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5. The TaCslA12 gene expressed in the wheat grain endosperm synthesizes wheat-like mannan when expressed in yeast and Arabidopsis

Author

Verhertbruggen, Y., Bouder, A., Vigouroux, J., Alvarado, C., Geairon, A., Guillon, F., Wilkinson, M. D., Stritt, F., Pauly, M., Lee, M. Y., Mortimer, J. C., Scheller, H. V., Mitchell, R. A. C., Voiniciuc, C., Saulnier, L., Chateigner-Boutin, A.-L.., Unité de recherche sur les Biopolymères, Interactions Assemblages (BIA), Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Rothamsted Research, Biotechnology and Biological Sciences Research Council (BBSRC), Heinrich Heine Universität Düsseldorf = Heinrich Heine University [Düsseldorf], Lawrence Berkeley Natl Lab, Joint BioEnergy Inst, Feedstocks Div, University of California [Berkeley] (UC Berkeley), University of California (UC), Leibniz Institute of Plant Biochemistry (IPB), German Research Foundation (DFG) : EXC 2048/1, PA 1366/1-1, 414353267 (with European Commission). United States Department of Energy (DOE) : DE-AC02-05CH11231., ANR-11-INBS-0012,PHENOME,Centre français de phénomique végétale(2011), European Project, University of California [Berkeley], and University of California

Subjects
Crop and Pasture Production, Plant Biology & Botany, Arabidopsis, Plant Biology, Genetically Modified, chemical and pharmacologic phenomena, Saccharomyces cerevisiae, Genes, Plant, Mannans, [SDV.GEN.GPL]Life Sciences [q-bio]/Genetics/Plants genetics, fluids and secretions, Tobacco, Genetics, [SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology, Wheat grain, Triticum, food and beverages, Plant, Plants, Plants, Genetically Modified, bacterial infections and mycoses, Developing endosperm, Mannan, Endosperm, carbohydrates (lipids), Genes, Heterologous expression, Edible Grain, Cell walls
Abstract

International audience; Mannan is a class of cell wall polysaccharides widespread in the plant kingdom. Mannan structure and properties vary according to species and organ. The cell walls of cereal grains have been extensively studied due to their role in cereal processing and to their beneficial effect on human health as dietary fiber. Recently, we showed that mannan in wheat (Triticum aestivum) grain endosperm has a linear structure of beta-1,4-linked mannose residues. The aim of this work was to study the biosynthesis and function of wheat grain mannan. We showed that mannan is deposited in the endosperm early during grain development, and we identified candidate mannan biosynthetic genes expressed in the endosperm. The functional study in wheat was unsuccessful therefore our best candidate genes were expressed in heterologous systems. The endosperm-specificTaCslA12 gene expressed in Pichia pastoris and in an Arabidopsis thaliana mutant depleted in glucomannan led to the production of wheat-like linear mannan lacking glucose residues and with moderate acetylation. Therefore, this gene encodes a mannan synthase and is likely responsible for the synthesis of wheat endosperm mannan.

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