Search

Your search keyword '"Charlebois D"' showing total 78 results
Start Over Author "Charlebois D"
78 results on '"Charlebois D"'

1. Advancing Drug Resistance Research Through Quantitative Modeling and Synthetic Biology

Author

Farquhar, K., Flohr, H., and Charlebois, D. A.

Subjects
Quantitative Biology - Quantitative Methods, Physics - Biological Physics, Quantitative Biology - Molecular Networks
Abstract

Antimicrobial resistance is an emerging global health crisis that is undermining advances in modern medicine and, if unmitigated, threatens to kill 10 million people per year worldwide by 2050. Research over the last decade has demonstrated that the differences between genetically identical cells in the same environment can lead to drug resistance. Fluctuations in gene expression, modulated by gene regulatory networks, can lead to non-genetic heterogeneity that results in the fractional killing of microbial populations causing drug therapies to fail; this non-genetic drug resistance can enhance the probability of acquiring genetic drug resistance mutations. Mathematical models of gene networks can elucidate general principles underlying drug resistance, predict the evolution of resistance, and guide drug resistance experiments in the laboratory. Cells genetically engineered to carry synthetic gene networks regulating drug resistance genes allow for controlled, quantitative experiments on the role of non-genetic heterogeneity in the development of drug resistance. In this perspective article, we emphasize the contributions that mathematical, computational, and synthetic gene network models play in advancing our understanding of antimicrobial resistance to discover effective therapies against drug-resistant infections., Comment: 16 pages, 2 figures

Published
2020
Full Text
View/download PDF

4. Nursing management

Author

Burns, S.M., primary, Charlebois, D., additional, Deivert, M., additional, Krenitsky, J., additional, and Wilmoth, D., additional

Published
2004
Full Text
View/download PDF

10. Three territorial approaches and three cases, in Argentina, in Canada and in France : Territoriesnet.org in motion

Author

Bozzano , Horacio, Charlebois , D, Gagnon , S, Signoret , Philippe, Universidad Nacional de la Plata [Argentine] (UNLP), Théoriser et modéliser pour aménager (UMR 6049) (ThéMA), Université de Bourgogne (UB)-Centre National de la Recherche Scientifique (CNRS)-Université de Franche-Comté (UFC), Université Bourgogne Franche-Comté [COMUE] (UBFC)-Université Bourgogne Franche-Comté [COMUE] (UBFC), Centre National de la Recherche Scientifique (CNRS)-Université de Bourgogne (UB)-Université de Franche-Comté (UFC), Universidad Nacional de la Plata ( UNLP ), Théoriser et modéliser pour aménager ( ThéMA ), Université de Bourgogne ( UB ) -Centre National de la Recherche Scientifique ( CNRS ) -Université de Franche-Comté ( UFC ), and Clauzel, Céline

Subjects
[SHS.GEO] Humanities and Social Sciences/Geography, [SHS.GEO]Humanities and Social Sciences/Geography, ComputingMilieux_MISCELLANEOUS, [ SHS.GEO ] Humanities and Social Sciences/Geography
Abstract

International audience

Published
2009

24. Early recognition of pulmonary embolism: the key to lowering mortality.

Author

Charlebois D

Abstract

Pulmonary embolism is a major cause of morbidity and mortality in the United States. The majority of deaths from pulmonary embolism occur because an accurate diagnosis was not made. It is imperative for clinicians to have a high level of clinical suspicion of pulmonary embolism when patients present with dyspnea, tachypnea, chest pain, hemoptysis, and cough. If pulmonary embolism is diagnosed and treatment initiated, death and recurrence of embolism are uncommon. Beyond correct diagnosis and treatment, the single most effective strategy that can be employed to decrease the high mortality associated with pulmonary embolism is identification of individuals at risk and the institution of prophylactic measures. This article reviews the incidence, risk factors, assessment, physical examination, laboratory, and diagnostic testing for pulmonary embolism. [ABSTRACT FROM AUTHOR]

Published
2005
Full Text
View/download PDF

50. Sample-to-answer centrifugal microfluidic droplet PCR platform for quantitation of viral load.

Author

Malic L, Clime L, Moon BU, Nassif C, Da Fonte D, Brassard D, Lukic L, Geissler M, Morton K, Charlebois D, and Veres T

Subjects
Humans, Lab-On-A-Chip Devices, Microfluidic Analytical Techniques instrumentation, COVID-19 diagnosis, COVID-19 virology, Polymerase Chain Reaction instrumentation, Equipment Design, Viral Load instrumentation, SARS-CoV-2 isolation & purification, SARS-CoV-2 genetics, Centrifugation instrumentation, RNA, Viral analysis, RNA, Viral isolation & purification, RNA, Viral genetics
Abstract

Droplet digital polymerase chain reaction (ddPCR) stands out as a highly sensitive diagnostic technique that is gaining traction in infectious disease diagnostics due to its ability to quantitate very low numbers of viral gene copies. By partitioning the sample into thousands of droplets, ddPCR enables precise and absolute quantification without relying on a standard curve. However, current ddPCR systems often exhibit relatively low levels of integration, and the analytical process remains dependent on elaborate workflows for up-front sample preparation. Here, we introduce a fully-integrated system seamlessly combining viral lysis, RNA extraction, emulsification, reverse transcription (RT) ddPCR, and fluorescence readout in a sample-to-answer format. The system comprises a disposable microfluidic cartridge housing buffers and reagents required for the assay, and a centrifugal platform that allows for pneumatic actuation of liquids during rotation, enabling automation of the workflow. Highly monodisperse droplets (∼50 μm in diameter) are produced using centrifugal step emulsification and automatically transferred to an integrated heating module for target amplification. The platform is equipped with a miniature fluorescence imaging system enabling on-chip read-out of droplets after RT-ddPCR. We demonstrate sample-to-answer detection of SARS-CoV-2 N and E genes, along with RNase P endogenous reference, using hydrolysis probes and multiplexed amplification within single droplets for concentrations as low as 0.1 copy per μL. We also tested 14 nasopharyngeal swab specimens from patients and were able to distinguish positive and negative SARS-CoV-2 samples with 100% accuracy, surpassing results obtained by conventional real-time amplification.

Published
2024
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results