1. Molecular characterization of a CXCL8-like protein from ayu and its effect on chemotaxis of neutrophils and monocytes/macrophages
- Author
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Chang-Hong Li, Xin-Jiang Lu, Jiong Chen, and Chang-Qing Chu
- Subjects
Fish Proteins ,Chemokine ,Neutrophils ,Molecular Sequence Data ,Inflammation ,Listonella anguillarum ,Monocytes ,law.invention ,Microbiology ,Fish Diseases ,Immune system ,law ,Genetics ,medicine ,Animals ,Interleukin 8 ,Amino Acid Sequence ,Cloning, Molecular ,Cells, Cultured ,Phylogeny ,biology ,Chemotaxis ,Macrophages ,Interleukin-8 ,General Medicine ,biology.organism_classification ,Molecular biology ,Gene Expression Regulation ,Osmeriformes ,Host-Pathogen Interactions ,biology.protein ,Recombinant DNA ,medicine.symptom ,Gram-Negative Bacterial Infections ,Listonella ,Chemotaxis assay - Abstract
CXCL8, a CXC-type chemokine, plays a crucial role in acute inflammation by recruiting and mediating neutrophils and other cells. In this study, the cDNA and genomic DNA sequence of a CXCL8-like protein (PaCXCL8l) from ayu ( Plecoglossus altivelis ) was determined. Sequence analysis showed that PaCXCL8l represented the typical structure of animal CXCL8s. Phylogenetic tree analysis indicated that PaCXCL8l was closest to CXCL8 of Atlantic cod ( Gadus morhua ). Constitutive expression of PaCXCL8l was detected in all tested tissues and monocytes/macrophages, and its expression dramatically increased upon Listonella anguillarum infection. In vitro, recombinant PaCXCL8l exhibited a significant chemotactic effect on neutrophils at 0.1 μg/ml and on monocytes/macrophages at 1.0 μg/ml. In vivo, the numbers of peritoneal neutrophils and monocytes/macrophages were both up-regulated following intraperitoneal administration of recombinant PaCXCL8l. These results suggest that PaCXCL8l is crucially involved in the immune response of ayu by mediating chemotaxis of neutrophils and monocytes/macrophages.
- Published
- 2014