7 results on '"Changjie Ouyang"'
Search Results
2. Short- and long-term effects of 3.5-23.0 Tesla ultra-high magnetic fields on mice behaviour
- Author
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Md Hasanuzzaman Khan, Xinfeng Huang, Xiaofei Tian, Changjie Ouyang, Dongmei Wang, Shuang Feng, Jutao Chen, Tian Xue, Jin Bao, and Xin Zhang
- Subjects
Mice ,Cognition ,Magnetic Fields ,Animals ,Radiology, Nuclear Medicine and imaging ,General Medicine ,Magnetic Resonance Imaging - Abstract
Higher static magnetic field (SMF) enables higher imaging capability in magnetic resonance imaging (MRI), which encourages the development of ultra-high field MRIs above 20 T with a prerequisite for safety issues. However, animal tests of ≥ 20 T SMF exposure are very limited. The objective of the current study is to evaluate mice behaviour consequences of 3.5-23.0 T SMF exposure.We systematically examined 112 mice for their short- and long-term behaviour responses to a 2-h exposure of 3.5-23.0 T SMFs. Locomotor activity and cognitive functions were measured by five behaviour tests, including balance beam, open field, elevated plus maze, three-chamber social recognition, and Morris water maze tests.Besides the transient short-term impairment of the sense of balance and locomotor activity, the 3.5-23.0 T SMFs did not have long-term negative effects on mice locomotion, anxiety level, social behaviour, or memory. In contrast, we observed anxiolytic effects and positive effects on social and spatial memory of SMFs, which is likely correlated with the significantly increased CaMKII level in the hippocampus region of high SMF-treated mice.Our study showed that the short exposures to high-field SMFs up to 23.0 T have negligible side effects on healthy mice and may even have beneficial outcomes in mice mood and memory, which is pertinent to the future medical application of ultra-high field SMFs in MRIs and beyond.• Short-term exposure to magnetic fields up to 23.0 T is safe for mice. • High-field static magnetic field exposure transiently reduced mice locomotion. • High-field static magnetic field enhances memory while reduces the anxiety level.
- Published
- 2021
3. Melanopsin retinal ganglion cells mediate light-promoted brain development
- Author
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Jiaxi Hu, Yiming Shi, Jiaming Zhang, Xinfeng Huang, Qian Wang, Hang Zhao, Jiawei Shen, Zhiping Chen, Wei Song, Ping Zheng, Shulu Zhan, Yanping Sun, Pengfei Cai, Kai An, Changjie Ouyang, Baizhen Zhao, Qixin Zhou, Lin Xu, Wei Xiong, Zhi Zhang, Jianjun Meng, Jutao Chen, Yuqian Ma, Huan Zhao, Mei Zhang, Kun Qu, Ji Hu, Minhua Luo, Fuqiang Xu, Xiaowei Chen, Ying Xiong, Jin Bao, and Tian Xue
- Subjects
Retinal Ganglion Cells ,Mice ,Rod Opsins ,Animals ,Brain ,Humans ,Oxytocin ,General Biochemistry, Genetics and Molecular Biology - Abstract
During development, melanopsin-expressing intrinsically photosensitive retinal ganglion cells (ipRGCs) become light sensitive much earlier than rods and cones. IpRGCs project to many subcortical areas, whereas physiological functions of these projections are yet to be fully elucidated. Here, we found that ipRGC-mediated light sensation promotes synaptogenesis of pyramidal neurons in various cortices and the hippocampus. This phenomenon depends on activation of ipRGCs and is mediated by the release of oxytocin from the supraoptic nucleus (SON) and the paraventricular nucleus (PVN) into cerebral-spinal fluid. We further characterized a direct connection between ipRGCs and oxytocin neurons in the SON and mutual projections between oxytocin neurons in the SON and PVN. Moreover, we showed that the lack of ipRGC-mediated, light-promoted early cortical synaptogenesis compromised learning ability in adult mice. Our results highlight the importance of light sensation early in life on the development of learning ability and therefore call attention to suitable light environment for infant care.
- Published
- 2021
4. Guiding role of seminal tract anatomical study in transurethral seminal vesiculoscopy
- Author
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Longjun Cai, Wen-da Zhang, Conghui Han, Dewei Qu, Jianjun Zhang, Bo Jiang, Wen Yang, Changjie Ouyang, Bo Chen, Kun Pang, Deguang Wang, Zhenduo Shi, Lin Hao, and Zhiguo Zhang
- Subjects
seminal tract anatomical observation ,complications ,Urology ,transurethral seminal vesiculoscopy ,030232 urology & nephrology ,03 medical and health sciences ,0302 clinical medicine ,surgical effect ,Cadaver ,medicine ,Seminal tract ,Pelvis ,Original Paper ,ejaculatory duct and seminal vesicle diseases ,business.industry ,Gastroenterology ,Obstetrics and Gynecology ,Anatomy ,Clinical Practice ,medicine.anatomical_structure ,030220 oncology & carcinogenesis ,Medicine ,Surgery ,Cadaveric spasm ,business ,Duct (anatomy) - Abstract
Introduction There have been problems with low qualification operator-related complications and failures of transurethral seminal vesiculoscopy (TSV) in China. Aim To study the guiding role of seminal tract anatomical study (STAS) in TSV. Material and methods We performed STAS to study the structure, morphology, duct trajectory, and anatomical relationships between the seminal vesicles and the adjacent tissue in pelvic specimens from 12 adult cadavers. Then the surgical effects and complications of 82 cases of TSV performed by 3 doctors were retrospectively studied to compare the difference between the two groups of before and after the anatomical study. Results The anatomical studies of the 12 adult cadaveric pelvis specimens identified the lengths and widths of the right- and left-side seminal vesicles and tracts. The TSV can treat lesions located in the distal seminal tract and vesicle, but proximal lesions cannot be reached, which is an anatomical limitation of this technique. There were significant differences in the surgical times and the surgical validity rates between the 2 groups. Conclusions Our anatomical study of the seminal tract and seminal vesicles is valuable for guiding TSV in clinical practice.
- Published
- 2019
5. HO-1 attenuates hippocampal neurons injury via the activation of BDNF–TrkB–PI3K/Akt signaling pathway in stroke
- Author
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Weihao Zhang, Yu-Lan Wang, Tengteng Li, Lihua Yang, Yuan-Jian Song, Dashi Qi, Changjie Ouyang, Lei Sheng, Hongli Yu, Jiali Tang, Wei Fu, Mei Wang, Xijuan Ma, Xiao-Jing Huang, Hongyan Dong, Lei Miao, and Shi-Chun Zhang
- Subjects
Male ,medicine.medical_specialty ,medicine.drug_class ,Carbazoles ,Ischemia ,Apoptosis ,Tropomyosin receptor kinase B ,Hippocampal formation ,Transfection ,Neuroprotection ,Brain Ischemia ,Indole Alkaloids ,Rats, Sprague-Dawley ,Phosphatidylinositol 3-Kinases ,chemistry.chemical_compound ,Internal medicine ,medicine ,Animals ,Receptor, trkB ,Enzyme Inhibitors ,CA1 Region, Hippocampal ,Molecular Biology ,Neurons ,Caspase 3 ,business.industry ,Akt/PKB signaling pathway ,Brain-Derived Neurotrophic Factor ,General Neuroscience ,medicine.disease ,Receptor antagonist ,Stroke ,Disease Models, Animal ,Endocrinology ,nervous system ,chemistry ,Heme Oxygenase (Decyclizing) ,Neurology (clinical) ,K252a ,business ,Proto-Oncogene Proteins c-akt ,Neuroscience ,Signal Transduction ,Developmental Biology - Abstract
Although recent studies have found that HO-1 plays an important role in neuronal survival, little is known about the precise mechanisms occurring during cerebral ischemia/reperfusion (I/R). Therefore, the aim of this study was to investigate the neuroprotective mechanisms of HO-1 against ischemic brain injury induced by cerebral I/R and to explore whether the BDNF-TrkB-PI3K/Akt signaling pathway contributed to the protection provided by HO-1. Over-expressed HO-1 plasmids were employed to induce the overexpression of HO-1 through hippocampi CA1 injection 5 days before the cerebral I/R animal model was induced by four-vessel occlusion for 15 min transient ischemia and followed by reperfusion in Sprague-Dawley rats. Immunoblotting was carried out to examine the expression of the related proteins, and HE-staining was used to detect the percentage of living neurons in the hippocampal CA1 region. The results showed that over-expressed HO-1 could significantly protect neurons against cerebral I/R. Furthermore, the protein expression of BDNF, TrkB and p-Akt also increased in the rats treated with over-expressed HO-1 plasmids. However, treatment with tropomyosin receptor kinase B (TrkB) receptor antagonist (K252a) reversed the HO-1-induced increase in BDNF and p-Akt protein levels and decreased the level of cleaved caspase-3 protein in I/R rats. In summary, our results imply that HO-1 can decrease cell apoptosis in the I/R rat brain and that the mechanism may be related to the activation of the BDNF-TrkB-PI3K/Akt signaling pathway.
- Published
- 2014
6. Guiding role of seminal tract anatomical study in transurethral seminal vesiculoscopy.
- Author
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Kun Pang, Wen Yang, Jianjun Zhang, Longjun Cai, Bo Chen, Zhiguo Zhang, Lin Hao, Zhenduo Shi, Bo Jiang, Changjie Ouyang, Dewei Qu, Deguang Wang, Conghui Han, and Wenda Zhang
- Subjects
SEMINAL vesicles ,MALE reproductive organs ,SURGICAL complications - Abstract
Introduction: There have been problems with low qualification operator-related complications and failures of transurethral seminal vesiculoscopy (TSV) in China. Aim: To study the guiding role of seminal tract anatomical study (STAS) in TSV. Material and methods: We performed STAS to study the structure, morphology, duct trajectory, and anatomical relationships between the seminal vesicles and the adjacent tissue in pelvic specimens from 12 adult cadavers. Then the surgical effects and complications of 82 cases of TSV performed by 3 doctors were retrospectively studied to compare the difference between the two groups of before and after the anatomical study. Results: The anatomical studies of the 12 adult cadaveric pelvis specimens identified the lengths and widths of the right- and left-side seminal vesicles and tracts. The TSV can treat lesions located in the distal seminal tract and vesicle, but proximal lesions cannot be reached, which is an anatomical limitation of this technique. There were significant differences in the surgical times and the surgical validity rates between the 2 groups. Conclusions: Our anatomical study of the seminal tract and seminal vesicles is valuable for guiding TSV in clinical practice. [ABSTRACT FROM AUTHOR]
- Published
- 2020
- Full Text
- View/download PDF
7. HO-1 attenuates hippocampal neurons injury via the activation of BDNF-TrkB-PI3K/Akt signaling pathway in stroke.
- Author
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Dashi Qi, Changjie Ouyang, Yulan Wang, Shichun Zhang, Xijuan Ma, YuanJian Song, HongLi Yu, Jiali Tang, Wei Fu, Lei Sheng, Lihua Yang, Mei Wang, Weihao Zhang, Lei Miao, Tengteng Li, Xiaojing Huang, and Hongyan Dong
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HIPPOCAMPUS (Brain) , *BRAIN-derived neurotrophic factor , *CELLULAR signal transduction , *CEREBRAL ischemia , *HEME oxygenase , *NEUROPROTECTIVE agents - Abstract
Although recent studies have found that HO-1 plays an important role in neuronal survival, little is known about the precise mechanisms occurring during cerebral ischemia/reperfusion (I/R). Therefore, the aim of this study was to investigate the neuroprotective mechanisms of HO-1 against ischemic brain injury induced by cerebral I/R and to explore whether the BDNF-TrkB-PI3K/Akt signaling pathway contributed to the protection provided by HO-1. Over-expressed HO-1 plasmids were employed to induce the overexpression of HO-1 through hippocampi CA1 injection 5 days before the cerebral I/R animal model was induced by four-vessel occlusion for 15 min transient ischemia and followed by reperfusion in Sprague-Dawley rats. Immunoblotting was carried out to examine the expression of the related proteins, and HE-staining was used to detect the percentage of living neurons in the hippocampal CA1 region. The results showed that over-expressed HO-1 could significantly protect neurons against cerebral I/R. Furthermore, the protein expression of BDNF, TrkB and p-Akt also increased in the rats treated with over-expressed HO-1 plasmids. However, treatment with tropomyosin receptor kinase B (TrkB) receptor antagonist (K252a) reversed the HO-1-induced increase in BDNF and p-Akt protein levels and decreased the level of cleaved caspase-3 protein in I/R rats. In summary, our results imply that HO-1 can decrease cell apoptosis in the I/R rat brain and that the mechanism may be related to the activation of the BDNF-TrkB-PI3K/Akt signaling pathway. [ABSTRACT FROM AUTHOR]
- Published
- 2014
- Full Text
- View/download PDF
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