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Comparative dietary response of different isolated strains (Direct fed microbial- DFM) of lactobacilli, streptococci and yeast isolated from leopard feces (Panthera leo) was studied as probiotic in poultry broiler birds reared as per varied mineral densities viz. calcium and phosphorus. Various treatments consisted of T0 (Control, culture medium), T1 (Lactobacillus casei + Streptococcus fecalis + Saccharomyces cerevisiae) offered standard formulated diet D1, T2 - Control (Culture medium) offered ten percent lower calcium and phosphorus diet D2, T3 (Lactobacillus casei + Streptococcus fecalis + Saccharomyces cerevisiae) offered ten percent lower calcium and phosphorus diet D2. Growth results obtained during the starter phase (1st to 3rd week) exhibited higher gain in live body weight and lower feed conversion ratio, both by DFM supplemented treatment T1 offered standard formulated diet D1 and treatment T3 offered experimentally formulated mineral (Ca and P) deficient (10%) diet D2 compared to respective control T0 and T2. The obtained results revealed a significantly higher growth performance in treatment T1 compared to its control T0 where a higher growth in treatment T3 offered experimentally formulated mineral (Ca and P) deficient (10%) diet D2 supplemented with isolated DFM compared to its control T2 was exhibited. Treatment groups T1 and T3 supplemented with isolated DFM exhibited a better intestinal micro flora balance, effective colonization and higher count in the intestinal tract with higher calcium and phosphorus retention in the digestibility studies. Thus, it was found that supplementation of isolated DFM has the potential to improve biological growth performance of poultry broiler birds offered both standard formulated diet D1, as well as mineral deficient diet D2. [Projekat Ministarstva nauke Republike Srbije, br. III 46002]

Genetically diverse bell pepper cultivars and their 30 F1 hybrids were developed by means of diallel mating system including reciprocals. Variance analysis illustrated considerable disparity among newly developed bacterial wilt resistant hybrid lines for all the studied traits. General Combining ability (gca) effects were more pronounced than specific combining ability effects (sca). The gca effects were high with parents DPBWRC-6-1, EC-464107, EC-464115 and DPBWRC-39 for fruit yield and yield-enhancing traits. Further, reciprocal effects influenced all parameters except fruit width, TSS and lobes per fruit. Among the additive and non-additive genetic variance, the latter had a more significant influence on the inheritance of all studied characters except for fruit width and pericarp thickness. Based on sca analysis, three cross-combinations viz., DPBWRC-29×EC-464107, DPBWRC-39× DPBWRC-1 and EC-464115 × DPBWRC-29 were found to be good specific combiners for the majority of traits and can further be utilized to isolate superior segregates or released as hybrids. [ABSTRACT FROM AUTHOR]

Summary: Chlamydia suis has been detected in numerous disease conditions of pigs, particularly in eye infections. This study examined recurring conjunctivitis cases in five commercial pig farms in Japan. 40.5% of the cases were identified as Chlamydia positive using impression cytology of ocular smears and a genus‐specific direct fluorescent antibody. C. suis was detected in 59.5% of the samples using PCR tests targeting 16S‐23S rRNA intergenic spacer region (ISR) and ompA gene. Genetic analysis of PCR amplicons revealed nine sequence variants of 16S‐23S rRNA ISR and 20 sequence variants within ompA gene. Among C. suis‐positive conjunctivitis cases, 36.4% showed concurrent infection with 2–4 varied ompA sequence types and 9.1% showed multiple 16S‐23S rRNA ISR sequence types of C. suis. Multiple genotypes were found circulating in four of five farms. All 20 detected strains and 25 previously reported C. suis strains were grouped into four clusters. Japanese C. suis strains were closely related to American and European strains indicating wide distribution of these genetically variant strains. This study is the first to show multiple and genetically diverse C. suis strain associations in pig conjunctivitis. [ABSTRACT FROM AUTHOR]

Genetic improvement mainly depends on the extent of genetic variability present in the population. The molecular marker is a useful tool for assessing genetic variations and resolving cultivar identities. The objective of this study was to evaluate the genetic divergence of 40 germplasm lines of ricebean. Forty-four SSR markers were used in the present study, out of which only seven were found to be polymorphic. The dendrogram based on SSR marker analysis grouped the 40 ricebean genotypes into 2 clusters, where cluster I was the largest with 37 genotypes. STRUCTURE analysis for the estimation of population structure divided 40 genotypes into 4 groups in which P1 group consisted of 5 genotypes, P2 with 23 genotypes, P3 with maximum number of 7 genotypes, whereas, P4 consisted of 5 genotypes. The genetic variation revealed by SSR analysis will be used in the future breeding programme of ricebean for the selection of diverse parents for hybridization. [ABSTRACT FROM AUTHOR]

Pasteurella multocida (P. multocida) is an important pathogen of various domestic animals. The outer membrane proteins (OMPs) play a major role in pathogenesis and immunogenicity of P. multocida. The aim of the study was to develop indirect enzyme linked immuno sorbant assay (ELISA) based on OMPs to ascertain the antibody titers in animals post-infection or to gauge the potency of vaccine. The OMPs were extracted and purified from P. multocida P:52 (vaccine strain) and P. multocida B:2 isolated from natural outbreak of Haemorrhagic septicaemia (HS) and analyzed on SDS PAGE and through western blot. The OMPs profile of the vaccine strain and the isolate from the natural outbreak of HS were found to be similar. Optimization of various components viz. coating antigens, anti-species conjugate, etc. were carried out against both anti-P. multocida hyper immune and pre immune serum. Validation of OMP based indirect ELISA assay to measure immune response against P. multocida in bovine revealed 91% diagnostic sensitivity (DSN) and about 100% diagnostic specificity (DSP) at 25% cut off. OMP based indirect ELISA was found to be more specific, but less sensitive as compared to WCL based assay. [ABSTRACT FROM AUTHOR]

The study was undertaken to develop and identify high yielding and better quality cultivars through line x tester analysis. Seventeen genotypes and 30 F1 hybrids obtained by crossing 15 lines and 2 testers in line x tester mating system were sown in randomized complete block design. Significant differences were observed among parents for all the traits except seeds per pod. Lines L-658, L-666, L-407, L-354 and PL-406 were good general combiners for seed yield and most of its components, whereas L-617, L-737, L-635, L-412 were good general combiners for yield and quality components and L-649 was good general combiner for earliness. The specific cross combinations Vipasa x L-412, PL-406 x L-617, Vipasa x L-620, PL-406 x L-651, PL-406 x L-630, Vipasa x L-649 and PL-406 x L-635 were outstanding for seed yield and most of its components. Based on per se performance, sca effects and standard heterosis, cross combination PL-406 x L-649 was best for days to 50% flowering; PL-406 x L-651 best for pods per plant; Vipasa x L-652 and PL-406 x L-354 were observed to be best for protein content. The early flowering genotypes (PL-406 x L-649) identified in the present study may be exploited for the development of drought tolerant lentil genotypes. [ABSTRACT FROM AUTHOR]

Eleven agro-morphological characters were studied for variability and their inter-relationships using 95 advanced (F6) elite derivatives of microsperma x macrosperma crosses along with 5 checks namely Vipasa, EC-1(Markandya), PL-639, L-259, and L-4145 at Palampur. The present investigation revealed sufficient variability for all the characters except seeds per pod. For 100- seed weight, pods per plant and biological yield per plant, high heritability coupled with high genetic advance indicated the predominant role of additive gene action in the inheritance of these traits. On the basis of mean performance of different genotypes, it was observed that lines HPCL-2290, HPCL-2301 and HPCL-2208 were superior to best check Vipasa for seed yield per plant, pods per plant and number of fertile nodes per plant. Seed yield per plant showed positive association with biological yield per plant, seeds per pod, pods per plant, number of fertile nodes per plant, primary branches per plant and plant height. Path analysis revealed that biological yield had highest positive direct effect followed by harvest index on the seed yield. Biological yield not only had highest direct effect but several other traits, like pods per plant, number of fertile nodes per plant, primary branches per plant and plant height had also contributed significantly indirectly through it. Based on the present study a plant ideotype with higher biomass, harvest index, pods per plant, number of fertile nodes per plant, primary branches per plant and plant height would enhance the seed yield of lentil. [ABSTRACT FROM AUTHOR]

Comparative dietary response of different isolated strains (Direct fed microbial-DFM) of lactobacilli, streptococci and yeast isolated from leopard feces (Panthera leo) was studied as probiotic in poultry broiler birds reared as per varied mineral densities viz. calcium and phosphorus. Various treatments consisted of T0 (Control, culture medium), T1 (Lactobacillus casei + Streptococcus fecalis + Saccharomyces cerevisiae) offered standard formulated diet D1, T2 - Control (Culture medium) offered ten percent lower calcium and phosphorus diet D2, T3 (Lactobacillus casei + Streptococcus fecalis + Saccharomyces cerevisiae) offered ten percent lower calcium and phosphorus diet D2. Growth results obtained during the starter phase (1st to 3rd week) exhibited higher gain in live body weight and lower feed conversion ratio, both by DFM supplemented treatment T1 offered standard formulated diet D1 and treatment T3 offered experimentally formulated mineral (Ca and P) deficient (10%) diet D2 compared to respective control T0 and T2. The obtained results revealed a significantly higher growth performance in treatment T1 compared to its control T0 where a higher growth in treatment T3 offered experimentally formulated mineral (Ca and P) deficient (10%) diet D2 supplemented with isolated DFM compared to its control T2 was exhibited. Treatment groups T1 and T3 supplemented with isolated DFM exhibited a better intestinal micro flora balance, effective colonization and higher count in the intestinal tract with higher calcium and phosphorus retention in the digestibility studies. Thus, it was found that supplementation of isolated DFM has the potential to improve biological growth performance of poultry broiler birds offered both standard formulated diet D1, as well as mineral deficient diet D2. [ABSTRACT FROM AUTHOR]

Ankyrin repeat is a 33-amino acid motif commonly observed in eukaryotes and, to a lesser extent, in prokaryotes and archaea and rarely in viruses. This motif plays a crucial role in regulating various cellular processes like the cell cycle, transcription, cell signaling, and inflammatory responses through interactions between proteins. Poxviruses exhibit a distinctive feature of containing multiple ankyrin repeat proteins within their genomes. All the genera of poxviruses possess these proteins except molluscipox virus, crocodylidpox virus, and red squirrel poxvirus. An intriguing characteristic has generated notable interest in studying the functions of these proteins within poxvirus biology. Within poxviruses, ankyrin repeat proteins exhibit a distinct configuration, featuring ankyrin repeats in the N-terminal region and a cellular F-box homolog in the C-terminal region, which enables interactions with the cellular Skp, Cullin, F-box containing ubiquitin ligase complex. Through the examination of experimental evidences and discussions from current literature, this review elucidates the organization and role of ankyrin repeat proteins in poxviruses. Various research studies have highlighted the significant importance of these proteins in poxviral pathogenesis and, acting as factors that enhance virulence. Consequently, they represent viable targets for developing genetically altered viruses with decreased virulence, thus displaying potential as candidates for vaccines and antiviral therapeutic development contributing to safer and more effective strategies against poxviral infections., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024. Published by Elsevier B.V.)

Aeromonas spp. are normal inhabitants of aquatic environments and are emerging foodborne bacterial pathogens. Aeromonas spp. contamination is frequent in ready-to-eat (RTE) seafood and can also occur in products prepared from milk or meat. The study determined the enterotoxin and antimicrobial susceptibility profiles of Aeromonas spp. isolates recovered from RTE milk products (n = 105), RTE meat/fish products (n = 40) and drinking water (n = 60) samples collected from tourist places in Himachal Pradesh, India, in northwestern Himalayas. 7.3 % (16/220) samples were found contaminated with Aeromonas spp. These isolates were identified as A. hydrophila (31.3 %), A. schubertii (25.0 %), A. sobria (25.0 %) and A. veronii (18.8 %). Aeromonas spp. contamination was significantly higher (14.3 %, 15/105, p = 0.0001) in RTE milk products. The contamination levels for water samples were 1.7 % whereas none of the tested RTE meat or fish products yielded Aeromonas spp. Among RTE milk products, contamination was significantly higher in paneer (South Asian soft cheese) (26.1 %, p = 0.0027) and cream (25.0 %, p = 0.046) based RTE foods. All isolates carried alt (361 bp), encoding a cytotonic heat-labile enterotoxin. Ampicillin resistance was 100 % and high levels (>30 %) of resistance were recorded for amoxicillin/clavulanic acid, amikacin, cefotaxime and ceftazidime. Six (37.5 %) isolates were multi drug resistant (MDR), showing resistance to aminoglycosides, cephams and penicillins. Isolation of alt carrying MDR isolates from RTE foods indicates that Aeromonas spp. can be potential foodborne public health threat in northwestern Himalayas., Competing Interests: Declaration of competing interest The authors declare that they have no conflict of interest., (Copyright © 2024. Published by Elsevier B.V.)

The members of family Chlamydiaceae have a broad host range and cause many kinds of diseases in humans and animals. Several cases of Chlamydiaceae being detected in atypical hosts have been reported recently. Consequently, cross-species monitoring of Chlamydia in wildlife and livestock is pertinent for public health, animal hygiene and wildlife conservation. In this study, we conducted molecular surveillance of Chlamydia in wild birds and livestock around a small village in the foothills of Mt. Afadjato, Ghana where direct contact between wildlife and livestock occurs. Among 29 captured wild birds and 63 livestock, 5 sheep, 30 goats and 28 chickens, the positive ratios of Chlamydia were 24.1%, 40.0%, 43.3% and 26.9%, respectively. Chlamydia pecorum was detected in wild birds, goats, sheep and chickens. On the basis of the variable domain 2 region of ompA, several samples from different hosts showed identical sequences and were phylogenetically located to the same clusters. In addition, using ompA, C. psittaci, C. abortus and C. gallinacea were also detected in this small habitat. Further genetic and pathogenic analyses of the chlamydial distribution in this area, which represents the interface of wild and domestic animal interactions, may improve our knowledge of their transmission among different hosts.

An outbreak of sheeppox was investigated in a cluster of villages situated in Western Himalayan ranges of a Northern Indian state. Non-migratory sheep (n = 80) of native breeds namely Gaddi and Rampur Bushair were infected and 15 have died. The outbreak started after a few animals contracted the disease during the summer grazing period at the highland pastures from migrating flocks of sheep. This initial outbreak resulted in a further spreading of the disease into the valley. Clinical examination revealed varying degree of cutaneous papular lesions and respiratory distress. Upon necropsy, visceral lesions in the lungs, trachea and kidneys were also found. Clinical and morbid samples were found positive for sheeppox virus using group specific P32 gene and I3L gene based multiplex PCR differentiating sheeppox and goatpox viruses. Histopathological, hematological and blood biochemical analysis also supported the pathology of an acute viral infection. The causative sheeppox virus strain was isolated using lamb testicular cell culture and phylogenetic analysis, based upon P32 and RPO30 genes, showed its clustering with other Indian strains reported from neighboring states. This study demonstrated the spread of sheeppox virus to new niches by migratory sheep flocks leading to establishment of endemic infections in many new pockets of higher Western Himalayas., (© 2021. The Author(s), under exclusive licence to Springer Nature B.V.)