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1. A fully convolutional neural network for comprehensive compartmentalization of abdominal adipose tissue compartments in MRI

Author

Kway, Yeshe M., Thirumurugan, Kashthuri, Michael, Navin, Tan, Kok Hian, Godfrey, Keith M., Gluckman, Peter, Chong, Yap Seng, Venkataraman, Kavita, Khoo, Eric Yin Hao, Khoo, Chin Meng, Leow, Melvin Khee-Shing, Tai, E Shyong, Chan, Jerry KY., Chan, Shiao-Yng, Eriksson, Johan G., Fortier, Marielle V., Lee, Yung Seng, Velan, S. Sendhil, Feng, Mengling, and Sadananthan, Suresh Anand

Published
2023
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2. Time-to-conception and clinical pregnancy rate with a myo-inositol, probiotics, and micronutrient supplement: secondary outcomes of the NiPPeR randomized trial

Author

Albert, Ben, Cai, Shirong, Calder, Philip C., Carvalho, Ryan, Guiao Castro, Julie Ann, Cavanagh, Mary, Chan, Jerry KY., Chang, Mei Ling, Chi, Claudia, Childs, Caroline E., Choh, Mei Kit, Chong, Mary FF., Chu, Anne HY., Conlon, Cathryn, Paula Costello, Cyrus Cooper, Cox, Vanessa, Galani, Sevasti, Hammond, Judith, Harvey, Nicholas C., Holt, Richard, Inskip, Hazel M., Jagtap, Mrunalini, Jeon, Gene, Karnani, Neerja, Nembrini, Chiara, Lillycrop, Karen A., Müller-Riemenschneider, Falk, Natarajan, Padmapriya, Ng, Sharon, Ramasamy, Adaikalavan, Tham, Elizabeth, Tint, Mya Thway, O’Sullivan, Justin M., Satianegara, Gernalia, Shek, Lynette PC., Silva-Zolezzi, Irma, Sim, Wendy, Soh, Shu E., Tay, Vicky, Taylor, Rachel, Theodosia, Salika, Wall, Clare, Woon, Gladys, Vickers, Mark, Ying, Wei, Chan, Shiao-Yng, Barton, Sheila J., Loy, See Ling, Chang, Hsin Fang, Titcombe, Philip, Wong, Jui-Tsung, Ebreo, Marilou, Ong, Judith, Tan, Karen ML., Nield, Heidi, El-Heis, Sarah, Kenealy, Timothy, Chong, Yap-Seng, Baker, Philip N., Cutfield, Wayne S., and Godfrey, Keith M.

Published
2023
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3. CXCR4 identifies transitional bone marrow premonocytes that replenish the mature monocyte pool for peripheral responses

Author

Chong, Shu Zhen, Evrard, Maximilien, Devi, Sapna, Chen, Jinmiao, Lim, Jyue Yuan, See, Peter, Zhang, Yiru, Adrover, José M, Lee, Bernett, Tan, Leonard, Li, Jackson LY, Liong, Ka Hang, Phua, Cindy, Balachander, Akhila, Boey, Adrian, Liebl, David, Tan, Suet Mien, Chan, Jerry KY, Balabanian, Karl, Harris, John E, Bianchini, Mariaelvy, Weber, Christian, Duchene, Johan, Lum, Josephine, Poidinger, Michael, Chen, Qingfeng, Rénia, Laurent, Wang, Cheng-I, Larbi, Anis, Randolph, Gwendalyn J, Weninger, Wolfgang, Looney, Mark R, Krummel, Matthew F, Biswas, Subhra K, Ginhoux, Florent, Hidalgo, Andrés, Bachelerie, Françoise, and Ng, Lai Guan

Subjects
Biomedical and Clinical Sciences, Clinical Sciences, Rare Diseases, Good Health and Well Being, Animals, Antigens, Ly, Bone Marrow Cells, Cell Differentiation, Circadian Rhythm, Endotoxins, Female, Gene Expression Profiling, Lung, Mice, Inbred C57BL, Monocytes, Receptors, CXCR4, Medical and Health Sciences, Immunology, Biomedical and clinical sciences, Health sciences
Abstract

It is well established that Ly6Chi monocytes develop from common monocyte progenitors (cMoPs) and reside in the bone marrow (BM) until they are mobilized into the circulation. In our study, we found that BM Ly6Chi monocytes are not a homogenous population, as current data would suggest. Using computational analysis approaches to interpret multidimensional datasets, we demonstrate that BM Ly6Chi monocytes consist of two distinct subpopulations (CXCR4hi and CXCR4lo subpopulations) in both mice and humans. Transcriptome studies and in vivo assays revealed functional differences between the two subpopulations. Notably, the CXCR4hi subset proliferates and is immobilized in the BM for the replenishment of functionally mature CXCR4lo monocytes. We propose that the CXCR4hi subset represents a transitional premonocyte population, and that this sequential step of maturation from cMoPs serves to maintain a stable pool of BM monocytes. Additionally, reduced CXCR4 expression on monocytes, upon their exit into the circulation, does not reflect its diminished role in monocyte biology. Specifically, CXCR4 regulates monocyte peripheral cellular activities by governing their circadian oscillations and pulmonary margination, which contributes toward lung injury and sepsis mortality. Together, our study demonstrates the multifaceted role of CXCR4 in defining BM monocyte heterogeneity and in regulating their function in peripheral tissues.

Published
2016

4. Time-to-conception and clinical pregnancy rate with a myo-inositol, probiotics, and micronutrient supplement: secondary outcomes of the NiPPeR randomized trial

Author

Chan, Shiao-Yng, primary, Barton, Sheila J., additional, Loy, See Ling, additional, Chang, Hsin Fang, additional, Titcombe, Philip, additional, Wong, Jui-Tsung, additional, Ebreo, Marilou, additional, Ong, Judith, additional, Tan, Karen ML., additional, Nield, Heidi, additional, El-Heis, Sarah, additional, Kenealy, Timothy, additional, Chong, Yap-Seng, additional, Baker, Philip N., additional, Cutfield, Wayne S., additional, Godfrey, Keith M., additional, Albert, Ben, additional, Cai, Shirong, additional, Calder, Philip C., additional, Carvalho, Ryan, additional, Guiao Castro, Julie Ann, additional, Cavanagh, Mary, additional, Chan, Jerry KY., additional, Chang, Mei Ling, additional, Chi, Claudia, additional, Childs, Caroline E., additional, Choh, Mei Kit, additional, Chong, Mary FF., additional, Chu, Anne HY., additional, Conlon, Cathryn, additional, Paula Costello, Cyrus Cooper, additional, Cox, Vanessa, additional, Galani, Sevasti, additional, Hammond, Judith, additional, Harvey, Nicholas C., additional, Holt, Richard, additional, Inskip, Hazel M., additional, Jagtap, Mrunalini, additional, Jeon, Gene, additional, Karnani, Neerja, additional, Nembrini, Chiara, additional, Lillycrop, Karen A., additional, Müller-Riemenschneider, Falk, additional, Natarajan, Padmapriya, additional, Ng, Sharon, additional, Ramasamy, Adaikalavan, additional, Tham, Elizabeth, additional, Tint, Mya Thway, additional, O’Sullivan, Justin M., additional, Satianegara, Gernalia, additional, Shek, Lynette PC., additional, Silva-Zolezzi, Irma, additional, Sim, Wendy, additional, Soh, Shu E., additional, Tay, Vicky, additional, Taylor, Rachel, additional, Theodosia, Salika, additional, Wall, Clare, additional, Woon, Gladys, additional, Vickers, Mark, additional, and Ying, Wei, additional

Published
2023
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5. Longitudinal characterization of determinants associated with obesogenic growth patterns in early childhood

Author

Michael, Navin, Gupta, Varsha, Fogel, Anna, Huang, Jonathan, Chen, Li, Sadananthan, Suresh Anand, Ong, Yi Ying, Aris, Izzuddin M., Pang, Wei Wei, Yuan, Wen Lun, Loy, See Ling, Thway Tint, Mya, Tan, Kok Hian, Chan, Jerry Ky, Chan, Shiao-Yng, Shek, Lynette Pei-Chi, Yap, Fabian, Godfrey, Keith, Chong, Yap Seng, Gluckman, Peter, Velan, S.S., Forde, Ciarán G., Lee, Yung Seng, Eriksson, Johan G., Karnani, Neerja, Michael, Navin, Gupta, Varsha, Fogel, Anna, Huang, Jonathan, Chen, Li, Sadananthan, Suresh Anand, Ong, Yi Ying, Aris, Izzuddin M., Pang, Wei Wei, Yuan, Wen Lun, Loy, See Ling, Thway Tint, Mya, Tan, Kok Hian, Chan, Jerry Ky, Chan, Shiao-Yng, Shek, Lynette Pei-Chi, Yap, Fabian, Godfrey, Keith, Chong, Yap Seng, Gluckman, Peter, Velan, S.S., Forde, Ciarán G., Lee, Yung Seng, Eriksson, Johan G., and Karnani, Neerja

Abstract

Background: Longitudinal assessment of the determinants of obesogenic growth trajectories in childhood can suggest appropriate developmental windows for intervention.Methods: Latent class growth mixture modelling was used to identify body mass index (BMI) z-score trajectories from birth to age 6 years in 994 children from a prospective mother–offspring cohort (Chinese, Indian and Malay ethnicities) based in Singapore. We evaluated the early-life determinants of the trajectories as well as their associations with cardiometabolic risk markers at age 6 years.Results: Five BMI z-score trajectory patterns were identified, three within the healthy weight range, alongside early-acceleration and late-acceleration obesogenic trajectories. The early-acceleration pattern was characterized by elevated fetal abdominal circumference growth velocity, BMI acceleration immediately after birth and crossing of the obesity threshold by age 2 years. The late-acceleration pattern had normal fetal growth and BMI acceleration after infancy, and approached the obesity threshold by age 6 years. Abdominal fat, liver fat, insulin resistance and odds of pre-hypertension/hypertension were elevated in both groups. Indian ethnicity, high pre-pregnancy BMI, high polygenic risk scores for obesity and shorter breastfeeding duration were common risk factors for both groups. Malay ethnicity and low maternal educational attainment were uniquely associated with early BMI acceleration, whereas nulliparity and obesogenic eating behaviours in early childhood were uniquely associated with late BMI acceleration.Conclusion: BMI acceleration starting immediately after birth or after infancy were both linked to early cardiometabolic alterations. The determinants of these trajectories may be useful for developing early risk stratification and intervention approaches to counteract metabolic adversities linked to childhood obesity.

Published
2023

6. Longitudinal characterization of determinants associated with obesogenic growth patterns in early childhood

Author

Michael, Navin, primary, Gupta, Varsha, additional, Fogel, Anna, additional, Huang, Jonathan, additional, Chen, Li, additional, Sadananthan, Suresh Anand, additional, Ong, Yi Ying, additional, Aris, Izzuddin M, additional, Pang, Wei Wei, additional, Yuan, Wen Lun, additional, Loy, See Ling, additional, Thway Tint, Mya, additional, Tan, Kok Hian, additional, Chan, Jerry Ky, additional, Chan, Shiao-Yng, additional, Shek, Lynette Pei-Chi, additional, Yap, Fabian, additional, Godfrey, Keith, additional, Chong, Yap Seng, additional, Gluckman, Peter, additional, Velan, S Sendhil, additional, Forde, Ciarán G, additional, Lee, Yung Seng, additional, Eriksson, Johan G, additional, and Karnani, Neerja, additional

Published
2022
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8. Maternal Dendritic Cells Influence Fetal Allograft Response following Murine In-Utero Hematopoietic Stem Cell Transplantation

Author

Kandasamy, Karthikeyan, primary, Johana, Nuryanti Binti, additional, Tan, Lay Geok, additional, Tan, Yvonne, additional, Li, Julie Yeo Su, additional, Zhihui, Li, additional, Jiayu, Koh, additional, Ginhoux, Florent, additional, Chan, Jerry KY, additional, Choolani, Mahesh A, additional, and Mattar, Citra NZ, additional

Published
2022
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9. BNT162B2 COVID-19 mRNA vaccination did not promote substantial anti-syncytin-1 antibody production nor mRNA transfer to breast milk in an exploratory pilot study

Author

Mattar, Citra NZ, primary, Koh, Winston, additional, Seow, Yiqi, additional, Hoon, Shawn, additional, Venkatesh, Aparna, additional, Dashraath, Pradip, additional, Lim, Li Min, additional, Ong, Judith, additional, Lee, Rachel, additional, Johana, Nuryanti, additional, Yeo, Julie SL, additional, Chong, David, additional, Tan, Lay-Kok, additional, Chan, Jerry KY, additional, Choolani, Mahesh, additional, and Tambyah, Paul Anantharajah, additional

Published
2022
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10. Prevalence, risk factors and parental perceptions of gastroesophageal reflux disease in Asian infants in Singapore

Author

McLoughlin, Vanessa ZY, primary, Suaini, Noor HA, additional, Siah, Kewin, additional, Loo, Evelyn XL, additional, Pang, Wei Wei, additional, Chong, Yap Seng, additional, Godfrey, Keith M, additional, Tan, Kok Hian, additional, Chan, Jerry KY, additional, Goh, Anne EN, additional, Lee, Bee Wah, additional, Shek, Lynette P, additional, Eriksson, Johan G, additional, Aw, Marion M, additional, and Tham, Elizabeth H, additional

Published
2022
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11. Associations Between Maternal Distress During Early Life Periods and Offspring Respiratory Infections and Allergic Outcomes

Author

Lau, Hui Xing, Kee, Michelle Zhi Ling, Yap, Qai Ven, Tham, Elizabeth Huiwen, Chan, Yiong Huak, Goh, Anne Eng Neo, Teoh, Oon Hoe, Eriksson, Johan G., Godfrey, Keith, Gluckman, Peter D., Chong, Yap-Seng, Chan, Jerry KY, van Bever, Hugo, Lee, Bee Wah, Shek, Lynette Pei-Chi, Meaney, Michael J., Loo, Evelyn Xiu-Ling, Clinicum, Diabetes and Obesity Research Program, Johan Eriksson / Principal Investigator, Department of General Practice and Primary Health Care, and University of Helsinki

Subjects
RISK, postnatal, preconception, maternal distress, allergic sensitization, rhinitis, PRENATAL STRESS, PREGNANCY, 3123 Gynaecology and paediatrics, wheeze, Pediatrics, Perinatology and Child Health, ATOPIC-DERMATITIS, ASTHMA, ANXIETY, CHILDHOOD ECZEMA, eczema, PITUITARY-ADRENAL AXIS, PSYCHOLOGICAL DISTRESS, MULTIDIMENSIONAL SCALE
Abstract

BackgroundIncreasing evidence suggests that maternal distress is a risk factor for development of respiratory infections and allergic diseases in the offspring. We aim to evaluate the link between maternal distress during critical periods in early life, namely the preconception, pregnancy and postnatal periods, and development of respiratory infections and allergic diseases in the offspring from the Singapore PREconception Study of long Term maternal and child Outcomes (S-PRESTO) cohort.MethodsMaternal perceived distress was evaluated using validated questionnaires including Beck Depression Inventory-II (BDI-II) administered during three time periods: preconception (three months apart at four timepoints), pregnancy (during each trimester) and postnatal (3 and 6 months post-delivery). Child eczema, rhinitis and wheeze outcomes were evaluated using a modified ISAAC questionnaire at ages 3, 6, 12, and 18 months. Child allergic sensitization was determined by skin prick testing at 18 months.ResultsAmong 332 mother-child pairs studied, higher maternal distress during preconception and pregnancy increased the risks of wheeze development in the first 18 months; for example, preconception and pregnancy BDI-II scores ≥20 were associated with increased risks of wheeze by 18 months [adjusted risk ratios 3.2 (95%CI 1.1–9.4) and 2.5 (1.0–5.9), respectively]. Emotional and practical support from family during preconception decreased the risks of offspring wheeze. No associations were observed between maternal distress and offspring eczema, rhinitis and allergic sensitization.ConclusionMaternal distress during critical early life periods was associated with offspring wheeze in the first 18 months of life. Supporting maternal mental health even before pregnancy could reduce the risk of offspring wheeze.

Published
2022

12. Association of assisted reproductive technology with long-term offspring cardiometabolic health: a multi-cohort study

Author

Elhakeem, Ahmed, primary, Taylor, Amy E, additional, Inskip, Hazel M, additional, Huang, Jonathan, additional, Mansell, Toby, additional, Rodrigues, Carina, additional, Asta, Federica, additional, Blaauwendraad, Sophie M, additional, Haberg, Siri E, additional, Halliday, Jane, additional, Harskamp-van Ginkel, Margreet W, additional, He, Jian-Rong, additional, Jaddoe, Vincent WV, additional, Lewis, Sharon, additional, Maher, Gillian M, additional, Manios, Yannis, additional, McCarthy, Fergus P, additional, Reiss, Irwin KM, additional, Rusconi, Franca, additional, Salika, Theodosia, additional, Tafflet, Muriel, additional, Qiu, Xiu, additional, Asvold, Bjorn O, additional, Burgner, David, additional, Chan, Jerry KY, additional, Gagliardi, Luigi, additional, Gaillard, Romy, additional, Heude, Barbara, additional, Magnus, Maria C, additional, Moschonis, George, additional, Murray, Deirdre, additional, Nelson, Scott M, additional, Porta, Daniela, additional, Saffery, Richard, additional, Barros, Henrique, additional, Eriksson, Johan G, additional, Vrijkotte, Tanja GM, additional, and Lawlor, Deborah A, additional

Published
2022
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14. Identification and reproducibility of dietary patterns assessed with a FFQ among women planning pregnancy

Author

Lim, Shan Xuan, primary, Colega, Marjorelee T, additional, M Ayob, M Na’im, additional, Robinson, Sian M, additional, Godfrey, Keith M, additional, Bernard, Jonathan Y, additional, Lee, Yung Seng, additional, Tan, Kok Hian, additional, Yap, Fabian, additional, Shek, Lynette PC, additional, Chong, Yap Seng, additional, Eriksson, Johan G, additional, Chan, Jerry KY, additional, Chan, Shiao Yng, additional, and Chong, Mary FF, additional

Published
2021
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15. Pregnancy Outcomes in COVID-19: A Prospective Cohort Study in Singapore

Author

Mattar, Citra NZ, primary, Kalimuddin, Shirin, additional, Sadarangani, Sapna P, additional, Tagore, Shephali, additional, Thain, Serene, additional, Thoon, Koh Cheng, additional, Hong, Eliane Y, additional, Kanneganti, Abhiram, additional, Ku, Chee Wai, additional, Chan, Grace MF, additional, Lee, Kelvin ZX, additional, Yap, Jeannie JY, additional, Tan, Shaun S, additional, Yan, Benedict, additional, Young, Barnaby E, additional, Lye, David C, additional, Anderson, Danielle E, additional, Yang, Liying, additional, Su, Lin Lin, additional, Somani, Jyoti, additional, Tan, Lay-Kok, additional, Choolani, Mahesh A, additional, and Chan, Jerry KY, additional

Published
2020
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20. scRNA-seq reveals ACE2 and TMPRSS2 expression in TROP2+ Liver Progenitor Cells: Implications in COVID-19 associated Liver Dysfunction

Author

Wen Seow, Justine Jia, primary, Pai, Rhea, additional, Mishra, Archita, additional, Shepherdson, Edwin, additional, Hon Lim, Tony Kiat, additional, Goh, Brian KP, additional, Chan, Jerry KY, additional, Chow, Pierce KH, additional, Ginhoux, Florent, additional, DasGupta, Ramanuj, additional, and Sharma, Ankur, additional

Published
2020
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21. Immunological observations and transcriptomic analysis of trimester‐specific full‐term placentas from three Zika virus‐infected women

Author

Lum, Fok‐Moon, primary, Narang, Vipin, additional, Hue, Susan, additional, Chen, Jie, additional, McGovern, Naomi, additional, Rajarethinam, Ravisankar, additional, Tan, Jeslin JL, additional, Amrun, Siti Naqiah, additional, Chan, Yi‐Hao, additional, Lee, Cheryl YP, additional, Chua, Tze‐Kwang, additional, Yee, Wearn‐Xin, additional, Yeo, Nicholas KW, additional, Tan, Thiam‐Chye, additional, Liu, Xuan, additional, Haldenby, Sam, additional, Leo, Yee‐sin, additional, Ginhoux, Florent, additional, Chan, Jerry KY, additional, Hiscox, Julian, additional, Chong, Chia‐Yin, additional, and Ng, Lisa FP, additional

Published
2019
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23. Ovarian biomarkers predict controlled ovarian stimulation for in vitro fertilisation treatment in Singapore.

Author

Lee, Ryan Wai Kheong, Khin, Lay Wai, Hendricks, Marianne Sybille, Tan, Heng Hao, Nadarajah, Sadhana, Tee, Nancy Wen Sim, Loh, Seong-Feei, Tai, Bee Choo, and Chan, Jerry Ky

Subjects
OVARIES, INDUCED ovulation, FERTILIZATION in vitro
Abstract

Introduction: Ovarian biomarkers have been shown to predict responses to controlled ovarian hyperstimulation (COH) during in vitro fertilisation (IVF) in predominantly Caucasian populations, with limited studies performed in Southeast Asian women in Singapore.Methods: We evaluated the performance of serum anti-Müllerian hormone (AMH), follicle-stimulating hormone and oestradiol levels, antral follicle count (AFC), body mass index, ovarian volume, and age to establish thresholds for the prediction of poor (< 4 oocytes retrieved) and excessive responses (> 19 oocytes retrieved) in 263 women undergoing COH. Univariate and multivariate logistic regression analysis and receiver operating characteristic curves were used to calculate probabilities for poor and excessive responders to COH.Results: 36 (13.7%) and 50 (19.0%) women had poor and excessive response to COH, respectively. An AMH value of 0.69 ng/mL predicted poor ovarian response with positive likelihood ratio (LR) of 2.94, compared to an AFC of ≤ 5 when the positive LR is 2.36. Conversely, an AMH value of ≥ 3.06 ng/mL predicted excessive ovarian response with positive LR of 2.24, compared to an AFC cut-off of ≥ 12 with positive LR of 1.93.Conclusion: AMH levels and AFC are equivalent in the prediction of both poor and excessive ovarian response in women undergoing IVF. Our study highlights the importance of establishing population-specific cut-off biomarker values so that protocols can be tailored to optimise IVF treatment. [ABSTRACT FROM AUTHOR]

Published
2020
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24. Long-Term Fate of Human Fetal Liver Progenitor Cells Transplanted in Injured Mouse Livers

Author

Irudayaswamy, Antony, primary, Muthiah, Mark, additional, Zhou, Lei, additional, Hung, Hau, additional, Jumat, Nur Halisah Bte, additional, Haque, Jamil, additional, Teoh, Narcissus, additional, Farrell, Geoffrey, additional, Riehle, Kimberly J., additional, Lin, Jaymie Siqi, additional, Su, Lin Lin, additional, Chan, Jerry Ky, additional, Choolani, Mahesh, additional, Wong, Peng Cheang, additional, Wee, Aileen, additional, Lim, Seng Gee, additional, Campbell, Jean, additional, Fausto, Nelson, additional, and Dan, Yock Young, additional

Published
2017
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27. Zika Virus: An Evolving Public Health Threat

Author

Yung, Chee Fu, primary, Chong, Chia Yin, additional, Yeo, Kee Thai, additional, Liew, Christina, additional, Ng, Lee Ching, additional, Tan, Natalie WH, additional, Yeo, George SH, additional, Tee, Nancy WS, additional, Lin, Raymond TP, additional, Tan, Thiam Chye, additional, Rajadurai, Victor S, additional, Chan, Jerry KY, additional, and Thoon, Koh Cheng, additional

Published
2016
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28. Stem cell tracking with optically active nanoparticles

Author

Yu Gao, Cui, Yan, Chan, Jerry Ky, and Xu, Chenjie

Subjects
genetic structures, Review Article
Abstract

Stem-cell-based therapies hold promise and potential to address many unmet clinical needs. Cell tracking with modern imaging modalities offers insight into the underlying biological process of the stem-cell-based therapies, with the goal to reveal cell survival, migration, homing, engraftment, differentiation, and functions. Adaptability, sensitivity, resolution, and non-invasiveness have contributed to the longstanding use of optical imaging for stem cell tracking and analysis. To identify transplanted stem cells from the host tissue, optically active probes are usually used to label stem cells before the administration. In comparison to the traditional fluorescent probes like fluorescent proteins and dyes, nanoparticle-based probes are advantageous in terms of the photo-stabilities and minimal changes to the cell phenotype. The main focus here is to overview the recent development of optically active nanoparticles for stem cells tracking. The related optical imaging modalities include fluorescence imaging, photoacoustic imaging, Raman and surface enhanced Raman spectroscopy imaging.

Published
2013

29. Long-Term Fate of Human Fetal Liver Progenitor Cells Transplanted in Injured Mouse Livers.

Author

Irudayaswamy, Antony, Muthiah, Mark, Zhou, Lei, Hung, Hau, Jumat, Nur Halisah Bte, Haque, Jamil, Teoh, Narcissus, Farrell, Geoffrey, Riehle, Kimberly J., Lin, Jaymie Siqi, Su, Lin Lin, Chan, Jerry Ky, Choolani, Mahesh, Wong, Peng Cheang, Wee, Aileen, Lim, Seng Gee, Campbell, Jean, Fausto, Nelson, and Dan, Yock Young

Abstract

Liver progenitor cells have the potential to repair and regenerate a diseased liver. The success of any translational efforts, however, hinges on thorough understanding of the fate of these cells after transplant, especially in terms of long-term safety and efficacy. Here, we report transplantation of a liver progenitor population isolated from human fetal livers into immune-permissive mice with follow-up up to 36 weeks after transplant. We found that human progenitor cells engraft and differentiate into functional human hepatocytes in the mouse, producing albumin, alpha-1-antitrypsin, and glycogen. They create tight junctions with mouse hepatocytes, with no evidence of cell fusion. Interestingly, they also differentiate into functional endothelial cell and bile duct cells. Transplantation of progenitor cells abrogated carbon tetrachloride-induced fibrosis in recipient mice, with downregulation of procollagen and anti-smooth muscle actin. Paradoxically, the degree of engraftment of human hepatocytes correlated negatively with the anti-fibrotic effect. Progenitor cell expansion was most prominent in cirrhotic animals, and correlated with transcript levels of pro-fibrotic genes. Animals that had resolution of fibrosis had quiescent native progenitor cells in their livers. No evidence of neoplasia was observed, even up to 9 months after transplantation. Human fetal liver progenitor cells successfully attenuate liver fibrosis in mice. They are activated in the setting of liver injury, but become quiescent when injury resolves, mimicking the behavior of de novo progenitor cells. Our data suggest that liver progenitor cells transplanted into injured livers maintain a functional role in the repair and regeneration of the liver. S tem C ells 2018;36:103-113 [ABSTRACT FROM AUTHOR]

Published
2018
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34. Stable Human FIX Expression After 0.9G Intrauterine Gene Transfer of Self-complementary Adeno-associated Viral Vector 5 and 8 in Macaques

Author

Mattar, Citra NZ, primary, Nathwani, Amit C, additional, Waddington, Simon N, additional, Dighe, Niraja, additional, Kaeppel, Christine, additional, Nowrouzi, Ali, additional, Mcintosh, Jenny, additional, Johana, Nuryanti B, additional, Ogden, Bryan, additional, Fisk, Nicholas M, additional, Davidoff, Andrew M, additional, David, Anna, additional, Peebles, Donald, additional, Valentine, Marcus B, additional, Appelt, Jens-Uwe, additional, von Kalle, Christof, additional, Schmidt, Manfred, additional, Biswas, Arijit, additional, Choolani, Mahesh, additional, and Chan, Jerry KY, additional

Published
2011
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36. Mapping the human DC lineage through the integration of high-dimensional techniques

Author

See, Peter, Dutertre, Charles-Antoine, Chen, Jinmiao, Günther, Patrick, McGovern, Naomi, Irac, Sergio Erdal, Gunawan, Merry, Beyer, Marc, Händler, Kristian, Duan, Kaibo, Sumatoh, Hermi Rizal Bin, Ruffin, Nicolas, Jouve, Mabel, Gea-Mallorquí, Ester, Hennekam, Raoul CM, Lim, Tony, Yip, Chan Chung, Wen, Ming, Malleret, Benoit, Low, Ivy, Shadan, Nurhidaya Binte, Fen, Charlene Foong Shu, Tay, Alicia, Lum, Josephine, Zolezzi, Francesca, Larbi, Anis, Poidinger, Michael, Chan, Jerry KY, Chen, Qingfeng, Rénia, Laurent, Haniffa, Muzlifah, Benaroch, Philippe, Schlitzer, Andreas, Schultze, Joachim L, Newell, Evan W, and Ginhoux, Florent

Subjects
Blood Cells, Sequence Analysis, RNA, Humans, Cell Differentiation, Cell Lineage, Dendritic Cells, Cell Separation, Single-Cell Analysis, 3. Good health, Unsupervised Machine Learning

37. Longitudinal characterization of determinants associated with obesogenic growth patterns in early childhood.

Author

Michael N, Gupta V, Fogel A, Huang J, Chen L, Sadananthan SA, Ong YY, Aris IM, Pang WW, Yuan WL, Loy SL, Thway Tint M, Tan KH, Chan JK, Chan SY, Shek LP, Yap F, Godfrey K, Chong YS, Gluckman P, Velan SS, Forde CG, Lee YS, Eriksson JG, and Karnani N

Subjects
Female, Child, Child, Preschool, Humans, Pregnancy, Prospective Studies, Body Mass Index, Risk Factors, Pediatric Obesity epidemiology, Cardiovascular Diseases
Abstract

Background: Longitudinal assessment of the determinants of obesogenic growth trajectories in childhood can suggest appropriate developmental windows for intervention., Methods: Latent class growth mixture modelling was used to identify body mass index (BMI) z-score trajectories from birth to age 6 years in 994 children from a prospective mother-offspring cohort (Chinese, Indian and Malay ethnicities) based in Singapore. We evaluated the early-life determinants of the trajectories as well as their associations with cardiometabolic risk markers at age 6 years., Results: Five BMI z-score trajectory patterns were identified, three within the healthy weight range, alongside early-acceleration and late-acceleration obesogenic trajectories. The early-acceleration pattern was characterized by elevated fetal abdominal circumference growth velocity, BMI acceleration immediately after birth and crossing of the obesity threshold by age 2 years. The late-acceleration pattern had normal fetal growth and BMI acceleration after infancy, and approached the obesity threshold by age 6 years. Abdominal fat, liver fat, insulin resistance and odds of pre-hypertension/hypertension were elevated in both groups. Indian ethnicity, high pre-pregnancy BMI, high polygenic risk scores for obesity and shorter breastfeeding duration were common risk factors for both groups. Malay ethnicity and low maternal educational attainment were uniquely associated with early BMI acceleration, whereas nulliparity and obesogenic eating behaviours in early childhood were uniquely associated with late BMI acceleration., Conclusion: BMI acceleration starting immediately after birth or after infancy were both linked to early cardiometabolic alterations. The determinants of these trajectories may be useful for developing early risk stratification and intervention approaches to counteract metabolic adversities linked to childhood obesity., (© The Author(s) 2022; all rights reserved. Published by Oxford University Press on behalf of the International Epidemiological Association.)

Published
2023
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38. Stem cell tracking with optically active nanoparticles.

Author

Gao Y, Cui Y, Chan JK, and Xu C

Abstract

Stem-cell-based therapies hold promise and potential to address many unmet clinical needs. Cell tracking with modern imaging modalities offers insight into the underlying biological process of the stem-cell-based therapies, with the goal to reveal cell survival, migration, homing, engraftment, differentiation, and functions. Adaptability, sensitivity, resolution, and non-invasiveness have contributed to the longstanding use of optical imaging for stem cell tracking and analysis. To identify transplanted stem cells from the host tissue, optically active probes are usually used to label stem cells before the administration. In comparison to the traditional fluorescent probes like fluorescent proteins and dyes, nanoparticle-based probes are advantageous in terms of the photo-stabilities and minimal changes to the cell phenotype. The main focus here is to overview the recent development of optically active nanoparticles for stem cells tracking. The related optical imaging modalities include fluorescence imaging, photoacoustic imaging, Raman and surface enhanced Raman spectroscopy imaging.

Published
2013

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