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The drive to sleep is strongly influenced by time of day, with temporal information conveyed through the circadian clock. In pursuit of the neural mechanisms underlying this process, in this issue of Neuron , Sun et al. identify a novel circuit that links circadian output neurons to sleep-promoting neurons within the mushroom bodies. [ABSTRACT FROM AUTHOR]

Drosophila melanogaster's large lateral ventral neurons (lLNvs) are part of both the circadian and sleep-arousal neuronal circuits. In the past, electrophysiological analysis revealed that lLNvs fire action potentials (APs) in bursting or tonic modes and that the proportion of neurons firing in those specific patterns varies circadianly. Here, we provide evidence that lLNvs fire in bursts both during the day and at night and that the frequency of bursting is what is modulated in a circadian fashion. Moreover, we show that lLNvs AP firing is not only under cell autonomous control, but is also modulated by the network, and in the process we develop a novel preparation to assess this. We demonstrate that lLNv bursting mode relies on a cholinergic input because application of nicotinic acetylcholine receptor antagonists impairs this firing pattern. Finally, we found that bursting of lLNvs depends on an input from visual circuits that includes the cholinergic L2 monopolar neurons from the lamina. Our work sheds light on the physiological properties of lLNvs and on a neuronal circuit that may provide visual information to these important arousal neurons. [ABSTRACT FROM AUTHOR]

Drosophila melanogaster is a model organism that has been instrumental in understanding the circadian clock at different levels. A range of studies on the anatomical and neurochemical properties of clock neurons in the fly led to a model of interacting neural circuits that control circadian behavior. Here we focus on recent research on the dynamics of the multiple communication pathways between clock neurons, and, particularly, on how the circadian timekeeping system responds to changes in environmental conditions. It is increasingly clear that the fly clock employs multiple signalling cues, such as neuropeptides, fast neurotransmitters, and other signalling molecules, in the dynamic interplay between neuronal clusters. These neuronal groups seem to interact in a plastic fashion, e.g., rearranging their hierarchy in response to changing environmental conditions. A picture is emerging supporting that these dynamic mechanisms are in place to provide an optimal balance between flexibility and an extraordinary accuracy. [ABSTRACT FROM AUTHOR]

ABSTRACT Circadian rhythms are conserved across kingdoms and coordinate physiology and behavior for appropriate time-keeping. The neuronal populations that govern circadian rhythms are described in many animal models, and the current challenge is to understand how they interact to control overt rhythms, remaining plastic enough to respond and adapt to a changing environment. In Drosophila melanogaster, the circadian network comprises about 150 neurons, and the main synchronizer is the neuropeptide pigment-dispersing factor (PDF), released by the well-characterized central pacemaker neurons, the small ventral lateral neurons (sLNvs). However, the rules and properties governing the communication and coupling between this central pacemaker and downstream clusters are not fully elucidated. Here we genetically manipulate the speed of the molecular clock specifically in the central pacemaker neurons of Drosophila and provide experimental evidence of their restricted ability to synchronize downstream clusters. We also demonstrate that the sLNv-controlled clusters have an asymmetric entrainment range and were able to experimentally assess it. Our data imply that different clusters are subjected to different coupling strengths, and display independent endogenous periods. Finally, the manipulation employed here establishes a suitable paradigm to test other network properties as well as the cell-autonomous mechanisms running in different circadian-relevant clusters. J. Comp. Neurol. 523:982-996, 2015. © 2014 Wiley Periodicals, Inc. [ABSTRACT FROM AUTHOR]

The neuropeptide pigment-dispersing factor (PDF) synchronizes molecular oscillations within circadian pacemakers in the Drosophila brain. It is expressed in the small ventral lateral neurons (sLNvs) and large ventral lateral neurons, the former being indispensable for maintaining behavioral rhythmicity under free-running conditions. How PDF circuits develop the specific connectivity traits that endow such global behavioral control remains unknown. Here, we show that mature sLNv circuits require PDF signaling during early development, acting through its cognate receptor PDFR at postsynaptic targets. Yet, axonal defects by PDF knockdown are presynaptic and become apparent only after metamorphosis, highlighting a delayed response to a signal released early on. Presynaptic expression of constitutively active bone morphogenetic protein (BMP) receptors prevents pdfr mutants misrouting phenotype, while sLNv-restricted downregulation of BMP signaling components phenocopied 01- Thus, we have uncovered a novel mechanism that provides an early "tagging" of synaptic targets that will guide circuit refinement later in development. [ABSTRACT FROM AUTHOR]

In this paper a source apportionment of particulate matter pollution in the urban area of Milan (Italy) is given. Results of PM10 and PM2.5 mass and elemental concentrations from a 1-year monitoring campaign are presented. Mean annual and daily PM10 levels are compared with the limits of the EU Air Quality Directive EC/30/1999 and the results show that the limit values established would not be met in the urban area of Milan or the large surrounding area. Moreover, high levels of PM2.5 are registered and this fraction constitutes a high portion of the PM10 mass. In Milan the winter period is characterised by a high degree of air pollution due to a greater contribution of emissions and to adverse meteorological and thermodynamic conditions of the atmosphere. The application of multivariate techniques and receptor modelling (PCFA, APCFA) to the whole data-set led to the identification of the main emitting sources and to the source apportionment of PM10 and PM2.5 in Milan. The most important sources were identified as ‘soil dust’, ‘traffic’, ‘industry’ and ‘secondary compounds’ for PM10 and as ‘soil dust’, ‘anthropogenic’ and ‘secondary compounds’ for PM2.5, explaining the greatest part of the total variance (91% and 75%, respectively). [Copyright &y& Elsevier]

Presents a study where CRYPTOCHROME (CRY), a protein involved in circadian photoperception in Drosophila, is shown to block the function of PERIOD/TIMELESS (PER/TIM) heterodimeric complexes in a light-dependent fashion. Uncoupling of TIM degradation from abrogation of its function by light; Direct interaction of CRY and TIM in yeast in a light-dependent fashion; Influence of PER/TIM and CRY on the subcellular distribution of these protein complexes; Conclusion that CRY act as a circadian photoreceptor.

A new resistance-breaking isolate of potato virus X (PVX MS; also called PVX fca01) that multiplies in genotypes carrying the Rx gene was detected in Argentina. Enzyme-linked immunosorbent assay (ELISA) assessment of PVX multiplication in mechanically inoculated micropropagated plantlets revealed that PVX MS is able to replicate in both resistant (Rx) and susceptible plantlets whereas strain PVX cp is able to replicate only in the latter. Immunoblot detection with specific monoclonal antibodies showed that PVX MS belongs to the serotype PVXo (common or European strain), whereas PVX HB is included in the serotype PVXA (Andean). SDS polyacrylamide gel electrophoresis followed by immunoblotting of the coat proteins of the PVX strains cp, cp4, HB, and MS showed that they differed in mobility with apparent mol, wts of 27.9, 28.8, 29.9, and 29.4 kDa, respectively. [ABSTRACT FROM AUTHOR]

Rhythmic rest-activity cycles are controlled by an endogenous clock. In Drosophila, this clock resides in ;150 neurons organized in clusters whose hierarchy changes in response to environmental conditions. The concerted activity of the circadian network is necessary for the adaptive responses to synchronizing environmental stimuli. Thus far, work was devoted to unravel the logic of the coordination of different clusters focusing on neurotransmitters and neuropeptides. We further explored communication in the adult male brain through ligands belonging to the bone morphogenetic protein (BMP) pathway. Herein we show that the lateral ventral neurons (LNvs) express the small morphogen decapentaplegic (DPP). DPP expression in the large LNvs triggered a period lengthening phenotype, the downregulation of which caused reduced rhythmicity and affected anticipation at dawn and dusk, underscoring DPP per se conveys time-of-day relevant information. Surprisingly, DPP expression in the large LNvs impaired circadian remodeling of the small LNv axonal terminals, likely through local modulation of the guanine nucleotide exchange factor Trio. These findings open the provocative possibility that the BMP pathway is recruited to strengthen/reduce the connectivity among specific clusters along the day and thus modulate the contribution of the clusters to the circadian network. [ABSTRACT FROM AUTHOR]

Life is shaped by circadian clocks. This review focuses on how behavioral genetics in the fruit fly unveiled what is known today about circadian physiology. We will briefly summarize basic properties of the clock and focus on some clock-controlled behaviors to highlight how communication between central and peripheral oscillators defines their properties. [ABSTRACT FROM AUTHOR]

Background: Lipid homeostasis is an evolutionarily conserved process that is crucial for energy production, storage and consumption. Drosophila larvae feed continuously to achieve the roughly 200-fold increase in size and accumulate sufficient reserves to provide all energy and nutrients necessary for the development of the adult fly. The mechanisms controlling this metabolic program are poorly understood. Results: Herein we identified a highly conserved gene, orsai (osi), as a key player in lipid metabolism in Drosophila. Lack of osi function in the larval fat body, the regulatory hub of lipid homeostasis, reduces lipid reserves and energy output, evidenced by decreased ATP production and increased ROS levels. Metabolic defects due to reduced Orsai (Osi) in time trigger defective food-seeking behavior and lethality. Further, we demonstrate that downregulation of Lipase 3, a fat body-specific lipase involved in lipid catabolism in response to starvation, rescues the reduced lipid droplet size associated with defective orsai. Finally, we show that osi-related phenotypes are rescued through the expression of its human ortholog ETFRF1/LYRm5, known to modulate the entry of β-oxidation products into the electron transport chain; moreover, knocking down electron transport flavoproteins EtfQ0 and walrus/ETFA rescues osi-related phenotypes, further supporting this mode of action. Conclusions: These findings suggest that Osi may act in concert with the ETF complex to coordinate lipid homeostasis in the fat body in response to stage-specific demands, supporting cellular functions that in turn result in an adaptive behavioral response. [ABSTRACT FROM AUTHOR]

The bovine leukemia virus (BLV) causes leukemia or lymphoma in cattle. Although most BLV-infected animals do not develop the disease, they maintain the transmission chain of BLV at the herd level. As a feasible approach to control the virus, selection of cattle carrying the BoLA-DRB3*0902 allele has been proposed, as this allele is strongly associated with a BLV infection profile or the low proviral load (LPL) phenotype. To test whether these cattle affect the BLV transmission chain under natural conditions, selected BLV-infected LPL-BoLA-DRB3*0902 heterozygous cows were incorporated into a BLV-negative dairy herd. An average ratio of 5.4 (range 4.17-6.37) BLV-negative cows per BLV-infected cow was maintained during the 20 mo of the experiment, and no BLV-negative cattle became infected. The BLV incidence rate in this herd was thus zero, whereas BLV incidence rates in different local herds varied from 0.06 to 0.17 cases per 100 cattledays. This finding strongly suggests that LPL-BoLADRB3* 0902 cattle disrupted the BLV-transmission chain in the study period. [ABSTRACT FROM AUTHOR]

Reports on experiments into the circadian oscillator, which generates a rhythmic output with a period of about 24 hours, in which the Drosophilla CLOCK protein was shown to induce transcription of the circadian rhythm genes period and timeless.

The objective of this study was to estimate changes in body composition during the transition period of dairy cows with different feeding strategies during early lactation. Primiparous Holstein cows calved in autumn were used (n = 18; 528 ± 40 kg BW; 3.2 ± 0.2 BCS) in a randomized block design with two nutritional treatments. At calving and during the first 65 days postpartum (DPP), cows were assigned to either: (G0) total mixed ration (TMR) ad libitum (17kg DM/d offered; 70% forage, 30% concentrate) or (G1) grazing of alfalfa (Medicago sativa; 6-h am grazing in 3-d strips; pasture allowance=20 kg DM/d) + TMR (70% of ad libitum TMR; 12 kgDM/d offered). Cows were milked twice a day, milk yield was recorded daily, and all cows consumed 2.2 kgDM/day of a commercial concentrate at each milking. Cow BW and BCS were determined every two weeks from -21 to +60 DPP. At -7 and +42 ± 3 DPP, body composition was determined using the urea dilution technique. Data were analyzed as repeated measures with a mixed model including DPP and feeding strategy within DPP as fixed effects and block as random effect. Means were considered to differ when P < 0.05. Milk energy output during the first 42 DPP was greater (P = 0.05) for G0 than G1 cows (20.1 vs 18.3 ± 0.6 Mcal NEL/d) but loss of BCS from -7 to +42 DPP was greater (P = 0.04) for G1 than G0 cows. Cow body fat mass and retained gross energy (GE) decreased (P = 0.001) from pre to postpartum and these decreases were greater (P ≤ 0.20) for G1 than G0 cows (64.4503, and 59.4043 ± 2.5kg fat and 4007.65, and 4266.14 ± 129 MJ GE for -7 DPP and G0 and G1 at +42 DPP, respectively). In contrast, body protein and water mass were not affected by DPP or treatment. Relative to cow empty BW, body fat and GE content decreased (P ≤ 0.014) while body water and protein increased (P ≤ 0.04) from pre to postpartum. Although during the postpartum there were no differences in relative water and protein mass, relative body fat and GE content were less (P ≤ 0.001) for G1 than G0 cows (136.69 and 147.17± 5.2 kg fat and 9.2, and 9.7± 0.2 MJ GE for -7 DPP and G0 and G1 at +42 DPP, respectively). Negative energy balance during the transition period was more severe for G1 than G0 cows, probably associated to decreased DMI and increased maintenance requirements. [ABSTRACT FROM AUTHOR]

RalGPS2 is a Ras-independent Guanine Nucleotide Exchange Factor (GEF) for RalA containing a PH domain and an SH3-binding region and it is involved in several cellular processes, such as cytokinesis, control of cell cycle progression, differentiation, cytoskeleton organization and rearrangement. Up to now, few data have been published regarding RalGPS2 role in cancer cells, and its involvement in bladder cancer is yet to be established. In this paper we demonstrated that RalGPS2 is expressed in urothelial carcinoma-derived 5637 cancer cells and is essential for cellular growth. These cells produces thin membrane protrusions that displayed the characteristics of actin rich tunneling nanotubes (TNTs) and here we show that RalGPS2 is involved in the formation of these cellular protrusions. In fact the overexpression of RalGPS2 or of its PH-domain increased markedly the number and the length of nanotubes, while the knock-down of RalGPS2 caused a strong reduction of these structures. Moreover, using a series of RalA mutants impaired in the interaction with different downstream components (Sec5, Exo84, RalBP1) we demonstrated that the interaction of RalA with Sec5 is required for TNTs formation. Furthermore, we found that RalGPS2 interacts with the transmembrane MHC class III protein leukocyte specific transcript 1 (LST1) and RalA, leading to the formation of a complex which promotes TNTs generation. These findings allow us to add novel elements to molecular models that have been previously proposed regarding TNTs formation. [ABSTRACT FROM AUTHOR]

Circadian clocks organize the metabolism, physiology, and behavior of organisms throughout the day-night cycle by controlling daily rhythms in gene expression at the transcriptional and post-transcriptional levels. While many transcription factors underlying circadian oscillations are known, the splicing factors that modulate these rhythms remain largely unexplored. A genomewide assessment of the alterations of gene expression in a null mutant of the alternative splicing regulator SR-related matrix protein of 160 kDa (SRm160) revealed the extent to which alternative splicing impacts on behavior-related genes. We show that SRm160 affects gene expression in pacemaker neurons of the Drosophila brain to ensure proper oscillations of the molecular clock. A reduced level of SRm160 in adult pacemaker neurons impairs circadian rhythms in locomotor behavior, and this phenotype is caused, at least in part, by a marked reduction in period (per) levels. Moreover, rhythmic accumulation of the neuropeptide PIGMENT DISPERSING FACTOR in the dorsal projections of these neurons is abolished after SRm160 depletion. The lack of rhythmicity in SRm160-downregulated flies is reversed by a fully spliced per construct, but not by an extra copy of the endogenous locus, showing that SRm160 positively regulates per levels in a splicing-dependent manner. Our findings highlight the significant effect of alternative splicing on the nervous system and particularly on brain function in an in vivo model. [ABSTRACT FROM AUTHOR]

Genome biology approaches have made enormous contributions to our understanding of biological rhythms, particularly in identifying outputs of the clock, including RNAs, proteins, and metabolites, whose abundance oscillates throughout the day. These methods hold significant promise for future discovery, particularly when combined with computational modeling. However, genome-scale experiments are costly and laborious, yielding “big data” that are conceptually and statistically difficult to analyze. There is no obvious consensus regarding design or analysis. Here we discuss the relevant technical considerations to generate reproducible, statistically sound, and broadly useful genome-scale data. Rather than suggest a set of rigid rules, we aim to codify principles by which investigators, reviewers, and readers of the primary literature can evaluate the suitability of different experimental designs for measuring different aspects of biological rhythms. We introduce CircaInSilico, a web-based application for generating synthetic genome biology data to benchmark statistical methods for studying biological rhythms. Finally, we discuss several unmet analytical needs, including applications to clinical medicine, and suggest productive avenues to address them. [ABSTRACT FROM AUTHOR]

In the Drosophila brain, the neuropeptide PIGMENT DISPERSING FACTOR (PDF) is expressed in the small and large Lateral ventral neurons (LNvs) and regulates circadian locomotor behavior. Interestingly, PDF immunoreactivity at the dorsal terminals changes across the day as synaptic contacts do as a result of a remarkable remodeling of sLNv projections. Despite the relevance of this phenomenon to circuit plasticity and behavior, the underlying mechanisms remain poorly understood. In this work we provide evidence that PDF along with matrix metalloproteinases (Mmp1 and 2) are key in the control of circadian structural remodeling. Adult-specific downregulation of PDF levels per se hampers circadian axonal remodeling, as it does altering Mmp1 or Mmp2 levels within PDF neurons post-developmentally. However, only Mmp1 affects PDF immunoreactivity at the dorsal terminals and exerts a clear effect on overt behavior. In vitro analysis demonstrated that PDF is hydrolyzed by Mmp1, thereby suggesting that Mmp1 could directly terminate its biological activity. These data demonstrate that Mmp1 modulates PDF processing, which leads to daily structural remodeling and circadian behavior. [ABSTRACT FROM AUTHOR]

Intertidal species have both circadian and circatidal clocks. Although the behavioral evidence for these oscillators is more than 5 decades old, virtually nothing is known about their molecular clockwork. Pigment-dispersing hormones (PDHs) were originally described in crustaceans. Their insect homologs, pigment-dispersing factors (PDFs), have a prominent role as clock output and synchronizing signals released from clock neurons. We show that gene duplication in crabs has led to two PDH genes (β-pdh-I and β-pdh-II). Phylogenetically, β-pdh-I is more closely related to insect pdf than to β-pdh-II, and we hypothesized that β-PDH-I may represent a canonical clock output signal. Accordingly, β-PDH-I expression in the brain of the intertidal crab Cancer productus is similar to that of PDF in Drosophila melanogaster, and neurons that express PDH-I also show CYCLE-like immunoreactivity. Using D. melanogaster pdf-null mutants (pdf01) as a heterologous system, we show that β-pdh-I is indistinguishable from pdf in its ability to rescue the mutant arrhythmic phenotype, but β-pdh-II fails to restore the wild-type phenotype. Application of the three peptides to explanted brains shows that PDF and β-PDH-I are equally effective in inducing the signal transduction cascade of the PDF receptor, but β-PDH-II fails to induce a normal cascade. Our results represent the first functional characterization of a putative molecular clock output in an intertidal species and may provide a critical step towards the characterization of molecular components of biological clocks in intertidal organisms. [ABSTRACT FROM PUBLISHER]

Circadian rhythms allow organisms to time biological processes to the most appropriate phases of the day-night cycle. Post-transcriptional regulation is emerging as an important component of circadian networks, but the molecular mechanisms linking the circadian clock to the control of RNA processing are largely unknown. Here we show that PROTEIN ARGININE METHYL TRANSFERASE 5 (PRMT5), which transfers methyl groups to arginine residues present in histones and Sm spliceosomal proteins, links the circadian clock to the control of alternative splicing in plants. Mutations in PRMT5 impair several circadian rhythms in Arabidopsis thaliana and this phenotype is caused, at least in part, by a strong alteration in alternative splicing of the core-clock gene PSEUDO RESPONSE REGULATOR 9 (PRR9). Furthermore, genome-wide studies show that PRMT5 contributes to the regulation of many pre-messenger-RNA splicing events, probably by modulating 5′-splice-site recognition. PRMT5 expression shows daily and circadian oscillations, and this contributes to the mediation of the circadian regulation of expression and alternative splicing of a subset of genes. Circadian rhythms in locomotor activity are also disrupted in dart5-1, a mutant affected in the Drosophila melanogaster PRMT5 homologue, and this is associated with alterations in splicing of the core-clock gene period and several clock-associated genes. Our results demonstrate a key role for PRMT5 in the regulation of alternative splicing and indicate that the interplay between the circadian clock and the regulation of alternative splicing by PRMT5 constitutes a common mechanism that helps organisms to synchronize physiological processes with daily changes in environmental conditions. [ABSTRACT FROM AUTHOR]

Abstract: Neurodegenerative diseases encompass a broad variety of motor and cognitive disorders that are accompanied by death of specific neuronal populations or brain regions. Cellular and molecular mechanisms underlying these complex disorders remain largely unknown. In a previous work we searched for novel Drosophila genes relevant for neurodegeneration and singled out enabled (ena), which encodes a protein involved in cytoskeleton remodeling. To extend our understanding on the mechanisms of ENA-triggered degeneration we now investigated the effect of silencing ena ortholog genes in mouse hippocampal neurons. We found that ENA/VASP downregulation led to neurite retraction and concomitant neuronal cell death through an apoptotic pathway. Remarkably, this retraction initially affected the axonal structure, showing no effect on dendrites. Reduction in ENA/VASP levels blocked the neuritogenic effect of a specific RhoA kinase (ROCK) inhibitor, thus suggesting that these proteins could participate in the Rho-signaling pathway. Altogether these observations demonstrate that ENA/VASP proteins are implicated in the establishment and maintenance of the axonal structure and that a change on their expression levels triggers neuronal degeneration. [Copyright &y& Elsevier]

The synaptic membrane-associated guanylate kinase (MAGUK) scaffolding protein family is thought to play key roles in synapse assembly and synaptic plasticity. Evidence supporting these roles in vivo is scarce, as a consequence of gene redundancy in mammals. The genome of Drosophila contains only one MAGUK gene, discs large (dlg), from which two major proteins originate:DLGA[PSD95 (postsynaptic density 95)-like] and DLGS97 [SAP97 (synapse-associated protein)-like]. These differ only by the inclusion in DLGS97 of an L27 domain, important for the formation of supramolecular assemblies. Known dlg mutations affect both forms and are lethal at larval stages attributable to tumoral overgrowth of epithelia. We generated independent null mutations for each, dlgA and dlgS97. These allowed unveiling of a shift in expression during the development of the nervous system: predominant expression of DLGA in the embryo, balanced expression of both during larval stages, and almost exclusive DLGS97 expression in the adult brain. Loss of embryonic DLGS97 does not alter the development of the nervous system. At larval stages, DLGA and DLGS97 fulfill both unique and partially redundant functions in the neuromuscular junction. Contrary to dlg and dlgA mutants, dlgS97 mutants are viable to adulthood, but they exhibit marked alterations in complex behaviors such as phototaxis, circadian activity, and courtship, whereas simpler behaviors like locomotion and odor and light perception are spared. We propose that the increased repertoire of associations of a synaptic scaffold protein given by an additional domain of protein-protein interaction underlies its ability to integrate molecular networks required for complex functions in adult synapses. [ABSTRACT FROM AUTHOR]

Abstract: In the Oltrepo Pavese (Northern Italy), several areas subjected to risk assessment according to Italian law are located around a highly fractured sandstone slabs (M. Vallassa Sandstone) lying on a deformable clayey substratum (Epiligurian Sequence). The structural and geomorphologic surveys and comparison with results obtained by some authors in similar geological contexts indicate the possible presence of deep-seated gravitational deformations, which could be classified as lateral spread. The potentiality of the Permanent Scatterers technique has been explored by the authors for investigating large slope displacements in the Oltrepo Pavese. The Permanent Scatterers analysis was combined with in situ conventional geomorphological, structural and geotechnical studies. Test sites, corresponding to sandstone slabs and areas classified at high risk from the institutional authorities, were selected. A geological model of each test area was obtained from the integration of the geological–geomorphological character of the site with the geotechnical data (boreholes, piezometers and laboratory tests). The PS technique allows to detect and delimit the unstable area and to identify some zones within the mass movement characterized by different displacements rates, and thus different degree of hazard. [Copyright &y& Elsevier]

Summary: Previous work in Drosophila has defined two populations of circadian brain neurons, morning cells (M-cells) and evening cells (E-cells), both of which keep circadian time and regulate morning and evening activity, respectively. It has long been speculated that a multiple oscillator circadian network in animals underlies the behavioral and physiological pattern variability caused by seasonal fluctuations of photoperiod. We have manipulated separately the circadian photoentrainment pathway within E- and M-cells and show that E-cells process light information and function as master clocks in the presence of light. M-cells in contrast need darkness to cycle autonomously and dominate the network. The results indicate that the network switches control between these two centers as a function of photoperiod. Together with the different entraining properties of the two clock centers, the results suggest that the functional organization of the network underlies the behavioral adjustment to variations in daylength and season. [Copyright &y& Elsevier]

Abstract: The applicability of the Permanent Scatterers Synthetic Aperture Radar Interferometry (PSInSAR) technique for detecting and monitoring ground displacements was tested in the Oltrepo Pavese territory (Northern Italy, southern Lombardia), which could be representative of similar geological contexts in the Italian Apennines. The study area, which extends for almost 1100 km2, is characterized by a complex geological and structural setting and the presence of clay-rich sedimentary formations. These characteristics make the Oltrepo Pavese particularly prone to several geological hazards: shallow and deep landslides, subsidence and swelling/shrinkage of the clayey soils. The PSInSAR technique used in this study overcomes most of the limitations of conventional interferometric approaches by identifying, within the area of interest, a set of “radar benchmarks” (PS), where very precise displacement measurements can be carried out. More than 90,000 PS were identified by processing Synthetic Aperture Radar (SAR) images acquired from 1992 to 2001 by the European Remote Sensing satellites (ERS). The PSInSAR application at a sub-regional scale detected slow ground deformations ranging from +5 to −16 mm/year, and resulting from various processes (landslides, swelling/shrinkage of clay soils and water pumping). The PS displacements were analysed by collecting data obtained through geological, geomorphologic field surveys, geotechnical analysis of the soils and the information was integrated within a landslide inventory and the damaged building inventory. Despite the limited number of landslide bodies with PS (7% of the inventoried landslides), the PS data helped to revise the state of activity of several landslides. Furthermore, some previously unknown unstable slopes were detected. Two areas of uplift and two areas of subsidence were identified. [Copyright &y& Elsevier]

The seasonal variation of PM1 and PM2.5 mass concentration and composition has been investigated in the great urban area of Milan. PM samplings were carried out 3 times a week during the year 2002. Mass and elemental concentrations were measured in all samples and in a limited number of filters also the elemental and organic carbon contribution (EC and OC) was determined.In the urban area of Milan, PM2.5 comprises about 60% and 70% of the PM1 mass in summer and in winter, respectively. Among the measured PM components carbon compounds and sulphates are by far the major contributors and a significant contribution likely due to nitrates (not measured) should also be considered. PM1 and PM2.5 concentration and composition show a strong seasonal behaviour, which was studied in relation to atmospheric dispersion conditions.Our approach is based on the use of Radon concentration as a natural tracer of differences in the average mixing layer height during the year. The application of a suitable “Radon index” allows the normalisation of PM mass and elemental data to account for the seasonal effect due to differences in atmospheric dispersion conditions. [Copyright &y& Elsevier]

Behavioral outputs arise as a result of highly regulated yet flexible communication among neurons. The Drosophila circadian network includes 150 neurons that dictate the temporal organization of locomotor activity; under light-dark (LD) conditions, flies display a robust bimodal pattern. The pigment-dispersing factor (PDF)-positive small ventral lateral neurons (sLNv) have been linked to the generation of the morning activity peak (the "M cells"), whereas the Cryptochrome (CRY)-positive dorsal lateral neurons (LNds) and the PDF-negative sLNv are necessary for the evening activity peak (the "E cells") [ 1 , 2 ]. While each group directly controls locomotor output pathways [ 3 ], an interplay between them along with a third dorsal cluster (the DN1ps) is necessary for the correct timing of each peak and for adjusting behavior to changes in the environment [ 4–7 ]. M cells set the phase of roughly half of the circadian neurons (including the E cells) through PDF [ 5 , 8–10 ]. Here, we show the existence of synaptic input provided by the evening oscillator onto the M cells. Both structural and functional approaches revealed that E-to-M cell connectivity changes across the day, with higher excitatory input taking place before the day-to-night transition. We identified two different neurotransmitters, acetylcholine and glutamate, released by E cells that are relevant for robust circadian output. Indeed, we show that acetylcholine is responsible for the excitatory input from E cells to M cells, which show preferential responsiveness to acetylcholine during the evening. Our findings provide evidence of an excitatory feedback between circadian clusters and unveil an important plastic remodeling of the E cells' synaptic connections. • E cells exhibit structural remodeling of their presynaptic terminals across the day • E cells release two neurotransmitters, acetylcholine and glutamate • Activation of E cells depolarizes M cells preferentially during late day • Hindering neurotransmission in E cells impairs clock outputs In nature, Drosophila organizes its activity around dawn and dusk, relying on its clock. Clock neurons undergo structural remodeling, and hence, their synaptic connections change daily. Duhart et al. demonstrate that circadian remodeling fine-tunes the communication between clock clusters and thus modulates their output to the circadian network. [ABSTRACT FROM AUTHOR]