Search

Your search keyword '"Chen, Zhaochun"' showing total 39 results
Start Over Author "Chen, Zhaochun"
39 results on '"Chen, Zhaochun"'

1. Potent monoclonal antibodies neutralize Omicron sublineages and other SARS-CoV-2 variants

Author

Chen, Zhaochun, Zhang, Peng, Matsuoka, Yumiko, Tsybovsky, Yaroslav, West, Kamille, Santos, Celia, Boyd, Lisa F., Nguyen, Hanh, Pomerenke, Anna, Stephens, Tyler, Olia, Adam S., Zhang, Baoshan, De Giorgi, Valeria, Holbrook, Michael R., Gross, Robin, Postnikova, Elena, Garza, Nicole L., Johnson, Reed F., Margulies, David H., Kwong, Peter D., Alter, Harvey J., Buchholz, Ursula J., Lusso, Paolo, and Farci, Patrizia

Published
2022
Full Text
View/download PDF

14. Extremely potent monoclonal antibodies neutralize Omicron and other SARS-CoV-2 variants

Author

Chen, Zhaochun, primary, Zhang, Peng, additional, Matsuoka, Yumiko, additional, Tsybovsky, Yaroslav, additional, West, Kamille, additional, Santos, Celia, additional, Boyd, Lisa F., additional, Nguyen, Hanh, additional, Pomerenke, Anna, additional, Stephens, Tyler, additional, Olia, Adam S., additional, De Giorgi, Valeria, additional, Holbrook, Michael R., additional, Gross, Robin, additional, Postnikova, Elena, additional, Garza, Nicole L., additional, Johnson, Reed F., additional, Margulies, David H., additional, Kwong, Peter D., additional, Alter, Harvey J., additional, Buchholz, Ursula J., additional, Lusso, Paolo, additional, and Farci, Patrizia, additional

Published
2022
Full Text
View/download PDF

17. Distinct disease features in chimpanzees infected with a precore HBV mutant associated with acute liver failure in humans

Author

Chen, Zhaochun, primary, Engle, Ronald E., additional, Shen, Chen-Hsiang, additional, Zhao, Huaying, additional, Schuck, Peter W., additional, Danoff, Emily J., additional, Nguyen, Hanh, additional, Nishimura, Norihisa, additional, Bock, Kevin W., additional, Moore, Ian N., additional, Kwong, Peter D., additional, Purcell, Robert H., additional, Govindarajan, Sugantha, additional, and Farci, Patrizia, additional

Published
2020
Full Text
View/download PDF

27. Delayed treatment with W1-mAb, a chimpanzee-derived monoclonal antibody against protective antigen, reduces mortality from challenges with anthrax edema or lethal toxin in rats and with anthrax spores in mice

Author

Altaweel, Laith, primary, Chen, Zhaochun, additional, Moayeri, Mahtab, additional, Cui, Xizhong, additional, Li, Yan, additional, Su, Junwu, additional, Fitz, Yvonne, additional, Johnson, Syd, additional, Leppla, Stephen H., additional, Purcell, Robert, additional, and Eichacker, Peter Q., additional

Published
2011
Full Text
View/download PDF

32. Characterization of Chimpanzee/Human Monoclonal Antibodies to Vaccinia Virus A33 Glycoprotein and Its Variola Virus Homolog In Vitro and in a Vaccinia Virus Mouse Protection Model

Author

Chen, Zhaochun, primary, Earl, Patricia, additional, Americo, Jeffrey, additional, Damon, Inger, additional, Smith, Scott K., additional, Yu, Fujuan, additional, Sebrell, Andrew, additional, Emerson, Suzanne, additional, Cohen, Gary, additional, Eisenberg, Roselyn J., additional, Gorshkova, Inna, additional, Schuck, Peter, additional, Satterfield, William, additional, Moss, Bernard, additional, and Purcell, Robert, additional

Published
2007
Full Text
View/download PDF

35. Bacillus anthracisCapsular Conjugates Elicit Chimpanzee Polyclonal Antibodies That Protect Mice from Pulmonary Anthrax

Author

Chen, Zhaochun, Schneerson, Rachel, Lovchik, Julie A., Dai, Zhongdong, Kubler-Kielb, Joanna, Agulto, Liane, Leppla, Stephen H., and Purcell, Robert H.

Abstract

ABSTRACTThe immunogenicity of Bacillus anthraciscapsule (poly-?-d-glutamic acid [PGA]) conjugated to recombinant B. anthracisprotective antigen (rPA) or to tetanus toxoid (TT) was evaluated in two anthrax-naive juvenile chimpanzees. In a previous study of these conjugates, highly protective monoclonal antibodies (MAbs) against PGA were generated. This study examines the polyclonal antibody response of the same animals. Preimmune antibodies to PGA with titers of >103were detected in the chimpanzees. The maximal titer of anti-PGA was induced within 1 to 2 weeks following the 1st immunization, with no booster effects following the 2nd and 3rd immunizations. Thus, the anti-PGA response in the chimpanzees resembled a secondary immune response. Screening of sera from nine unimmunized chimpanzees and six humans revealed antibodies to PGA in all samples, with an average titer of 103. An anti-PA response was also observed following immunization with PGA-rPA conjugate, similar to that seen following immunization with rPA alone. However, in contrast to anti-PGA, preimmune anti-PA antibody titers and those following the 1st immunization were =300, with the antibodies peaking above 104following the 2nd immunization. The polyclonal anti-PGA shared the MAb 11D epitope and, similar to the MAbs, exerted opsonophagocytic killing of B. anthracis. Most important, the PGA-TT–induced antibodies protected mice from a lethal challenge with virulent B. anthracisspores. Our data support the use of PGA conjugates, especially PGA-rPA targeting both toxin and capsule, as expanded-spectrum anthrax vaccines.

Published
2015
Full Text
View/download PDF

37. Single-chain ribosome inactivating proteins from plants depurinate Escherichia coli23S ribosomal RNA

Author

Hartley, Martin R., Legname, Giuseppe, Osborn, Rupert, Chen, Zhaochun, and Lord, J.Michael

Abstract

The rRNA N-glycosidase activities of the catalytically active A chains of the heterodimeric ribosome inactivating proteins (RIPs) ricin and abrin, the single-chain RIPs dianthin 30, dianthin 32, and the leaf and seed forms of pokeweed antiviral protein (PAP) were assayed on E. coliribosomes. All of the single-chain RIPs were active on E. coliribosomes as judged by the release of a 243 nucleotide fragment from the 3′ end of 23S rRNA following aniline treatment of the RNA. In contrast, E. coliribosomes were refractory to the A chains of ricin and abrin. The position of the modification of 23S rRNA by dianthin 32 was determined by primer extension and found to be A 2660, which lies in a sequence that is highly conserved in all species.

Published
1991
Full Text
View/download PDF

39. Extremely potent monoclonal antibodies neutralize Omicron and other SARS-CoV-2 variants.

Author

Chen Z, Zhang P, Matsuoka Y, Tsybovsky Y, West K, Santos C, Boyd LF, Nguyen H, Pomerenke A, Stephens T, Olia AS, De Giorgi V, Holbrook MR, Gross R, Postnikova E, Garza NL, Johnson RF, Margulies DH, Kwong PD, Alter HJ, Buchholz UJ, Lusso P, and Farci P

Abstract

The ongoing coronavirus disease 2019 (COVID-19) pandemic, caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), has triggered a devastating global health, social and economic crisis. The RNA nature and broad circulation of this virus facilitate the accumulation of mutations, leading to the continuous emergence of variants of concern with increased transmissibility or pathogenicity 1 . This poses a major challenge to the effectiveness of current vaccines and therapeutic antibodies 1, 2 . Thus, there is an urgent need for effective therapeutic and preventive measures with a broad spectrum of action, especially against variants with an unparalleled number of mutations such as the recently emerged Omicron variant, which is rapidly spreading across the globe 3 . Here, we used combinatorial antibody phage-display libraries from convalescent COVID-19 patients to generate monoclonal antibodies against the receptor-binding domain of the SARS-CoV-2 spike protein with ultrapotent neutralizing activity. One such antibody, NE12, neutralizes an early isolate, the WA-1 strain, as well as the Alpha and Delta variants with half-maximal inhibitory concentrations at picomolar level. A second antibody, NA8, has an unusual breadth of neutralization, with picomolar activity against both the Beta and Omicron variants. The prophylactic and therapeutic efficacy of NE12 and NA8 was confirmed in preclinical studies in the golden Syrian hamster model. Analysis by cryo-EM illustrated the structural basis for the neutralization properties of NE12 and NA8. Potent and broadly neutralizing antibodies against conserved regions of the SARS-CoV-2 spike protein may play a key role against future variants of concern that evade immune control.

Published
2022
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results