Search

Your search keyword '"Cha, Se-Yeoun"' showing total 172 results
Start Over Author "Cha, Se-Yeoun"
172 results on '"Cha, Se-Yeoun"'

2. Establishment and Application of Mismatch Amplification Mutation Assay-PCR for Rapid Detection and Differentiation of Duck Hepatitis A Virus-1 Attenuated Vaccine and Wild Strains.

Author

Yu, Cheng-Dong, Choi, Yu-Ri, Park, Jong-Yeol, Kim, Sang-Won, Cha, Se-Yeoun, Jang, Hyung-Kwan, Kang, Min, and Wei, Bai

Subjects
SINGLE nucleotide polymorphisms, HEPATITIS viruses, HEPATITIS A virus, VACCINE safety, DUCKLINGS, VIRAL hepatitis
Abstract

Simple Summary: Duck hepatitis A virus (DHAV) is an infectious pathogen that causes fatal viral hepatitis in ducklings. Duck hepatitis A virus-1 (DHAV-1) is the most virulent and widely distributed serotype, inflicting severe damage on the global duck farming industry. The main approach to preventing DHAV-1 involves using attenuated live vaccines. However, these vaccine strains may persist in ducks and pose a risk of reverting to virulence. Therefore, distinguishing between infected and vaccinated animals is vital for clinical diagnosis and vaccine safety monitoring. Currently, only time-consuming and costly sequencing methods serve this purpose. In this study, a Mismatch Amplification Mutation Assay (MAMA)-PCR method was developed based on single nucleotide polymorphism (SNPs) in the VP1 gene. The MAMA-PCR method offers a highly specific and sensitive clinical tool for rapidly distinguishing DHAV-1 wild-type from vaccine strains, significantly contributing to accurate clinical diagnosis and epidemiological understanding. Duck hepatitis A virus type 1 (DHAV-1) is the main pathogen causing viral hepatitis in ducks, marked by high contagion and acute mortality. Live attenuated DHAV-1 vaccines are widely used to control the disease. This study aims to develop a mismatch amplification mutation assay (MAMA)-PCR for the rapid detection and differentiation of Korean DHAV-1 wild-type strains from vaccine strains. A MAMA primer was designed to target a single nucleotide polymorphism (SNPs) at position 2276 within the VP1 gene, allowing differentiation in a single PCR reaction. The MAMA-PCR accurately identified both strains, with detection limits of 100.5 ELD50/mL and 102.3 ELD50/mL, respectively. The MAMA-PCR demonstrated specificity, showing no cross-reactivity with 12 other viral and bacterial pathogens. The MAMA-PCR was applied to 89 farms, yielding results consistent with nested-PCR and sequence determination, identifying four positive farms for DHAV-1 vaccine strains. In conclusion, this study is the first to employ the MAMA-PCR method to distinguish between DHAV-1 wild-type and vaccine strains. The developed method is rapid, simple, specific, and sensitive, thereby serving as an effective tool for clinical diagnostics in identifying and differentiating between Korean DHAV-1 wild-type and vaccine strains. [ABSTRACT FROM AUTHOR]

Published
2024
Full Text
View/download PDF

7. Genetic Characterization of Antigenic Variant Infectious Bursal Disease Virus (IBDV) in Chickens in Korea

Author

Park, Jong-Yeol, primary, Kim, Ki-Woong, additional, Shang, Ke, additional, Kim, Sang-Won, additional, Choi, Yu-Ri, additional, Yu, Cheng-Dong, additional, Son, Ji-Eun, additional, Kim, Gyeong-Jun, additional, Jeon, Won-Bin, additional, Kim, In-Hwan, additional, Wei, Bai, additional, Kang, Min, additional, Jang, Hyung-Kwan, additional, and Cha, Se-Yeoun, additional

Published
2023
Full Text
View/download PDF

9. Comparative Studies of Antimicrobial Resistance in Escherichia coli , Salmonella , and Campylobacter Isolates from Broiler Chickens with and without Use of Enrofloxacin.

Author

Shang, Ke, Kim, Ji-Hyuk, Park, Jong-Yeol, Choi, Yu-Ri, Kim, Sang-Won, Cha, Se-Yeoun, Jang, Hyung-Kwan, Wei, Bai, and Kang, Min

Subjects
BROILER chickens, DRUG resistance in microorganisms, CAMPYLOBACTER, ESCHERICHIA coli, CAMPYLOBACTER jejuni, ENTEROBACTERIACEAE, SALMONELLA, FLUOROQUINOLONES
Abstract

This study investigated the effect of enrofloxacin (ENR) administration on the prevalence and antimicrobial resistance of E. coli, Salmonella, and Campylobacter isolated from broiler chickens under field conditions. The isolation rate of Salmonella was significantly lower (p < 0.05) on farms that administered ENR (6.4%) than on farms that did not (11.6%). The Campylobacter isolation rate was significantly higher (p < 0.05) in farms that administered ENR (6.7%) than in farms that did not (3.3%). The ratio of resistance to ENR was significantly higher (p < 0.05) in E. coli isolates from farms that used ENR (88.1%) than farms that did not (78.0%). The respective ratio of resistance to ampicillin (40.5% vs. 17.9%), chloramphenicol (38.0% vs. 12.5%), tetracycline (63.3% vs. 23.2%), and trimethoprim/sulfamethoxazole (48.1% vs. 28.6%) and the ratio of intermediate resistance to ENR (67.1% vs. 48.2%) were significantly higher (p < 0.05) in Salmonella isolates from the farms that used ENR than farms that did not. In conclusion, the use of ENR at broiler farms was an important factor in decreasing the prevalence of Salmonella but not Campylobacter and caused ENR resistance among E. coli and Salmonella but not Campylobacter. Exposure to ENR could have a co-selective effect on antimicrobial resistance in enteric bacteria in the field. [ABSTRACT FROM AUTHOR]

Published
2023
Full Text
View/download PDF

31. The novel cathelicidin of naked mole rats, Hg-CATH, showed potent antimicrobial activity and low cytotoxicity

Author

Cho, Hye-sun, primary, Soundrarajan, Nagasundarapandian, additional, Le Van Chanh, Quy, additional, Jeon, Hyoim, additional, Cha, Se-Yeoun, additional, Kang, Mingue, additional, Ahn, ByeongYong, additional, Hong, Kwonho, additional, Song, Hyuk, additional, Kim, Jin-Hoi, additional, Oh, Kyung-Soo, additional, and Park, Chankyu, additional

Published
2018
Full Text
View/download PDF

33. Genomewide Analysis of the Antimicrobial Peptides in Python bivittatus and Characterization of Cathelicidins with Potent Antimicrobial Activity and Low Cytotoxicity

Author

Kim, Dayeong, primary, Soundrarajan, Nagasundarapandian, additional, Lee, Juyeon, additional, Cho, Hye-sun, additional, Choi, Minkyeung, additional, Cha, Se-Yeoun, additional, Ahn, Byeongyong, additional, Jeon, Hyoim, additional, Le, Minh Thong, additional, Song, Hyuk, additional, Kim, Jin-Hoi, additional, and Park, Chankyu, additional

Published
2017
Full Text
View/download PDF

46. Molecular Characterization of Fluoroquinolone Resistance Mechanisms of CampylobacterIsolates from Duck Meats

Author

Kang, Min, Wei, Bai, Choi, Sung-Woon, Cha, Se-Yeoun, and Jang, Hyung-Kwan

Abstract

The purpose of this study was to identify the molecular basis of quinolone resistance of Campylobacterisolates recovered from duck meats. Sixty-one isolates from duck meat samples were studied using sequence analysis of the gyrAgene, and PCR assays were used to identify the presence of the CmeABC efflux pump and its restored sensitivity in the presence of efflux-pump inhibitors. High-level resistance to nalidixic acid and ciprofloxacin was attributed to amino acid substitutions Thr-86-Ile in some isolates. The PCR assay confirmed the presence of the cmeBgene in 29 (47.5%) of the 61 Campylobacterisolates. Phenylalanine arginine β-naphthylamide reduced the MICs of ciprofloxacin and nalidixic acid in 16 (55.2%) and 26 (89.7%) isolates, respectively. The Thr-86-Ile substitution in the gyrAwas the primary contributor to the high-level quinolone resistance in Campylobacterisolates from duck meats.

Published
2017
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results