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Among insects, bees are important pollinators, providing many vital ecosystem services. The recent pollinator decline is threatening both their diversity and abundance. One of the main drivers of this decline is the extensive use of pesticides. Neonicotinoids, one of the most popular groups of pesticides, can be toxic to bees. In fact, numerous studies have found that neonicotinoids can cause sublethal effects, which can impair the biology, physiology, and colony survival of the bees. Yet, there are still knowledge gaps, and more research is needed to better understand the interaction between neonicotinoids and bees, especially in the field. A new optical sensor, which can automatically identify flying insects using machine learning, has been created to continuously monitor insect activity in the field. This study investigated the potential use of this sensor as a tool for monitoring the sublethal effects of pesticides on bumblebees. Bombus terrestris workers were orally exposed to field-realistic doses of imidacloprid. Two types of exposures were tested: acute and chronic. The flight activity of pesticide-exposed and non-exposed bumblebees was recorded, and the events of the insect flights recorded by the sensor were used in two ways: to extract the values of the wingbeat frequency and to train machine learning models. The results showed that the trained model was able to recognize differences between the events created by pesticide-exposed bumblebees and the control bumblebees. This study demonstrates the possibility of the optical sensor for use as a tool to monitor bees that have been exposed to sublethal doses of pesticides. The optical sensor can provide data that could be helpful in managing and, ideally, mitigating the decline of pollinators from one of their most major threats, pesticides.

Cimbicidae is a small family of Tenthredinoidea, containing the largest extant true sawflies. Body length variation across the family is mapped on an existing phylogeny, and some potential drivers of body size increase are explored: intrasexual competition between males, and mimicry patterns. Body length varies substantially within Cimbicidae, the largest species being found within the subfamily Cimbicinae. The smallest species occur in Corynidinae; this subfamily has experienced an overall decrease in body size. Initially, body size increase in Cimbicinae is correlated with males becoming larger than females, increase in relative mandible size in both sexes but especially in males, and increased hind femur width/length ratio in males only. Relative mandible size decreases in Cimbex spp. + Odontocimbex and hind femur width/length in male Leptocimbex spp., respectively. The record sizes obtained in Cimbex spp. + Odontocimbex are the result of a further increase in body size, coupled with increase in hind femur width/length ratio in males. Black and yellow body colouration and other potential mimicry patterns are observed in a number of genera of Cimbicinae, but are probably not ground plan features of the subfamily. Outside Cimbicinae, black and yellow body colouration is most frequent in Corynidinae, but it cannot be decided if it is a ground plan trait of this subfamily. Intrasexual competition seems to be more likely to have influenced size increase in Cimbicinae than adaptations to mimicry.