29 results on '"Chen, Qi-Min"'
Search Results
2. Sensitivity analysis of regional rainfall-induced landslide based on UAV photogrammetry and LSTM neural network
- Author
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Zhao, Lian-heng, Xu, Xin, Lyu, Guo-shun, Huang, Dong-liang, Liu, Min, and Chen, Qi-min
- Published
- 2023
- Full Text
- View/download PDF
3. A new indicator cell line established to monitor bovine foamy virus infection
- Author
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Guo, Hong-yan, Liang, Zhi-bin, Li, Yue, Tan, Juan, Chen, Qi-min, and Qiao, Wen-tao
- Published
- 2011
- Full Text
- View/download PDF
4. Subcellular localization analysis of bovine foamy virus Borf1 protein
- Author
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Tan, Juan, Wu, Kai, Chang, Rui, Chen, Qi-min, Geng, Yun-qi, and Qiao, Wen-tao
- Published
- 2008
- Full Text
- View/download PDF
5. Tight filum terminale syndrome in children: analysis based on positioning of the conus and absence or presence of lumbosacral lipoma
- Author
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Bao, Nan, Chen, Zhi-Hua, Gu, Shuo, Chen, Qi-Min, Jin, Hui-Ming, and Shi, Cheng-Ren
- Published
- 2007
- Full Text
- View/download PDF
6. Construction and characterization of a new simian/human immunodeficiency viruses clone carrying an env gene derived from a CRF07_BC strain
- Author
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LI, Yue, YANG, Gui-bo, CHEN, Qi-min, LIU, Qiang, MENG, Zhe-feng, GENG, Yun-qi, QIAO, Wen-tao, and SHAO, Yi-ming
- Published
- 2009
- Full Text
- View/download PDF
7. Detection and analysis of bovine foamy virus infection by an indicator cell line
- Author
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MA, Zhe, AO, Wen-tao QI, XUAN, Cheng-hao, XIE, Jin-hui, CHEN, Qi-min, and GENG, Yun-qi
- Published
- 2007
8. Molecular cloning and in vitro evaluation of an infectious simian-human immunodeficiency virus containing env of a primary Chinese HIV-1 subtype C isolate
- Author
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Wu, Ying Yun, Hong, Kun Xue, Chenine, Agnès-Laurence, Whitney, James B., Chen, Qi Min, Geng, Yun Qi, Ruprecht, Ruth M., and Shao, Yi Ming
- Published
- 2005
9. New frontiers in the treatment of colorectal cancer: Autophagy and the unfolded protein response as promising targets
- Author
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Mokarram, Pooneh, primary, Albokashy, Mohammed, additional, Zarghooni, Maryam, additional, Moosavi, Mohammad Amin, additional, Sepehri, Zahra, additional, Chen, Qi Min, additional, Hudecki, Andrzej, additional, Sargazi, Aliyeh, additional, Alizadeh, Javad, additional, Moghadam, Adel Rezaei, additional, Hashemi, Mohammad, additional, Movassagh, Hesam, additional, Klonisch, Thomas, additional, Owji, Ali Akbar, additional, Łos, Marek J., additional, and Ghavami, Saeid, additional
- Published
- 2017
- Full Text
- View/download PDF
10. Bovine HEXIM1 inhibits bovine immunodeficiency virus replication through regulating BTat-mediated transactivation
- Author
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Guo, Hong-yan, primary, Ma, Yong-gang, additional, Gai, Yuan-ming, additional, Liang, Zhi-bin, additional, Ma, Jing, additional, Su, Yang, additional, Zhang, Qi-cheng, additional, Chen, Qi-min, additional, and Tan, Juan, additional
- Published
- 2013
- Full Text
- View/download PDF
11. Mevalonate Cascade and Small Rho GTPase in Spinal Cord Injury
- Author
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Chen, Qi Min, Iqbal, Mohamed Ariff, Nagakannan, Pandian, and Eftekharpour, Eftekhar
- Abstract
The mevalonate pathway has been extensively studied for its involvement in cholesterol synthesis. Inhibition of this pathway using statins (3-Hydroxy-3-methylglutaryl-coenzyme A reductase inhibitors; HMGR inhibitors) is the primarily selected method due to its cholesterol-lowering effect, making statins the most commonly used (86-94%) cholesterol-lowering drugs in adults. This pathway has several other by-products that are affected by statins including GTPase molecules (guanine triphosphate-binding kinases), such as Rho/Rho-associated coiled kinase (ROCK) kinases, that are implicated in other diseases, including those of the central nervous system (CNS). These molecules control several aspects of neural cell life including axonal growth, cellular migration, and cell death, and therefore, are of increasing interest in the field of spinal cord injury (SCI). Limited regeneration capacity of nerve fibers in adult CNS has been considered the main obstacle for finding a SCI cure. Over the past two decades, the identity of inhibitory factors for regeneration has been widely investigated. It is well-established that the Rho/ROCK kinase system is specifically activated by the components of damaged spinal cord tissue, including oligodendrocytes and myelin, as well as extracellular matrix. This has led many groups to hypothesize that statin therapy may in fact enhance the current neurorestorative approaches. In this mini-review, a summary of SCI pathophysiology is discussed and the current literature targeting the regeneration obstacles in SCI are reviewed, with special attention to recent publications of the past decade. In addition, we focus on the current literature involving the use of pharmacological and molecular inhibitors of small GTPase molecules for treatment of neurotrauma. Inhibiting these molecules has been shown to increase neuroprotection, enhance axonal regeneration, and facilitate the implementation of cell replacement therapies. Based upon available literature, the need for clinical trials involving targeted inhibition of GTPase molecules remains strong. Some of these drugs are widely used for other diseases, and therefore re-purposing their application for neurotrauma can be fasttracked. These approaches can potentially modify the inhibitory environment of nervous tissue to allow the spontaneous repair capacity of injured tissue.
- Published
- 2017
12. Mevalonate Cascade and Neurodevelopmental and Neurodegenerative Diseases: Future Targets for Therapeutic Application
- Author
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Marzban, Hassan, Kong, Jiming, Mehr, Shahram Ejtemaei, Vriend, Jerry, Li, Jiasi, Guan, Teng, Chung, Seunghyuk, Mirzaei, Nima, Marzban, Adel, Shojaei, Shahla, Badbezanchi, Ilnaz, Chen, Qi Min, Rahimi-Balaei, Maryam, Ashtari, Niloufar, and Jiao, Xiaodan
- Abstract
The mevalonate cascade is a key metabolic pathway that regulates a variety of cellular functions and is thereby implicated in the pathophysiology of most brain diseases, including neurodevelopmental and neurodegenerative disorders. Emerging lines of evidence suggest that statins and Rho GTPase inhibitors are efficacious and have advantageous properties in treatment of different pathologic conditions that are relevant to the central nervous system. Beyond the original role of statins in lowering cholesterol synthesis, they have anti-inflammatory, antioxidant and modulatory effects on signaling pathways. Additionally, Rho GTPase inhibitors and statins share the mevalonate pathway as a common target of their therapeutic actions. In this review, we discuss potential mechanisms through which these drugs, via their role in the mevalonate pathway, exert their neuroprotective effects in neurodegenerative and neurodevelopmental disorders.
- Published
- 2017
13. RNA Silencing Suppressor p19 Regulates The Expressions of Cell Cycle Related Genes*
- Author
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LIU, Li, primary, LI, Jian, additional, XU, Yu-Ping, additional, QIAO, Wen-Tao, additional, CHEN, Qi-Min, additional, and GENG, Yun-Qi, additional
- Published
- 2009
- Full Text
- View/download PDF
14. Cloning and characterization of a novel intracellular protein p48.2 that negatively regulates cell cycle progression
- Author
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Yang, Fan, primary, Xu, Yu-Ping, additional, Li, Jian, additional, Duan, Su-Su, additional, Fu, Ying-Jie, additional, Zhang, Yun, additional, Zhao, Yan, additional, Qiao, Wen-Tao, additional, Chen, Qi-Min, additional, Geng, Yun-Qi, additional, Che, Chang-Yan, additional, Cao, Ying-Li, additional, Wang, Ying, additional, Zhang, Lei, additional, Long, Lin, additional, He, Jie, additional, Cui, Quan-Cai, additional, Chen, Shu-Chang, additional, Wang, Shu-Hui, additional, and Liu, Li, additional
- Published
- 2009
- Full Text
- View/download PDF
15. Detection of Bovine Immunodeficiency Virus Antibodies in Cattle by Western Blot Assay with Recombinant Gag Protein
- Author
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Zhang, Shucheng, primary, Xue, Wenzhi, additional, Wood, Charles, additional, Chen, Qi-Min, additional, Kapil, Sanjay, additional, and Minocha, Harish C., additional
- Published
- 1997
- Full Text
- View/download PDF
16. Sequence of the gene coding for ribosomal protein S8 of Xenopus laevis
- Author
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Mariottini, Paolo, primary, Bagni, Claudia, additional, Francesconi, Anna, additional, Cecconi, Francesco, additional, Serra, Martin J., additional, Chen, Qi-Min, additional, Loreni, Fabrizio, additional, Annesi, Flavia, additional, and Amaldi, Francesco, additional
- Published
- 1993
- Full Text
- View/download PDF
17. THE DEVELOPMENT OF ØLFD-5 DARRIEUS WIND TURBINE
- Author
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Ye Ji-sheng, Chen Qi-min, and Yang Mao-feng
- Subjects
Engineering ,business.industry ,Rotor (electric) ,Aerodynamics ,Structural engineering ,Turbine ,Wind speed ,law.invention ,law ,Torque ,business ,Wells turbine ,Darrieus wind turbine ,Wind tunnel ,Marine engineering - Abstract
This paper describes the development of vertical axis Darrieus Wind turbine at the Department of Thermal Engineering, Qinghua University. On the basis of the aerodynamic analysis, structure dynamics analysis and wind tunnel model testing, a 5. Darrieus wind turbine had been designed, constructed and installed at the field site for performance testing. Special integral mould was developed for serial production of glass-fibre reinforced plastic blades. Sovonius rotors are used to make the wind turbine self-starting. Overspeed is controlled by aerodynamic spoilers. Optimum operating conditions of wind turbine for different wind speeds can be controlled by automatic load circuit. Variable-speed-constant-frequency A.C. and constant-voltage D.C. power generations have been tested with this wind turbine. Instruments for measuring the wind speed, rotor speed, rotor torque, electric power output, rotor shaft vibration and blade stresses are equiped to register the performance data. Field testing results are also presented in this paper.
- Published
- 1982
18. INVITED AND CONTRIBUTED AUTHORS
- Author
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B. Adamson, H.M. Agnew, Bai Lin, R.L. Bannister, Bao Han-chen, P.S. Basile, E.J. Beiting, S.B. Bennett, M. Blase, D.J. Boyle, J. Braymer, E.S. Burcher, A.J. Butler, M. Calvin, W.W.S. Charters, Chen Ming Shao, Chen Qi-min, R.J. Cheng, P. Clark, R.N. Clark, M. Collares-Pereira, W. Cooper, R.B. Covell, B.P. Cucolo, B. Dambly, J.A. Damm, E.E. David, K.M. Davis, M. Dicks, H.M. Draper, F.S. Dubin, A. Dybbs, J.S. Edmonds, A.J. Eggers, P.J. Emmerman, J.C.C. Fan, D.L. Ford, C.E. Francis, E. Gloyna, J.M. Gordon, R. Goulard, J. Gretz, Guan Yu-de, Guo Bao-sen, Guo Gan-ci, D.O. Hall, J. Harte, A. Hildebrandt, K.C. Hoffman, Jin Fa-nan, V. Jonguitud, V.W. Kaczynski, T.M. Keevin, D.L. Kirkpatrick, R.A. Kumar, J.F. LaBounty, J.A. Larrimore, T.K. Lau, Le Wei-liang, P. Leung, Li You-jia, Lin Hao, Lin Ze-zu, L. Liu, Liu Zhao-xu, Lu Qin-kan, R. Lyman, C.D. MacCracken, R.W.G. Macdonald, M.E. McCormick, W.R. McCown, A.E. McGarity, R. McKinney, M.R. Mack, M. Masoero, S.L. Mathieu, P.H. Miller, E. Morofsky, D.L. Murphree, J.J. O'Gallagher, J. O'Hara, I. Oliker, Ou Guang-yao, P. Palmedo, S.C. Peek, N.A. Phillips, M. Pinnoti, J.L. Plummer, A. Rabl, L.P. Raymond, M. Rieber, C.E. Roedder, A.H. Rosenfeld, D.P. Russell, R.A. Salib, R.J. Santoro, S. Sargent, M. Savitz, H.W. Scheid, E.B. Schultz, S. Schweitzer, R.C. Seamans, H.G. Semerjian, Shi Jia-yang, T.T. Shih, P.W. Shuldiner, G.J. Silvestri, K. Snail, R.H. Socolow, S.L. Soo, B. Sorensen, R.E. Stickel, H. Tabor, Tan Hong-guang, T.B. Taylor, Tong Wei, Wang Bu-xuan, Wang Ding-zhu, Wang Ping-yang, Wang Huan-chen, Wang Ying-luo, R. Winston, L. Woodcock, M.P. Wnuk, Wu Jing, Yang Mao-feng, S.C. Yao, Ye Ji-sheng, K.E. Yeager, Ying Shu-fang, Y.Y. Yu, Y. Zarmi, Zhang Ming-tao, Zhang Zhen-ming, Zhang Zhi, Zhao Dian-wu, Zhao Lian-sheng, Zhao Yu-kun, Zhao Zeng-guong, Zheng Ding-rong, Zheng Nai-bai, Zhou Pei-yuan, Zhu Ya-jie, and Zhu Xiao-zhang
- Published
- 1982
19. CONFERENCE COMMITTEES
- Author
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S.W. Yuan, Lin Hang-xiong, A. Robert Abboud, Melvin Calvin, Alfred J. Eggers, King H. Fung, Congressman Dan Glickman, Earnest Gloyna, Walter J. Hickel, Joe Kilgore, David Lewis, Harold Liebowitz, Frank Marble, Congressman Nicholas Mavroules, S.S. Penner, Courtland D. Perkins, Robert Seamans, Leonard Woodcock, Paul Dembling, George Ho, Joseph Lane, William Corcoran, George Ellowitz, Hugh Miller, Ervin Rodin, Edward Saibel, John Whitesides, Robert N. Miranda, Joanne Redding, Raymond Bisplinghoff, Louis Divone, John Dougherty, Lawrence Galowin, Anthony J. Gorski, Jack Hollander, Frank Kreith, James L. Plummer, Ward Sanders, Larry Tombough, Leslie Levine, Vujica Yevjevich, Ma Hong, Wang Gan-chang, Li Ruai, Wu Zhong-hua, He Kang, Wu Heng, Zhou Pei-yuan, Zhang Wei, Yang Ji-ke, Zhao Zeng-yu, Hou Xiang-lin, Gao Yang-wen, Qian Xue-sen, Huan Xin-min, Pei Li-sheng, Xue Bao-ding, Zhu Ya-jie, Lu Ying-zhong, Qin Tong-luo, Wang Ping-yang, Lan Tian-fang, Lio Baao, Zhu Xia-zhang, Wu Jing, Wu Xiang-gan, Shan Yon-ting, Wang De-xi, Mao Yu-shi, Chen Ru-sen, Chen Ming-shao, Chen Qi-min, Huan Zhi-jie, Huang Hong-ning, Lu Qin-kan, Yao Fu-min, Gung Bao, and Chai Yi-han
- Published
- 1982
20. [Characteristics of main layer and regeneration layer of Haloxylon ammodendron plantations at different ages on the southern edge of the Gurbantunggut Desert, Northwest China].
- Author
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Chen QM, Luo QH, Ning HS, Zhao CY, and Duan WB
- Subjects
- China, Trees, Chenopodiaceae, Soil
- Abstract
The population structure characteristics, natural regeneration, and the influential factors of Haloxylon ammodendron plantations at six different stand ages on the southern edge of the Gurbantunggut Desert were studied. The results showed that H. ammodendron plantation at the stand age of 7 could naturally regenerate. At the stand age of 17, the densities of the seedlings (<30 cm height), saplings (30≤H<50 height), and small trees (≥50 cm height) reached optimal class, and the mean height and base diameter of the small tress reached 1.10 m and 1.91 cm, respectively. The parent trees in H. ammodendron plantation at the stand age of 20 grew best. The height of 35% individuals grew up to 2.50-3.00 m, and the basal stem diameter of 23.1% individuals grew up to 8.00-10.00 cm. The height and diameter growth of the parent trees in H. ammodendron plantation at the stand age of 33 apparently declined, but the regeneration ability by natural seed dispersal was still strong. The regeneration density of natural seed dispersal showed the greatest correlation with the available nitrogen content in 0-100 cm soil layer (0.87), followed by the soil rapidly available phosphorus content (0.84) and the soil water content (0.79). The soils with pH 8.1-8.6 did not limit the nutrient growth of the regeneration layer. In the main stand layer, the individual density of whole regeneration layer showed the greatest correlation with the biomass of the parent trees (0.77), while the density of regeneration layer of the small trees showed the greatest correlation with the planting density (0.71) and the age of the parent trees (0.70).
- Published
- 2017
- Full Text
- View/download PDF
21. Mevalonate Cascade and Small Rho GTPase in Spinal Cord Injury.
- Author
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Eftekharpour E, Nagakannan P, Iqbal MA, and Chen QM
- Subjects
- Animals, Axons metabolism, Cholesterol metabolism, Humans, Hydroxymethylglutaryl-CoA Reductase Inhibitors therapeutic use, Metabolic Networks and Pathways drug effects, Myelin Sheath drug effects, Neural Stem Cells transplantation, Neurons metabolism, Signal Transduction, Spinal Cord Injuries therapy, Stem Cell Transplantation, Mevalonic Acid metabolism, Spinal Cord Injuries drug therapy, Spinal Cord Injuries metabolism, rho GTP-Binding Proteins antagonists & inhibitors, rho GTP-Binding Proteins metabolism, rho-Associated Kinases metabolism
- Abstract
The mevalonate pathway has been extensively studied for its involvement in cholesterol synthesis. Inhibition of this pathway using statins (3-Hydroxy-3-methylglutaryl-coenzyme A reductase inhibitors; HMGR inhibitors) is the primarily selected method due to its cholesterol-lowering effect, making statins the most commonly used (86-94%) cholesterol-lowering drugs in adults. This pathway has several other by-products that are affected by statins including GTPase molecules (guanine triphosphate-binding kinases), such as Rho/Rho-associated coiled kinase (ROCK) kinases, that are implicated in other diseases, including those of the central nervous system (CNS). These molecules control several aspects of neural cell life including axonal growth, cellular migration, and cell death, and therefore, are of increasing interest in the field of spinal cord injury (SCI). Limited regeneration capacity of nerve fibers in adult CNS has been considered the main obstacle for finding a SCI cure. Over the past two decades, the identity of inhibitory factors for regeneration has been widely investigated. It is well-established that the Rho/ROCK kinase system is specifically activated by the components of damaged spinal cord tissue, including oligodendrocytes and myelin, as well as extracellular matrix. This has led many groups to hypothesize that statin therapy may in fact enhance the current neurorestorative approaches. In this mini-review, a summary of SCI pathophysiology is discussed and the current literature targeting the regeneration obstacles in SCI are reviewed, with special attention to recent publications of the past decade. In addition, we focus on the current literature involving the use of pharmacological and molecular inhibitors of small GTPase molecules for treatment of neurotrauma. Inhibiting these molecules has been shown to increase neuroprotection, enhance axonal regeneration, and facilitate the implementation of cell replacement therapies. Based upon available literature, the need for clinical trials involving targeted inhibition of GTPase molecules remains strong. Some of these drugs are widely used for other diseases, and therefore re-purposing their application for neurotrauma can be fasttracked. These approaches can potentially modify the inhibitory environment of nervous tissue to allow the spontaneous repair capacity of injured tissue., (Copyright© Bentham Science Publishers; For any queries, please email at epub@benthamscience.org.)
- Published
- 2017
- Full Text
- View/download PDF
22. Mevalonate Cascade and Neurodevelopmental and Neurodegenerative Diseases: Future Targets for Therapeutic Application.
- Author
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Jiao X, Ashtari N, Rahimi-Balaei M, Chen QM, Badbezanchi I, Shojaei S, Marzban A, Mirzaei N, Chung S, Guan T, Li J, Vriend J, Mehr SE, Kong J, and Marzban H
- Subjects
- Animals, Brain embryology, Brain growth & development, Brain metabolism, Cell Line, Cholesterol metabolism, Enzyme Inhibitors pharmacology, Enzyme Inhibitors therapeutic use, Humans, Hydroxymethylglutaryl-CoA Reductase Inhibitors therapeutic use, Metabolic Networks and Pathways drug effects, Mevalonic Acid pharmacology, Neural Tube embryology, Neural Tube growth & development, Neural Tube metabolism, Signal Transduction drug effects, rho GTP-Binding Proteins antagonists & inhibitors, rho GTP-Binding Proteins metabolism, Hydroxymethylglutaryl-CoA Reductase Inhibitors pharmacology, Mevalonic Acid metabolism, Neurodegenerative Diseases drug therapy, Neurodegenerative Diseases metabolism
- Abstract
The mevalonate cascade is a key metabolic pathway that regulates a variety of cellular functions and is thereby implicated in the pathophysiology of most brain diseases, including neurodevelopmental and neurodegenerative disorders. Emerging lines of evidence suggest that statins and Rho GTPase inhibitors are efficacious and have advantageous properties in treatment of different pathologic conditions that are relevant to the central nervous system. Beyond the original role of statins in lowering cholesterol synthesis, they have anti-inflammatory, antioxidant and modulatory effects on signaling pathways. Additionally, Rho GTPase inhibitors and statins share the mevalonate pathway as a common target of their therapeutic actions. In this review, we discuss potential mechanisms through which these drugs, via their role in the mevalonate pathway, exert their neuroprotective effects in neurodegenerative and neurodevelopmental disorders., (Copyright© Bentham Science Publishers; For any queries, please email at epub@benthamscience.org.)
- Published
- 2017
- Full Text
- View/download PDF
23. [Spatial distribution pattern of main populations and gap makers in Picea koraiensis and Abies nephrolepis forest of Xiaoxing' an Mountains, Northeast China].
- Author
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Jing X, Duan WB, Chen LX, Wang T, Du S, Zhang YS, and Chen QM
- Subjects
- Abies, Acer, Betula, China, Picea, Population Density, Trees, Forests, Spatial Analysis
- Abstract
Species composition and diameter class structure were investigated in 1.5 hm2 (100 m x 150 n) permanent plot in Picea koraiensis and Abies nephrolepis forest of Xiaoxing' an Mountains. The spatial distribution pattern and spatial association of main populations and gap makers were analyzed by using point pattern analysis. The results showed that there were a total of 13 species with diameters at breast height greater than 2 cm in tree layer, and great differences were observed in the densities of main populations. The importance values of A. nephrolepis, P. koraiensis, Betula platyphylla and Acer ukurunduense were ranked in the first 4 in the plot. The diameter class structure of their populations presented an inverse 'J' curve. The spatial distribution patterns for A. nephrolepis and P. koraiensis were similar, which changed from aggregated, random to uniform distribution with the spatial scale. For B. platyphylla, the distribution was aggregated at ≤40 m scale, and random at >40 m scale, whereas A. ukurunduense presented an aggregated distribution pattern at the whole research scale. Except that the negative correlation between B. platyphylla and A. ukurunduense existed at the whole research scale, positive correlation between the other populations at small scale and negative correlation at large scale were observed. Only A. nephrolepis and B. platyphylla had significant positive correlation, and generally no significant correlation existed between other populations. Spatial distribution pattern of gap makers was characterized as aggregated distribution at small and middle scales, and random distribution with increasing scale. Spatial point pattern of gap makers formed by uprooting exhibited unimodal type distribution, and random, aggregated, and uniform distribution also occurred. Spatial point pattern of gap makers formed by breaking overall presented a little fluctuation, random and aggregated distributions alternatively appeared at small scale, and random distribution happened at large scale. Their spatial correlation analysis indicated that significant positive correlation existed at ≤32 m scale, and non-significant negative correlation at >32 m scale.
- Published
- 2015
24. Liver transplantation for biliary atresia: A single-center study from mainland China.
- Author
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Li QG, Wan P, Zhang JJ, Chen QM, Chen XS, Han LZ, and Xia Q
- Subjects
- Biliary Atresia diagnosis, Biliary Atresia mortality, Child, Child, Preschool, China, Databases, Factual, Female, Graft Survival, Health Services Accessibility, Humans, Infant, Kaplan-Meier Estimate, Living Donors supply & distribution, Male, Postoperative Complications etiology, Retrospective Studies, Risk Factors, Time Factors, Treatment Outcome, Biliary Atresia surgery, Liver Transplantation adverse effects, Liver Transplantation methods, Liver Transplantation mortality
- Abstract
Aim: To summarize our single-center experience with liver transplantation (LT) for biliary atresia (BA)., Methods: From October 2006 to December 2012, 188 children with BA were analyzed retrospectively. The stage I group (from October 2006 to December 2010) comprised the first 74 patients, and the stage II group (from January 2011 to December 2012) comprised the remaining 114 patients. Finally, 123 liver transplants were performed in 122 (64.9%) patients, whereas 66 patients did not undergo LT due to denial by their parents or lack of suitable liver grafts. The selection of graft types depended on the patients' clinical status and whether a suitable living donor was available. The characteristics of patients in stages I and II were described, and the surgical outcomes of LT recipients were compared between the two stages. The Kaplan-Meier method was used to estimate the cumulative patient and graft survival rates, and the equality of survival distributions was evaluated using the log-rank test., Results: The 188 children consisted of 102 boys and 86 girls. Their ages ranged from 3 to 144 mo with a median of 8 mo. One hundred and fifteen (61.2%) patients were born in rural areas. Comparing stage I and stage II patients, the proportion of patients referred by pediatricians (43.2% vs 71.1%, respectively; P < 0.001) and the proportion of patients who previously received a Kasai procedure (KP) (32.4% vs 44.7%, respectively; P = 0.092) obviously increased, and significantly more parents were willing to treat their children with LT (73% vs 86%, respectively; P = 0.027). Grafts from living donors (102/122, 83.6%) were the most commonly used graft type. Surgical complications (16/25, 64.0%) were the main reason for posttransplant mortality. Among the living donor liver transplantation recipients (n = 102), the incidence of surgical complications was significantly reduced (34.1% vs 15.5%, respectively; P = 0.029) and survival rates of patients and grafts were greatly improved (81.8% vs 89.7%, respectively, at 1 year; 75.0% vs 87.8%, respectively, at 3 years; P = 0.107) from stage I to stage II., Conclusion: The status of surgical treatments for BA has been changing in mainland China. Favorable midterm outcomes after LT were achieved as centers gained greater technical experience.
- Published
- 2015
- Full Text
- View/download PDF
25. [Effect of protocol RS-99 for childhood rhabdomyosarcoma].
- Author
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Tang JY, Pan C, Xu M, Xue HL, Chen J, Dong L, Zhou M, Gu LJ, and Chen QM
- Subjects
- Adolescent, Child, Child, Preschool, Female, Follow-Up Studies, Humans, Infant, Male, Nasopharyngeal Neoplasms chemically induced, Nasopharyngeal Neoplasms pathology, Nasopharyngeal Neoplasms therapy, Neoplasm Staging, Pelvic Neoplasms pathology, Pelvic Neoplasms therapy, Radiotherapy, Rhabdomyosarcoma pathology, Rhabdomyosarcoma therapy, Treatment Outcome, Antineoplastic Combined Chemotherapy Protocols, Pelvic Neoplasms diagnosis, Rhabdomyosarcoma diagnosis
- Abstract
Objective: To develop a reasonable protocol for childhood rhabdomyosarcoma (RS) to improve the prognosis of this disease., Methods: The protocol RS-99, developed on the base of pathological examination, clinical staging and grouping was used for 30 RS patients, 15 male and 15 female, aged 53 months (15-180 months). For the low-risk patients the regimen VCP and IeV were used alternately for 6 treatment courses and then local radiotherapy was given, for the median and high risk patients, regimen AVCP and IEV were used alternately for 6 courses, local radiotherapy and selective operation were performed, and then DEV and IeV were used alternately for 6 courses, and for the high-risk patients DEV and IeV were used alternately for 18 courses in total and then hematopoietic stem cell transplantation was conducted., Results: Twenty-three of the 30 patients reached complete response (CR) 17 of which remained in CR for 37 (32-92) months, and 7 of the 30 patients achieved partial response (PR) but their condition worsened later. Relapse was seen in 6 patients 15 (7-38) months later. The 5 year event-free survival (EFS) rate was 47% and the overall survival (OS) rate was 52%. All the 10 stage I and II patients, 5 of the 8 stage III patients, and only 2 of the 12 stage IV patients still remained in CR., Conclusion: The protocol RS-99 is effective on the RS at stages I, II, and III, however, is ineffective on the disease at stage IV.
- Published
- 2009
26. [Diagnosis and treatment of inflammatory myofibroblastic tumor with malignant transformation].
- Author
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Zhang ZD, Yin MZ, Chen QM, and Wang YP
- Subjects
- Abdomen surgery, Abdominal Neoplasms drug therapy, Abdominal Neoplasms surgery, Adolescent, Antineoplastic Combined Chemotherapy Protocols therapeutic use, Cisplatin administration & dosage, Doxorubicin administration & dosage, Female, Humans, Methotrexate administration & dosage, Neoplasms, Muscle Tissue drug therapy, Neoplasms, Muscle Tissue surgery, Abdominal Neoplasms pathology, Cell Transformation, Neoplastic, Neoplasms, Muscle Tissue pathology
- Published
- 2007
27. Construction and characterization of chimeric BHIV (BIV/HIV-1) viruses carrying the bovine immunodeficiency virus gag gene.
- Author
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Zhu YX, Liu C, Liu XL, Qiao WT, Chen QM, Zeng Y, and Geng YQ
- Subjects
- Animals, Cattle, Cells, Cultured, Humans, Recombinant Fusion Proteins genetics, Transfection, AIDS Vaccines genetics, Acquired Immunodeficiency Syndrome prevention & control, Genes, gag genetics, HIV-1 genetics, Immunodeficiency Virus, Bovine genetics
- Abstract
Aim: To explore the possibility of the replacement of the gag gene between human immunodeficiency virus and bovine immunodeficiency virus, to achieve chimeric virions, and thereby gain a new kind of AIDS vaccine based on BHIV chimeric viruses., Methods: A series of chimeric BHIV proviral DNAs differing in the replacement regions in gag gene were constructed, and then were transfected into 293T cells. The expression of chimeric viral genes was detected at the RNA and protein level. The supernatant of 293T cell was ultra centrifuged to detect the probable chimeric virion. Once the chimeric virion was detected, its biological activities were also assayed by infecting HIV-sensitive MT4 cells., Results: Four chimeric BHIV proviral DNAs were constructed. Genes in chimeric viruses expressed correctly in transfected 293T cells. All four constructs assembled chimeric virions with different degrees of efficiency. These virions had complete structures common to retroviruses and packaged genomic RNAs, but the cleavages of the precursor Gag proteins were abnormal to some extent. Three of these virions tested could attach and enter into MT4 cells, and one of them could complete the course of reverse transcription. Yet none of them could replicate in MT4 cells., Conclusion: The replacement of partial gag gene of HIV with BIV gag gene is feasible. Genes in chimeric BHIVs are accurately expressed, and virions are assembled. These chimeric BHIVs (proviral DNA together with virus particles) have the potential to become a new kind of HIV/AIDS vaccine.
- Published
- 2005
- Full Text
- View/download PDF
28. [Construction and analysis of activity of an HIV-1/bovine immunodeficiency virus chimeric clone cDNA].
- Author
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Yang YS, Chen GM, Dong WP, Chen QM, Geng YQ, and Zeng Y
- Subjects
- AIDS Vaccines, Animals, Cattle, Cloning, Molecular, DNA, Complementary genetics, Genes, gag genetics, Genes, pol genetics, Humans, Recombinant Fusion Proteins metabolism, Transcription, Genetic, Transcriptional Activation, Transfection, Virus Replication, DNA, Viral genetics, Genes, tat genetics, HIV-1 genetics, Immunodeficiency Virus, Bovine genetics, Recombinant Fusion Proteins genetics
- Abstract
Objective: Chimeric human/bovine immunodeficiency virus (HBIV) cDNA was constructed by replacing HIV tat and LTR with bovine immunodeficiency virus (BIV) tat and LTR to study the activity of BIV tat and LTR in the chimerae., Methods: The target fragments of BIV tat, LTR and HIV gag, pol, env were respectively amplified by using PCR and sequentially inserted into pBluescript SK(+) vector. The chimeric clone was transfected into human MT4 cells. The transcript and gene expression of the HBIV chimeric virus were detected by using RT-PCR and a reverse transcriptase assay, respectively., Results: BIV tat mRNA and HIV gag mRNA were detected. The reverse transcriptase activity of the chimeric virus was analyzed in the fluctuation curve., Conclusions: In chimeric HBIV cDNA transfected MT?4 cells, BIV tat and HIV gag were transcripted. The reverse transcriptase of the chimeric virus had biological activity. These data suggest that in MT4 cells, BIV LTR had promoter activity and BIV tat had the function of transactivation in the chimeric virus. The study of the chimeric virus with infectivity is in progress.
- Published
- 2003
29. Sequencing of hepatitis C virus cDNA with polymerase chain reaction directed sequencing.
- Author
-
Wei L, Wang Y, Chen HS, and Tao QM
- Abstract
Aim: To explore a rapid and easy sequencing method for hepatitis C virus (HCV) genome and establish a new sequencing method in China., Methods: Polymerase chain reaction (PCR) was combined with a DNA sequencing technique. PCR products were purified by agarose gel electrophoresis, polyacrylamide gel electrophoresis (PAGE) and polyethylene glycol (PEG) respectively. Then, in the presence of a 5' labeling PCR primer, purified PCR products were directly sequenced. By this method, HCV NS5b cDNA from two HCV infected individuals (HC-42 and HC-49) were sequenced., Results: PCR directed sequencing worked best using PCR amplified DNA purified by electrophoresis as a sequencing template. When sequencing a large number of templates, the purification step can be bypassed by using a lower concentration of dNTPs (40 μmol of each dNTP) and primers (10 pmol of each primer) in the first stage of PCR. The aliquot of the first stage of PCR mixture was then directly used for amplification of chain terminated products but the sequencing ladders generated were of low intensity. Polyethylene glycol (PEG) could not remove nonspecific products of PCR, which affected the sequencing result to a certain extent and generated a background in sequencing ladders. Compared with the reported HCVJ and HC-C2, a new three nucleotide deletion was found in HC-42., Conclusion: PCR directed sequencing is a rapid, simple and effective method, especially for sequencing large samples. A three nucleotide deletion was first reported.
- Published
- 1997
- Full Text
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