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14 results on '"Chang, Shuwei"'

8. Additional file 1 of Genome-wide identification and characterisation of bHLH transcription factors in Artemisia annua

Author

Chang, Shuwei, Li, Qi, Huang, Baokang, Chen, Wansheng, and Tan, Hexin

Abstract

Additional file 1: Table S1. All Gene ontology (GO) annotation of AabHLH TFs involved into. Table S2. All AabHLH TFs are divided into different categories. Fig. S1. Protein–protein interaction (PPI) network. Fig. S2. Expression of genes encoding total AabHLH TFs across A. annua tissues and stages of development. Table S3. qRT-PCR primers.

Published
2023
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9. OsFH3 Encodes a Type II Formin Required for Rice Morphogenesis

Author

Chang, Shuwei, primary, Ren, Zhanhong, additional, Liu, Chang, additional, Du, Pingzhou, additional, Li, Jingbin, additional, Liu, Zengyu, additional, Zhang, Fengli, additional, Hou, Haili, additional, Shi, Jianxin, additional, Liang, Wanqi, additional, Yang, Litao, additional, Ren, Haiyun, additional, and Zhang, Dabing, additional

Published
2021
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14. Generation of an Efficient Artificial Promoter of Bovine Skeletal Muscle Α-Actin Gene (Acta1) Through Addition of Cis-Acting Element.

Author

Hu, Qian, Tong, Huili, Zhao, Dandan, Cao, Yunkao, Zhang, Weiwei, Chang, Shuwei, Yang, Yu, and Yan, Yunqin

Abstract

The promoter of skeletal muscle α-actin gene (ACTA1) is highly muscle specific. The core of the bovine ACTA1 promoter extends from +29 to −233, about 262 base pairs (bp), which is sufficient to activate transcription in bovine muscle satellite cells. In this study, analysis by PCR site-specific mutagenesis showed that the cis-acting element SRE (serum response element binding factor) was processed as a transcriptional activator. In order to enhance the bovine ACTA1 promoter's activity, we used a strategy to modify it. We cloned a fragment containing three SREs from the promoter of ACTA1, and then one or two clones were linked upstream of the core promoter (262 bp) of ACTA1. One and two clones increased the activity of the ACTA1 promoter 3-fold and 10-fold, respectively, and maintained muscle tissue specificity. The modified promoter with two clones could increase the level of ACTA1 mRNA and protein 4-fold and 1.1-fold, respectively. Immunofluorescence results showed that green fluorescence of ACTA1 increased. Additionally, the number of total muscle microfilaments increased. These genetically engineered promoters might be useful for regulating gene expression in muscle cells and improving muscle mass in livestock. [ABSTRACT FROM AUTHOR]

Published
2015
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