39 results on '"Chen, Zefu"'
Search Results
2. SIGMA leverages protein structural information to predict the pathogenicity of missense variants
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Zhao, Hengqiang, Du, Huakang, Zhao, Sen, Chen, Zefu, Li, Yaqi, Xu, Kexin, Liu, Bowen, Cheng, Xi, Wen, Wen, Li, Guozhuang, Chen, Guilin, Zhao, Zhengye, Qiu, Guixing, Liu, Pengfei, Zhang, Terry Jianguo, Wu, Zhihong, and Wu, Nan
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- 2024
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3. Chewing Gum Cannot Reduce Postoperative Abdominal Pain and Nausea After Posterior Spinal Fusions in Patients With Adolescent Idiopathic Scoliosis: A Systematic Review and Meta-analysis of Randomized Controlled Trials
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Tong, Bingdu, Chen, Zefu, Li, Gaoyang, Zhang, Li, and Chen, Yaping
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- 2023
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4. Prediction of new vertebral compression fracture within 3 years after percutaneous vertebroplasty for osteoporotic vertebral compression fracture: Establishment and validation of a nomogram prediction model
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Nie, Mingxi, primary, Chen, Zefu, additional, Shi, Liang, additional, Cao, HongXia, additional, and Xu, Lei, additional
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- 2024
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5. A genotype-first analysis in a cohort of Mullerian anomaly
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Tian, Weijie, Chen, Na, Ye, Yang, Ma, Congcong, Qin, Chenglu, Niu, Yuchen, Xiaoxin, L., Zhao, Lina, Zhao, Hengqiang, Liang, Ze, Song, Shuang, Wang, Yuan, Chen, Zefu, Lin, Jiachen, Yan, Zihui, Duan, Jiali, Zhao, Sen, Zhang, Terry Jianguo, Qiu, Guixing, Wu, Zhihong, Wu, Nan, and Zhu, Lan
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- 2022
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6. Expanding the mutation and phenotype spectrum of MYH3-associated skeletal disorders
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Zhao, Sen, Zhang, Yuanqiang, Hallgrimsdottir, Sigrun, Zuo, Yuzhi, Li, Xiaoxin, Batkovskyte, Dominyka, Liu, Sen, Lindelöf, Hillevi, Wang, Shengru, Hammarsjö, Anna, Yang, Yang, Ye, Yongyu, Wang, Lianlei, Yan, Zihui, Lin, Jiachen, Yu, Chenxi, Chen, Zefu, Niu, Yuchen, Wang, Huizi, Zhao, Zhi, Liu, Pengfei, Qiu, Guixing, Posey, Jennifer E., Wu, Zhihong, Lupski, James R., Micule, Ieva, Anderlid, Britt-Marie, Voss, Ulrika, Sulander, Dennis, Kuchinskaya, Ekaterina, Nordgren, Ann, Nilsson, Ola, Zhang, Terry Jianguo, Grigelioniene, Giedre, and Wu, Nan
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- 2022
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7. Investigation of the Lipid-Lowering Activity and Mechanism of Three Extracts from Astragalus membranaceus , Hippophae rhamnoides L., and Taraxacum mongolicum Hand. Mazz Based on Network Pharmacology and In Vitro and In Vivo Experiments.
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Yang, Xue, Jia, Mingjie, Luo, Jiayuan, An, Yuning, Chen, Zefu, and Bao, Yihong
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HDL cholesterol ,LDL cholesterol ,ASTRAGALUS membranaceus ,OXIDANT status ,HIPPOPHAE rhamnoides - Abstract
Hyperlipidemia is a metabolic disorder characterized by abnormal lipid metabolism, resulting in lipid accumulation in the plasma. According to reports, medicinal and edible plants can reduce the risk of metabolic diseases such as hyperlipidemia. This study investigates the effects and mechanisms of Astragalus membranaceus extract (AME), Hippophae rhamnoides L. extract (HRE), and Taraxacum mongolicum Hand. Mazz extract (TME) on hyperlipidemia. Active compounds and potential gene targets of AME, HRE, and TME were screened using LC-MS and TCMSP databases, and hyperlipidemia targets were detected from the OMIM and DisGeNet databases. A drug-target pathway disease network was constructed through protein interactions, GO enrichment, and KEGG pathway analysis. Finally, the lipid-lowering effects of three extracts were validated through in vitro HepG2 cell and in vivo animal experiments. The results show that LC-MS and network pharmacology methodologies identified 41 compounds and 140 targets. KEGG analysis indicated that the PI3K-Akt and MAPK signaling pathways significantly treat hyperlipidemia with AHT. In vitro experiments have shown that AHT is composed of a ratio of AME:HRE:TME = 3:1:2. HepG2 cell and animal experiments revealed that AHT exhibits strong lipid-lowering and antioxidant properties, significantly regulating the levels of total cholesterol (TC), triglycerides (TG), high-density lipoprotein cholesterol (HDL-C), low-density lipoprotein cholesterol (LDL-C), superoxide dismutase (SOD), and total antioxidant capacity (T-AOC). It is worth noting that AHT can effectively downregulate the protein expression levels of p-AKT/AKT and p-PI3K/PI3K and upregulate the protein expression levels of p-AMPK/AMPK and SIRT1, verifying the results predicted by network pharmacology. This study presents a novel approach to utilizing these natural plant extracts as safe and effective treatments for hyperlipidemia. [ABSTRACT FROM AUTHOR]
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- 2024
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8. Neuritin accelerates Schwann cell dedifferentiation via PI3K/Akt/mTOR signalling pathway during Wallerian degeneration.
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Liu, Jingmin, Guan, Xin, Zheng, Shuai, Shi, Jiawei, Wang, Xiaobo, Shen, Zetao, Chen, Zefu, Liao, Congrui, and Zhang, Zhongmin
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PERIPHERAL nerve injuries ,SCHWANN cells ,NEURODEGENERATION ,CELL death ,SCIATIC nerve - Abstract
Neuritin, also known as candidate plasticity gene 15 (CPG15), was first identified as one of the activity‐dependent gene products in the brain. Previous studies have been reported that Neuritin induces neuritogenesis, neurite arborization, neurite outgrowth and synapse formation, which are involved in the development and functions of the central nervous system. However, the role of Neuritin in peripheral nerve injury is still unknown. Given the importance and necessity of Schwann cell dedifferentiation response to peripheral nerve injury, we aim to investigate the molecular mechanism of Neuritin steering Schwann cell dedifferentiation during Wallerian degeneration (WD) in injured peripheral nerve. Herein, using the explants of sciatic nerve, an ex vivo model of nerve degeneration, we provided evidences indicating that Neuritin vividly accelerates Schwann cell dedifferentiation. Moreover, we found that Neuritin promotes Schwann cell demyelination as well as axonal degeneration, phagocytosis, secretion capacity. In summary, we first described Neuritin acts as a positive regulator for Schwann cell dedifferentiation and WD after peripheral nerve injury. [ABSTRACT FROM AUTHOR]
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- 2024
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9. Design and Fatigue Life Analysis of the Rope-Clamping Drive Mechanism in a Knotter.
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Yin, Jianjun, Chen, Zefu, Lv, Shiyu, Wu, Han, Gao, Yansu, and Wu, Luning
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FATIGUE life ,STRUCTURAL reliability ,STRUCTURAL design ,DYNAMIC simulation ,COUPLINGS (Gearing) ,VIRTUAL prototypes - Abstract
A knotter is a core component for the automatic bundling of agricultural materials, and a knotter with double-fluted discs is one type. Currently, the research on knotters with double-fluted discs has gradually transitioned from structural design to reliability optimization. To address rope-clamping failures in the rope-clamping drive mechanisms in knotters, the specific failure position of the rope-clamping mechanism and the failure causes were analyzed first. The redesign of the rope-clamping drive mechanism in knotters with double synclastic fluted discs was proposed, including structure optimization and 3D modeling using the GearTrax/KISSsoft and SolidWorks software. A virtual prototype model of a knotter with a flexible rope was established by combining ANSYS with the ADAMS software. A rigid–flexible coupling dynamic simulation of the knotter was carried out using ADAMS, and the simulation results were used as the data input for the ANSYS nCode DesignLife module for the fatigue life simulation of the weak parts (the worm shaft) of the knotter. The operation test results for the rope-clamping drive mechanism indicate that the redesigned rope-clamping drive mechanism is reliable in transmission, with a rope-clamping success rate of 100%. The actual operation times for the worm shaft exceed the minimum fatigue life obtained through joint simulation. The applied joint simulation method has high simulation accuracy. [ABSTRACT FROM AUTHOR]
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- 2024
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10. Deciphering the mutational signature of congenital limb malformations
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Sun, Liying, Huang, Yingzhao, Zhao, Sen, Zhao, Junhui, Yan, Zihui, Guo, Yang, Lin, Mao, Zhong, Wenyao, Yin, Yuehan, Chen, Zefu, Zhang, Nan, Zhang, Yuanqiang, Zhao, Zongxuan, Li, Qingyang, Wang, Lianlei, Dong, Xiying, Li, Yaqi, Li, Xiaoxin, Qiu, Guixing, Zhang, Terry Jianguo, Wu, Zhihong, Tian, Wen, and Wu, Nan
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- 2021
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11. A 3216 μm 2 MOS-Based Temperature Sensor with a Wide Temperature Measurement Range and Linear Readout.
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Li, Hao, Yang, Zhao, Kong, Dezhu, Yin, Aiguo, Chen, Zefu, and Zhang, Peiyong
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DIGITAL electronics ,VOLTAGE references ,INTELLIGENT sensors ,POWER resources ,MIXED signal circuits - Abstract
This paper introduces an MOS-based intelligent temperature sensor with a linear readout. Compared with similar designs, the proposed sensor utilizes the DIBL effect to reduce the precision requirement for the voltage reference source and compensate for the temperature measurement range. A compact voltage reference circuit is introduced, which generates two reference voltage bases using only three transistors. In addition, the proposed digital readout circuit does not require a subtractor or a divider, further saving area. Fabricated in a 55 nm CMOS process, the proposed sensor occupies a compact area of 3216 μ m 2 . Post-simulation results show it has a maximum error of −0.52/+0.28 °C within the temperature range of −20 °C to 120 °C after two-point calibration. The power supply voltage range of the sensor is 0.8 to 1.8 V. It has a maximum voltage sensitivity of 5.7 °C/V and its power consumption is only 166 nW, with a power supply voltage of 0.8 V. [ABSTRACT FROM AUTHOR]
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- 2024
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12. A Threshold Voltage Deviation Monitoring Scheme of Bit Transistors in 6T SRAM for Manufacturing Defects Detection.
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Liu, Rui, Li, Hao, Yang, Zhao, Wang, Guantao, Chen, Zefu, and Zhang, Peiyong
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THRESHOLD voltage ,MANUFACTURING defects ,STATIC random access memory ,MONTE Carlo method ,STANDARD deviations ,TRANSISTORS ,RANDOM access memory ,COMPLEMENTARY metal oxide semiconductors - Abstract
Transistor random threshold voltage variations due to process fluctuations seriously affects the stability of Static Random Access Memory (SRAM). In this paper, a SRAM bit transistors threshold voltage $({Vth})$ deviation monitoring scheme and system is proposed. This scheme ingeniously achieves on-chip measurement of all transistors threshold voltages without altering compact SRAM bit array layout. Control signal strategies and Transistor ${Vth}$ Determination Circuit (TVDC) for different types of Devices Under Test (DUTs) have been proposed. The system is implemented using a 65 nm CMOS process with a core area of 0.01875mm2. Through Monte Carlo analysis, the Weighted Average (WA) difference of the proposed scheme and the direct measurement method is not more than 10mV, and the Root Mean Square Error (RMSE) difference is not more than 3mV. This system can also effectively detect the cell position of the transistor threshold voltage mismatch simulated by modifying the substrate voltage. For SRAM arrays of different scales, the method proposed in this paper has area efficiency and flexible reconfigurability. [ABSTRACT FROM AUTHOR]
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- 2024
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13. Molecular Diagnostic Yield of Exome Sequencing and Chromosomal Microarray in Short Stature
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Li, Qing, primary, Chen, Zefu, additional, Wang, Jie, additional, Xu, Kexin, additional, Fan, Xin, additional, Gong, Chunxiu, additional, Wu, Zhihong, additional, Zhang, Terry Jianguo, additional, and Wu, Nan, additional
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- 2023
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14. De novo variants in H3-3A and H3-3B are associated with neurodevelopmental delay, dysmorphic features, and structural brain abnormalities
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Okur, Volkan, Chen, Zefu, Vossaert, Liesbeth, Peacock, Sandra, Rosenfeld, Jill, Zhao, Lina, Du, Haowei, Calamaro, Emily, Gerard, Amanda, Zhao, Sen, Kelsay, Jill, Lahr, Ashley, Mighton, Chloe, Porter, Hillary M., Siemon, Amy, Silver, Josh, Svihovec, Shayna, Fong, Chin-To, Grant, Christina L., Lerner-Ellis, Jordan, Manickam, Kandamurugu, Madan-Khetarpal, Suneeta, McCandless, Shawn E., Morel, Chantal F., Schaefer, G. Bradley, Berry-Kravis, Elizabeth M., Gates, Ryan, Gomez-Ospina, Natalia, Qiu, Guixing, Zhang, Terry Jianguo, Wu, Zhihong, Meng, Linyan, Liu, Pengfei, Scott, Daryl A., Lupski, James R., Eng, Christine M., Wu, Nan, and Yuan, Bo
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- 2021
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15. Anti-Wear Design of the Knot-Tripping Mechanism and Knot-Tying Test for the Knotter.
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Lv, Shiyu, Chen, Yaming, Yin, Jianjun, Zhou, Maile, and Chen, Zefu
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PROBLEM solving - Abstract
Aiming to solve the problem of knot-tripping failure caused by severe wear between the spherical roller and planar cam of the knotter, this paper first establishes a calculation model of the spatial cam contour surface. The knot-tripping mechanism in the knotter is designed as a line-contact curved-surface cam mechanism, with the cutter arm swinging in accordance with sinusoidal acceleration. The design significantly reduces the contact stress between the cam and the roller, compared to the original knot-tripping mechanism. Additionally, it eliminates the impact between the spherical roller and the planar cam. Based on the Archard model, the calculation model for cam-roller wear in the knot-tripping mechanism has been derived and utilized for wear calculation. The wear test results of the knot-tripping mechanism with an aluminum cam show that the curved cam has a wear amount that is 43%, 56%, 46%, and 37% lower than that of the planar cam after tying the knot 200 times, 600 times, 1300 times, and 2000 times, respectively. Under the condition that the twine tension is set to 120 N, and the rotation speed of the fluted disc is 60 rpm, the deviations between the calculated value and the measured value of the wear amount of the curved cam are 9.48%, 6.01%, 7.27%, and 9.95%, respectively. This validates the accuracy of the spatial cam wear model and the correctness of the curved cam design. [ABSTRACT FROM AUTHOR]
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- 2023
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16. Effects of saponins isolated from Polygonatum sibiricum on H2O2-induced oxidative damage in RIN-m5F cells and its protective effect on pancreas
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Luo, Jiayuan, primary, Chen, Zefu, additional, Guo, Qingqi, additional, Chai, Yangyang, additional, and Bao, Yihong, additional
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- 2023
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17. Clinical Diagnosis of Bone Metastasis in Breast Cancer via a Deep Learning Based Multi-Modal Image Analysis
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Ming, Yue, primary, Chen, Zefu, additional, Li, Yao, additional, Zhao, Jihuai, additional, Wang, Fengdan, additional, Zhu, Yuanpeng, additional, Dong, Xiying, additional, Cai, Jihao, additional, Chen, Guilin, additional, Nie, Xiangyu, additional, Wu, Zhihong, additional, Zhang, Terry Jianguo, additional, Wu, Nan, additional, Ma, Boyuan, additional, Huo, Li, additional, and Ban, Xiaojuan, additional
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- 2023
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18. Polygonatum sibiricum saponin Exerts Beneficial Hypoglycemic Effects in Type 2 Diabetes Mice by Improving Hepatic Insulin Resistance and Glycogen Synthesis-Related Proteins
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Chen, Zefu, primary, Luo, Jiayuan, additional, Jia, Mingjie, additional, Chai, Yangyang, additional, and Bao, Yihong, additional
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- 2022
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19. Functional characteristics of a broad spectrum of TBX6 variants in Mayer-Rokitansky-Küster-Hauser syndrome
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Ma, Congcong, primary, Chen, Na, additional, Jolly, Angad, additional, Zhao, Sen, additional, Coban-Akdemir, Zeynep, additional, Tian, Weijie, additional, Kang, Jia, additional, Ye, Yang, additional, Wang, Yuan, additional, Koch, André, additional, Zhang, Yuanqiang, additional, Qin, Chenglu, additional, Bonilla, Ximena, additional, Borel, Christelle, additional, Rall, Katharina, additional, Chen, Zefu, additional, Jhangiani, Shalini, additional, Niu, Yuchen, additional, Li, Xiaoxin, additional, Qiu, Guixing, additional, Zhang, Shuyang, additional, Luo, Guangnan, additional, Wu, Zhihong, additional, Bacopoulou, Flora, additional, Deligeoroglou, Efthymios, additional, Zhang, Terry Jianguo, additional, Rosenberg, Carla, additional, Gibbs, Richard A., additional, Dietrich, Jennifer E., additional, Dimas, Antigone S., additional, Liu, Pengfei, additional, Antonarakis, Stylianos E., additional, Brucker, Sara Y., additional, Posey, Jennifer E., additional, Lupski, James R., additional, Wu, Nan, additional, and Zhu, Lan, additional
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- 2022
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20. The utility of hierarchical genetic testing in paediatric liver disease
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Wang, Fuchuan, primary, Li, Yaqi, additional, Zhao, Sen, additional, Chen, Zefu, additional, Xu, Zhiqiang, additional, Wang, Lianlei, additional, Zhang, Terry Jianguo, additional, Yan, Jianguo, additional, Cao, Lili, additional, Wang, Pu, additional, Li, Aiqin, additional, Zhong, Yanwei, additional, Wu, Zhihong, additional, Qi, Xiaolong, additional, Zhang, Min, additional, and Wu, Nan, additional
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- 2022
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21. Identification of Novel FBN2 Variants in a Cohort of Congenital Contractural Arachnodactyly
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Sun, Liying, primary, Huang, Yingzhao, additional, Zhao, Sen, additional, Zhong, Wenyao, additional, Shi, Jile, additional, Guo, Yang, additional, Zhao, Junhui, additional, Xiong, Ge, additional, Yin, Yuehan, additional, Chen, Zefu, additional, Zhang, Nan, additional, Zhao, Zongxuan, additional, Li, Qingyang, additional, Chen, Dan, additional, Niu, Yuchen, additional, Li, Xiaoxin, additional, Qiu, Guixing, additional, Wu, Zhihong, additional, Zhang, Terry Jianguo, additional, Tian, Wen, additional, and Wu, Nan, additional
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- 2022
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22. PhenoApt leverages clinical expertise to prioritize candidate genes via machine learning
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Chen, Zefu, primary, Zheng, Yu, additional, Yang, Yongxin, additional, Huang, Yingzhao, additional, Zhao, Sen, additional, Zhao, Hengqiang, additional, Yu, Chenxi, additional, Dong, Xiying, additional, Zhang, Yuanqiang, additional, Wang, Lianlei, additional, Zhao, Zhengye, additional, Wang, Shengru, additional, Yang, Yang, additional, Ming, Yue, additional, Su, Jianzhong, additional, Qiu, Guixing, additional, Wu, Zhihong, additional, Zhang, Terry Jianguo, additional, and Wu, Nan, additional
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- 2022
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23. Clinical Characteristics of Chromobacterium Violaceum Infection
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Chen, Zefu, primary, Wu, Hua, additional, Wan, Kecheng, additional, Liang, Hong, additional, Sun, Song, additional, Ji, Xunqi, additional, Li, Shiguang, additional, Wenxiao, Yuan, additional, zheng, Pimei, additional, and Sui, Mingze, additional
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- 2022
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24. Expanding the mutation and phenotype spectrum of MYH3-associated skeletal disorders
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Zhao, Sen, Zhang, Yuanqiang, Hallgrimsdottir, Sigrun, Zuo, Yuzhi, Li, Xiaoxin, Batkovskyte, Dominyka, Liu, Sen, Lindelof, Hillevi, Wang, Shengru, Hammarsjo, Anna, Yang, Yang, Ye, Yongyu, Wang, Lianlei, Yan, Zihui, Lin, Jiachen, Yu, Chenxi, Chen, Zefu, Niu, Yuchen, Wang, Huizi, Zhao, Zhi, Liu, Pengfei, Qiu, Guixing, Posey, Jennifer E., Wu, Zhihong, Lupski, James R., Micule, Ieva, Anderlid, Britt-Marie, Voss, Ulrika, Sulander, Dennis, Kuchinskaya, Ekaterina, Nordgren, Ann, Nilsson, Ola, Zhang, Terry Jianguo, Grigelioniene, Giedre, and Wu, Nan
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Medical Genetics ,Medicinsk genetik - Abstract
Pathogenic variants in MYH3 cause distal arthrogryposis type 2A and type 2B3 as well as contractures, pterygia and spondylocarpotarsal fusion syndromes types 1A and 1B. These disorders are ultra-rare and their natural course and phenotypic variability are not well described. In this study, we summarize the clinical features and genetic findings of 17 patients from 10 unrelated families with vertebral malformations caused by dominant or recessive pathogenic variants in MYH3. Twelve novel pathogenic variants in MYH3 (NM_002470.4) were identified: three of them were de novo or inherited in autosomal dominant way and nine were inherited in autosomal recessive way. The patients had vertebral segmentation anomalies accompanied with variable joint contractures, short stature and dysmorphic facial features. There was a significant phenotypic overlap between dominant and recessive MYH3-associated conditions regarding the degree of short stature as well as the number of vertebral fusions. All monoallelic variants caused significantly decreased SMAD3 phosphorylation, which is consistent with the previously proposed pathogenic mechanism of impaired canonical TGF-beta signaling. Most of the biallelic variants were predicted to be protein-truncating, while one missense variant c.4244T>G,p.(Leu1415Arg), which was inherited in an autosomal recessive way, was found to alter the phosphorylation level of p38, suggesting an inhibition of the non-canonical pathway of TGF-beta signaling. In conclusion, the identification of 12 novel pathogenic variants and overlapping phenotypes in 17 affected individuals from 10 unrelated families expands the mutation and phenotype spectrum of MYH3-associated skeletal disorders. We show that disturbances of canonical or non-canonical TGF-beta signaling pathways are involved in pathogenesis of MYH3-associated skeletal fusion (MASF) syndrome. Funding Agencies|National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [81930068, 81772299, 81822030, 82072391, 81972132, 81672123, 81972037, 81902178]; Beijing Natural Science FoundationBeijing Natural Science Foundation [JQ20032, 7191007]; CAMS Innovation Fund for Medical Sciences (CIFMS) [2021-I2M-1-051, 2021-I2M-1-052]; Non-profit Central Research Institute Fund of Chinese Academy of Medical Sciences [2019PT320025]; Tsinghua University-Peking Union Medical College Hospital Initiative Scientific Research Program; PUMC Youth Fund & the Fundamental Research Funds for the Central Universities [3332019021]; Swedish Research CouncilSwedish Research CouncilEuropean Commission [K2015-54X-22 736-01-4, 2015-02227, 2018-03046]; Swedish Governmental Agency for Innovation Systems (Vinnova)Vinnova [2014-01438]; Marianne and Marcus Wallenberg Foundation; IngaBritt och Arne Lundbergs forskningsstiftelse; Byggmastare Olle Engkvist Stiftelse; Promobilia; Nyckelfonden; Stiftelsen Frimurare Barnhuset i Stockholm; Region Stockholm; Karolinska Institutet, Stockholm, Sweden; orebro University, orebro, Sweden; Sallskapet Barnavard; Karolinska InstitutetKarolinska Institutet; Stiftelsen Sallsyntafonden; Stiftelsen Samariten; Stiftelsen Promobilia; Region Stockholm [20180131, 20200500]; US National Institutes of Health (NIH), National Institute of Neurological Disorders and Stroke [NINDS R35 NS105078]; National Human Genome Research Institute/National Heart, Lung, and Blood Institute [NHGRI/NHLBI UM1 HG006542]; US NIH National Human Genome Research Institute [NHGRI K08 HG008986]
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- 2021
25. Disruptive NADSYN1 Variants Implicated in Congenital Vertebral Malformations
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Lin, Jiachen, primary, Zhao, Lina, additional, Zhao, Sen, additional, Li, Shengjie, additional, Zhao, Zhengye, additional, Chen, Zefu, additional, Zheng, Zhifa, additional, Shao, Jiashen, additional, Niu, Yuchen, additional, Li, Xiaoxin, additional, Zhang, Jianguo Terry, additional, Wu, Zhihong, additional, and Wu, Nan, additional
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- 2021
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26. Perturbations of genes essential for Müllerian duct and Wölffian duct development in Mayer-Rokitansky-Küster-Hauser syndrome
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Chen, Na, primary, Zhao, Sen, additional, Jolly, Angad, additional, Wang, Lianlei, additional, Pan, Hongxin, additional, Yuan, Jian, additional, Chen, Shaoke, additional, Koch, André, additional, Ma, Congcong, additional, Tian, Weijie, additional, Jia, Ziqi, additional, Kang, Jia, additional, Zhao, Lina, additional, Qin, Chenglu, additional, Fan, Xin, additional, Rall, Katharina, additional, Coban-Akdemir, Zeynep, additional, Chen, Zefu, additional, Jhangiani, Shalini, additional, Liang, Ze, additional, Niu, Yuchen, additional, Li, Xiaoxin, additional, Yan, Zihui, additional, Wu, Yong, additional, Dong, Shuangshuang, additional, Song, Chengcheng, additional, Qiu, Guixing, additional, Zhang, Shuyang, additional, Liu, Pengfei, additional, Posey, Jennifer E., additional, Zhang, Feng, additional, Luo, Guangnan, additional, Wu, Zhihong, additional, Su, Jianzhong, additional, Zhang, Jianguo, additional, Chen, Eugenia Y., additional, Rouskas, Konstantinos, additional, Glentis, Stavros, additional, Bacopoulou, Flora, additional, Deligeoroglou, Efthymios, additional, Chrousos, George, additional, Lyonnet, Stanislas, additional, Polak, Michel, additional, Rosenberg, Carla, additional, Dingeldein, Irene, additional, Bonilla, Ximena, additional, Borel, Christelle, additional, Gibbs, Richard A., additional, Dietrich, Jennifer E., additional, Dimas, Antigone S., additional, Antonarakis, Stylianos E., additional, Brucker, Sara Y., additional, Lupski, James R., additional, Wu, Nan, additional, Zhu, Lan, additional, Zhang, Terry Jianguo, additional, Wang, Shengru, additional, Liu, Jiaqi, additional, Liu, Sen, additional, Zuo, Yuzhi, additional, Liu, Gang, additional, Yu, Chenxi, additional, Liu, Lian, additional, Shao, Jiashen, additional, Zhao, Hengqiang, additional, Wang, Huizi, additional, Liu, Bowen, additional, Cheng, Xi, additional, Lin, Jiachen, additional, Du, Huakang, additional, Li, Yaqi, additional, Song, Shuang, additional, Xie, Zhixin, additional, Zhao, Zhengye, additional, Zhao, Zhi, additional, Zheng, Zhifa, additional, and Huang, Yingzhao, additional
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- 2021
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27. The Utility of Hierarchical Genetic Testing in Pediatric Liver Disease
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Wang, Fuchuan, primary, Li, Yaqi, additional, Zhao, Sen, additional, Dong, Yi, additional, Chen, Zefu, additional, Xu, Zhiqiang, additional, Wang, Lianlei, additional, Zhu, Shishu, additional, Zhang, Terry Jianguo, additional, Yan, Jianguo, additional, Cao, Lili, additional, Wang, Pu, additional, Li, Aiqin, additional, Zhong, Yanwei, additional, Wu, Zhihong, additional, Qi, Xiaolong, additional, Zhang, Min, additional, and Wu, Nan, additional
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- 2021
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28. Phenotypic and genetic spectrum of isolated macrodactyly: somatic mosaicism of PIK3CA and AKT1 oncogenic variants
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Tian, Wen, primary, Huang, Yingzhao, additional, Sun, Liying, additional, Guo, Yang, additional, Zhao, Sen, additional, Lin, Mao, additional, Dong, Xiying, additional, Zhong, Wenyao, additional, Yin, Yuehan, additional, Chen, Zefu, additional, Zhang, Nan, additional, Zhang, Yuanqiang, additional, Wang, Lianlei, additional, Lin, Jiachen, additional, Yan, Zihui, additional, Yang, Xinzhuang, additional, Zhao, Junhui, additional, Qiu, Guixing, additional, Zhang, Jianguo, additional, Wu, Zhihong, additional, and Wu, Nan, additional
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- 2020
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29. Diagnostic yield and clinical impact of exome sequencing in early-onset scoliosis (EOS)
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Zhao, Sen, primary, Zhang, Yuanqiang, additional, Chen, Weisheng, additional, Li, Weiyu, additional, Wang, Shengru, additional, Wang, Lianlei, additional, Zhao, Yanxue, additional, Lin, Mao, additional, Ye, Yongyu, additional, Lin, Jiachen, additional, Zheng, Yu, additional, Liu, Jiaqi, additional, Zhao, Hengqiang, additional, Yan, Zihui, additional, Yang, Yongxin, additional, Huang, Yingzhao, additional, Lin, Guanfeng, additional, Chen, Zefu, additional, Zhang, Zhen, additional, Liu, Sen, additional, Jin, Lichao, additional, Wang, Zhaoyang, additional, Chen, Jingdan, additional, Niu, Yuchen, additional, Li, Xiaoxin, additional, Wu, Yong, additional, Wang, Yipeng, additional, Du, Renqian, additional, Gao, Na, additional, Zhao, Hong, additional, Yang, Ying, additional, Liu, Ying, additional, Tian, Ye, additional, Li, Wenli, additional, Zhao, Yu, additional, Liu, Jia, additional, Yu, Bin, additional, Zhang, Na, additional, Yu, Keyi, additional, Yang, Xu, additional, Li, Shugang, additional, Xu, Yuan, additional, Hu, Jianhua, additional, Liu, Zhe, additional, Shen, Jianxiong, additional, Zhang, Shuyang, additional, Su, Jianzhong, additional, Khanshour, Anas M, additional, Kidane, Yared H, additional, Ramo, Brandon, additional, Rios, Jonathan J, additional, Liu, Pengfei, additional, Sutton, V. Reid, additional, Posey, Jennifer E, additional, Wu, Zhihong, additional, Qiu, Guixing, additional, Wise, Carol A, additional, Zhang, Feng, additional, Lupski, James R, additional, Zhang, Jianguo, additional, and Wu, Nan, additional
- Published
- 2020
- Full Text
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30. Phenotypic and genetic spectrum of isolated macrodactyly: somatic mosaicism of PIK3CA and AKT1 oncogenic variants.
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Tian, Wen, Huang, Yingzhao, Sun, Liying, Guo, Yang, Zhao, Sen, Lin, Mao, Dong, Xiying, Zhong, Wenyao, Yin, Yuehan, Chen, Zefu, Zhang, Nan, Zhang, Yuanqiang, Wang, Lianlei, Lin, Jiachen, Yan, Zihui, Yang, Xinzhuang, Zhao, Junhui, Qiu, Guixing, Zhang, Jianguo, and Wu, Zhihong
- Subjects
MOSAICISM ,NUCLEOTIDE sequencing ,LEG ,GENE frequency ,EXOMES ,STATISTICAL correlation - Abstract
Background: Isolated macrodactyly is a severe congenital hand anomaly with functional and physiological impact. Known causative genes include PIK3CA, AKT1 and PTEN. The aim of this study is to gain insights into the genetics basis of isolated macrodactyly.Results: We enrolled 24 patients with isolated macrodactyly. Four of them were diagnosed with Proteus syndrome based on skin presentations characteristic to this disease. Targeted next-generation sequencing was performed using patients' blood and affected tissues. Overall, 20 patients carry mosaic PIK3CA pathogenic variants, i.e. p.His1047Arg (N = 7), p.Glu542Lys (N = 6), p.Glu545Lys (N = 2), p.His1047Leu (N = 2), p.Glu453Lys (N = 1), p.Gln546Lys (N = 1) and p.His1047Tyr (N = 1). Four patients who met the diagnostic criteria of Proteus syndrome carry mosaic AKT1 p.Glu17Lys variant. Variant allele frequencies of these mosaic variants obtained through next-generation sequencing range from 10 to 33%. In genotype-phenotype correlation analysis of patients with PIK3CA variant, we found that patients with the macrodactyly of the lower limbs tend to carry PIK3CA variants located in the helical domain (P = 0.005).Conclusions: Mosaic PIK3CA and AKT1 variants can be found in all of our samples with isolated macrodactyly. Insights into phenotypic and genetic spectrum of isolated macrodactyly may be helpful in perusing a more precise and effective management of isolated macrodactyly. [ABSTRACT FROM AUTHOR]- Published
- 2020
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31. Cost-effectiveness analysis of using the TBX6-associated congenital scoliosis risk score (TACScore) in genetic diagnosis of congenital scoliosis.
- Author
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Chen, Zefu, Yan, Zihui, Yu, Chenxi, Liu, Jiaqi, Zhang, Yanbin, Zhao, Sen, Lin, Jiachen, Zhang, Yuanqiang, Wang, Lianlei, Lin, Mao, Huang, Yingzhao, Li, Xiaoxin, Niu, Yuchen, Wang, Shengru, Wu, Zhihong, DISCO (Deciphering disorders Involving Scoliosis and COmorbidities) study group, Qiu, Guixing, Zhang, Jianguo, Wu, Nan, and Zhu, Lan
- Subjects
- *
HUMAN chromosome abnormality diagnosis , *SCOLIOSIS , *HOUSEHOLD budgets , *COMORBIDITY , *DECISION trees , *COST estimates - Abstract
Background: We previously reported a novel clinically distinguishable subtype of congenital scoliosis (CS), namely, TBX6-associated congenital scoliosis (TACS). We further developed the TBX6-associated CS risk score (TACScore), a multivariate phenotype-based model to predict TACS according to the patient's clinical manifestations. In this study, we aimed to evaluate whether using the TACScore as a screening method prior to performing whole-exome sequencing (WES) is more cost-effective than using WES as the first-line genetic test for CS.Methods: We retrospectively collected the molecular data of 416 CS patients in the Deciphering disorders Involving Scoliosis and COmorbidities (DISCO) study. A decision tree was constructed to estimate the cost and the diagnostic time required for the two alternative strategies (TACScore versus WES). Bootstrapping simulations and sensitivity analyses were performed to examine the distributions and robustness of the estimates. The economic evaluation considered both the health care payer and the personal budget perspectives.Results: From the health care payer perspective, the strategy of using the TACScore as the primary screening method resulted in an average cost of $1074.2 (95%CI: $1044.8 to $1103.5) and an average diagnostic duration of 38.7d (95%CI: 37.8d to 39.6d) to obtain a molecular diagnosis for each patient. In contrast, the corresponding values were $1169.6 (95%CI: $1166.9 to $1172.2) and 41.4d (95%CI: 41.1d to 41.7d) taking WES as the first-line test (P < 0.001). From the personal budget perspective, patients who were predicted to be positive by the TACScore received a result with an average cost of $715.1 (95%CI: $594.5 to $835.7) and an average diagnostic duration of 30.4d (95%CI: 26.3d to 34.6d). Comparatively, the strategy of WES as the first-line test was estimated to have significantly longer diagnostic time with an average of 44.0d (95%CI: 43.2d to 44.9d), and more expensive with an average of $1193.4 (95%CI: $1185.5 to $1201.3) (P < 0.001). In 100% of the bootstrapping simulations, the TACScore strategy was significantly less costly and more time-saving than WES. The sensitivity analyses revealed that the TACScore strategy remained cost-effective even when the cost per WES decreased to $8.8.Conclusions: This retrospective study provides clinicians with economic evidence to integrate the TACScore into clinical practice. The TACScore can be considered a cost-effective tool when it serves as a screening test prior to performing WES. [ABSTRACT FROM AUTHOR]- Published
- 2020
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32. Diagnostic yield and clinical impact of exome sequencing in early-onset scoliosis (EOS)
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Zhao, Sen, Zhang, Yuanqiang, Chen, Weisheng, Li, Weiyu, Wang, Shengru, Wang, Lianlei, Zhao, Yanxue, Lin, Mao, Ye, Yongyu, Lin, Jiachen, Zheng, Yu, Liu, Jiaqi, Zhao, Hengqiang, Yan, Zihui, Yang, Yongxin, Huang, Yingzhao, Lin, Guanfeng, Chen, Zefu, Zhang, Zhen, Liu, Sen, Jin, Lichao, Wang, Zhaoyang, Chen, Jingdan, Niu, Yuchen, Li, Xiaoxin, Wu, Yong, Wang, Yipeng, Du, Renqian, Gao, Na, Zhao, Hong, Yang, Ying, Liu, Ying, Tian, Ye, Li, Wenli, Zhao, Yu, Liu, Jia, Yu, Bin, Zhang, Na, Yu, Keyi, Yang, Xu, Li, Shugang, Xu, Yuan, Hu, Jianhua, Liu, Zhe, Shen, Jianxiong, Zhang, Shuyang, Su, Jianzhong, Khanshour, Anas M, Kidane, Yared H, Ramo, Brandon, Rios, Jonathan J, Liu, Pengfei, Sutton, V. Reid, Posey, Jennifer E, Wu, Zhihong, Qiu, Guixing, Wise, Carol A, Zhang, Feng, Lupski, James R, Zhang, Jianguo, and Wu, Nan
- Abstract
BackgroundEarly-onset scoliosis (EOS), defined by an onset age of scoliosis less than 10 years, conveys significant health risk to affected children. Identification of the molecular aetiology underlying patients with EOS could provide valuable information for both clinical management and prenatal screening.MethodsIn this study, we consecutively recruited a cohort of 447 Chinese patients with operative EOS. We performed exome sequencing (ES) screening on these individuals and their available family members (totaling 670 subjects). Another cohort of 13 patients with idiopathic early-onset scoliosis (IEOS) from the USA who underwent ES was also recruited.ResultsAfter ES data processing and variant interpretation, we detected molecular diagnostic variants in 92 out of 447 (20.6%) Chinese patients with EOS, including 8 patients with molecular confirmation of their clinical diagnosis and 84 patients with molecular diagnoses of previously unrecognised diseases underlying scoliosis. One out of 13 patients with IEOS from the US cohort was molecularly diagnosed. The age at presentation, the number of organ systems involved and the Cobb angle were the three top features predictive of a molecular diagnosis.ConclusionES enabled the molecular diagnosis/classification of patients with EOS. Specific clinical features/feature pairs are able to indicate the likelihood of gaining a molecular diagnosis through ES.
- Published
- 2021
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33. Occurrence conditions and process analysis on fluidization of valley type landslide: Example of the fluidization of landslide in Qiongshan Ravine, Danba
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Kong Jiming, Chen Zefu, and Song Shuzhi
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geography ,Multidisciplinary ,geography.geographical_feature_category ,Process analysis ,Flow (psychology) ,Landslide ,Fluidization ,Ravine ,Geomorphology ,Debris flow - Abstract
A big debris flow which was arosed by landslide occurrence at night on July 11, 2003 in Qiongshan Ravine, Danba, Sichuan Province. According to the research, debris flow in Qiongshan Ravine is arosed by the fluidization of landslide. It had been rained about 70 days before debris flow occurred. That made the sediments in the gully to near to saturated or saturated in water. Being affect by the steep geography, the sediments occurrence peristalsis to transform, parts of soil bodies start disintegrate. All the sediments become transforming with water inleakaging to sediments, and than foreside slope flow to gully quickly after becoming fluidization. And the sediments come into being the debris flow with the water of the gully joining.
- Published
- 2006
34. Criterions and measures of route selection of shallowly embedded long-distance oil and gas pipeline in mountain areas
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MA Qing-wen, Chen Zefu, Li Xiuzhen, Kong Jiming, and Wang Chenghua
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geography ,Multidisciplinary ,Traverse ,geography.geographical_feature_category ,business.industry ,Landform ,Pipeline (computing) ,Fossil fuel ,Oil and gas pipelines ,Mining engineering ,Climbing ,Steep slope ,business ,Selection (genetic algorithm) ,Geology - Abstract
According to the engineering investigation of long-distance oil and gas pipelines, the criterions and measures of route selection are drawn as follows: the flat landform is the first choice in route alignment. The foot of mountain is the first choice when the route passes by the valley. The route should pass by but the shady and deposited slope and not in sunny and erosive slope as possible as it can. The pipeline should be vertical to contour climbing and descending the mountain except steep slope. Tunnel can be used in crossing foothill. Perpendicularly traversing the river is better than beveling; the worst choice is to put the pipeline along the river. Bypass is the best choice in karsts area. The order of route selection should be pre-choosing, investigation, optimization and adjustment.
- Published
- 2006
35. Deep learning-based multimodal image analysis for cervical cancer detection.
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Ming, Yue, Dong, Xiying, Zhao, Jihuai, Chen, Zefu, Wang, Hao, and Wu, Nan
- Subjects
- *
IMAGE fusion , *DEEP learning , *POSITRON emission tomography computed tomography , *MULTIMODAL user interfaces , *IMAGE analysis , *CERVICAL cancer , *EARLY detection of cancer , *OBJECT recognition (Computer vision) - Abstract
• A unified framework to detect cervical cancer using multimodal medical images. • An adaptive multi-model method to fuse multimodal medical images. • PET/CT fusion is more beneficial for the detection of cervical cancer than using either of them on their own; for example, only using CT mode detection or only using PET mode detection is not better than using PET/CT fusion mode detection. Cervical cancer is the fourth most common cancer in women, and its precise detection plays a critical role in disease treatment and prognosis prediction. Fluorodeoxyglucose positron emission tomography and computed tomography, i.e., FDG-PET/CT and PET/CT, have established roles with superior sensitivity and specificity in most cancer imaging applications. However, a typical FDG-PET/CT analysis involves the time-consuming process of interpreting hundreds of images, and the intense image screening work has greatly hindered clinicians. We propose a computer-aided deep learning-based framework to detect cervical cancer using multimodal medical images to increase the efficiency of clinical diagnosis. This framework has three components: image registration, multimodal image fusion, and lesion object detection. Compared to traditional approaches, our adaptive image fusion method fuses multimodal medical images. We discuss the performance of deep learning in each modality, and we conduct extensive experiments to compare the performance of different image fusion methods with some state-of-the-art (SOTA) object-detection deep learning-based methods in images with different modalities. Compared with PET, which has the highest recognition accuracy in single-modality images, the recognition accuracy of our proposed method on multiple object detection models is improved by an average of 6.06%. And compared with the best results of other multimodal fusion methods, our results have an average improvement of 8.9%. [ABSTRACT FROM AUTHOR]
- Published
- 2022
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36. Comparison analysis of the burden and attributable risk factors of early-onset and late-onset colorectal cancer in China from 1990 to 2019.
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Gong M, Xia T, Chen Z, and Zhu Y
- Abstract
Objectives: The project intended to analyze the impact of burden and related risk factors of late-onset colorectal cancer (LOCRC) and early-onset colorectal cancer (EOCRC) in China, thus offering essential references for optimizing prevention and control strategies., Method: Global Burden of Disease Study was employed to describe burden changes of EOCRC and LOCRC in China during 1990-2019, containing the numbers of incidence, deaths, prevalence, and disability-adjusted life years (DALYs), and to compare attributable deaths and DALYs risk factors in varying age and sex segments., Results: The numbers and corresponding crude rates of incidence, deaths, prevalence, and DALYs of EOCRC and LOCRC in China during 1990-2019 demonstrated an upward trend across all age categories, with males being dramatically predominant. Overall, over time, the impact of a low-calcium diet and a low-fiber diet on mortality and DALY rates decreased, while the impact of other risk factors increased. In terms of gender, the risk factors affecting males changed greatly, with smoking, inadequate milk intake, and the low whole-grain diet being the main factors in 2019, while in 1990, the main factors were the low-calcium diet, smoking, and inadequate milk intake., Conclusion: The burden of colorectal cancer in China is concerning. Patients grouped by diagnostic age exhibit different characteristics, indicating the need for high-quality research in the future to achieve personalized medicine tailored to different population characteristics., (Copyright © 2024 Wolters Kluwer Health, Inc. All rights reserved.)
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- 2024
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37. Case report: Heterozygous variation in the IGHMBP2 gene leading to spinal muscular atrophy with respiratory distress type 1.
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Zhou C, Chen Z, Chen Q, and Feng X
- Abstract
A rare autosomal recessive genetic disease is spinal muscular atrophy with respiratory distress type 1 (SMARD 1; OMIM #604320), which is characterized by progressive distal limb muscle weakness, muscular atrophy, and early onset of respiratory failure. Herein, we report the case of a 4-month-old female infant with SMARD type 1 who was admitted to our hospital owing to unexplained distal limb muscle weakness and early respiratory failure. This report summarizes the characteristics of SMARD type 1 caused by heterozygous variation in the immunoglobulin mu DNA binding protein 2 (IGHMBP2) gene by analyzing its clinical manifestations, genetic variation characteristics, and related examinations, aiming to deepen clinicians' understanding of the disease, assisting pediatricians in providing medical information to parents and improving the decision-making process involved in establishing life support., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2024 Zhou, Chen, Chen and Feng.)
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- 2024
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38. Long-term outcome of percutaneous vertebroplasty versus conservative treatment for osteoporotic vertebral compression fractures: a retrospective cohort study with three-year follow-up.
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Chen Z, Xu L, Shi L, Cao H, and Nie M
- Abstract
Background: Osteoporotic vertebral compression fractures (OVCF) appear to be more common as the population ages. Previous studies have found that percutaneous vertebroplasty (PVP) can achieve better short-term clinical outcomes than conservative treatment (CT) for OVCF. However, the long-term outcomes of PVP compared with CT for OVCF has been rare explored. This study was designed to explore the clinical outcomes of PVP or CT within 3 years after OVCF., Methods: This study reviewed the clinical outcomes of patients who underwent PVP or CT for OVCF in a single center from January 2015 to December 2019. The back pain visual analogue scale (VAS), Oswestry disability index (ODI) and satisfaction rate were compared between the two groups at baseline, 1 week, 1 month, 3 months, 6 months, 12 months, 24 months and 36 months after treatment., Outcomes: The baseline data including gender, age, bone mineral density, body mass index, back pain VAS, and ODI were not significantly different between the two groups. The back pain VAS and ODI of CT patients were significantly higher than those of PVP group at 1 week, 1 month, 3 months, 6 months and 12 months after treatment. The satisfaction rate in the PVP group were significantly higher than those in the CT group at 1 week, 1 month, 3 months and 6 months after treatment. Subsequently, the back pain VAS and ODI showed no significant difference between the two groups at 24 and 36 months. In addition, there was no significant difference in treatment satisfaction between the two groups at 36 months. There was no significant difference in the rate of new vertebral compression fractures between the two groups within 36 months after treatment., Conclusion: The clinical outcomes within 12 months after PVP and patient satisfaction rate within 6 months after PVP were significantly higher than CT. However, during 12 months to 36 months, this advantage generated by PVP was gradually diluted over time. Compared with CT, the long-term effect of PVP on OVCF should not be overestimated., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2024 Chen, Xu, Shi, Cao and Nie.)
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- 2024
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39. Effects of saponins isolated from Polygonatum sibiricum on H 2 O 2 -induced oxidative damage in RIN-m5F cells and its protective effect on pancreas.
- Author
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Luo J, Chen Z, Guo Q, Chai Y, and Bao Y
- Subjects
- Mice, Animals, Hydrogen Peroxide metabolism, Oxidative Stress, Apoptosis, Polygonatum, Diabetes Mellitus, Type 2 metabolism, Saponins pharmacology, Saponins metabolism, Islets of Langerhans metabolism
- Abstract
The damage of islet cells caused by oxidative stress is closely related to diabetes. The aim of this study is to investigate the protective effect of saponins isolated from polygonatum sibiricum (PSS) on pancreas injury by using in vitro and in vivo models. The oxidative stress model of RIN-m5F cells induced by H
2 O2 was established. We found that PSS could decrease the apoptosis of RIN-m5F cells under oxidative stress. After PSS treatment, ROS and MDA levels in cells significantly decreased. Moreover, the levels of SOD and GSH were significantly increased. PSS could increase the insulin secretion level of cells under oxidative stress. The expression level of intracellular Bcl-2 increased, and the expression levels of Bax, caspase-3, caspase-8, and caspase-9 decreased significantly. In addition, the type 2 diabetes mouse model was established. The results showed that PSS had a protective effect on the injury of the pancreas in T2DM mice. PSS can relieve oxidative stress and high glucose-mediated pancreas cytotoxicity. PSS may be a promising candidate for diabetes intervention and functional foods., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023. Published by Elsevier Ltd.)- Published
- 2023
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