,congenital disorder ,growth retardation ,metabolism ,spine malformation - Abstract
In five separate families, we identified nine individuals affected by a previously unidentified syndrome characterized by growth retardation, spine malformation, facial dysmorphisms, and developmental delays. Using homozygosity mapping, array CGH, and exome sequencing, we uncovered bi-allelic loss-of-function CDK10 mutations segregating with this disease. CDK10 is a protein kinase that partners with cyclin M to phosphorylate substrates such as ETS2 and PKN2 in order to modulate cellular growth. To validate and model the pathogenicity of these CDK10 germline mutations, we generated conditional-knockout mice. Homozygous Cdk10-knockout mice died postnatally with severe growth retardation, skeletal defects, and kidney and lung abnormalities, symptoms that partly resemble the disease's effect in humans. Fibroblasts derived from affected individuals and Cdk10-knockout mouse embryonic fibroblasts (MEFs) proliferated normally; however, Cdk10-knockout MEFs developed longer cilia. Comparative transcriptomic analysis of mutant and wild-type mouse organs revealed lipid metabolic changes consistent with growth impairment and altered ciliogenesis in the absence of CDK10. Our results document the CDK10 loss-of-function phenotype and point to a function for CDK10 in transducing signals received at the primary cilia to sustain embryonic and postnatal development., NIH, the National Cancer Institute; Center for Cancer Research; Strategic Positioning Fund for the Genetic Orphan Diseases program; Industry Alignment Fund for the Singapore Childhood Undiagnosed Diseases program from the A*STAR (Agency for Science, Technology, and Research) Biomedical Research Council; A*STAR Biomedical Research Council
- Published
- 2017
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