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Your search keyword '"Che, Leiping"' showing total 22 results
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22 results on '"Che, Leiping"'

10. 基于数字全息显微层析的癌细胞空泡化成像研究

Author

刘雅坤 Liu Yakun, 肖文 Xiao Wen, 车蕾平 Che Leiping, 李胤嘉 Li Yinjia, 肖溪 Xiao Xi, 王皓 Wang Hao, and 潘锋 Pan Feng

Subjects
Electrical and Electronic Engineering, Atomic and Molecular Physics, and Optics, Electronic, Optical and Magnetic Materials
Published
2022

16. A Quantitative Research on Human Mesenchymal Stem Cell Morphological Changes by Trypsin-EDTA via Digital Holographic Microscopy

Author

Pan Feng, Che Leiping, Cao Runyu, Xiaosu Yi, Zeng Qiang, Tang Zhihui, Xiao Wen, and Chen Litong

Subjects
Materials science, Spatial filter, Cell, Mesenchymal stem cell, Phase-contrast imaging, Trypsin, law.invention, medicine.anatomical_structure, law, Height increased, medicine, Digital holographic microscopy, sense organs, skin and connective tissue diseases, Beam splitter, Biomedical engineering, medicine.drug
Abstract

Morphological changes of a single human mesenchymal stem cell by trypsin-EDTA were measured via digital holographic microscopy. Cell contact area decreased, the maximum height increased and volume fluctuated as cell changed from flat to stereoscopic.

Published
2019

17. Automatic removal of phase aberration in holographic microscopy for drug sensitivity detection of ovarian cancer cells

Author

Feng Pan, Pietro Ferraro, Che Leiping, Li Xiaoping, Wen Xiao, and JinJin Liu

Subjects
Microscope, genetic structures, business.industry, Computer science, Holography, Image processing, biomedical, Atomic and Molecular Physics, and Optics, Electronic, Optical and Magnetic Materials, law.invention, Interferometry, Biological specimen, law, Microscopy, microscopy, cells, holography, Computer vision, Sensitivity (control systems), Artificial intelligence, Electrical and Electronic Engineering, business, Digital holography
Abstract

Real-time and long-term monitoring of the morphological changes of cells in biomedical science is highly desired. Quantitative phase imaging (QPI) obtained by various interferometric methods is the ideal tool for monitoring such processes as it allows to get quantitative information and thus assessing the right response on cell behaviors. Among QPI, digital holography (DH) in microscope configuration is a powerful tool as it is tolerant versus defocusing and for this reason is able to compensate for eventual defocusing effect during long time-lapse recording. Moreover, DH dynamic phase imaging for biological specimens has several advantages, namely non-invasive, label-free, and high-resolution. However, in DH, one of the main limitations is due to the need compensate aberrations due to the optical components in the object beam. In fact, the image of the object is inevitably embedded in aberrations due to the microscope objective (MO) and other optical components in the optical setup. Here, we propose an automatic and robust phase aberration compensation method based on a synthetic difference (SD) image process. The method is able to detect automatically object-free regions. From such regions, hologram's aberrations can be accurately evaluated and cleaned up in the final QPI maps. Thanks to our method, temporal evolutions of cell morphological parameters were quantitatively analyzed, hence helping in studying the drug sensitivity of ovarian cancer cells. The experimental results demonstrated that the proposed method could robustly separate the object-free region from the distorted phase image and automatically compensate the total aberrations without any manual interventions, extra components, prior knowledge of the object, and optical setup. (C) 2020 Optical Society of America under the terms of the OSA Open Access Publishing Agreement

Published
2020

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