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In this research, animal samples were subjected to skin rejuvenation using the floating electrode dielectric barrier discharge plasma device. Plasma processing was performed on the dorsal area of the rat's neck, covering a surface area of 18 cm2. Biometric assessments, encompassing measurements of skin surface evaporation, moisture content, melanin and erythema levels, as well as skin firmness and elasticity, were conducted before treatment initiation, immediately posttreatment, at the 4th and the 10th week during the follow‐up period. Physical examinations were employed to investigate and record the active species generated by plasma as well as the temperature of the skin surface. The skin ultrasound device was utilized to assess the density and thickness of both the epidermis and dermis. [ABSTRACT FROM AUTHOR]

This study used the FEDBD plasma device for skin rejuvenation in animal samples. There were two groups of six male Wistar rats. Before starting the treatment, immediately after the treatment, the fourth week, and the tenth week of follow-up, biometric tests were performed, including moisture level, evaporation from the skin surface, erythema and melanin, skin elasticity and firmness with an MPA9 device and cutometer. The thickness and density of the epidermis and dermis, an essential indicator in rejuvenation, were evaluated with a skin ultrasound device. Also, the level of oxygen, perfusion, and interstitial water (edema) was checked using a Tivita tissue hyperspectral camera at a depth of 6 mm of the skin., Competing Interests: The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (© 2023 The Authors. Published by Elsevier Ltd.)