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169 results on '"Chen, Ruiai"'

22. Evaluation of different combination of pam2CSK4, poly (I:C) and imiquimod enhance immune responses to H9N2 avian influenza antigen in dendritic cells and duck

Author

Zhang, Aiguo, primary, Li, Deyin, additional, Song, Chao, additional, Jing, Huiyuan, additional, Li, Hongfei, additional, Mi, Junxian, additional, Zhang, Guizhi, additional, Jin, Shuangxing, additional, Ren, Xiaoli, additional, Huangfu, Heping, additional, Shi, Dongmei, additional, and Chen, Ruiai, additional

Published
2022
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24. Construction and Evaluation of the Immunogenicity and Protective Efficacy of Recombinant Replication-Deficient Human Adenovirus-5 Expressing Genotype VII Newcastle Disease Virus F Protein and Infectious Bursal Disease Virus VP2 Protein.

Author

Xu, Ting, Xiong, Ting, Xie, Wenting, Wu, Jing, Liu, Xiao, Li, Guimin, Lv, Yadi, Li, Linyu, Yang, Zekun, Wang, Han, Liu, Dingxiang, and Chen, Ruiai

Subjects
NEWCASTLE disease virus, ADENOVIRUS diseases, VIRAL proteins, IMMUNE response, NEWCASTLE disease, RECOMBINANT viruses
Abstract

Newcastle disease (ND) and infectious bursal disease (IBD) are two key infectious diseases that significantly threaten the health of the poultry industry. Although existing vaccinations can effectively prevent and treat these two diseases through multiple immunizations, frequent immunization stresses significantly impact chicken growth. In this study, three recombinant adenoviruses, rAd5-F expressing the NDV (genotype VII) F protein, rAd5-VP2 expressing the IBDV VP2 protein, and rAd5-VP2-F2A-F co-expressing F and VP2 proteins, were constructed using the AdEasy system. The F and VP2 genes of the recombinant adenoviruses could be transcribed and expressed normally in HEK293A cells as verified by RT-PCR and Western blot. The three recombinant viruses were shown to have similar growth kinetics as rAd5-EGFP. Compared with the PBS and rAd5-EGFP groups, SPF chickens immunized with recombinant adenoviruses produced higher antibody levels, more significant lymphocyte proliferation, and significantly higher CD4+/CD3+ and CD8+/CD3+ cells in peripheral blood. The survival rate of SPF chickens immunized with rAd5-F and rAd5-VP2-F2A-F after the challenge with DHN3 was 100%, and 86% of SPF chickens showed no viral shedding at 7 dpc. The survival rate of SPF chickens immunized with rAd5-VP2 and rAd5-VP2-F2A-F after the challenge with BC6/85 was 86%. rAd5-VP2 and rAd5-VP2-F2A-F significantly inhibited bursal atrophy and pathological changes compared to the rAd5-EGFP and PBS groups. This study provides evidence that these recombinant adenoviruses have the potential to be developed into safe and effective vaccine candidates for the prevention and control of ND and IBD. [ABSTRACT FROM AUTHOR]

Published
2023
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29. Expression and characterization of albumin fusion protein canine IFNγ-CSA in baculovirus-insect cell expression system

Author

Man-Lin Luo, Wu Jiajun, Anni Chen, Zou Shuzhan, Bu Wandi, Bing Li, Chen Ruiai, and Junqiong Zhai

Subjects
0106 biological sciences, Recombinant Fusion Proteins, medicine.medical_treatment, Serum albumin, Gene Expression, Sf9, Spodoptera, Antiviral Agents, 01 natural sciences, Vesicular stomatitis Indiana virus, Interferon-gamma, Mice, 03 medical and health sciences, Dogs, Pharmacokinetics, 010608 biotechnology, Sf9 Cells, medicine, Animals, Serum Albumin, 030304 developmental biology, 0303 health sciences, biology, Chemistry, Albumin, biology.organism_classification, Fusion protein, Molecular biology, Cytokine, Vesicular stomatitis virus, biology.protein, Female, Baculoviridae, Linker, Biotechnology
Abstract

In this study, canine IFNγ was fused by a flexible linker with canine serum albumin to construct the fusion protein IFNγ-CSA for the purpose to design a long-acting canine IFNγ. The fusion protein was successfully expressed in baculovirus-infected Sf9 insect cells and was purified by salting-out and ion exchange chromatography. The IFNγ-CSA fusion possessed potent anti-viral assay against vesicular stomatitis virus in cultured cells. IFNγ-CSA was also stable at 37 °C up to 72 h compared with 8 h for IFNγ alone. In vivo pharmacokinetics demonstrated a significantly longer half-life for IFNγ-CSA (15.42 h) than for canine reIFNγ (1.51 h) in KM mice. These results indicate that IFNγ-CSA expression in the baculovirus system was successful and provide a promising long-acting cytokine for veterinary clinical applications.

Published
2019

35. Albumin fusion improves the pharmacokinetics and in vivo antitumor efficacy of canine interferon gamma

Author

Zou Shuzhan, Wu Jiajun, Chen Ruiai, Man-Lin Luo, Anni Chen, He-Xing Wang, and Bing Li

Subjects
Recombinant Fusion Proteins, Serum albumin, Mice, Nude, Pharmaceutical Science, Antineoplastic Agents, Apoptosis, 02 engineering and technology, Pharmacology, 030226 pharmacology & pharmacy, Interferon-gamma, 03 medical and health sciences, 0302 clinical medicine, Pharmacokinetics, In vivo, Interferon, Neoplasms, medicine, Animals, Interferon gamma, Rats, Wistar, Serum Albumin, Cell Proliferation, Mice, Inbred BALB C, biology, Chemistry, Albumin, 021001 nanoscience & nanotechnology, Fusion protein, biology.protein, Female, 0210 nano-technology, medicine.drug
Abstract

Interferon (IFN)-γ plays an important role in antiviral, anti-proliferative, immunomodulatory and pro-inflammatory activities. However, the short therapeutic half-life of IFN-γ lessens its efficacy. Albumin fusion strategy is one of the most effective ways to improve the pharmacokinetic properties of cytokines. In this study, N- and C-terminal canine albumin fusions with canine IFN-γ were expressed in the baculovirus expression system. The fusion proteins stimulated Stat1 phosphorylation at levels similar to that of the recombinant IFN. The antiviral, anti-proliferative and promote apoptosis activity of CSA-IFN-γ was lower than IFN-γ-CSA and both were less than that of recombinant IFN-γ. In vivo pharmacokinetics demonstrated a significantly longer half-life for CSA-IFN-γ (21.73 h) than for IFN-γ-CSA (6.51 h) and canine reIFN-γ (2.22 h) in Wistar rats. CSA-IFN-γ was also more effective than IFN-γ-CSA and canine reIFN-γ at inhibiting growth of canine renal malignant histiocytosis in nude mice. Our results indicated that a canine serum albumin fusion at the N-terminus of IFN-γ prolongs its half-life and improves its in vivo antitumor activity.

Published
2019

41. A novel spike subunit 1‐based enzyme‐linked immunosorbent assay reveals widespread porcine torovirus infection in eastern China.

Author

Qin, Pan, Yang, Yong‐Le, Hu, Zhang‐Min, Zhang, Yu‐Qi, Mei, Xiao‐Qiang, Liang, Qi‐Zhang, Lu, Zongji, Wang, Bin, Chen, Ruiai, and Huang, Yao‐Wei

Subjects
ENZYME-linked immunosorbent assay, SOWS, SWINE, SWINE breeding, PIGLETS
Abstract

Toroviruses (ToVs), closely related but genetically distinct from coronaviruses, are known to infect horses, cows, pigs, goats and humans, mainly causing enteritic disorders. However, due to the lack of an adaptive culture system, porcine ToV (PToV) has received less attention. In this study, we developed a novel serological detection method based on the PToV envelope spike subunit 1 (S1) protein for the first time, and compared it to an existing indirect enzyme‐linked immunosorbent assay (ELISA) based on the nucleocapsid protein. By using the S1‐based ELISA, we carried out the first seroepidemiological survey of PToV in China, assaying both specific IgG and IgA responses in 1,037 serum samples collected from diarrheic pigs in eastern China. There was a relatively high incidence of seropositivity in pigs of different ages, especially one‐week‐old piglets and sows (78% and 43%), the former probably reflecting maternal antibodies. Furthermore, 3/20 (15%) of faecal samples collected from one PToV‐seropositive swine herd in Zhejiang province tested positive by RT‐PCR. The complete PToV genome was sequenced from one of these samples, and its phylogenetic relationship with other full‐length PToV sequences available in GenBank was determined. Our data provide the first serological evidence for PToV infection in pigs from China, which will help elucidate the potential pathogenicity of PToV in pigs. [ABSTRACT FROM AUTHOR]

Published
2022
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