Search

Your search keyword '"Chéreau, Sylvain"' showing total 39 results
Start Over Author "Chéreau, Sylvain"
39 results on '"Chéreau, Sylvain"'

3. Workflow4Metabolomics (W4M): a user-friendly metabolomics platform for analysis of MS and NMR data

Author

Delporte, Cédric, Tremblay-Franco, Marie, Guitton, Y., Canlet, Cecile CC, Weber, Ralf J. M., Hecht, Helge, Price, Elliott James, Klanova, Jana, Joly, Charlotte, Dalle, Céline, Saint-Vanne, Julien, Thévenot, Etienne E.A., Schmitz, Isabelle, Chéreau, Sylvain, Dechaumet, Sylvain, Diémé, Binta, Giacomoni, Franck FG, Le Corguillé, Gildas G C, Pétéra, Mélanie MP, Souard, Florence, Delporte, Cédric, Tremblay-Franco, Marie, Guitton, Y., Canlet, Cecile CC, Weber, Ralf J. M., Hecht, Helge, Price, Elliott James, Klanova, Jana, Joly, Charlotte, Dalle, Céline, Saint-Vanne, Julien, Thévenot, Etienne E.A., Schmitz, Isabelle, Chéreau, Sylvain, Dechaumet, Sylvain, Diémé, Binta, Giacomoni, Franck FG, Le Corguillé, Gildas G C, Pétéra, Mélanie MP, and Souard, Florence

Abstract

info:eu-repo/semantics/inPress

Published
2024

12. Natural occurrence of mycotoxin-producing fusaria in market-bought peruvian cereals

Author

Ducos, Christine, Pinson-Gadais, Laetitia, Chéreau, Sylvain, Richard-Forget, Florence, Vásquez-Ocmín, Pedro, Cerapio, Juan Pablo, Casavilca-Zambrano, Sandro, Ruiz, Eloy, Pineau, Pascal, Bertani, Stephane, Ponts, Nadia, Unité de recherche Mycologie et Sécurité des Aliments (MycSA), Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Pharmacochimie et Biologie pour le Développement (PHARMA-DEV), Institut de Recherche pour le Développement (IRD)-Institut de Chimie de Toulouse (ICT), Institut de Recherche pour le Développement (IRD)-Université Toulouse III - Paul Sabatier (UT3), Université de Toulouse (UT)-Université de Toulouse (UT)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Institut National Polytechnique (Toulouse) (Toulouse INP), Université de Toulouse (UT)-Institut de Recherche pour le Développement (IRD)-Université Toulouse III - Paul Sabatier (UT3), Université de Toulouse (UT), Organisation Nucléaire et Oncogenèse / Nuclear Organization and Oncogenesis, Institut Pasteur [Paris] (IP)-Institut National de la Santé et de la Recherche Médicale (INSERM), Instituto Nacional de Enfermedades Neoplasicas [Lima, Pérou] (INEN), This research was funded by the French National Alliance for Life Sciences and Health, grant number ENV201408. S.B. has received funding from the European Union’s Horizon 2020 Framework program under the Marie Skłodowska-Curie Actions, agreement number 823935. P.V.O. was supported by a postdoctoral fellowship from the French National Research Institute for Sustainable Development (IRD) (04077858). JPC was a recipient of a doctoral fellowship from the Peruvian National Council for Science and Technology (212-2015-FONDECYT)., and European Project: 823935,H2020-MSCA-RISE-2018,COCLICAN(2018)

Subjects
MESH: Fusarium, MESH: Humans, MESH: Dietary Exposure, MESH: Food Supply, MESH: Zea mays, [SDV]Life Sciences [q-bio], MESH: Consumer Product Safety, MESH: Mycotoxins, MESH: Risk Assessment, MESH: Commerce, corn, [SDV.BV.AP]Life Sciences [q-bio]/Vegetal Biology/Plant breeding, MESH: Edible Grain, Fumonisins B, Peru, [SDE]Environmental Sciences, [SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology, MESH: Food Microbiology, MESH: Peru, [SDV.AEN]Life Sciences [q-bio]/Food and Nutrition
Abstract

International audience; Consumption of cereals contaminated by mycotoxins poses health risks. For instance, Fumonisins B, mainly produced by Fusarium verticillioides and Fusarium proliferatum, and the type B trichothecene deoxynivalenol, typically produced by Fusarium graminearum, are highly prevalent on cereal grains that are staples of many cultural diets and known to represent a toxic risk hazard. In Peru, corn and other cereals are frequently consumed on a daily basis under various forms, the majority of food grains being sold through traditional markets for direct consumption. Here, we surveyed mycotoxin contents of market-bought grain samples in order to assess the threat these mycotoxins might represent to Peruvian population, with a focus on corn. We found that nearly one sample of Peruvian corn out of six was contaminated with very high levels of Fumonisins, levels mostly ascribed to the presence of F. verticillioides. Extensive profiling of Peruvian corn kernels for fungal contaminants could provide elements to refine the potential risk associated with Fusarium toxins and help define adapted food safety standards.

Published
2021
Full Text
View/download PDF

13. Efficiency of Hydroxycinnamic Phenolic Acids to Inhibit the Production of Ochratoxin A by Aspergillus westerdijkiae and Penicillium verrucosum

Author

Boonmee, Saranyaphat, Atanasova, Vessela, Chéreau, Sylvain, Marchegay, Gisèle, Hyde, Kevin, Richard-Forget, F., Mae Fah Luang University [Thaïlande] (MFU), Unité de recherche Mycologie et Sécurité des Aliments (MycSA), and Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)

Subjects
Coumaric Acids, [SDV]Life Sciences [q-bio], Penicillium, Penicillium verrucosum, Aspergillus westerdijkiae, Ochratoxins, Article, lcsh:Chemistry, Aspergillus, lcsh:Biology (General), lcsh:QD1-999, phenolic acids, lcsh:QH301-705.5, ochratoxin A
Abstract

Ochratoxin A (OTA) is one of the worldwide most important mycotoxins in terms of health and agroeconomic consequences. With the aim to promote the use of phytochemicals as alternatives to synthetic fungicides, the effect of hydroxycinnamic acids on the fungal growth and OTA yield by two major OTA-producing species was investigated. After a first step dedicated to the definition of most suitable culture conditions, the impact of 0.5 mM ferulic (FER), p-coumaric (COUM), caffeic and chlorogenic acids was evaluated on Aspergillus westerdijkiae and Penicillium verrucosum. Whereas no fungal growth reduction was observed regardless of the phenolic acid and fungal isolate, our results demonstrated the capacity of FER and COUM to inhibit OTA production. The most efficient compound was FER that led to a 70% reduction of OTA yielded by P. verrucosum and, although not statistically significant, a 35% inhibition of OTA produced by A. westerdijkiae. To further investigate the bioactivity of FER and COUM, their metabolic fate was characterized in fungal broths. The capacity of P. verrucosum to metabolize FER and COUM through a C2-clivage type degradation was demonstrated. Overall, our data support the potential use of FER to prevent OTA contamination and reduce the use of synthetic pesticides.

Published
2020
Full Text
View/download PDF

14. Investigating the Efficiency of Hydroxycinnamic Acids to Inhibit the Production of Enniatins by Fusarium avenaceum and Modulate the Expression of Enniatins Biosynthetic Genes

Author

Gautier, Charlotte, primary, Pinson-Gadais, Laetitia, additional, Verdal-Bonnin, Marie-Noelle, additional, Ducos, Christine, additional, Tremblay, Judith, additional, Chéreau, Sylvain, additional, Atanasova, Vessela, additional, and Richard-Forget, Florence, additional

Published
2020
Full Text
View/download PDF

16. Priming for protecting maize seedlings from accumulation of fumonisins in early stages of infection by the mycotoxinogen fungus, Fusarium verticillioides

Author

Savoie, Jean-Michel, Chéreau, Sylvain, Ferrer, Nathalie, Ducos, Christine, Pinson-Gadais, Laetitia, Richard-Forget, Florence, Unité de recherche Mycologie et Sécurité des Aliments (MycSA), Institut National de la Recherche Agronomique (INRA), and Institut National de Recherche Agronomique (INRA). UR Mycologie et Sécurité des Aliments (1264).

Subjects
[SDV]Life Sciences [q-bio], [SDE]Environmental Sciences, ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2018

17. Contamination des farines céréalières des marchés d’Abidjan : mycoflore et mycotoxines

Author

Koffi, Marc K., Chéreau, Sylvain, Pinson-Gadais, Laetitia, Ferrer, Nathalie, Atanasova-Penichon, Vessela, Savoie, Jean-Michel, Richard-Forget, Florence, Unité de recherche Mycologie et Sécurité des Aliments (MycSA), Institut National de la Recherche Agronomique (INRA), and Institut National de Recherche Agronomique (INRA). UR Mycologie et Sécurité des Aliments (1264).

Subjects
[SDV]Life Sciences [q-bio], [SDE]Environmental Sciences, ComputingMilieux_MISCELLANEOUS
Abstract

National audience

Published
2018

18. Limiter la contamination du blé dur par le déoxynivalénol : Caractérisation de nouvelles sources de résistance à la fusariose

Author

Chéreau, Sylvain, Atanasova-Penichon, Vessela, Hadjout, Salah, Lacou, Julie, Roumet, Pierre, Forget, Florence, Unité de recherche Mycologie et Sécurité des Aliments (MycSA), Institut National de la Recherche Agronomique (INRA), Amélioration génétique et adaptation des plantes méditerranéennes et tropicales (UMR AGAP), Institut National de la Recherche Agronomique (INRA)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad), and Institut National de Recherche Agronomique (INRA). UR Mycologie et Sécurité des Aliments (1264).

Subjects
[SDV]Life Sciences [q-bio], [SDE]Environmental Sciences, [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, ComputingMilieux_MISCELLANEOUS
Abstract

National audience

Published
2018

19. Mycotoxin biosynthesis and central metabolism are two interlinked pathways in Fusarium graminearum, as demonstrated by the extensive metabolic changes induced by caffeic acid exposure

Author

Chéreau, Sylvain, Atanasova-Penichon, Vessela, Legoahec, Laurie, Bernillon, Stéphane, Deborde, Catherine, Maucourt, Mickael, Verdal-Bonnin, Marie-Noëlle, Pinson-Gadais, Laetitia, Moing, Annick, Ponts, Nadia, Richard-Forget, Florence, Unité de recherche Mycologie et Sécurité des Aliments (MycSA), Institut National de la Recherche Agronomique (INRA), Unité de recherche sur les Biopolymères, Interactions Assemblages (BIA), Biologie du fruit et pathologie (BFP), Université Bordeaux Segalen - Bordeaux 2-Institut National de la Recherche Agronomique (INRA)-Université Sciences et Technologies - Bordeaux 1, and Universität für Bodenkultur Wien [Vienne, Autriche] (BOKU). AUT.

Subjects
[SDV]Life Sciences [q-bio], [SDE]Environmental Sciences, [SDV.IDA]Life Sciences [q-bio]/Food engineering, [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, [SPI.GPROC]Engineering Sciences [physics]/Chemical and Process Engineering, ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2018

20. Phenotypic and biochemical characterization of new advanced durum wheat breeding lines from Algeria that show resistance to fusarium head blight and to mycotoxin accumulation

Author

Hadjout, S., Chéreau, Sylvain, Atanasova-Penichon, Vessela, Marchegay, Gisele, Mekliche, Leila, Boureghda, Houda, Barreau, Christian, Touati-Hattab, Sihem, Bouznad, Zouazi, Richard-Forget, Florence, École Nationale Supérieure d’Agronomie [Alger] (ENSA), Unité de recherche Mycologie et Sécurité des Aliments (MycSA), and Institut National de la Recherche Agronomique (INRA)

Subjects
p-coumaric acid, phenolic acid, [SDV]Life Sciences [q-bio], [SDE]Environmental Sciences, trichothecenes, food and beverages, durum wheat, Fusarium culmorum
Abstract

International audience; Durum wheat (Triticum turgidum L. var. durum) is a crop highly susceptible to Fusarium head blight (FHB), which results in yield losses and downgrades the quality of grains mostly due to mycotoxin contamination. Although breeding for resistance to FHB is one of the most promising strategy for minimizing crop damage, the attempts to obtain durum wheat resistant lines have been limited so far. Two potentially interesting lines were recently delivered by a breeding program based on crosses involving cultivars from Europe and Syria. Using a field experiment in Algeria with four different Fusarium culmorum strains and a spray inoculation method, we demonstrated that the two breeding lines were significantly less affected than a set of commercial cultivars. The two breeding lines were shown to exhibit a higher resistance to both initial fungal infection and disease spread , and to mycotoxin contamination. In addition, a detailed analysis of 2 phenolic acid composition of grains indicates significant differences in p-coumaric acid between the FHB-resistant breeding lines and the sensitive genotypes. Our results corroborate the importance of cell wall composition in preventing the diffusion of F. culmorum and therefore contributing to the resistance of cereals to FHB.

Published
2017
Full Text
View/download PDF

22. Corn grain ensiling is a process conducive to fumonisin B1 degradation: which microbial consortia are responsible ?

Author

Martinez-Tuppia, Ccori, Atanasova-Penichon, Vessela, Chéreau, Sylvain, Ferrer, Nathalie, Marchegay, Gisele, Castex, M., Ponts, Nadia, Theil, Sébastien, Savoie, Jean-Michel, Richard-Forget, Florence, Unité de recherche Mycologie et Sécurité des Aliments (MycSA), Institut National de la Recherche Agronomique (INRA), Lallemand S.A.S., and Unité Mixte de Recherche Fromagère (UMRF)

Subjects
[SDV]Life Sciences [q-bio], [SDE]Environmental Sciences, ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2016

23. High moisture maize grain ensiling is a process conductive to fumonisin B1 biodegradation

Author

Martinez-Tuppia, Ccori, Theil, Sébastien, Ponts, Nadia, Atanasova-Penichon, Vessela, Ferrer, Nathalie, Chéreau, Sylvain, Richard-Forget, Florence, Savoie, Jean-Michel, DROUIN, P., Unité de recherche Mycologie et Sécurité des Aliments (MycSA), Institut National de la Recherche Agronomique (INRA), Unité Mixte de Recherche Fromagère (UMRF), and Lallemand S.A.S.

Subjects
[SDV]Life Sciences [q-bio], [SDE]Environmental Sciences, ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2016

24. Biotraitement des grains pour amoindrir le risque mycotoxique: cas de la fumonisine B1 dans l’ensilage de maïs grain humide

Author

Martinez-Tuppia, Ccori, Ferrer, Nathalie, Theil, Sébastien, Atanasova-Penichon, Vessela, Chéreau, Sylvain, Ponts, Nadia, Richard-Forget, Florence, Savoie, Jean-Michel, Unité de recherche Mycologie et Sécurité des Aliments (MycSA), Institut National de la Recherche Agronomique (INRA), Unité Mixte de Recherche Fromagère (UMRF), and Institut National de Recherche Agronomique (INRA). UR Mycologie et Sécurité des Aliments (1264).

Subjects
[SDV]Life Sciences [q-bio], [SDE]Environmental Sciences, ComputingMilieux_MISCELLANEOUS
Abstract

National audience

Published
2016

26. Méthodologie de criblage rapide in vitro de l’activité antifongique de substances naturelles bioactives sur cultures Pathogénicité, production de trichotécènes par Fusarium culmorum et évaluation de la résistance des variétés de blé cultivées en Algérie

Author

Touati-Hattab, Sihem, Barreau, Christian, Verdal-Bonnin, Marie-Noëlle, Chéreau, Sylvain, Richard-Forget, Florence, Hadjout, Salah, Mekliche, Leila, Bouznad, Zouaoui, Université Amar Telidji - Laghouat, Unité de recherche Mycologie et Sécurité des Aliments (MycSA), Institut National de la Recherche Agronomique (INRA), and École Nationale Supérieure d’Agronomie [Alger] (ENSA)

Subjects
[SDV]Life Sciences [q-bio], [SDE]Environmental Sciences, ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2015

27. Application of metabolomics to the biochemical phenotyping of resistant maize lines to Fusarium graminearum

Author

Gauthier, Léa, Atanasova-Penichon, Vessela, Bernillon, Stéphane, Chéreau, Sylvain, Maucourt, Mickael, Moing, Annick, Mey, G., Surault, A., Roussel, M., Chopin, F., Fouquet, R., Richard-Forget, Florence, Unité de recherche Mycologie et Sécurité des Aliments (MycSA), Institut National de la Recherche Agronomique (INRA), Biologie du fruit et pathologie (BFP), Université Bordeaux Segalen - Bordeaux 2-Institut National de la Recherche Agronomique (INRA)-Université Sciences et Technologies - Bordeaux 1, Euralis Semences, Monsanto Company, Réseau Francophone de Métabolomique et Fluxomique (RFMF). FRA., and ProdInra, Migration

Subjects
[SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology, [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, [SDV.BV] Life Sciences [q-bio]/Vegetal Biology, [SDV.MP.MYC] Life Sciences [q-bio]/Microbiology and Parasitology/Mycology, [SDV.MP] Life Sciences [q-bio]/Microbiology and Parasitology, ComputingMilieux_MISCELLANEOUS, [SDV.MP.MYC]Life Sciences [q-bio]/Microbiology and Parasitology/Mycology
Abstract

National audience

Published
2015

28. Priming to protect maize from Fusarium verticillioides and its fumonisin accumulation.

Author

Aguado, Ana, Savoie, Jean‐Michel, Chéreau, Sylvain, Ducos, Christine, Aguilar, María, Ferrer, Nathalie, Aguilar, Manuel, Pinson‐Gadais, Laetitia, and Richard‐Forget, Florence

Subjects
CORN, GERMINATION, FUMONISINS, PLANT protection, SEEDS
Abstract

BACKGROUND Systemic infection through the seed is one of the routes used by the mycotoxinogenic pathogen Fusarium verticillioides for colonizing maize plants. The prohibition of the use of most chemical fungicides by the EU has promoted research on plant resistance inducers as an effective and sustainable alternative. Induction of a priming state in maize seeds might affect their susceptibility to contamination and accumulation of fumonisins. This state by application of a natural fertilizer called Chamae on maize seeds, was investigated in two varieties to control the colonization by the fungus and the accumulation of fumonisins B1, B2 and B3, germinating seeds, dead plants and yield. RESULTS: After inoculation of F. verticillioides on germinating seeds, the colonization by the fungus and the accumulation of fumonisins were significantly lower in seedlings coming from treated seeds, but a significant number of plants stopped their development by necrosis. In a field trial, the 0.01% (v/v) application dilution showed a lower plant density, although the level of biomass at harvest was not affected. CONCLUSION: The priming state contributed to the control of F. verticillioides development from seed infection and fumonisin accumulation in the early stage of plant growth, without affecting the final crop yield, and could reduce fungicide use and environmental contamination. © 2018 Society of Chemical Industry [ABSTRACT FROM AUTHOR]

Published
2019
Full Text
View/download PDF

31. Metabolomics in food analysis: application to the control of forbidden substances

Author

Dervilly-Pinel, Gaud, Courant, Frederique, Chéreau, Sylvain, Royer, Anne-Lise, Boyard-Kieken, Fanny, Antignac, Jean-Philippe, Monteau, Fabrice, Le Bizec, Bruno, Laboratoire d'étude des Résidus et Contaminants dans les Aliments (LABERCA), Institut National de la Recherche Agronomique (INRA)-Ecole Nationale Vétérinaire, Agroalimentaire et de l'alimentation Nantes-Atlantique (ONIRIS), Unité de recherche Mycologie et Sécurité des Aliments (MSA), Institut National de la Recherche Agronomique (INRA), Laboratoire des Courses Hippiques (LCH), and Unité de recherche Mycologie et Sécurité des Aliments (MycSA)

Subjects
EXTRACTION, STRATEGIES, screening, BIOMARKERS, ss-agonists, CATTLE, METABONOMICS, ANABOLIC-STEROIDS, metabolomics, METABOLITES, CALVES URINE, untargeted profiling, growth promoters, [CHIM.ANAL]Chemical Sciences/Analytical chemistry, growth hormone, BIOLOGICAL SAMPLES, [SDV.AEN]Life Sciences [q-bio]/Food and Nutrition, CHROMATOGRAPHY-MASS SPECTROMETRY, ComputingMilieux_MISCELLANEOUS, steroids
Abstract

International audience; Metabolomics is a science of interest in food analysis to describe and predict properties of food products and processes. It includes the development of analytical methods with the ultimate goal being the identification of so-called quality markers, (i.e. sets of metabolites that correlate with, for example, quality, safety, taste, or fragrance of foodstuffs). In turn, these metabolites are influenced by factors as genetic differences of the raw food ingredients (such as animal breed or crop species differences), growth conditions (such as climate, irrigation strategy, or feeding) or production conditions (such as temperature, acidity, or pressure). In cases where the routine-based measurement of a food property faces some limitations such as the lack of knowledge regarding the target compounds to monitor, monitoring based on a limited set of crucial biomarkers is a good alternative, which is of great interest for food safety purposes regarding growth promoting practices. Such an approach may be more efficient than using a classic approach based on a limited set of known metabolites of anabolic compounds. In this context, screening strategies allowing detection of the physiological response resulting from anabolic compound administration are promising approaches to detect their misuse. The global metabolomics workflow implemented for such studies is presented and illustrated through various examples of biological matrices profiling (tissue, blood, urine) and for different classes of anabolic compounds (steroids, beta-agonists and somatotropin). (c) 2012 John Wiley & Sons, Ltd.

Published
2012
Full Text
View/download PDF

33. Yeast and bacteria from ensiled high moisture maize grains as potential mitigation agents of fumonisin B1.

Author

Martinez Tuppia, Ccori, Atanasova‐Penichon, Vessela, Chéreau, Sylvain, Ferrer, Nathalie, Marchegay, Gisèle, Savoie, Jean‐Michel, and Richard‐Forget, Florence

Subjects
BIODEGRADATION, MYCOTOXINS, FUMONISINS, SILAGE fermentation, CORN as food
Abstract

BACKGROUND Fumonisin B1 ( FB1) is a mycotoxin produced by several Fusarium species and is a very common contaminant of maize-based food and feed throughout the world. The selection and use of FB1-degrading microorganisms appears as a promising alternative to cope with the problem of toxicity towards humans and livestock. High moisture maize grain silage, which is based on natural maize fermentation, could be an interesting reservoir of such microorganisms. RESULTS Using an in vitro simulated silage model with FB1 naturally contaminated grains, we demonstrated a significant raw decrease in FB1 during ensiling process ascribed to biodegradation mechanisms. A panel of 98 bacteria and yeasts were isolated from this matrix and selected for their ability to use FB1 as the sole source of C and N. For nine of them, the ability to degrade FB1 in vitro was evidenced. Notably, two bacteria identified as Lactobacillus sp. were highlighted for their efficient FB1-degrading capacity and production of hydrolysed FB1 as intermediate degradation metabolite. CONCLUSION Fermentation of high moisture maize grain contaminated with FB1 leads to a significant reduction of the toxin and allows the isolation of FB1-degrading microorganisms that could further be used as FB1 decontaminating agents. © 2016 Society of Chemical Industry [ABSTRACT FROM AUTHOR]

Published
2017
Full Text
View/download PDF

37. Yeast and bacteria from ensiled high moisture maize grains as potential mitigation agents of fumonisin B1.

Author

Martinez Tuppia, Ccori, Atanasova‐Penichon, Vessela, Chéreau, Sylvain, Ferrer, Nathalie, Marchegay, Gisèle, Savoie, Jean‐Michel, and Richard‐Forget, Florence

Subjects
*BIODEGRADATION, *MYCOTOXINS, *FUMONISINS, *SILAGE fermentation, *CORN as food
Abstract

BACKGROUND Fumonisin B1 ( FB1) is a mycotoxin produced by several Fusarium species and is a very common contaminant of maize-based food and feed throughout the world. The selection and use of FB1-degrading microorganisms appears as a promising alternative to cope with the problem of toxicity towards humans and livestock. High moisture maize grain silage, which is based on natural maize fermentation, could be an interesting reservoir of such microorganisms. RESULTS Using an in vitro simulated silage model with FB1 naturally contaminated grains, we demonstrated a significant raw decrease in FB1 during ensiling process ascribed to biodegradation mechanisms. A panel of 98 bacteria and yeasts were isolated from this matrix and selected for their ability to use FB1 as the sole source of C and N. For nine of them, the ability to degrade FB1 in vitro was evidenced. Notably, two bacteria identified as Lactobacillus sp. were highlighted for their efficient FB1-degrading capacity and production of hydrolysed FB1 as intermediate degradation metabolite. CONCLUSION Fermentation of high moisture maize grain contaminated with FB1 leads to a significant reduction of the toxin and allows the isolation of FB1-degrading microorganisms that could further be used as FB1 decontaminating agents. © 2016 Society of Chemical Industry [ABSTRACT FROM AUTHOR]

Published
2017
Full Text
View/download PDF

38. Yeast and bacteria from ensiled high moisture maize grains as potential mitigation agents of fumonisin B 1 .

Author

Martinez Tuppia C, Atanasova-Penichon V, Chéreau S, Ferrer N, Marchegay G, Savoie JM, and Richard-Forget F

Subjects
Bacteria classification, Bacteria genetics, Bacteria isolation & purification, Fermentation, Food Contamination analysis, Fumonisins analysis, Seeds chemistry, Silage analysis, Silage microbiology, Water analysis, Yeasts classification, Yeasts genetics, Yeasts isolation & purification, Zea mays chemistry, Bacteria metabolism, Fumonisins metabolism, Seeds microbiology, Yeasts metabolism, Zea mays microbiology
Abstract

Background: Fumonisin B 1 (FB 1 ) is a mycotoxin produced by several Fusarium species and is a very common contaminant of maize-based food and feed throughout the world. The selection and use of FB 1 -degrading microorganisms appears as a promising alternative to cope with the problem of toxicity towards humans and livestock. High moisture maize grain silage, which is based on natural maize fermentation, could be an interesting reservoir of such microorganisms., Results: Using an in vitro simulated silage model with FB 1 naturally contaminated grains, we demonstrated a significant raw decrease in FB 1 during ensiling process ascribed to biodegradation mechanisms. A panel of 98 bacteria and yeasts were isolated from this matrix and selected for their ability to use FB 1 as the sole source of C and N. For nine of them, the ability to degrade FB 1 in vitro was evidenced. Notably, two bacteria identified as Lactobacillus sp. were highlighted for their efficient FB 1 -degrading capacity and production of hydrolysed FB 1 as intermediate degradation metabolite., Conclusion: Fermentation of high moisture maize grain contaminated with FB 1 leads to a significant reduction of the toxin and allows the isolation of FB 1 -degrading microorganisms that could further be used as FB 1 decontaminating agents. © 2016 Society of Chemical Industry., (© 2016 Society of Chemical Industry.)

Published
2017
Full Text
View/download PDF

39. Metabolomics in food analysis: application to the control of forbidden substances.

Author

Dervilly-Pinel G, Courant F, Chéreau S, Royer AL, Boyard-Kieken F, Antignac JP, Monteau F, and Le Bizec B

Subjects
Anabolic Agents blood, Anabolic Agents urine, Animals, Growth Hormone analysis, Growth Hormone blood, Growth Hormone metabolism, Growth Hormone urine, Steroids blood, Steroids urine, Substance Abuse Detection methods, Anabolic Agents analysis, Anabolic Agents metabolism, Food Analysis methods, Metabolomics methods, Steroids analysis, Steroids metabolism, Substance Abuse Detection veterinary
Abstract

Metabolomics is a science of interest in food analysis to describe and predict properties of food products and processes. It includes the development of analytical methods with the ultimate goal being the identification of so-called 'quality markers', (i.e. sets of metabolites that correlate with, for example, quality, safety, taste, or fragrance of foodstuffs). In turn, these metabolites are influenced by factors as genetic differences of the raw food ingredients (such as animal breed or crop species differences), growth conditions (such as climate, irrigation strategy, or feeding) or production conditions (such as temperature, acidity, or pressure). In cases where the routine-based measurement of a food property faces some limitations such as the lack of knowledge regarding the target compounds to monitor, monitoring based on a limited set of crucial biomarkers is a good alternative, which is of great interest for food safety purposes regarding growth promoting practices. Such an approach may be more efficient than using a classic approach based on a limited set of known metabolites of anabolic compounds. In this context, screening strategies allowing detection of the physiological response resulting from anabolic compound administration are promising approaches to detect their misuse. The global metabolomics workflow implemented for such studies is presented and illustrated through various examples of biological matrices profiling (tissue, blood, urine) and for different classes of anabolic compounds (steroids, β-agonists and somatotropin)., (© 2012 John Wiley & Sons, Ltd.)

Published
2012
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results