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Longhorn beetles (Cerambycidae) are a very diverse group whose species vary greatly in morphology and behaviour, particularly in diel activity. The type genus Cerambyx includes two large oak‐ling sympatric species (Cerambyx cerdo and Cerambyx welensii) that exhibit a crepuscular/nocturnal (dim‐light) lifestyle, but their actual diel activity remains largely unclear.We used phototraps in the wild to assess if diel activity depended on either species, sex or annual meteorology. Phototraps were highly effective, and the imaged activities were representative of beetles' life, including interactions with some vertebrate species.Both longhorns exhibited roughly similar crepuscular/nocturnal behaviour, with daytime activity being usually residual or erratic. Diel activity increased sharply just before dusk, peaked between dusk and early dark night (23:00–2:00 h), and then dropped progressively until disappearing at dawn. Diel activity was sexually dimorphic in C. welensii, as in some years females tended to be active earlier than males.Distinctly, in 2022, the warmest and driest year recorded in the study area, diel activity curves were flatter and wider, with C. cerdo activity increasing in the late afternoon (19:00–21:30 h) and C. welensii in the second half of the night (3:00–6:00 h). Furthermore, both species boosted daytime activity (diurnality) by more than three times, and diurnality also increased over the season that year.We finally hypothesise that, under the current climate change scenario, extreme droughts impact diel activity in these species, particularly boosting diurnality, in an attempt to locate water/food sources to counteract body dehydration. [ABSTRACT FROM AUTHOR]

The pine sawyer beetle (Monochamus galloprovincialis) is a European vector of the invasive pinewood nematode (Bursaphelenchus xylophilus), one of the most dangerous threats to pine forests worldwide. One recommended method for detecting the nematode is catching and inspecting the M. galloprovincialis beetles. Effective commercial lures are available for that insect but attract non‐target species, particularly beneficial Thanasimus species. Our study aimed to develop an equally effective M. galloprovincialis lure less attractive to beneficial predatory insects.We used electroantennography to test several compounds occurring in the pheromones, hindgut extracts or headspace emissions of non‐Ips bark beetles. The compounds that caused the strongest antennal responses were used along with α‐pinene (host‐tree volatile) and monochamol (2‐undecyloxy‐1‐ethanol, a male‐produced aggregation pheromone of M. galloprovincialis) to prepare the lures for olfactometric and field testing.In the field experiments, the lure containing (+)‐α‐pinene, monochamol, (1R)‐(−)‐myrtenal and (S)‐(−)‐trans‐verbenol was equally effective as the commercial lure Galloprotect Pack. It attracted significantly less Thanasimus formicarius and T. femoralis beetles.Thus, the new lure can serve as a basis for developing an operational lure for monitoring M. galloprovincialis with reduced bycatch of predatory beetles. [ABSTRACT FROM AUTHOR]

ABSTRACT The quarantine nematode Bursaphelenchus xylophilus is a causative agent of the pine wilt disease. In Europe, the nematode is vectored by Monochamus galloprovincialis which is also a natural vector of the native, nonpathogenic B. mucronatus. Based on the established regulations, long‐term detection surveys of pine wood and vector insects for the presence of B. xylophilus are obligatory in all EU member countries. The main objective of the presented research was to use the real‐time PCR technique for the fast and precise detection, and identification of Bursaphelenchus nematodes associated with M. galloprovincialis ssp. pistor occurring in Poland. The research conducted on beetles collected in forest stands in Poland revealed exclusive presence of the native, nonpathogenic nematode B. mucronatus. At the beginning of the beetle flight season (June–July), the use of the real‐time PCR allowed detection of B. mucronatus, in the case of its physical presence in the body of the dissected M. galloprovincialis. However, at the end of the flight season (September–October), the DNA of B. mucronatus was also found in some beetles with no physical presence of nematodes in tracheas or under elytra. The univoltine biology of the beetle, gradual decline in dispersal juveniles to zero in/on the beetle body, and positive results of real‐time PCR reaction could indicate that in some of the beetles earlier loaded with nematodes, at the end of the season, only their remains were left after the live nematodes' departure during the vector's maturation feeding and/or egg laying. The obtained results confirm extremely high sensitivity of the real‐time PCR method used in detection and identification of both nematode species which may simultaneously occur in M. galloprovincialis beetles. It allows for precise detection of the nematodes based on their remains left in/on the vector insects. [ABSTRACT FROM AUTHOR]

The article reviews two recent beetle books published by CSIRO Publishing: "Australian Longhorn Beetles volume 3" and "Australian Jewel Beetles." The former focuses on the classification and taxonomy of Prioninae beetles, while the latter emphasizes fieldwork, observation, and photography of jewel beetles. Both books provide valuable information on Australian beetle groups, with the Longhorn Beetles volume 3 covering the Australo-Pacific region and the Jewel Beetles showcasing the beauty and diversity of Buprestidae. The books cater to different audiences, with the Longhorn Beetles volume 3 targeting trained entomologists and the Jewel Beetles appealing to field workers, ecologists, and nature lovers. [Extracted from the article]

Pine wilt disease is one of the most severe and devastating diseases affecting pine forests worldwide, resulting in huge economic losses in many countries. The pinewood nematode (PWN), Bursaphelenchus xylophilus, is the causal agent of pine wilt disease and is obligately vectored by pine sawyer beetles, of the genus Monochamus. For the disease to be present, the habitat must be suitable for the PWN, and include at least one vector species, and at least one host species. To predict its potential distribution, a model must consider all three components. However, no comprehensive study has examined the influence of climatic suitability on the distribution of this “biological complex”. This study addresses this gap by incorporating biotic interactions, specifically involving 13 vectors and 61 host plants, into projections based on the PWN model. We predicted the global potential distribution of pine wilt disease and compared it with the PWN model to highlight the importance of including biotic interactions in species distribution models under climate change. We found that the model revealed an overall trend of increasing suitability scores for both the PWN and pine wilt disease models under future climate scenarios. Furthermore, compared to the PWN model, the biotic model results in an apparent increase in suitability worldwide in the future as the climate will be more suitable to vector and host complexes, suggesting that pine wilt disease could potentially spread to other places via available hosts and vectors. Synthesis and applications. By incorporating biotic interactions, we projected a more accurate suitable area for pine wilt disease, offering valuable insights into regions at high risk for future invasions by the disease and its vectors. This information supports the development of management and early detection strategies in areas of high suitability, helping to mitigate potential economic and ecological losses. Additionally, this study introduces a novel approach for integrating biotic factors into species distribution models. [ABSTRACT FROM AUTHOR]

Simple Summary: Proholopterus chilensis (Cerambycidae: Cerambycinae) is a native species and an important damage agent in the Nothofagaceae forests of southern Chile, significantly affecting timber production in some areas, especially production of Nothofagus obliqua, with no sanitary management measures currently available. Therefore, the present research determined the predisposing factors for male–female sex approaches and identified, for the first time, some compounds that appear to mediate sexual behaviors that could be used to develop specific, safe, and effective control strategies. Interestingly, the chemical communication for mating purposes in P. chilensis preliminarily seems to include both possible "volatile or airborne" pheromones (eventually long-range ones) and short-range pheromonal compounds produced only by females, something, as far as we know, unique in Cerambycinae. During the spring–summer seasons between 2019 and 2023, in the localities of Maquehue (La Araucanía Region) and Llifén (Los Ríos Region), we collected 262 virgin Proholopterus chilensis (1 female/2.3 males) specimens emerging from the live trunks of N. obliqua trees, an atypical sex ratio in Cerambycidae, suggesting high male competition for females. Virgin specimens of both sexes were individually placed in panel traps in the field, capturing only males (n = 184) over the field study seasons and only in traps baited with females. This fact preliminarily suggests the P. chilensis females emit possible "volatile or airborne" pheromones (eventually being a long-range sex pheromone), something unusual in Cerambycinae, the subfamily to which it currently belongs, although the taxonomic status of the species is under debate. In Llifén and Santiago (Metropolitan Region), behavioral observations were conducted, which allowed us to define the conditions necessary for male–female encounters that were replicated when carrying out volatile captures (Head Space Dynamic = HSD) and collections of compounds from terminalias excised from females. In field trials, virgin females less than ten days old were more attractive than older ones and attracted males during the night, i.e., between 23:00 and 5:00 AM, when the ambient temperature exceeded 11.6 °C. The aeration of females under the conditions described above and subsequent analysis of extracts by GC-MS allowed the identification of compounds absent in males and the control, including two oxygenated sesquiterpenes, a nitrogenous compound (C20), and a long-chain hydrocarbon (C26). From the terminalia extracts, hentriacontane, heptacosane, and heneicosyl, heptacosyl and docosyl acetates were identified by GC-MS, and their roles are unknown in the development of short-distance sexual behaviors, but they could serve to mark a trail leading the male towards the female in the final stage of approach for courtship/mating. Thus, we proposed the hypothesis that P. chilensis females emit both a long-range and a trail-pheromone, which, if confirmed, would be a rare case in this family. The specific identity of the compounds obtained by HSD, as well as the activities of these chemicals and those obtained from the terminalias, should be evaluated in future behavioral studies, as well as regarding their potential to attract males under field conditions. The current document is the first report on volatiles obtained from aerations and compounds extracted from female terminalia in P. chilensis. [ABSTRACT FROM AUTHOR]

The recognition of cerambycids as frequent and damaging invaders led to an increase in the interest in the chemical ecology of the group with the identification of pheromones and pheromone-like attractants for well over 100 species. Pheromone components of the Cerambycidae are often phylogenetically conserved, with a single compound serving as a pheromone component for several related species. In the subfamily Lamiinae, the compound 2-(undecyloxy)ethanol (monochamol) has been identified as an aggregation-sex pheromone for several species of the genus Monochamus. In other species, including Monochamus maculosus Haldeman, field trials have demonstrated that monochamol is a pheromone attractant, but at that point it was still unknown as to whether it was a pheromone for this species. Here we report the identification, and laboratory and field trials of a pheromone component produced by adult male M. maculosus. Chemical analyses of headspace volatile collections sampled from field collected beetles of both sexes revealed the presence of one male-specific compound that was identified as 2-(undecyloxy)ethanol. Electroantennography analyses showed that monochamol elicited responses from the antennae of female beetles. Traps baited with monochamol in the field captured M. maculosus adults of both sexes corroborating the identification of monochamol as the sex-aggregation pheromone of this species. The attractivity of monochamol to adult M. maculosus in our field trapping experiment was synergized by the addition of the host volatile α-pinene. [ABSTRACT FROM AUTHOR]

Sustainable forest management faces challenges from various biotic and abiotic stresses. The Asian longhorned beetle (ALB) and drought stress both induce water shortages in poplar trees, but require different management strategies. In northwestern China, ALB and drought stress caused massive mortality in poplar shelterbelts, which seriously affected the ecological functions of poplars. Developing a large-scale detection method for discriminating them is crucial for applying targeted management. This study integrated UAV-hyperspectral and LiDAR data to distinguish between ALB and drought stress in poplars of China's Three-North Shelterbelt. These data were analyzed using a Partial Least Squares-Support Vector Machine (PLS-SVM). The results showed that the LiDAR metric (elev_sqrt_mean_sq) was key in detecting drought, while the hyperspectral band (R970) was key in ALB detection, underscoring the necessity of integrating both sensors. Detection of ALB in poplars improved when the poplars were well watered. The classification accuracy was 94.85% for distinguishing well-watered from water-deficient trees, and 80.81% for detecting ALB damage. Overall classification accuracy was 78.79% when classifying four stress types: healthy, only ALB affected, only drought affected, and combined stress of ALB and drought. The results demonstrate the effectiveness of UAV-hyperspectral and LiDAR data in distinguishing ALB and drought stress in poplar forests, which contribute to apply targeted treatments based on the specific stress in poplars in northwest China. [ABSTRACT FROM AUTHOR]

In the study of yeasts from frass of longhorn beetle larvae, species with high abundance from the genera Diddensiella, Scheffersomyces and Sugiyamaella and some others with lower abundance were observed. The novel ascomycetous yeast species Diddensiella monakovoensis f.a., sp. nov. (holotype: KBP Y-6906; isotypes: VKM Y-3676 and DBVPG 8067) is proposed based on a conventional DNA-barcode sequence analysis and physiological characteristics. The new yeast species differs by 3.5% (11 nt substitutions and 7 indels) in the internal transcribed spacer (ITS region) of the rDNA sequence from the type strain D. caesifluorescens NCAIM Y.01949 T and has about 2% (11 nt substitutions) and 9% (76 nt substitutions and 4 indels) differences from it by sequences of the partial large-subunit (LSU) region and translation elongation factor 1-alpha (TEF1-α) gene, respectively. The genetic differences between the species are also underpinned by different physiology. [ABSTRACT FROM AUTHOR]

Gaps in phylogenetic knowledge are unlikely to be filled in an optimal manner in the absence of a quantitative descriptive framework of phylogenetic coverage to date and a strategy for addressing the remainder (the Darwinian Shortfall). One strategy would be modelling phylogenetic progress on a framework of insect diversity, such as a taxonomic database. I herein sampled existing phylogenetic coverage by collating a machine‐readable tree from each of 1000 insect publications. Processing comprised primarily taxonomic harmonization, the standardization of terminal labels and pruning of uninformative terminal sets such as taxon duplicates. The phylogeny database contained 94,173 unique species IDs over 154,938 terminals in total, with a respective mean and median number of species per phylogeny of 155 and 44. Omics phylogenies contained the most species on average, though not the most novel species, and mitogenome phylogenies contributed the fewest novel species. Synthesis phylogenies were very few in number, but nonetheless predicted to contribute most to increasing phylogenetic coverage of insect diversity. 6.2% of the 970,000 species of the Catalogue of Life were contained amongst the terminals of the database of phylogenies. Phylogenetic coverage of insect families was often disproportionate to species‐richness; those most undersampled were beetles and included families Curculionidae, Staphylinidae, Cerambycidae, and Scarabaeidae, and those with disproportionately high phylogenetic coverage included families of the dragonflies, bees, butterflies and ants. The work herein provides a foundation for quantification of the Darwinian Shortfall, and for shifting to an objective strategy for completing the insect Tree of Life. [ABSTRACT FROM AUTHOR]

Cerambycidae is one of the most diverse and abundant families of wood boring beetles, with 38,583 species described worldwide. One of the main ecological roles of these beetles is the degradation of woody material, but some species are pests that significantly impact agriculture, causing losses of billions of dollars, as well as damage to ecosystems. One species from this family is Placosternus erythropus, the mesquite borer beetle. In this study, we used a metagenomic analysis to determine the bacterial diversity in the intestines of P. erythropus larvae that inhabit Delonix regia trees in Mexico. The most abundant bacterial genera identified were Desulfosarcina, Actibacter, Chlorobaculum, Enterobacter, Sulfurovum, Desulfatiglans, Mycobacterium, Klebsiella, Robiginitalea, Methyloceanibacter, and Filomicrobium, which were members of the phyla Proteobacteria, Actinobacteria, Firmicutes, and Bacteroidetes. Interestingly, bacterial genera that had not been previously found in the Cerambycidae included the following: Desulfuromusa, Desulfosarcina, Sideroxydan, Sulfurovum, Thiobios, Woeseia, Actibacter, Chlorobaculum, Dessulfatiglans, Robiginitalea, Methyloceanibacter and Filomicrobium. [ABSTRACT FROM AUTHOR]

Simple Summary: The main aim of this study was to identify antennally active pheromone components from the citrus long-horned beetle, Anoplophora chinensis, and to test them for behavioral activity. We identified three antennally active male-produced chemical components (nonanal, 4-(n-heptyloxy)butan-1-ol, and 4-(n-heptyloxy)butanal). When tested together in Y-tube behavioral assays, these three compounds were shown to be attractive to adult female A. chinensis. Pheromone production and behavior of A. chinensis are discussed in relation to other closely related Anoplophora cerambycid species. The citrus long-horned beetle, Anoplophora chinensis (Coleoptera: Cerambycidae), is a highly polyphagous species native to eastern and southeastern Asia. Detection of these beetles is dependent on visual surveys, which are inefficient and labor-intensive. The identification and development of pheromone-based lures would help improve survey efforts for A. chinensis should it become established within the US. We identified three antennally active male-produced chemical components (nonanal, 4-(n-heptyloxy)butan-1-ol, and 4-(n-heptyloxy)butanal), which were then tested for behavioral activity at our USDA Quarantine laboratory. When tested together in Y-tube behavioral assays, a three-component blend of the identified compounds was shown to be attractive to adult female A. chinensis. Pheromone production and behavior of A. chinensis and other closely related Anoplophora cerambycid species are discussed in relation to mate finding and potential monitoring. [ABSTRACT FROM AUTHOR]

The use of semiochemical-baited traps for detection, monitoring, and sampling bark beetles and woodboring beetles (BBWB) has rapidly increased since the early 2000s. Semiochemical-baited survey traps are used in generic (broad community level) and specific (targeted toward a species or group) surveys to detect nonnative and potentially invasive BBWB, monitor established populations of invasive or damaging native species, and as a tool to survey natural communities for various purposes. Along with expansion in use, much research on ways to improve the efficacy of trapping surveys for the detection of specific pests as well as BBWB in general has been conducted. In this review, we provide information on intrinsic and extrinsic factors and how they influence the efficacy of detecting BBWB in traps. Intrinsic factors, such as trap type and color, and other factors are described, as well as important extrinsic factors such as habitat selection, horizontal and vertical placement, and disturbance. When developing surveys, consideration of these factors should increase the species richness and/or abundance of BBWB captured in traps and increase the probability of detecting nonnative species that may be present. During generic surveys, deploying more than one trap type or color, using an array of lures, and trapping at different vertical and horizontal positions is beneficial and can increase the number of species captured. Specific surveys generally rely on predetermined protocols that provide recommendations on trap type, color, lure, and trap placement. [ABSTRACT FROM AUTHOR]

The red-necked longhorn beetle, Aromia bungii (Coleoptera: Cerambycidae), is a wood-boring pest of ornamental cherry trees in the Rosaceae family. This study evaluates the effectiveness of a preventive measure using fine-mesh netting in preventing A. bungii from oviposition on host cherry trees. Aromia bungii typically oviposits in cracks and crevices of host tree bark using its elongated ovipositor, leading to higher infestations on the lower trunk where there are abundant rough barks suitable for the beetle oviposition. Therefore, we assessed the effectiveness of netting with a gap that exceeded the reach of the A. bungii ovipositor from the tree surface. This evaluation involved laboratory experiments using cut branches and field experiments using lower trunks from standing trees. In the field, we determined the distribution of A. bungii body sizes and estimated the maximum ovipositor length based on their proportional relationship. This led to the establishment of a 10 mm gap for the test net, featuring a 0.4 mm mesh. Both laboratory and field experiments confirmed that the 0.4 mm mesh net with the specified gap effectively prevented A. bungii oviposition in netted areas. Field experiments also suggested a potential shift in oviposition sites from netted (lower) to un-netted (upper) areas in trees with nets, considering results from trees without nets. Although various measures, such as insecticide spraying, are necessary to safeguard areas without nets, our netting method can serve as a fundamental component of integrated pest management against A. bungii. [ABSTRACT FROM AUTHOR]

The Russian olive (Elaeagnus angustifolia), which functions as a "dead-end trap tree" for the Asian long-horned beetle (Anoplophora glabripennis) in mixed plantations, can successfully attract Asian long-horned beetles for oviposition and subsequently kill the eggs by gum. This study aimed to investigate gum secretion differences by comparing molecular and metabolic features across three conditions—an oviposition scar, a mechanical scar, and a healthy branch—using high-performance liquid chromatography and high-throughput RNA sequencing methods. Our findings indicated that the gum mass secreted by an oviposition scar was 1.65 times greater than that secreted by a mechanical scar. Significant differences in gene expression and metabolism were observed among the three comparison groups. A Kyoto Encyclopedia of Genes and Genomes annotation and enrichment analysis showed that an oviposition scar significantly affected starch and sucrose metabolism, leading to the discovery of 52 differentially expressed genes and 7 differentially accumulated metabolites. A network interaction analysis of differentially expressed metabolites and genes showed that EaSUS1, EaYfcE1, and EaPGM1 regulate sucrose, uridine diphosphate glucose, α-D-glucose-1P, and D-glucose-6P. Although the polysaccharide content in the OSs was 2.22 times higher than that in the MSs, the sucrose content was lower. The results indicated that the Asian long-horned beetle causes Russian olive sucrose degradation and D-glucose-6P formation. Therefore, we hypothesized that damage caused by the Asian long-horned beetle could enhance tree gum secretions through hydrolyzed sucrose and stimulate the Russian olive's specific immune response. Our study focused on the first pair of a dead-end trap tree and an invasive borer pest in forestry, potentially offering valuable insights into the ecological self-regulation of Asian long-horned beetle outbreaks. [ABSTRACT FROM AUTHOR]

The phoretic interaction between Americhernes oblongus (Say) (Chernetidae) and Mallodon chevrolatti (Thomson) (Cerambycidae) is documented for the first time. Furthermore, with this record, the distribution of A. oblongus to western Panama is expanded. This study contributes to our knowledge of the pseudoscorpion fauna in the central part of America, particularly shedding light on phoretic interactions. Americhernes oblongus is now documented for the first time in West Panama, expanding the known distribution within the country. The discovery includes a female specimen found attached to a male Mallodon chevrolatii. This finding not only marks the initial occurrence of A. oblongus on the western side of the isthmus but also represents the first report of a phoretic interaction between these organisms. This study contributes to our knowledge of the pseudoscorpion fauna in the central part of America, particularly shedding light on phoretic interactions. [ABSTRACT FROM AUTHOR]

Rosalia funebris (RFUNE; Cerambycidae), the banded alder borer, is a longhorn beetle whose larvae feed on the wood of various economically and ecologically significant trees in western North America. Adults are short-lived and not known to consume plant material substantially. We sequenced, assembled, and annotated the RFUNE genome using HiFi and RNASeq data. We documented genome architecture and gene content, focusing on genes putatively involved in plant feeding (phytophagy). Comparisons were made to the well-studied genome of the Asian longhorned beetle (AGLAB; Anoplophora glabripennis) and other Cerambycidae. The 814 Mb RFUNE genome assembly was distributed across 42 contigs, with an N50 of 30.18 Mb. Repetitive sequences comprised 60.27% of the genome, and 99.0% of expected single-copy orthologous genes were fully assembled. We identified 12,657 genes, fewer than in the four other species studied, and 46.4% fewer than for Aromia moschata (same subfamily as RFUNE). Of the 7,258 orthogroups shared between RFUNE and AGLAB, 1,461 had more copies in AGLAB and 1,023 had more copies in RFUNE. We identified 240 genes in RFUNE that putatively arose via horizontal transfer events. The RFUNE genome encoded substantially fewer putative plant cell wall degrading enzymes than AGLAB, which may relate to the longer-lived plant-feeding adults of the latter species. The RFUNE genome provides new insights into cerambycid genome architecture and gene content and provides a new vantage point from which to study the evolution and genomic basis of phytophagy in beetles. [ABSTRACT FROM AUTHOR]

This work demonstrates for the first time using molecular markers alongside chromosomes and the intermediate phenotype that a specimen of Dorcadion is an accidental first‐generation hybrid. The analyses were based on two mitochondrial and five nuclear molecular markers. While analysing the putative parental taxa of the hybrid, it was revealed that even Dorcadion lugubre lugubre Kraatz is likely a hybridogenic taxon. Coincidentally, a new lead was discovered concerning the parental taxa of Dorcadion aethiops (Scopoli), already known as a hybridogenic species. This new evidence of hybridisation adds to the recent publications to strengthen the hypothesis that, in Dorcadionini, this phenomenon is relatively frequent, and speciation is often reticulate: New taxa can appear through introgressive hybridisation, explaining in part the extreme species richness of the group. [ABSTRACT FROM AUTHOR]

Increased female age at mating is considered a detrimental factor on reproductive output and fitness in insects, even if the impact is rather species specific. The effect of delayed mating on reproductive output has been widely studied in pest species controlled with mating disruption, as if the method is not fully effective in suppressing matings, it could still delay them, limiting female fitness and pest damage. Female mating delay, however, may also occur in natural habitats without invoking mating disruption. We studied the effect of female delayed mating in Cerambyx welensii (Küster) (Coleoptera: Cerambycidae), an oak‐living sapro‐xylophagous longhorn beetle considered a critical factor in oak decline. Several life history and ecological traits may potentially delay mating, including low abundance, (re)colonisation processes, sexual communication channel, host‐produced kairomones, operational sex ratio, reproductive interference and adverse weather conditions. We conducted laboratory tests to assess the impact of mating delays (0‐, 10‐, 20‐ and 30‐day post‐emergence) on reproductive output. Data showed that mating delay until at least 20 days of age had a limited effect on mating success, lifetime fecundity, longevity and fertility. The daily fecundity pattern depended on mating delay, and virgin females showed ovarian retention. We conclude that C. welensii females have evolved physiological adaptations to overcome mating delays and optimise fitness. We discuss our results from an evolutionary perspective, considering specifically the risk of early predation and egg‐laying time limitation. We hypothesise that unpredictable recurrent stochastic variation in male availability could act as an additional driver selecting for synovigeny in this longhorn species. [ABSTRACT FROM AUTHOR]

Background: Global climate change is causing an increase in extreme high temperatures (EHTs), which subject insects to unprecedented stress. Behavior plasticity in response to EHTs, particularly oviposition behavior, is important for the persistence and outbreak of insect populations. Investigating the plasticity of oviposition behavior and its underlying mechanisms has theoretical importance to pest management, but knowledge gaps still remain. Results: Herein, we characterized the reproductive traits of Monochamus alternatus, a dominant insect vector of the destructive pine wilt disease, including oviposition behavioral patterns, fecundity, offspring fitness and sperm viability, under simulated heatwave conditions in the laboratory. The results showed that (i) EHTs induced a novel oviposition behavior, whereby females deposited multiple eggs into a single groove rather than laying one egg per groove under normal condition; (ii) EHTs exerted stage‐ and sex‐specific effects on fecundity, offspring fitness and sperm viability; and (iii) there was a significant correlation between frequency of the novel oviposition strategy and sperm viability. Conclusion: We hypothesized that this beetle pest has the ability to flexibly shift towards a low‐cost oviposition strategy to counteract the fitness costs caused by heat stress. Taken together, these findings provide a theoretical foundation for personalized pest management strategies in the context of climate change. © 2024 Society of Chemical Industry. [ABSTRACT FROM AUTHOR]

The phoretic interaction between Americhernes oblongus (Say) (Chernetidae) and Mallodon chevrolatti (Thomson) (Cerambycidae) is documented for the first time. Furthermore, with this record, the distribution of A. oblongus to western Panama is expanded. This study contributes to our knowledge of the pseudoscorpion fauna in the central part of America, particularly shedding light on phoretic interactions. Americhernes oblongus is now documented for the first time in West Panama, expanding the known distribution within the country. The discovery includes a female specimen found attached to a male Mallodon chevrolatii. This finding not only marks the initial occurrence of A. oblongus on the western side of the isthmus but also represents the first report of a phoretic interaction between these organisms. This study contributes to our knowledge of the pseudoscorpion fauna in the central part of America, particularly shedding light on phoretic interactions. [ABSTRACT FROM AUTHOR]

The longhorned beetle Rosalia batesi Harold (Coleoptera; Cerambycidae) is endemic to Japan, where its range extends from Hokkaido to Kyushu. The colorful adults are well-known to entomologists and collectors worldwide. It is a hardwood-boring species with larvae that develop in dead broad-leaf trees. In laboratory bioassays, females were attracted to males, which suggested that males produce a sex pheromone. The congeneric species R. alpina is native to Europe, and another congener, R. funebris, is distributed in North America. The pheromone components produced by males of these species had been previously identified as two compounds from different biosynthetic pathways. In the present study, volatiles were collected from beetles of both sexes, and the analyses of the resulting extracts revealed a single male-specific compound, which was identified as 3,5-dimethyl-6-(1-methylbutyl)-pyran-2-one; this is the same compound as the pheromone of the European R. alpina. This alkylated pyrone structure is, so far, unique among known cerambycid pheromones. In field bioassays with traps baited with the racemic synthetic pheromone, significant numbers of both sexes of R. batesi were attracted in an approximately equal ratio, indicating that the compound is an aggregation-sex pheromone rather than a sex pheromone.

Parasitoids of wood borers and bark beetles rarely encounter different host species simultaneously in nature, so whether they exhibit any preferences for particular species, and the relative suitability of different host species is unclear. Sclerodermus guani (Hymenoptera: Bethylidae) is an ectoparasitoid of cerambycid larvae and has also been reported from multiple host species. In this study we used S. guani as a model parasitoid to evaluate preferences for different host species. Moreover, we determined how levels of intraspecific interaction amongst S. guani adults affect host species preference, and subsequent mortality of those different hosts. S. guani adults display high levels of preference for particular hosts and cause rates of non-reproductive mortality of Aromia bungii (Coleoptera: Cerambycidae) and Monochamus alternatus (Cerambycidae) larvae that are higher than the reproductivity mortality of both hosts. Under different parasitoid densities interaction, S. guani always preferred A. bungii and Monochamus saltuarius (Cerambycidae) and varied in its responses to Thyestilla gebleri (Cerambycidae) and M. alternatus. In addition, the mortalities of single T. gebleri, A. bungii or M. saltuarius larvae caused by the parasitoids (1–3 individuals) were all 100%. These results can help us to better understand S. guani host choice behavior and its biological control potential. [ABSTRACT FROM AUTHOR]

Saproxylic beetles are in the spotlight of conservation biology. Crete (Greece) constitutes a special biogeographical entity and an endemism hotspot for many organisms. Despite its poor forest coverage and the continuous degradation of Cretan forests by human activities, Crete hosts an impressive assemblage of saproxylic Coleoptera, some of them being endemic of the island or/and threatened under the I.U.C.N. criteria. Herein we present new data regarding several saproxylic Coleoptera species of Crete, reporting the first records of five species for the Cretan entomofauna [Calais parreysii (Steven, 1829), Dorcus parallelipipedus (Linnaeus, 1758), Dorcus peyronis Reiche and Saulcy, 1856, Platypus cylindrus (Fabricius, 1792) and Echinocerus floralis (Pallas, 1773)].

Structural color originates from the interference of light with periodic structures that feature characteristic length scales on the order of the wavelength of visible light. Long‐range order in photonic structures usually causes iridescence, and increasing disorder renders colors angle‐independent. Random disorder distributes scattering intensity over all wavelengths, producing white in the absence of absorption. Various non‐iridescent, vivid color patterns are found in Sternotomini longhorn beetles. Herein, Sternotomis virescens is investigated, where elytral scales produce a green‐blue color pattern on otherwise black elytra. Combining focused ion beam scanning electron microscopy (FIB‐SEM) tomography, ultra‐small‐angle X‐ray scattering (USAXS), structural modeling, and full‐wave optical simulations, it is found that the color originates from amorphous photonic networks based on sub‐units resembling the I‐WP unit cell, a triply‐periodic minimal surface with body‐centered‐cubic symmetry. This work provides insights into how quasi‐order produces stable colors in S. virescens longhorn beetles, highlights the advantages of volumetric imaging using FIB‐SEM tomography of porous nanostructured materials, and raises interesting questions about the formation mechanisms of amorphous structures in vivo. [ABSTRACT FROM AUTHOR]

The order Chaetothyriales encompasses black yeasts and related fungi known for their diverse morphologies, asexual reproduction, and habitat preferences. In this study, we isolated two fungal strains belonging to Chaetothyriales, designated KNUF-23-13A and KNUF-22-020, from a longhorn beetle (Cyrtoclytus capra) and soil sample, respectively, in Korea. Phylogenetic analyses based on the concatenated nucleotide sequences of the internal transcribed spacer regions and partial sequences of the large subunit (LSU) rRNA, β-tubulin (TUB2), and actin (ACT) genes revealed that the strains were grouped with strains of Trichomerium and Exophiala, respectively. For strain KNUF-23-13A, cultural and morphological differences were observed in the shape and size of mycelium between it and its phylogenetically closest species, Trichomerium lapideum CGMCC 3.17311T, supporting the novelty of this isolated species. In the case of strain KNUF-22-020, the cultural and morphological characteristics were similar to those previously reported for E. radicis P2854T. Based on morphological and phylogenetic analyses, we revealed KNUF-23-13A as a novel species, which we describe and name T. koreanum sp. nov., and KNUF-22-020 as Exophiala radicis, a previously unreported species in Korea. [ABSTRACT FROM AUTHOR]

The longhorn beetle Graphisurus fasciatus (Degeer) ranges from southeastern Canada to Florida and west to Texas, and has frequently been caught during field trials testing attraction of other cerambycid species to their synthesized pheromones. Collections of headspace volatiles from live beetles revealed that males but not females produce a polyketide compound identified as (4R,6S,7E,9E)-4,6,8-trimethylundeca-7,9-dien-3-one ([4R,6S,7E,9E]-graphisurone). Field trials verified that beetles of both sexes were attracted to the synthesized compound, indicating that it is an aggregation-sex pheromone. This structure represents a new structural motif among cerambycid pheromones, and a new natural product. While this study was in progress, the same compound was isolated from males of the South American cerambycid Eutrypanus dorsalis (Germar), in the same subfamily (Lamiinae) and tribe (Acanthocinini) as G. fasciatus. Field trials in Brazil confirmed that (4R,6S,7E,9E)-graphisurone is also an aggregation-sex pheromone for E. dorsalis, and a possible pheromone for two additional sympatric lamiine species, Hylettus seniculus (Germar) (Acanthocinini) and Oreodera quinquetuberculata (Drapiez) (tribe Acrocinini). These results indicate that graphisurone may be shared among a number of related species, as has been found with many components of cerambycid pheromones. [ABSTRACT FROM AUTHOR]

This document serves as a diagnostic protocol for Aromia bungii, commonly known as the red necked longhorn beetle. The beetle is originally from East Asia but has been introduced to Japan and Europe. It primarily infests Prunus species and is identified through signs such as larval frass accumulation, exit holes, and the presence of adults. Molecular tests are recommended for identification, especially when morphological examination is not possible. The document also provides a description and identification guide for Aromia bungii, including information on other similar species and details on larvae differentiation. It emphasizes the use of molecular methods for confirmation, particularly in new outbreaks, and offers guidelines for reporting and documentation. The document acknowledges the contributions of various individuals and organizations in its development and provides reference material and sequences for further research. [Extracted from the article]

Anoplophora chinensis Forster (Coleoptera: Cerambycidae) is a highly polyphagous and invasive species native to East Asia that makes tunnels in the stems of a variety of broad-leaved plant species and cultivated plant species, as well as citrus and hazelnut. In this study, we collected A. chinensis from hazelnut orchards in Sakarya province of Türkiye, and subsequently recovered the parasitoid Megaselia scalaris (Diptera: Phoridae) from dead adult beetles. The present study reports M. scalaris as a new natural enemy of A. chinensis, and more study is needed on this species' behaviour and biological parameters as a parasitoid. [ABSTRACT FROM AUTHOR]

Simple Summary: Longhorn beetles of the genus Monochamus are vectors of the pine wood nematode, which causes incurable pine wilt disease in coniferous trees. We developed temperature-dependent models to describe the oviposition of Monochamus saltuarius, a significant species within this genus. Our study demonstrated that these models effectively capture the nonlinear effects of temperature on the oviposition and aging of M. saltuarius adults. We compared two simulation approaches: one that separately accounted for the sexual maturation phase of adults and another that did not. Both methods proved capable of accurately describing oviposition patterns across various temperature conditions. The insights gained from this study have potential applications beyond estimating oviposition and predicting population dynamics of M. saltuarius. This modeling framework could be adapted to forecast population changes in a diverse range of pest species, offering a versatile tool for pest management strategies. Monochamus saltuarius Gebler is a serious insect pest in Europe and East Asia regions, including Portugal, Spain, China, Japan, and Korea. It transfers the pine wood nematode Bursaphelenchus xylophilus to conifer trees, resulting in pine wilt disease (PWD). As temperature is a key factor influencing insect population dynamics, temperature-dependent models describing M. saltuarius oviposition could estimate population growth potential and evaluate outbreak risks. In this study, the longevity and fecundity of M. saltuarius females were measured under constant temperature conditions ranging from 20 to 32 °C, and temperature-dependent models were constructed. The longevity of M. saltuarius females ranged from 83.36 days to 22.92 days, with a total fecundity of 141 eggs and 52.77 eggs at 20 °C and 32 °C, respectively. To describe oviposition, we used a single-phase simulation describing oviposition as a single model and a two-phase simulation describing sexual maturation and oviposition as two separate models. These models effectively described M. saltuarius oviposition (r2 > 0.96) under constant temperature conditions, with the two-phase simulation demonstrating greater accuracy overall. Such models could facilitate assessments of PWD risks. The modeling framework of this study shows potential for predicting threats from various forestry and agricultural pests. [ABSTRACT FROM AUTHOR]

In 2009, we determined the effects of the enantiomeric composition of the kairomone, α-pinene, on trap catches of arboreal beetles (Coleoptera) in stands of eastern pine trees with resin dominated by (+)-α-pinene. We hypothesized that the responses of beetles would correlate with the predominant enantiomer of α-pinene found in host pines. Lures of (+)-, racemic (±), and (−)-α-pinene were added separately to ethanol-baited multiple-funnel traps. Species such as Monarthrum mali (Fitch), Dendroctonus terebrans (Olivier), Ips grandicollis (Eichhoff), and Pachylobius picivorus (Germar) (Coleoptera: Curculionidae) showed a preference for traps co-baited with (–)-α-pinene. α-Pinene enhanced attraction of Hylastes salebrosus Eichhoff, Hylastes porculus Erickson and Hylastes tenuis Eichhoff (Coleoptera: Curculionidae) to ethanol-baited traps with no effects from enantiomeric composition of α-pinene. The attraction of the ambrosia beetles, Xyleborinus saxesenii (Ratzeburg) and Dryoxylon onoharaense (Murayama) (Coleoptera: Curculionidae) to ethanol-baited traps was interrupted by the addition of α-pinene, regardless of enantiomeric composition. Species such as Xylosandrus germanus (Blandford), Cnestus mutilatus (Blandford) and Stenoscelis brevis (Boheman) (Coleoptera: Curculionidae) were unaffected by the presence of α-pinene. Trap catches of some species of longhorn beetles and bark beetle predators (Coleoptera: Cerambycidae, Cleridae, Elateridae, Histeridae, and Trogossitidae) were increased by the addition of α-pinene, although results varied by location. Platysoma spp. (Coleoptera: Histeridae) showed a marked preference for traps co-baited with (+)-α-pinene in Florida and Georgia. In summary, we found that the enantiomeric composition of α-pinene in hosts was not a good predictor of enantiomeric preferences by beetles. [ABSTRACT FROM AUTHOR]

The Mongolian oak forest is a natural deciduous forest with a large distribution area in the Changbai Mountains. Clarifying the distribution pattern of longhorn beetles is of scientific value for conservation and monitoring of some species. Flight intercept traps were used to collect longhorn beetles from the canopy and ground level of the ridge, southern slope and northern slope of the Mongolian oak forest in Changbai Mountains. Topographical position and vertical position had no interactive effect on the distribution of longhorn beetle richness and abundance, but topographical positions and vertical position independently affected the composition of the beetle assemblages. The community composition of longhorn beetles in the forest canopy and at ground level was significantly different, and that on the ridge and the southern slope was significantly different from that on the northern slope. Richness and abundance were higher in the canopy than at ground level, and were higher on the ridge and the southern slope than on the northern slope. In the process of species conservation and monitoring, the spatial distribution of longhorn beetles in forests should be considered for trap location. [ABSTRACT FROM AUTHOR]

Two leafy liverwort species from the late Eocene Rovno amber are described. They are placed in the extant genus Nipponolejeunea (Jubulaceae, Porellales, Marchantiophyta) that includes two extant East Asian species and one fossil species described from the Eocene Baltic amber. New fossil species N. rovnoi sp. nov. and N. solodovnikovii sp. nov. differ from the Baltic N. europea, thus making the extinct diversity of the genus exceeding the contemporary species diversity, and such a case is registered for the first time for liverworts. The finding of Nipponolejeunea in Rovno amber may indicate the presence of Abies in the Eocene of Ukraine, since at the southern border of its distribution in Japan extant species the genus grows only on fir trunk. This is in a good concordance with the recent finding in Rovno amber the longhorn beetle Poliaenus europaeus, also associated with fir trees. [ABSTRACT FROM AUTHOR]

Monochamus alternatus is one of the most important borers of conifers and the main vector of the pinewood nematode, Bursaphelenchus xylophilus, the causal agent of pine wilt disease. It causes massive death of pine trees and seriously affects the health of forest ecosystems. Traditionally, adults or larvae are identified by morphological or molecular methods. However, diagnosing M. alternatus larvae at different instars collected from forests is expensive and time‐consuming. Non‐destructive molecular diagnostic protocols are, therefore, being developed to detect biological traces (i.e. exuviae, excreta) and to determine the distribution and spread of this pest. In this study, based on the alignment of mitochondrial DNA cytochrome oxidase subunit I (COI) gene sequences of M. alternatus and other insect species, we designed the primer pair of Mal‐SF/Mal‐SR and probe of Mal‐P for M. alternatus. TaqMan probe‐based qPCR was developed to identify the occurrence of M. alternatus in forests by amplifying the DNA samples obtained from its adult, larva, frass, excreta and exuviae. The amplification results were very effective. The lowest amount of M. alternatus DNA that could be detected with a Cq of 31.93 in the mixed samples was 0.64 pg, showing very high sensitivity. This assay can easily identify M. alternatus from other non‐target wood‐borer species using its frass and exuviae, providing a new diagnostic protocol for monitoring the occurrence and distribution of M. alternatus in forests. [ABSTRACT FROM AUTHOR]

Resolving a robust phylogeny of an organismal group is often hindered by the limited availability of samples suitable for genomic or transcriptomic sequencing. Even for lineages of notable importance in evolutionary ecology, our phylogenetic comprehension remains largely unsatisfactory due to the challenges of acquiring samples across the clade. The long‐horned beetle genus Anoplophora Hope exemplifies such a group, globally renowned for two invasive pests—the Asian long‐horned beetle and citrus long‐horned beetle—which have inflicted significant damage to deciduous hardwood forest in North America and Europe. In contrast to the two temperate pests, the remaining 50 species in the genus inhabit subtropical forests of Southeast Asia, where most species are only infrequently encountered. Here, we present the first comprehensive phylogeny of Anoplophora using a PCR‐based target enrichment museomics approach. As a case study of employing PCR‐generated custom probes, we demonstrate the robustness and cost‐effectiveness of this in‐house method in successfully acquiring sequence data from historical specimens. Through extensive sampling of Anoplophora using museum specimens, we reveal a non‐sister relationship between the two temperate species and provide evidence for addressing taxonomic conundrums. Our biogeographical analyses indicate that the adaptation of the two temperate species occurred independently during the late Pliocene and Pleistocene after the establishment of temperate forests in East Asia in the late Miocene. Our findings highlight the importance of comprehensive phylogenetic inference in understanding the patterns and processes of these beetles' adaptation to temperate forests and lay the groundwork for investigating the genetic mechanism underlying life in the cold. [ABSTRACT FROM AUTHOR]

Sentinel gardens are effective in assessing the risk of insect or pathogen invasions on plants from offshore regions. Limited knowledge exists on wood borers' potential impact on expatriate plants and host stress levels. We surveyed wood borers colonizing 11 North American tree species planted in two sentinel gardens in China, recording 38 species from 9 families, mainly Curculionidae (Scolytinae), Cerambycidae, and Cossidae. Quercus texana was the most attacked host. Wood borers typically infest unhealthy or dead tissues, regardless of overall tree vitality. We identified species capable of colonizing healthy trees: Anoplophora chinensis (Forster) (Cerambycidae), Sinoxylon sp. (Bostrichidae), and Polyphagozerra coffeae (Nietner) (Cossidae). Several insects colonizing stressed trees included: Xylosandrus crassiusculus (Motschulsky), Xyleborus affinis (Eichhoff), Xylosandrus germanus (Blandford) (all Curculionidae-Scolytinae), and Chlorophorus sp. (Cerambycidae). Some, such as A. chinensis and P. coffeae, are under-documented but potentially invasive pests with considerable regulatory importance. Most recorded species are already recognized as pest of live trees or invasive elsewhere, indicating a limited number of potentially invasive wood borers rather than an inexhaustible pool. This suggests that sentinel gardens are effective for pest prevention prioritization. We recommend three methodological improvements for pre-invasion assessments: 1) documenting the vitality of specific colonized tissues to avoid misrepresenting secondary colonizers as primary pests, 2) using fully digital data-management from tree planting to insect identification, and 3) routinely identifying pests using DNA. [ABSTRACT FROM AUTHOR]

Castanea dentata (American Chestnut) was likely an important floral resource for a variety of insects in pre-blight eastern forests, including the rare oligolectic bee Andrena rehni, and insects may have played a significant role in the tree's pollination. We surveyed 2 chestnut orchards in New York in 2023 for insect visitors to male catkins. We recorded 66 species and retrieved pollen samples from 49 specimens across 6 families. Andrena rehni was documented after a 119-year absence in the state, and for all specimens, only Castanea pollen was detected in their pollen loads. Honeybees, bumblebees, and sweat bees also intentionally collected chestnut pollen, while longhorn beetles and soldier flies had the highest rates of carrying chestnut pollen incidentally. Our findings support the existing idea that some insects, like beetles, have the potential to contribute to chestnut pollination, and that American Chestnut provides pollen and nectar to a diversity of insects in northeastern landscapes. [ABSTRACT FROM AUTHOR]

The need for industrially and biotechnologically significant enzymes, such as phytase, is expanding daily as a result of the increased use of these enzymes in a variety of operations, including the manufacture of food, animal feed, and poultry feed. This study sought to characterize purified phytase from A. awamori AFE1 isolated from longhorn beetle for its prospect in industrial applications. Ammonium sulfate precipitation, ion-exchange chromatography, and gel-filtration chromatography were used to purify the crude enzyme obtained from submerged fermentation using phytase-producing media, and its physicochemical characteristics were examined. The homogenous 46.8-kDa phytase showed an 8.1-fold purification and 40.7% recovery. At 70 C and pH 7, the optimum phytase activity was noted. At acidic pH 4–6 and alkaline pH 8–10, it likewise demonstrated relative activity of 88–95% and 67–88%, respectively. It showed 67–70% residual activity between 30 and 70 C after 40 min, and 68–94% residual activity between pH 2 and 12 after 2 h. The presence of Hg+, Mg2+, and Al3+ significantly decreased the enzymatic activity, whereas Ca2+ and Cu2+ enhanced it. Ascorbic acid increased the activity of the purified enzyme, whereas ethylenediaminetetraacetic acid (EDTA) and mercaptoethanol inhibited it. The calculated values for Km and Vmax were 55.4 mM and1.99 μmol/min/mL respectively. A. awamori phytase, which was isolated from a new source, showed unique and remarkable qualities that may find use in industrial operations such as feed pelleting and food processing. [ABSTRACT FROM AUTHOR]

Two new genera and two species of Desmiphorini are described from Mexico: Evrysoma cinereumgen. n., sp. n. from Querétaro and San Luis Potosí; Kuklimia balamgen. n., sp. n. from Chiapas. Diagnostic characters of the new species are discussed and photographs of the type material are provided. A key to Mexican genera of Desmiphorini is provided. [ABSTRACT FROM AUTHOR]

Ochrocesis jakli sp. nov. from Laos (Houaphanh) is described. The habitus and male genitalia are illustrated. Ochrocesis cyaneoapicalis Hayashi, 1982 is newly confirmed from the type locality in Peninsular Malaysia. The habitus of male specimen and male genitalia are also illustrated. [ABSTRACT FROM AUTHOR]

Trichorondonia kabateki sp. nov. from China (Sichuan) is described and illustrated. [ABSTRACT FROM AUTHOR]

As part of a concerted research effort to design management strategies for an economic insect pest of cashew (Anacardium occidentale L.), viz., Analeptes trifasciata Fabricius (Coleoptera: Cerambycidae), also known as the stem girdler, it became necessary to understand the biotic and abiotic factors influencing its population fluctuation. The population dynamics of A. trifasciata and its relationship with crop phenology (flushing, senescence, flower initiation, fruit development and maturation stages) and weather factors (average temperature, relative humidity and rainfall) were investigated from 2017 to 2019 at the cashew orchard of Cocoa Research Institute of Nigeria (CRIN), Ibadan, Nigeria. Observations were recorded at monthly intervals for the duration. The period of incidence was from May to December. Peak abundance, which coincided with the wet season as well as senescence and flower initiation stages, were not significantly correlated. Adult abundance of A. trifasciata was conspicuously low during the fruit development and maturation stages, this stage coincided with the peak of the dry season. However, there was no significant correlation between the pest abundance and crop phenological stages. The male to female ratio was 1:0.81 (÷2 =1.313, df =1, p =0.252). The abundance of A. trifasciata significantly but negatively correlated (r = -0.73) with temperature. Relative humidity (r = 0.30) and rainfall (r = 0.24) showed non-significant association with stem-girdler population. Our findings indicated that temperature is the major predictor of A. trifasciata population fluctuation irrespective of the phenological stage. These findings can be useful for developing a forecasting model for its early detection and appropriate management. [ABSTRACT FROM AUTHOR]

The introduction of wood-boring insects to non-native areas can pose a significant threat to global forest ecosystems. Aromia bungii and Massicus raddei, native to Asia including Korea but potentially important in urban forestry worldwide, are severe trunk borers that have recently infested host trees in urban landscapes of Korea. The signs of external injury can help identify cerambycid-infested trees. However, assessing the internal condition of trees without causing damage is challenging, which hinders the management of infested trees that are still alive. This study evaluated the potential use of sonic tomography to assess injury characteristics in the woody parts of trees, such as Prunus × yedoensis and Quercus palustris infested by A. bungii and M. raddei, respectively. Among the 45 investigated trees, the number of holes on external parts was recorded by visual examination. Sonic tomography images revealed decayed areas, allowing for a comparison of internal damage between infested and non-infested trees. The internal status of trees infested by the two cerambycid beetles differed from that of healthy trees. While some healthy trees exhibited minimal internal damage, those that were not infested by beetles showed decayed areas, potentially caused by non-biological factors such as frozen cracks. In conclusion, sonic tomography equipment can potentially be used for non-invasive assessment of internal injuries caused by wood-boring insects in urban trees. [ABSTRACT FROM AUTHOR]

Copyright of Acta Zoologica Lilloana is the property of Fundacion Miguel Lillo and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

In forest ecosystems, fires profoundly impact the habitat and seasonal dynamics of numerous Coleoptera species. The phenology of species and communities is particularly noteworthy in burned areas that were previously forest ecosystems. In 2023, research was conducted in the Mordovia State Nature Reserve on 11 burned plots in 2021 and control plots. The research employed the use of beer traps, which are baited with a combination of beer and sugar. In the second year after the fires, species from the families Nitidulidae, Cerambycidae, Scarabaeidae, and Elateridae exhibited the highest total number across all plots, accounting for 84.8% of all Coleoptera specimens collected. A single peak in the abundance of Coleoptera was observed on all plots during the month of May. The second peak in abundance during the autumn season was relatively modest in scale and was observed exclusively on unburned 2021 plots. Nitidulidae beetles appear in traps earlier than other beetles, with their number peaking in April and May. Thereafter, their number declined in June. The number of Cerambycidae and Scarabaeidae on all plots was relatively low in April but increased significantly in May and June. The peak abundance of Elateridae was observed in June, although the first individuals were recorded in April. A precipitous decline in the abundance of the species was observed in July, with only a single specimen being recorded in August. The Elateridae were observed to be particularly prevalent in burned areas where deadwood was present. There are variations in the observed differences between individual families, which are related to the location of plots, the condition of the vegetation cover, and the presence of flowering plants, deadwood, and dry trees on the plots. [ABSTRACT FROM AUTHOR]

Copyright of R.A.R.E - Association Roussillonnaise d'Entomologie is the property of Association Roussillonnaise d Entomologie and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Baited traps are a basic component of both specific and generic surveillance programs targeting wood-boring beetles at risk of introduction to new habitats because of global trade. Among the numerous protocols developed over the years for generic surveillance of longhorn beetles, jewel beetles, and bark and ambrosia beetles is the simultaneous use of black multi-funnel traps set up in the understory and green multi-funnel traps set up in the canopy of forested areas surrounding ports and other entry points. These traps are commonly baited with multi-lure blends of pheromones and host volatiles. In this study, we tested this trapping protocol in areas surrounding eight entry points located in Europe and North America to determine: i) the relative performance of black-understory traps and green-canopy traps among the targeted taxa; and ii) whether the dissimilarity among communities of beetles collected by the understory vs. canopy traps was affected by taxon and amount of forest cover in the traps' surroundings. A total of 96,963 individuals belonging to 358 species of wood-boring beetles were collected, including 21 non-native species. Black-understory multi-funnel traps were generally more efficient than green-canopy multi-funnel traps for detecting longhorn beetles and bark and ambrosia beetles, whereas the opposite trend was observed for jewel beetles. Differences between beetle communities caught in black-understory and green-canopy traps were mainly attributed to differences in species richness in jewel beetles, while both differences in species richness and species turnover contributed to the dissimilarity between communities of longhorn beetles and bark and ambrosia beetle. The difference in the number of jewel beetle species caught by the two trapping methodologies decreased with increasing forest cover, whereas species turnover increased when moving from an urban-dominated to a forest-dominated landscape. Overall, these results suggest that the simultaneous use of both black-understory and green-canopy multi-funnel traps can be considered a very efficient approach for generic surveillance of longhorn beetles, jewel beetles and bark and ambrosia beetles in both urban-dominated and forest-dominated areas surrounding entry points. [ABSTRACT FROM AUTHOR]

Four species of Cerambycidae (Coleoptera) are recorded for the first time for Guinea: Neoplocaederus viridipennis (Hope, 1843), N. iridescens (Atkinson, 1953), N. glabricollis (Hope, 1843) and Olenecamptus triplagiatus Jordan, 1894. Habitus photographs and distribution maps of the four species are included. [ABSTRACT FROM AUTHOR]

Entomopathogenic fungi (EPFs) are a significant group of insect pathogens that are used as microbial insecticides with distinct physiological mechanisms of pathogenicity. During our survey in the middle of March 2020 to early October 2022 on natural EPF as a biological control agent of the longhorn beetle (LHB) Osphranteria coerulescens, new isolates of EPFs were found on LHB larvae in apple orchards of Dargaz, Razavi Khorasan province, Iran. The nBLAST results of this these indigenous isolate indicated the highest similarity to Cladosporium sp. (Ascomycota: Davidiellaceae) with ON307222.1 access number and for EF-1α indicated the highest similarity to Cladosporium sp. with MH724951.1 access number. To assess the virulence of these indigenous isolates of EPF, a series of tests were conducted on the LHB larvae stages. Conidial concentrations 104, 105, 106, 107, and 108 (conidia/ml− 1) caused (10.21 ± 2.1), (23.44 ± 2.4), (28.72 ± 2.6), (33.23 ± 3.1) and (34.02 ± 2.8) % of mortality in LHB, respectively and there was no significant difference between 108 and 107 (conidia/ml−1) concentrations. The mortality results by the time showed that the process did not start until 3 days after treatment, and after that, it sped up. This research gathers new information about cryptic inhabiting larvae which shows considerable potential for this Cladosporium species to be implemented within the microbial control program of the LHB. This research is a new report isolating the pathogenicity of Cladosporium sp. on LHB. These fungal isolates have considerable potential for the microbiological control of the LHB. [ABSTRACT FROM AUTHOR]

Insects belong to the Arthropoda phylum and are the most numerous animal group in the world, with more than 1.000.000 species. Insects ensure the sustainability of life on earth by pollinating plants, improving soil texture by decomposing organic materials, and providing nutrients to humans. DNA isolation is a necessity for performing analyses such as Sanger sequencing, DNA fingerprinting, and gene-genome analysis. Isolated insect DNA samples can be used to carry out studies such as species identification, taxonomic relations, comparison of insect-plant and -microfauna relations, revealing genomic, population, and genetic diversity, bio-geography determination, combating agricultural pests, etc. In this study, it is aimed to compare three different isolation methods in terms of time and quality for obtaining genetic material from museum samples (old) and new samples of insects. The samples used in this investigation belong to the same insect species of Cerambycidae family (Coleoptera). Samples collected at most 9-12 months ago are considered as "new specimen", whereas "old specimens" consist samples of 7-28 years old. Three DNA isolation protocols: Phenol-Chloroform, Salting Out and DArT seq, are compared in this study. Considering quality gel images and A260/280 and A260/230 values in newly collected samples, the best results in terms of quality were obtained with the phenol-chloroform method. When A260/280 values were examined alone, DArT seq protocol appears to be the most appropriate protocol in terms of purity in both old and new insect samples. In terms of time, the Phenol-chloroform method was found to be the shortest protocol lasting for about 2 h. Accordingly, the phenol-chloroform method was deduced to be the better choice for both old and new insect samples. After concentration adjustments, the obtained DNAs can be used for the next experimental stages or stored as stock material at -20 °C. Determining the appropriate protocol to obtain high-quality DNA in a short time is important for the continuation of the studies. Our findings are important in terms of accelerating the studies for identification and determining systematic status and especially in examining the taxonomic relationships of the insect species. Using molecular taxonomy in addition to classical taxonomy allows the identification of insects on a species basis. These types of studies will generate useful information for researchers who study insect molecular biology. [ABSTRACT FROM AUTHOR]