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6. The individual contributions of blaB, blaGOB and blaCME on MICs of β-lactams in Elizabethkingia anophelis.

Author

Chen, Pei-Jing, Tan, Mei-Chen, Huang, Wei-Cheng, Hsu, Shu-Yuan, Chen, Te-Li, Yang, Chiou-Ying, and Kuo, Shu-Chen

Subjects
CEFTAZIDIME, CEFEPIME, GREATER wax moth, LACTAMS, DELETION mutation, PIPERACILLIN, CARBAPENEMS, ESCHERICHIA coli
Abstract

Background The bla B, bla GOB and bla CME genes are thought to confer β-lactam resistance to Elizabethkingia anophelis , based on experiments conducted primarily on Escherichia coli. Objectives To determine the individual contributions of β-lactamase genes to increased MICs in E. anophelis and to assess their impact on the in vivo efficacy of carbapenem therapy. Methods Scarless gene deletion of one or more β-lactamase gene(s) was performed in three clinical E. anophelis isolates. MICs were determined by broth microdilution. Hydrolytic activity and expressions of β-lactamase genes were measured by an enzymatic assay and quantitative RT–PCR, respectively. In vivo efficacy was determined using Galleria mellonella and murine thigh infection models. Results The presence of bla B resulted in >16-fold increases, while bla GOB caused 4–16-fold increases of carbapenem MICs. Hydrolysis of carbapenems was highest in lysates of bla B-positive strains, possibly due to the constitutionally higher expression of bla B. Imipenem was ineffective against bla B-positive isolates in vivo in terms of improvement of the survival of wax moth larvae and reduction of murine bacterial load. The deletion of bla B restored the efficacy of imipenem. The bla B gene was also responsible for a >4-fold increase of ampicillin/sulbactam and piperacillin/tazobactam MICs. The presence of bla CME, but not bla B or bla GOB, increased the MICs of ceftazidime and cefepime by 8–16- and 4–8-fold, respectively. Conclusions The constitutionally and highly expressed bla B gene in E. anophelis was responsible for increased MICs of carbapenems and led to their poor in vivo efficacy. bla CME increased the MICs of ceftazidime and cefepime. [ABSTRACT FROM AUTHOR]

Published
2024
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7. Elizabethkingia anophelis outer membrane vesicles as a novel vaccine candidate against infection: insights into immune response and potential for passive immunity

Author

Yang, Ya-Sung, primary, Chen, Hung-Jui, additional, Chen, Xiao-Chun, additional, Tang, Hung-Jen, additional, Chang, Fang-Ju, additional, Huang, Yun-Ling, additional, Pan, Yu-Ling, additional, Kesavan, Dinesh Kumar, additional, Chen, Huan-Yuan, additional, Shang, Hung-Sheng, additional, Kuo, Shu-Chen, additional, Chen, Te-Li, additional, and Chiang, Ming-Hsien, additional

Published
2023
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49. Klebsiella pneumoniae in gastrointestinal tract and pyogenic liver abscess

Author

Fung, Chang-Phone, Lin, Yi-Tsung, Lin, Jung-Chung, Chen, Te-Li, Yeh, Kuo-Ming, Chang, Feng-Yee, Chuang, Han-Chuan, Wu, Hau-Shin, Tseng, Chih-Peng, and Siu, L. Kristopher

Subjects
Abdomen -- Abscess, Liver diseases -- Causes of -- Diagnosis -- Research, Klebsiella -- Identification and classification -- Research, Health
Abstract

Klebsiella pneumoniae has emerged as the predominant cause of pyogenic liver abscess in Taiwan and other countries in eastern and Southeast Asia (1-4). This condition is frequently associated with severe [...]

Published
2012
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