1. Exploring the microbiota difference of bronchoalveolar lavage fluid between community-acquired pneumonia with or without COPD based on metagenomic sequencing: a retrospective study
- Author
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Bingbing Wang, Min Tan, Wei Li, Qinghua Xu, Lianfeng Jin, Shuanshuan Xie, and Changhui Wang
- Subjects
Community acquired pneumonia ,Chronic obstructive pulmonary disease ,Bronchoalveolar lavage fluid ,Metagenomic next-generation sequencing ,Diseases of the respiratory system ,RC705-779 - Abstract
Abstract Background Community-acquired pneumonia (CAP) patients with chronic obstructive pulmonary disease (COPD) have higher disease severity and mortality compared to those without COPD. However, deep investigation into microbiome distribution of lower respiratory tract of CAP with or without COPD was unknown. Methods So we used metagenomic next generation sequencing (mNGS) to explore the microbiome differences between the two groups. Results Thirty-six CAP without COPD and 11 CAP with COPD cases were retrieved. Bronchoalveolar lavage fluid (BALF) was collected and analyzed using untargeted mNGS and bioinformatic analysis. mNGS revealed that CAP with COPD group was abundant with Streptococcus, Prevotella, Bordetella at genus level and Cutibacterium acnes, Rothia mucilaginosa, Bordetella genomosp. 6 at species level. While CAP without COPD group was abundant with Ralstonia, Prevotella, Streptococcus at genus level and Ralstonia pickettii, Rothia mucilaginosa, Prevotella melaninogenica at species level. Meanwhile, both alpha and beta microbiome diversity was similar between groups. Linear discriminant analysis found that pa-raburkholderia, corynebacterium tuberculostearicum and staphylococcus hominis were more enriched in CAP without COPD group while the abundance of streptococcus intermedius, streptococcus constellatus, streptococcus milleri, fusarium was higher in CAP with COPD group. Conclusions These findings revealed that concomitant COPD have an mild impact on lower airway microbiome of CAP patients.
- Published
- 2024
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