1. Effect of titanium surface topographies on human bone marrow stem cells differentiation in vitro
- Author
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Vittoria Perrotti, Annalisa Palmieri, Adriano Piattelli, Laura Ricci, Francesco Carinci, Agnese Pellati, Marco Degidi, V. Perrotti, A. Palmieri, A. Pellati, M. Degidi, L. Ricci, A. Piattelli, and F. Carinci
- Subjects
Surface Properties ,Cellular differentiation ,Immunofluorescence ,Dentistry ,Stem cells ,In Vitro Techniques ,Real-Time Polymerase Chain Reaction ,NO ,medicine ,Humans ,Cell culture, Dental implants, Immunofluorescence, Implant topography, Stem cells ,General Dentistry ,DNA Primers ,Titanium ,Base Sequence ,biology ,Chemistry ,business.industry ,Dental implants ,Mesenchymal stem cell ,ALPL ,Cell Differentiation ,Osteoblast ,Middle Aged ,Hematopoietic Stem Cells ,Cell biology ,Implant topography ,RUNX2 ,medicine.anatomical_structure ,Osteocalcin ,biology.protein ,Cell culture ,Implant ,Stem cell ,business - Abstract
Coating characteristics of dental implants such as composition and topography regulate cell response during implant healing. The aim of this study was to assess how surface topography can affect osteogenic differentiation of mesenchymal stem cells (MSCs) by analyzing the expression levels of bone-related genes and MSCs marker. Thirty disk-shaped, commercially pure Grade 2 titanium samples (10 × 2 mm) with 3 different surface topographies (DENTSPLY-Friadent GmbH, Mannheim, Germany) were used in the present study: 10 Ti machined disks (control), 10 Ti sandblasted and acid-etched disks (DPS(®)) and 10 sandblasted and acid-etched disks at high temperature (Plus(®)). Samples were processed for real-time reverse transcription-polymerase chain reaction (RT-PCR) analysis. By comparing machined and Plus(®) disks, quantitative real-time RT-PCR showed a significant reduction of the bone-related genes osteocalcin (BGLAP) and osteoblast transcriptional factor (RUNX2). The comparison between DPS(®) and Plus(®) disks showed a slight induction of all the genes examined (RUNX2, ALPL, COL1A1, COL3A1, ENG, FOSL1, SPP1, and SP7); only the expression of BGLAP remained stable. The present study, demonstrated that implant surface topography affects osteoblast gene expression. Indeed, Plus(®) surface produces an effect on MSCs in the late differentiation stages.
- Published
- 2012
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