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Color recovery methods applicable for images under chromatic illuminant were examined using a finite-dimensional linear model of surface spectral reflectances. The spectral distribution of the illuminant was measured, while spectral power distribution of reflected lights were estimated from their tristimulus values and the maximum surface spectral reflectance. Color recovery was faster in the maximum-tristimulus-value method than in the maximum-spectral-value technique.

Abstract: Ex vivo generation of megakaryocytes from hematopoietic stem cells (HSCs) is crucial to HSC research and has important clinical potential for thrombocytopenia patients to rapid platelet reconstruction. In this study, factorial design and steepest ascent method were used to screen and optimize the effective cytokines (10.2ng/ml TPO, 4.3ng/ml IL-3, 15.0ng/ml SCF, 5.6ng/ml IL-6, 2.8ng/ml FL, 2.8ng/ml IL-9, and 2.8ng/ml GM-CSF) in megakaryocyte induction medium that facilitate ex vivo megakaryopoiesis from CD34+ cells. After induction, the maximum fold expansion for accumulated megakaryocytes was almost 5000-fold, and the induced megakaryocytes were characterized by analysis of gene expression, polyploidy and platelet activation ability. Furthermore, the combination of megakaryocyte induction medium and HSC expansion medium can induce and expand a large amount of functional megakaryocytes efficiently, and might be a promising source of megakaryocytes and platelets for cell therapy in the future. [Copyright &y& Elsevier]