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The poor prognosis of immunotherapy in patients with colorectal cancer (CRC) necessitates a comprehensive understanding of the immunosuppressive mechanisms within tumor microenvironment (TME). Undoubtedly, the anti-tumor immune cells play an indispensable role in immune tolerance. Therefore, it is imperative to investigate novel immune-related factors that have the capacity to enhance anti-tumor immunity. Here, we employed bioinformatic analysis using R and Cytoscape to identify the hub gene chemokine (C-X-C motif) ligand 8 (CXCL8), which is overexpressed in CRC, in the malignant progression of CRC. However, its specific role of CXCL8 in CRC immunity remains to be elucidated. For this purpose, we evaluated how tumor-derived CXCL8 promotes M2 macrophage infiltration by in vivo and in vitro, which can be triggered by IL-1β within TME. Mechanistically, CXCL8-induced polarization of M2 macrophages depends on the activation of the STAT3 signaling. Finally, immunohistochemistry and multiplexed immunohistochemistry analysis identified that CXCL8 not only enhances PD-L1 + M2 macrophage infiltration but also attenuates the recruitment of PD-1 + CD8 + T cells in murine CRC models. Together, these findings emphasize the critical role for CXCL8 in promoting M2 macrophage polarization and inhibiting CD8 + T cell infiltration, thereby links CXCL8 to the emergency of immunosuppressive microenvironment facilitating tumor evasion. Overall, these findings may provide novel strategy for CRC immunotherapy., (© 2023 The Authors. The FASEB Journal published by Wiley Periodicals LLC on behalf of Federation of American Societies for Experimental Biology.)

In this paper, various hollow structured optical fields are generated by skillfully adjusting the number and positions of multiple off-axis vortices loaded in a Gaussian beam. The focal-field characteristics of the generated hollow structured optical fields after passing through an ordinary lens are studied based on the scalar diffraction theory. Firstly, a variety of hollow structured optical fields are theoretically simulated by adjusting the number and positions of multiple off-axis vortices loaded in the Gaussian beam. The focal-field characteristics of the hollow structured optical fields after passing through a lens are theoretically analyzed. On this basis, the experiments are implemented in the built optical system for multi-off-axis vortex beam focusing through an ordinary lens. In the experiments, various hollow structured optical fields are detected in CCD which are consistent with the theoretical results. The manipulations of size and rotation direction of the hollow structured optical fields are realized. We believe that this study will contribute to extending the potential applications of off-axis vortex beams in fields such as optical field shaping, optical manipulation and laser processing.