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Phyllanthus emblica L. (P. emblica L.) is a widely consumed healthy fruit known for its taste and benefits. This study used association rule mining to demonstrate its anticancer properties, analyzing its 65 chemical components, 1767 related targets, and 4282 efficacy items. Myricetin was identified as a key component targeting CA2, which is involved in cancer pathways. The research suggested P. emblica L.'s potential against non-small cell lung cancer and esophageal cancer, which was supported by experiments showing significant growth inhibition in related cancer cells. This approach offers a more targeted and convincing understanding of P. emblica L.'s effects, improving upon traditional food research methods. [ABSTRACT FROM AUTHOR]

Vertical-cavity surface-emitting lasers (VCSELs) are essential for exhibiting single-transverse-mode output characteristics, which are critical for applications in quantum sensing, optical interconnection, and laser printing. In this study, we achieved stable single-transverse-mode lasing using extended-2λ-cavity with an oxide aperture diameter of 7.08 μm. The device demonstrated a high output power of 6.8 mW and a narrow linewidth of 49.8 MHz at room temperature. Additionally, it maintained stable single-mode emission at 794.8 nm and achieved a side-mode suppression ratio (SMSR) exceeding 40 dB within the temperature range of 25 °C~85 °C, thereby meeting the requirements of 87Rb atom quantum sensors. The fabricated device obtained high-power and narrow linewidth single-transverse-mode operation by a monolithic extended cavity without introducing additional processing procedures, which is expected to promote the commercial viability of VCSELs in quantum sensing. [ABSTRACT FROM AUTHOR]

Sewage sludge odorous gas release is a key barrier to resource utilization, and conditioners can mitigate the release of sulfur-containing gases. The gas release characteristics and sulfur compound distribution in pyrolysis products under both single and composite conditioning strategies of CaO, Fe2O3, and FeCl3 were investigated. This study focused on the inhibition mechanisms of these conditioners on sulfur-containing gas emissions and compared the theoretical and experimental sulfur content in the products to evaluate the potential synergistic effects of the composite conditioners. The findings indicated that at 650 °C, CaO, Fe2O3, and FeCl3 inhibited H2S release by 35.8%, 23.2%, and 9.1%, respectively. Notably, the composite of CaO with FeCl3 at temperatures ranging from 350 to 450 °C and the combination of Fe2O3 with FeCl3 at 650 °C were found to exert synergistic suppression on H2S emissions. The strongly alkaline CaO inhibited the metathesis reaction between HCl, a decomposition product of FeCl3, and the sulfur-containing compounds within the sewage sludge, thereby exerting a synergistic suppression on the emission of H2S. Conversely, at temperatures exceeding 550 °C, the formation of Ca-Fe compounds, such as FeCa2O4, appeared to diminish the sulfur-fixing capacity of the conditioners, resulting in increased H2S emissions. For instance, the combination of CaO and FeCl3 at 450 °C was found to synergistically reduce H2S emissions by 56.3%, while the combination of CaO and Fe2O3 at 650 °C synergistically enhances the release of H2S by 23.6%. The insights gained from this study are instrumental in optimizing the pyrolysis of sewage sludge, aiming to minimize its environmental footprint and enhance the efficiency of resource recovery. [ABSTRACT FROM AUTHOR]

• Development of a Rapid and Efficient Detection System: We have developed an efficient RPA- Pf Ago detection system for the MTHFR C677T polymorphism, streamlining the genotyping process. • RPA Reaction, gDNA, and RPA- Pf Ago Optimization: Through meticulous optimization, we enhanced the efficiency and sensitivity of the MTHFR C677T detection system. • Clinical Validation: Our method has been clinically validated on samples and showed 100% concordance with Sanger sequencing, confirming the precision of the method. • Potential for Rapid, On-Site Genotyping: The RPA- Pf Ago system we have developed offers a tool for rapid on-site genotyping. This study introduces an efficient RPA- Pf Ago detection system for the MTHFR C677T polymorphism, proposing a potential strategy to simplify the genotyping process. By optimizing recombinase polymerase amplification (RPA) with Pyrococcus furiosus Argonaute (Pf Ago) nucleases, we achieved DNA amplification at a constant temperature. The assay was fine-tuned through meticulous primer and guide DNA selection, with optimal conditions established at 2.0 µL of MgAc, a reaction temperature of 42 °C, and a 10-minute reaction time for RPA. Further optimization of the Pf Ago cleavage assay revealed the ideal concentrations of MnCl 2 , guide DNA, molecular beacon probes, the Pf Ago enzyme, and the RPA product to maximize sensitivity and specificity. Clinical validation of 20 samples showed 100% concordance with Sanger sequencing, confirming the method's precision. The RPA- Pf Ago system is a promising tool for on-site genotyping, with broad applications in personalized medicine and disease prevention. [ABSTRACT FROM AUTHOR]