Search

Your search keyword '"Chellappa, Duraisamy"' showing total 31 results
Start Over Author "Chellappa, Duraisamy"
31 results on '"Chellappa, Duraisamy"'

11. Facile Synthesis of Highly Sensitive, Red-Emitting, Fluorogenic Dye for Microviscosity and Mitochondrial Imaging in Embryonic Stem Cells

Author

Sufi O Raja, Chellappa Duraisamy, Gandhi Sivaraman, Ananya Mukherjee, and Akash Gulyani

Subjects
Chemistry, 02 engineering and technology, General Chemistry, Mitochondrion, 010402 general chemistry, 021001 nanoscience & nanotechnology, 01 natural sciences, Embryonic stem cell, Fluorescence, 0104 chemical sciences, Highly sensitive, Microviscosity, Fluorescence intensity, Biochemistry, Live cell imaging, Biophysics, Cellular dynamics, 0210 nano-technology
Abstract

Bright, sensitive fluorescent probes that respond to changes in the cellular microenvironment are extremely valuable for imaging cellular dynamics. We report a simple, one-step synthesis of a new hemicaynine (HC-1) dye as a sensitive, red-emitting (λmax-610 nm) fluorogenic probe for micro-viscosity and local order in diverse environments, including live cells. HC-1 responds to increasing micro-viscosity through changes in fluorescence intensity and lifetime, and is sensitive enough to report dynamic micellar self-assembly. While HC-1 shows properties of a molecular ‘rotor’, time-dependent density functional theoretical analysis reveals that in HC-1, an inhibition of photo-isomerization in viscous environment is the likely cause of fluorescence enhancement. HC-1 localizes to mitochondria in live cells and responds to mitochondrial ordering through a significant increase in fluorescence. Strikingly, we show that HC-1 is also a sensitive probe for the spatial heterogeneity of mitochondrial organization in embryonic stem cells as well as dynamic remodeling of the mitochondria in early-differentiated cells.

Published
2017

29. Hg2+ mediated quinazoline ensemble for highly selective recognition of Cysteine.

Author

Anand, Thangaraj, Sivaraman, Gandhi, and Chellappa, Duraisamy

Subjects
*MERCURY, *QUINAZOLINE, *CYSTEINE, *MOLECULAR recognition, *CHEMICAL synthesis, *DENSITY functional theory
Abstract

Highlights: [•] A quinazoline A1 derived was synthesized and used to recognize Hg2+. [•] The Hg2+ detection limit (3.5×10−7 molL−1) is reported. [•] This fluorescence change was further supported by DFT/TD-DFT calculations. [•] The probe A1 +Hg2+ ensemble is also further successfully utilized for detection of the Cysteine. [•] This system can also be applied in real samples. [ABSTRACT FROM AUTHOR]

Published
2014
Full Text
View/download PDF

30. Charge transfer based “turn-on” chemosensor for Zn2 + ion recognition using new triaryl pyrazoline derivative.

Author

Jeyanthi, Dharmaraj, Iniya, Murugan, Krishnaveni, Karuppiah, and Chellappa, Duraisamy

Subjects
*CHARGE transfer, *CHEMORECEPTORS, *ZINC ions, *NUCLEAR magnetic resonance, *STOICHIOMETRY
Abstract

The fluoroionophore PY serves as a selective and fluorimetric chemosensor for Zn 2 + based on charge transfer (CT). A mechanism for the binding mode was proposed based on fluorescence changes, NMR experiments and theoretical calculations. The 1:1 stoichiometry between Zn 2 + and the sensor was deduced from Job's plot. The addition of EDTA quenches the fluorescence of PY.Zn 2 + complex offers PY as a reversible chemosensor. [ABSTRACT FROM AUTHOR]

Published
2016
Full Text
View/download PDF

31. Triazole based ratiometric fluorescent probe for Zn2+ and its application in bioimaging.

Author

Iniya, Murugan, Jeyanthi, Dharmaraj, Krishnaveni, Karuppiah, Mahesh, Ayyavu, and Chellappa, Duraisamy

Subjects
*TRIAZOLES, *FLUORESCENT probes, *ZINC, *BIO-imaging sensors, *WAVELENGTHS, *MOLECULAR probes
Abstract

Highlights: [•] Triazole based ratiometric fluorescent sensor for Zn2+ was designed based on ESIPT mechanism. [•] The probe displays long excitation wavelength and potential to quantify the concentration of Zn2+. [•] High stability and cell-permeability is an additional feature of probe. [•] The probe is applied successfully for recognizing intracellular Zn2+ within living cells. [ABSTRACT FROM AUTHOR]

Published
2014
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results