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Distinguishing renal oncocytoma (RO) from the eosinophilic variant of chromophobe renal cell carcinoma (ChRCC) under the light microscope is a common diagnostic problem. Our recent research has shown significant difference between the presence of tumor fibrous capsule in ChRCCs and ROs. Transforming growth factor beta 1 (TGF-β1) is a potent cytokine involved in regulating a number of cellular processes. Two main purposes of this research were to investigate whether the TGF-β1 staining could be related to the presence of tumor fibrous capsule and if it could be used in the differential diagnosis between ChRCC and RO. We investigated 34 cases: 16 ChRCCs (8 eosinophilic and 8 classic) and 18 ROs. All available slides of each tumor, routinely stained with hematoxylin and eosin (H&E) were first analyzed to note the presence of tumor fibrous capsule. One paraffin embedded tissue block matching the representative H&E slide was selected for the immunohistochemical analysis. TGF-β1 expression was analyzed semiquantitatively in the tumor tissue, the tumor fibrous capsule, if present and the peritumoral renal parenchyma. Intensity of TGF-β1 expression was weaker in ChRCCs than the one observed in ROs (P

Distinguishing renal oncocytoma (RO) from the eosinophilic variant of chromophobe renal cell carcinoma (ChRCC) under the light microscope is a common diagnostic problem. Our recent research has shown significant difference between the presence of tumor fibrous capsule in ChRCCs and ROs. Transforming growth factor beta 1 (TGF-β1) is a potent cytokine involved in regulating a number of cellular processes. Two main purposes of this research were to investigate whether the TGF- β1 staining could be related to the presence of tumor fibrous capsule and if it could be used in the differential diagnosis between ChRCC and RO. We investigated 34 cases: 16 ChRCCs (8 eosinophilic and 8 classic) and 18 ROs. All available slides of each tumor, routinely stained with hematoxylin and eosin (H&E) were first analyzed to note the presence of tumor fibrous capsule. One paraffin embedded tissue block matching the representative H&E slide was selected for the immunohistochemical analysis. TGF-β1 expression was analyzed semiquantitatively in the tumor tissue, the tumor fibrous capsule, if present and the peritumoral renal parenchyma. Intensity of TGF-β1 expression was weaker in ChRCCs than the one observed in ROs (P

Perirenal involvement in lymphoma usually occurs due to contiguous spread from retroperitoneal or renal lymphoma. The occurrence of isolated lymphoma in the perirenal space is very unusual (

The most important and challenging differential diagnosis of chromophobe renal cell carcinoma (CRCC) is renal oncocytoma (RO).Recent search has shown differences in the presence and thickness of tumor surrounding capsule between CRCC and RO.

Introduction Renal oncocytoma is a benign epithelial tumor, composed of oncocytes, large eosinophilic cells. It represents 3-9% of renal tumors. Chromophobe renal cell carcinoma (ChRCC) is a distinct subtype of renal cell carcinoma and comprises approximately 5% of all renal tumors. Two main variants are classic and eosinophilic ChRCC. Although considered less aggressive than other renal cell carcinomas, ChRCC has a metastatic potential. Due to overlapping morphological characteristics of renal oncocytoma and ChRCC, particularly its eosinophilic variant with the abundant granular eosinophilic cytoplasm, making a correct diagnosis can be challenging. Aim The aim of this study was to assess and compare the apoptotic index, defined as the absolute number of apoptotic cells per 10 high power fields (HPFs), in the two tumor groups. Materials and methods The files from Ljudevit Jurak Univesity Department of Pathology, Sestre milosrdnice University Hospital were searched for cases of histologically confirmed ChRCCs and renal oncocytomas. Slides of 10 ChRCCs and 10 renal oncocytomas, stained by haematoxylin and eosin were analyzed under the light microscope. Apoptotic cell was defined as a cell with dense, eosinophilic cytoplasm and picnotic or defragmented nucleus. The absolute number of apoptotic cells per 10 HPFs in the two tumor groups was counted. Results In the group of renal oncocytomas there were average 7.8 % apoptotic cells per 10 HPFs (range 4-12). In the group of ChRCCs there were 2.5% apoptotic cells per 10 HPFs (range 1-6). Discussion Apoptosis is the process of programmed cell death. Dysregulation of apoptosis is a key process in cancer development and progression. The aim of this study was to assess and compare the apoptotic index in the two tumor groups. Altough there is a visible difference in the average number of apoptotic cells between the two tumor groups, this study is partially limited by the modest number of cases and further studies are needed to explore the possible use of assessing the apoptotic index in the differential diagnosis between renal oncocytoma and ChRCC.

The abstract describes differences in the presence of capsule around chromophobe renal cell carcinoma and oncocytoma. It also describes differences in capsule diameter.