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Chau Shuk Ling., Thesis (M.Phil.)--Chinese University of Hong Kong, 2006., Includes bibliographical references (leaves 103-115)., s in English and Chinese., DECLARATION --- p.i, ACKNOWLEDGEMENT --- p.ii, ABBREVIATIONS --- p.iii, IN ENGLISH --- p.v, IN CHINESE --- p.viii, TABLE OF CONTENTS --- p.xi, Chapter CHAPTER I - --- INTRODUCTION, Chapter 1.1 --- Fluid Transport in Human Colon --- p.1, Chapter 1.2 --- C1' Secretion across the Colonic Epithelium --- p.2, Chapter 1.3 --- Properties of T84 Cells --- p.7, Chapter 1.4 --- General Introduction on Extracellular Calcium Sensing Receptor (CaSR) --- p.8, Chapter 1.5 --- Molecular Structure of CaSR --- p.9, Chapter 1.6 --- CaSR-mediated Intracellular Signaling --- p.13, Chapter 1.7 --- Agonists of CaSR --- p.16, Chapter 1.8 --- Functions of CaSR --- p.18, Chapter 1.8.1 --- Homeostatic Functions of CaSR --- p.18, Chapter 1.8.2 --- Non-Homeostatic Functions of CaSR --- p.18, Chapter 1.9 --- Molecular and Functional Study of CaSR Expressed in Colon --- p.19, Chapter 1.10 --- Objectives of the Present Study --- p.21, Chapter CHAPTER II - --- MATERIALS AND METHODS, Chapter 2.1 --- Solutions and Drugs --- p.22, Chapter 2.2 --- Cell Culture --- p.23, Chapter 2.3 --- Western Blot --- p.26, Chapter 2.4 --- Simultaneous Measurement of Short-Circuit Current (Isc) and Intracellular Calcium Concentration ([Ca2+]i) --- p.27, Chapter 2.4.1 --- Measurement of Isc and Transepithelial Resistance with Ussing Chamber --- p.27, Chapter 2.4.2 --- Simultaneous Measurement of Isc and [Ca2+]i --- p.29, Chapter 2.5 --- Measurement of Isc with Conventional Ussing Chamber --- p.32, Chapter 2.6 --- Measurement of Intracellular cAMP Accumulation with Enzyme-Linked Immunosorbent Assay --- p.34, Chapter 2.7 --- Data Analysis --- p.34, Chapter CHAPTER III - --- RESULTS, Chapter 3.1 --- Expression of CaSR in T84 Cell Monolayers --- p.36, Chapter 3.2 --- Poly L-arginine Induced an Increase in Isc --- p.38, Chapter 3.2.1 --- Simultaneous Measurement of Isc and [Ca2+ ]i in Response to Poly L-arginine --- p.38, Chapter 3.2.2 --- Interaction Between Poly L-arginine and Forskolin --- p.50, Chapter 3.2.3 --- Ionic Mechanism of Isc Stimulated by Poly L-arginine --- p.58, Chapter 3.2.3.1 --- Involvement of C1- in Isc Stimulated by Poly L-arginine --- p.58, Chapter 3.2.3.2 --- Involvement of Basolateral K+ Channels in Isc Stimulated by Poly L-arginine --- p.61, Chapter 3.2.4 --- Signaling Pathways Underlying the Isc Response to Poly L-arginine --- p.73, Chapter CHAPTER IV - --- DISSCUSION, Chapter 4.1 --- Presence of CaSR in T84 Cell Monolayers --- p.78, Chapter 4.2 --- Simultaneous Measurement of Isc and [Ca2+ ]i upon Application of Poly L-arginine --- p.82, Chapter 4.3 --- Ionic Mechanism Underlying the Increase in Isc Stimulated by Poly L-arginine --- p.87, Chapter 4.4 --- Signaling Pathway Underlying the Action of Poly L-arginine --- p.93, Chapter 4.5 --- Does CaSR Mediate Poly L-arginine-evoked C1- Secretion across T84 Cell Monolayers? --- p.96, Chapter 4.6 --- Future Study --- p.101, REFERENCES --- p.103, http://library.cuhk.edu.hk/record=b5893054, Use of this resource is governed by the terms and conditions of the Creative Commons “Attribution-NonCommercial-NoDerivatives 4.0 International” License (http://creativecommons.org/licenses/by-nc-nd/4.0/)