1. Fine-tuning of key parameters to enhance biomass and nutritional polyunsaturated fatty acids production from Thraustochytrium sp.
- Author
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Chauhan, Ajeet Singh, Patel, Anil Kumar, Singhania, Reeta Rani, Vadrale, Akash Pralhad, Chen, Chiu-Wen, Giri, Balendu Sheker, Chang, Jo-Shu, and Dong, Cheng-Di
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EICOSAPENTAENOIC acid , *DOCOSAHEXAENOIC acid , *BIOMASS , *UNSATURATED fatty acids , *OMEGA-3 fatty acids , *PROCESS optimization - Abstract
[Display omitted] • Optimization led to 32.30 % increase in Thraustochytrium biomass (9.88 g/L). • Lipid yield experienced a significant surge, rising by 31.92 % to reach 6.57 g/L. • DHA concentration exhibited an impressive increase of 69.91 %, reaching 1.63 g/L. • EPA and DPA exhibited notable enhancements by 82.69 and 31.47%, respectively. • MANOVA showed significant optimization effect on biomass, and DHA yield (p < 0.05). The escalating demand for long-chain polyunsaturated fatty acids (PUFAs) due to their vital health effects has deepened the exploration of sustainable sources. Thraustochytrium sp. stands out as a promising platform for omega-3 and 6 PUFA production. This research strategically optimizes key parameters: temperature, salinity, pH, and G:Y:P ratio and the optimized conditions for maximum biomass, total lipid, and DHA enhancement were 28 °C, 50 %, 6, and 10:1:2 respectively. Process optimization enhanced 32.30 and 31.92 % biomass (9.88 g/L) and lipid (6.57 g/L) yield. Notably, DHA concentration experienced a substantial rise of 69.91 % (1.63 g/L), accompanied by notable increases in EPA and DPA by 82.69 % and 31.47 %, respectively. MANOVA analysis underscored the statistical significance of the optimization process (p < 0.01), with all environmental factors significantly influencing biomass and lipid data (p < 0.05), particularly impacting DHA production. Thraustochytrium sp. can be a potential source of commercial DHA production with the fine-tuning of these key process parameters. [ABSTRACT FROM AUTHOR]
- Published
- 2024
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